O

HHH

H

OHOH

H OH

OH

O

HHH

H

OHOH

H OH

OH

O

HHH

H

OHO

H OH

OH

O

HHH

H

OHO

H OH

O

O

HHH

H

OHO

H OH

OH

OH

n

Schematic layout of glucan molecule

Three-dimensional shape of glucan molecule

Baker’s yeast, Saccharomyces

cerevisiae viewed under the

microscope

A single whole yeast cell is about

1-3 microns in diameter.

The PAC MAN of the immune system in various states – mostly extending its ruffles and tentacles to devour any non-self that might be present…including some asbestos in the lower left & Rod-shaped bacilli like the top right photo

Mice were fed fluorescently labeled Whole Glucan Particles (WGPs) for 3

days Spleens were harvested at 3 days and

splenic macrophages isolated by FACS

Fluorescent microscopy demonstrates oral uptake of fluorescent WGPs into

splenic macrophages

3 days

Splenic Macrophage

Ross

Demonstration of Oral Uptake of Yeast Beta-1, 3-D glucan

Mice were fed fluorescently labeled WGPs for 12 days

Bone marrow was harvested at 12 days

and bone marrow macrophages isolated

by FACS

Fluorescent microscopy demonstrates oral uptake of fluorescent WGPs into bone

marrow macrophages

Distribution of Beta 1,3-D Glucan from the intestine throughout the reticuloendothelial system is mediated by macrophage trafficking

12 days

Bone Marrow Macrophages

Ross

Demonstration of Oral Uptake of Yeast Demonstration of Oral Uptake of Yeast Beta 1,3-D GlucanBeta 1,3-D Glucan

Days

Pg

of

IL-2

/ml

Long-term effect of orally-given glucans on secretion of IL-2 by spleen cellsLong-term effect of orally-given glucans on secretion of IL-2 by spleen cells

Cel

ls (

x106 )

Days after irradiation

Glucan-induced recovery of bone marrow after irradiation of miceGlucan-induced recovery of bone marrow after irradiation of mice

Mg/

dL

Days after cholesterol

Effect of long-term feeding with individual glucans on blood cholesterol levels in experimentally-induced hypercholesterolemia

Days after Streptozotocin

Per

cen

tag

e o

f co

ntr

ol

valu

es

Feeding with glucan can control the experimentally-induced hyperglycemiaFeeding with glucan can control the experimentally-induced hyperglycemia

% o

f in

hib

itio

n Effects of various glucans on ear swelling Effects of various glucans on ear swelling

% p

hago

cyto

sing

neu

trop

hils

Glucan in diet reduces suppression of immunity caused by mercuryGlucan in diet reduces suppression of immunity caused by mercury

Effects of different glucans on immunosuppression caused by immunotoxic agentsEffects of different glucans on immunosuppression caused by immunotoxic agents%

of

ph

ago

cyto

sin

g n

eutr

op

hil

s

Recoverage area (% of Control)

Glucan stimulates the wound healing of human skin cellsGlucan stimulates the wound healing of human skin cells

Skin dryness

Skin hydration Facial wrinkles

ng/ml

Effect of 14 days of feeding on stress-induced levels of corticosterone Effect of 14 days of feeding on stress-induced levels of corticosterone

mg

/LEffects of glucan supplementation of antibody formation in childrenEffects of glucan supplementation of antibody formation in children

% o

f p

os

itiv

e c

ells

Effects of different immunomodulators on phagocytic activity

Effects of various combinations on NK cell activityEffects of various combinations on NK cell activity

Mg of glucan

% p

hago

cyto

sis

More glucan does not always mean higher activity as inferior glucans never reach the biological activities of superior glucans

More glucan does not always mean higher activity as inferior glucans never reach the biological activities of superior glucans

pg o

f IL-

2

%

%

Effects of resveratrol-vitamin C-glucan combination on phagocytosis in stressed miceEffects of resveratrol-vitamin C-glucan combination on phagocytosis in stressed mice

BEFORE

AFTER

1 – People who:

Are undergoing chemoAre undergoing radiationAre undergoing surgeryAre getting vaccinatedAre under stressAre having issues with – heavy metals, toxins, chronic or acute infectionsAre concerned about the fluAre concerned about parasitesAre parents or caretakers of children or elderlyAre slow or incomplete healersNeed a great pro-bioticMay be marketing another ‘immune support” compound

2 – Find a free place to meet and advertise as a PUBLIC SERVICE 3 – Offer a free event to show 1 hour videos such as TTAC volume 2 4 – Keep ready your array or 3rd party information to do follow up 5 – Be honest, informed, relentless and indefatigable. 6 – Learn to get outside your circle and be a resource for reliable and irrefutable information*. - www.vitawithimmunity.com https://www.facebook.com/immunehealthbychoiceBeta Glucan – Nature’s Secret by Vaclav Vetvicka, PhD

HOW TO NEVER RUN OUT OF LEADS

We would like to acknowledge the following for either their sponsorship, work, introduction and participation in making this presentation available to you:

Helen Watt

Sheryl Morley

Special thanks to all the attendees

A.J. Lanigan will be available to answer questions

Acknowledgement