UNIVERSITI PUTRA MALAYSIA KAMPUS BINTULU (UPMKB)FACULTY OF AGRCULTURE AND FOOD SCIENCES

FMS 2901INDUSTRIAL TRAINING

INDIGENOUS FISHERIES REASEARCH AND PRODUCTION CENTRE (IFRPC)AGRICULTURE STATION OF TARAT, SERIAN

The Effect of Tubifex sp. as the Supplementary Feed on the Growth and Gonad Maturation of Tengadak (Barbonymus schwanenfeldii) Brood stock

Prepared by:Baiduri Mohamed

Matrics no.:S18417

Programme: Diploma of Fisheries, 6th Semester(2015/2016)

INTRODUCTION

Agriculture Station of Tarat, Serian is divided into two parts that is the agriculture and fishery.

The IFRPC aims to overcome the decline in the local river fish population.

4 areas for fishery sector. Among the species of fish available in this station are:

Empurau (Tor tambroides)Semah (Tor dourenensis)Tengadak (Barbonymus schwanenfeldii)Baung (Hemibagrus baramensis)

ACTIVITIES

Feed fish and Tubifex worm in tanks Cleaned and changed water in tanks Help staff to catch breeders for breeding Help in breeding of fishes: tengadak and baung Count baung and tengadak fry

Went to Sarawak Biodiversity Centre (SBC), Agriculture Research Centre Semenggok, Private Farm in Serikin and Puri Johan Agro Farm in Kg Taee.

Side project: Effect of feeding frequency on weight of guppies Help in preparation for Hari Peladang Penternak dan Nelayan Kebangsaan

(HPPNK) Help UNIMAS students to collect baung fry

Main Project

Effect of Tubifex as the Supplementary Feed on the Growth and Gonad Maturation of Tengadak

(Barbonymus schwanenfeldii) Brood Stocks

INTRODUCTION

Live feed such as Tubifex contains more nutritional value and have significantly affect the growth rate of C. batrachus larvae (Alam and Mollah 1988)

In this project, Tubifex worm is fed live along with commercial feed to tengadak brood stock.

This project was done for a period of 8 weeks (15th January – 11th March 2016)

Tengadak

Tengadak - Barbonymus schwanenfeldii Freshwater fish of the family Cyprinidae Grows up to 14 inches in length Quite similar to Java borb fish, Puntius gonionotus It has red dorsal fins with a black tip and two barbels at each end of the

upper lips. Benthopelagic Top 5 most expensive fish in Malaysia

Tubifex worm

Tubifex sp. is known as sludge worm Inhabit the sediments of lakes, rivers and sewer lines. As supplementary food either live or freeze dried Acts as feed ingredient and appetite stimulant in fishes High food values.

OBJECTIVES

To study the effect of Tubifex on as the supplementary diet on the growth and gonad maturation of Tengadak brood stocks

To study the growth performance of Tengadak brood stocks with different mixture of feed

To study the maturity of the reproductive organ of Tengadak brood stock

MATERIALS AND APPARATUS

9 pairs of tengadak brood

stock

9 HDPE tanks Culture of Tubifex Commercial fish feed

Multiparameter Dissolved oxygen meter

Digital weighing machine

Measuring apparatus

METHODOLOGY Fish are fed with 5% of its body weight Three treatments were used

Treatment 1 = 20% Tubifex + 80% pelletTreatment 2 = 40% Tubifex + 60% pelletTreatment 3 = 100% pellet

Each experimental feed are done in triplicates The design are randomized as follows:

- Treatment 1 (T1)

- Treatment 2 (T2)

- Treatment 3 (T3)

T1 T2 T3 T4 T5 T6 T7 T8 T9

Nine tanks were prepared by cleaning and filling with water. Aeration was placed

and flow through system was used

Taking initial weight and length

Placing 9 pairs of tengadak in 9 HDPE

tanks

The fish were acclimatised for 2

weeks and feed with commercial feed twice

a day.

The feed is slowly mixed with live

Tubifex for tanks 2 to 5, 7 and 9.

Tanks 1, 6 and 8 were fed with pellet. Tanks 2, 4 and 9 were fed

with T1 while tanks 3, 5 and 7 were fed with

T2.

Water quality is taken randomly every week.

Samplings were done every 2 or 3 weeks consist of weighing and measuring its

length.

RESULTS & DISCUSSIONS

The data for male of tank 7 on the 3rd week was unrecorded because only female tengadak was able to be caught.

As for tank 6, both tengadak were male on the 3rd and final sampling.

TankInitial (W0, g) 3rd week (W1, g) 6th week (W2, g) Final (W3, g)

Male Female Male Female Male Female Male Female

1 357 280.2 446 349 467 352 470 370

2 384 480 638 479 670 522 674 544

3 320.7 292.6 401 399 442 421 460 438

4 279 416.9 526 530 499 580 574 618

5 286.3 357 346 508 367 588 369 638

6 350.6 294.9 465 357478

no data 482

no data384 395

7 377.7 304.1 no data 381 457 357 453 374

8 287.2 351.8 367 463 387 518 384 540

9 369.2 297.8 536 336 548 352 538 355

Water quality

Date 10.2.2016 15.2.2016 17.2.2016 24.2.2016 29.2.2016 10.3.2016

Treatment pH DO pH DO pH DO pH DO pH DO pH DO

Treatment 1 7.5 2.4 8.1 3.3 8.5 2.2 7.5 4.5 8.0 2.0 7.1 2.9

Treatment 2 8.0 2.9 8.0 3.3 8.5 2.1 7.5 4.4 7.7 2.3 6.7 4.5

Treatment 3 7.3 2.5 8.2 8.1 2.1 7.4 4.3 7.5 7.5 2.5 6.8 4.1

Average weight gain

W0 W1 W2 W40

100

200

300

400

500

600

700

Average Weight Gain of Male Brood Stock

Treatment 1Treatment 2Treatment 3

W0 W1 W2 W30

100

200

300

400

500

600

Average Weight Gain of Female Brood Stock

Treatment 1Treatment 2Treatment 3

The average weight gain for both male and female tengadak is the highest when fed with treatment 1.

Average total length

TL0 TL1 TL2 TL326

27

28

29

30

31

32

33

34

Average Total Length of Male Brood Stock

Treatment 1Treatment 2Treatment 3

TL0 TL1 TL2 TL326

27

28

29

30

31

32

Average Total Length of Female Brood Stock

Treatment 1Treatment 2Treatment 3

Treatment 1 have the best effect on the average total length of tengadak brood stocks.

The average total length for male fed with treatment 2 decreases during week 3 because only 2 males were able to be caught

Average standard length

SL0 SL1 SL2 SL321.5

22

22.5

23

23.5

24

24.5

25

25.5

26

Average Standard Length of Male Brood Stock

Treatment 1Treatment 2Treatment 3

SL0 SL1 SL2 SL321

21.5

22

22.5

23

23.5

24

24.5

25

25.5

Average Standard Length of Female Brood Stock

Treatment 1Treatment 2Treatment 3

The highest average standard length pf brood stocks is when fed with treatment 1

During the final sampling, the females were observed for its egg. Most of the tengadak failed to spawn which may due to being out of its

spawning season (Richard Bailey and Brian Cole, 1999).

CONCLUSION

Growth of tengadak can be enhanced by feeding with Tubifex worm mixed with commercial feed.

Treatment 1 of 80% commercial feed and 20% Tubifex worm results in highest final average weight and length of tengadak brood stock.

Effect of Tubifex worm on gonad maturation was unable to be determined as no eggs were able to obtain from the female brood stock.

The project needs to be done in longer period not including the acclimatisation week to obtain the best result on its gonad maturation

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