scpl17-1 wild type scpc17:scpl17 scpl17-1 scpl17-2 csmv:scpl17 scpl17-2 retention time (min)...

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2 4 6 8 10 12 14 2 4 6 8 10 12 14 scpl17-1 wild type SCPC17:SCPL17 scpl17-1 scpl17-2 CsMV:SCPL17 scpl17-2 Retention time (min) Retention time (min) Absorbance at 229 nm Absorbance at 330 nm 3BZO 4BZO 4SIN 3SIN scpl17-1 wild type SCPC17:SCPL17 scpl17-1 scpl17-2 CsMV:SCPL17 scpl17-2 IS (a ) (b ) Figure S1. Complementation analyses of scpl17 mutant seeds. Two independent scpl17 mutants were transformed with the constructs overexpressing SCPL17 under endogenous SCPL17 promoter and CsMV promoter. Total GSL profiles at 229 nm (a) and SnGSL profiles at 330 nm (b) are extracted from the same diode array dataset and the y-axis of each chromatogram is scaled equivalently. IS, internal standard sinigrin.

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Figure S3. Benzoate feeding into wild-type and bzo1-4 siliques. After 3 days, the amounts of benzoylglucose, benzoylcholine (A), OH-GSLs (B), and BzGSLs (C) were measured. - BA: siliques treated with DDW; + BA: siliques treated with 1mM benzoate. *, Student’s t-test, P < 0.05 vs. - BA. Mean±SD (n = 3). (a) (b) pmol mg tissue -1 nmol mg tissue -1 * * * (c) nmol mg tissue -1 * * * * *

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Page 1: Scpl17-1 wild type SCPC17:SCPL17 scpl17-1 scpl17-2 CsMV:SCPL17 scpl17-2 Retention time (min) Absorbance at 229 nm Absorbance at 330 nm 3BZO 4BZO4SIN 3SIN

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

scpl17-1

wild type

SCPC17:SCPL17scpl17-1

scpl17-2

CsMV:SCPL17scpl17-2

Retention time (min) Retention time (min)

Abs

orba

nce

at 2

29 n

m

Abs

orba

nce

at 3

30 n

m

3BZO 4B

ZO

4SIN

3SIN

scpl17-1

wild type

SCPC17:SCPL17scpl17-1

scpl17-2

CsMV:SCPL17scpl17-2

IS

(a) (b)

Figure S1. Complementation analyses of scpl17 mutant seeds. Two independent scpl17 mutants were transformed with the constructs overexpressing SCPL17 under endogenous SCPL17 promoter and CsMV promoter. Total GSL profiles at 229 nm (a) and SnGSL profiles at 330 nm (b) are extracted from the same diode array dataset and the y-axis of each chromatogram is scaled equivalently. IS, internal standard sinigrin.

Page 2: Scpl17-1 wild type SCPC17:SCPL17 scpl17-1 scpl17-2 CsMV:SCPL17 scpl17-2 Retention time (min) Absorbance at 229 nm Absorbance at 330 nm 3BZO 4BZO4SIN 3SIN

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Minutes2 4 6 8 10 12 14

Abs

orba

nce

at 2

29 n

m

Abs

orba

nce

at 3

30 n

m

sng2-2

wild type

CsMV:SCTsng2-2

3BZO 4B

ZO

sng2-2

wild type

CsMV:SCTsng2-2

Figure S2. Complementation analyses of sng2-2 mutant seeds. The sng2-2 mutant was complemented with the construct overexpressing SCT under CsMV promoter. Total GSL profiles at 229 nm (a) and SnGSL profiles at 330 nm (b) are extracted from the same diode array dataset and the y-axis of each chromatogram is scaled equivalently. IS, internal standard sinigrin.

4SIN

3SIN

Retention time (min) Retention time (min)

IS

(a) (b)

Page 3: Scpl17-1 wild type SCPC17:SCPL17 scpl17-1 scpl17-2 CsMV:SCPL17 scpl17-2 Retention time (min) Absorbance at 229 nm Absorbance at 330 nm 3BZO 4BZO4SIN 3SIN

Figure S3. Benzoate feeding into wild-type and bzo1-4 siliques. After 3 days, the amounts of benzoylglucose, benzoylcholine (A), OH-GSLs (B), and BzGSLs (C) were measured. - BA: siliques treated with DDW; + BA: siliques treated with 1mM benzoate. *, Student’s t-test, P < 0.05 vs. - BA. Mean±SD (n = 3).

(a)

(b)

pmol

mg

tissu

e-1nm

ol m

g tis

sue-1

*

*

*

(c)

nmol

mg

tissu

e-1

*

*

*

*

*

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