roman paskulin presentation 2009 sayulita
TRANSCRIPT
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Ibogaine and cellular metabolism
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Traditional use of Iboga
enteogen - ritual drug oneirogen - awake
dreams psychostimulant and
tonic reconvalescence after
infectious diseases aphrodisiac
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Use of Iboga/ibogaine in the West
interruption of addiction to alcohol, opiates, nicotine in cocaine
spiritual growth and self-realization
holly sacrament use at extreme sports –
mountaineering interesting for army
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Ibogaine in medicine
1905: 10–30mg per day against asthenia
1939-1970: neuromuscular stimulant 8mg per pill against fatigue, depression and at reconvalescence after infectious diseases
1969: 4-5mg/kg as fantasy enhancer at psychotherapy
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Pharmacodynamics of ibogaine
immediate (acute) effect
prolonged effect
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Pharmacodynamics of ibogaine
effects last longer than the presence of ibogaine in the body
fat deposits of lipophillic metabolite cannot directly cause the effect for weeks or months
functional and structural changes are expected
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Composition of cell
•nucleic acids
•proteins
•polysaccharides
•lipids
•water
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Proteins
•structural •functional
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Structural proteins-cytoskeleton
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Functional proteins-receptor
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Functional proteins-enzyme
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Protein separation by 2-D electrophoresis
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Computer scan of gel
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Computer scan of gel
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“In silico” analysis of results
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Spots of interest
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Peptide mapping by mass spectrometry
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Identification of proteins by matching with data bases
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Induction of energy metabolism related enzymes in rat brain
Enzyme Fold
24 h
Fold
72 h
Mr(Da)/pI Score Peptide matching
Glyceraldehyde-3-phosphate dehydrogenase
1.13 3.21 36090/8.14 62 9
Malate dehydrogenase
1.42 3.64 36117/8.79 54 9
Aldolase 1.23 2.45 39783/8.05 60 9
Pyruvate kinase 1.38 2.94 58314/7.19 70 10
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Induction of energy metabolism related enzymes
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Nonspecific activation of metabolism
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Interpretation of results
Enhanced energy availability supports higher metabolic turnover and by that facilitates detoxification and renewal of tissues.
Besides, consequent mental agility provides insight into one’s own psychological state
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Aura and ibogaine
-40 min +3 h
+54 h
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Aura and ibogaine
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Aura and ibogaine –thantanosis
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Aura and ibogaine
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Aura and ibogaine
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Aura and ibogaine
Maybe illumination and revelation are proper words to describe what is at this moment difficult to explain
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Pharmacodynamics of Entheogen Drugs - Influence on Gene Expression
- Faculty of Pharmacy, UL - Biotechnical Faculty, UL - Faculty of Medicine, UL
- Faculte de Pharmacie, Universite Montpellier 1, Montpellier, France
- Institute for Biological Research Siniša Stankovic, Belgrade, Serbia
OMI,Institute of Anthropological Medicine
www.omi.si