8/2/2019 sb bld 430 quiz 6 key

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BLD 430 Quiz 6

1. Southern blots (RFLP) require HMW DNA.A) True

B) False

2. Probes for an RFLP can be DNA or RNA.

A) True

B) False

3. Probes for an RFLP assay can be labeled with a radio isotope or fluorochrome.A) True

B) False

4. RFLP need to have blocking reagents used to suppress background or non-specific

binding of the probe.

A) TrueB) False

5.Non-fat dried milk can be used as a blocking reagent.

A) True

B) False

6. The DNA that is electrophoresed on a gel to be used for an RFLP assay needs to be

denatured or made single stranded before transfer to a membrane.A) True

B) False

7. DNA can be denature or made single stranded by heat or NaOH.A) True

B) False

8. Restriction endoclease digested DNA from a human will appear as a streak on a gel

when electrophoresed.A) True

B) False

9. Restriction endonuclease digested DNA that was electrophoresed on a gel, transferred

to a membrane and probed is likely to give discrete bands based on their relative sizebased on the RE cuts and where the probe binds.

A) True

B) False

10. RFLP analysis can also be done using digested amplified fragments instead of

digested genomic DNA.A) True

8/2/2019 sb bld 430 quiz 6 key

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B) False