sb bld 430 quiz 3 key
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8/2/2019 sb bld 430 quiz 3 key
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BLD 430 Quiz 3 Key
1. Organic solvents such as phenol can be used to extract nucleic acids.
A) True
B) False
2. Selection of a method to isolate DNA should be based on the sample requirements of
the method to be performed.
A) True
B) False
3. Parameters that influence what method to be used for nucleic acid isolation should
include, the quantity and purity of the isolated sample.
A) True
B) False
4. High molecular weight DNA has different base compositions than low molecularweight DNA.
A) True
B) False
5. If you have 5 mLs of peripheral blood, you can expect to isolate no more than 5mgs of
DNA.A) True
B) False
6. Buccal swabs are useful for obtaining samples to isolate DNA from.
A) TrueB) False
7. Most isolation methods will involve a cell lysis step.
A) True
B) False
8. DEPC works well to protect DNA during the isolation process.
A) True
B) False
9. A ratio of the 280/260 OD readings is a good estimate of DNA purity.A) True
B) False
10. A good way to estimate whether you have HMW dna is to run a gel.
A) True
B) False