sealing - biotools · power: 300w sealing temp: 60-200°c sealing time: 0 - 10 seconds connection:...
TRANSCRIPT
You can choose between adhesive and heat sealing solutions. Both offer a wide selection of materials to choose from depending on your application requirements. See page 83 for application dependent selection of adhesive seals and page 73 for heat seals.
All 4titude® seals are certified free from nucleases and human genomic DNA.
Adhesive Seals:
Most adhesive seals are supplied with convenient tabs on both ends for easy application. These tabs also enable easy peeling to remove a seal without leaving adhesive residue on the sealing surface. For best sealing results we offer a roller, see page 53 to ensure even pressure is applied, for complete and secure application of all our adhesive seals.
Heat Seals:
Heat seals are available as sheets, for manual or semi-automatic heat sealers, as well as in roll formats for automated sealers. Heat sealing offers 100% effective sealing integrity and is a quick and cost-effective sealing solution.
Depending on throughput we recommend our semi-automatic 4s3 heat sealer page 64 or automated A4S sealer page 62 for applying your heat seal.
Sealing4titude® offers the widest range of plate sealing solutions on the market.
All seals are certified free from nucleases and human genomic DNA
| www.4ti.co.uk60 +44 (0)1306 884885 | www.4ti.co.uk60 +44 (0)1306 884885
Heat SealingHeat sealing is the gold standard plate sealing method, minimising sample loss and maximising sample security, for PCR, qPCR, storage and other applications.
IMAGES?
The sealing performance of heat seals is superior to cap, mat and adhesive sealing with clear reductions in sample loss and cross contamination. The optimized sealing performance allows use of smaller reaction volumes leading to reagent savings.
Heat sealing is the most cost efficient way to seal plates for a wide range of applications. Per well comparisons show a 3 to 5 five fold reduction in costs for qPCR application (list price comparison of Clear Weld Seal against caps and qPCR seal) and at least a 50% reduction for storage solutions (list price comparison of Peel Seal against caps and PCR seal). Costs can be further reduced by changing to use of the material format, using rolls rather than sheets.
The application of a heat seals is much faster than caps or adhesive seals. Typical sealing times are around 2,5 seconds. Depending on throughput the use of a semi-automatic or fully automatic heat sealer can guarantee a reproducible sealing quality by standardising the sealing parameters, time, temperature and pressure. Sealing performance variability between different users can be eliminated.
4titude® offers a wide choice of heat seals in both roll and sheet format. Different seals can be chosen to optimize the use depending on plate material, desired permeability or resistance, ability to peel or pierce the seal material and visualisation through the material. Please refer to the comparison table on page 73 for an overview of our heat sealing range.
All seals are certified free from nucleases and human genomic DNA contamination.
The best sealing results can be obtained by using flat, rigid plates with a raised rim design like our FrameStar® 2-component plates and reliable, high quality sealing instruments such as the 4s3 semi-automated heat sealer or the 4AS sealer.
For details of the instruments see page 62 and 64. For a demonstration or trial of a heat sealer please contact us at [email protected].
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Automatic Heat Sealer A4SThe A4S automatic heat sealer completes our heat sealing range by providing the perfect solution for automated heat sealing of medium to high-throughput or full automation applications.
Crucially the A4S does not require an air supply allowing for flexible use on standard lab benches as stand-alone unit, or integrated within a robotic set-up. The sealer has a rapid heating element, for fast start-up, and has a stand-by function, to save energy when not in use.
The A4S sealer works with a wide range of plates from 384well and 24well PCR plates, to deep well or storage plates with an SBS footprint. Different plate heights, from 3mm to 60mm, can be accommodated with minimal adjustments on the instrument. Plates are presented in portrait format and the plate position on the shuttle allows good access for robotic integration.
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Feature:
• No air supply needed
• Colour-Touch-screen/Institutive user interface
• Saved sealing protocols
• Auto stand-by function to save energy
• Compatible with a wide range of the plate types, including 1536well PCR plates
• Rapid heating element for fast start-up
• Optional cover
• 2 roll positions depending on available space and roll size
• Sealing cycle time of less than 15sec
• Up to 5000 seals without manual intervention
Standard size, 78mm width rolls, can be loaded in either of two roll positions depending on roll length and available space enabling the entire instrument to fit under the gantree of many liquid handling robots.
The A4S doesn’t produce any sealing material waste. The roll width covers an SBS footprint microplate and the seal is cut at the end of the plate leaving only minimal overhangs to allow for easy removal of the seal, but not interfering with plate stacking.
For our range of sealing materials see page 66 to 72. Sample rolls are available on request.
Technical Specification
Weight: 25 kg
Voltage: 110/230V
Maximum power consumption: 750W
Power saving mode: less than 20W
Sealing Temp: 120-200C
Sealing Time: 0-10sec
Connection: RS-232 serial port The modern user interface has a colour touchscreen, intuitive controls and can save individual sealing protocols for future use.
4ti-0665 A4S Automatic Sealer 1
Code Description Quantity
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Features and Benefits
• Automaticsealingprocessforunmatchedsealingconsistency
• Variabletemperatureandtimesettingsforeasyoptimisationofsealingconditions
• Simple,singlebuttonoperation
• Rapidheatingelementforfaststart-up
• Real-timetemperaturedisplay
• Autostand-byfunctiontosaveenergy
• Autoswitch-offfunctionforaddedsafety
• Compatiblewithwidevareityofconsumables
• RS-232serialportenablesunittobeintegratedintoautomatedsystems
• Fullrangeofheatsealsavailable
• Smallfootprint
• Lowtemperaturesealingforusewithadhesiveseals
Comparedtolessautomatedheatsealersonthemarketthe4s3removesanyuservariationfromsealapplicationensuringareproduciblesealtimeandtimeagainandenablingconfidenceintheconsistencyofsealingresults.
Thepressureappliedduringsealingisconstantandthetimeandtemperaturecanbeadjusted.Thiswillenablethesealofchoicetobeattainedreproducibly.Aneasilypeelablesealorastrongsealforstoragecanbeachievedwithoutfearofunderoroversealing.
Damagetomicroplatescausedbyoversealingispreventedenablingrepeatedresealingofplates.
The4s3iscompatiblewithanystandardSBSfootprintplateandcanalsobeusedtosealrackedtubes/tubestrips.
4titude®offersthelargestrangeofheatsealingmaterialsavailable,withpeelable,piercableandpermeablesealingfilmandfoils.
Forademonstrationortrialofa4s3pleasecontact4titudeoryourlocaldistributor.
set temperature
1
set time
2
press “operate”
4
load plate & seal
3
plate is sealed automatically
5
remove sealed plate
6
4s3The4s3isasemi-automaticheatsealercompatiblewithawiderangeofsealingmaterialsandplatesincludingPCR,qPCR,assayandstorageplates.
NEW
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Heat Sealing
Plate FormatsThe4s3iscompatiblewithalltypesofSBSfootprintplates:
• SBSstandardfootprintpolypropylene&polystyreneplates,useadapter4ti-0615
• SBSdeep-wellplates(96or384-well),useadapter4ti-0615
• 96wellPCRplates(skirted,semi-skirtedornon-skirted),useadapter4ti-0625
Adapters and Sealing AidsThe4s3employsauniqueandcost-savingadaptersystem.Deep-wellplatesandmostotherskirtedplatescanbesealedonthestandardplatecarrier(4ti-0615,suppliedwiththeunit).Forall96wellPCRplatesegmentsandtubestrips,anadditionalplatecarrier(4ti-0625,optionalextra)mustbeused.Bothplatecarrierscanbecombinedforeasierhandlingofshallowwellplates,suchas384-wellPCRplates.
Forimprovedsealingoffilmsorfoilsthathaveatendencytocurl,weoffersealingaids.Aweightedplaten(4ti-0602)forusewithdeep-wellplatesissuppliedwiththeunitandasealingframeforusewithallotherplatedesignsissuppliedwhenorderingtheoptional4ti-0625adapter.
Real Time DisplayTherapidheatingelementallowsforaquickstart-upwithinminutes.Thetemperatureisdisplayedinreal-timesotheheating-upprocesscanbemonitored.
Energy Saving FunctionsWhentheunitisleftidle,itautomaticallyswitchesintostand-bymode,duringwhichthetemperatureoftheheatingelementisreducedto60°C,tosaveenergy.Whenthesealerisleftidleforlongerperiods,itswitchesoffautomatically,foraddedsafety.Thetimesatwhichtheunitswitchesintostand-byandswitch-offmodescanbeadjustedtosuittheusers’needs.
RS-232 Serial Port for Automated IntegrationTheadditionofaserialportatthebackoftheunitmeansthe4s3canbeintegratedintoanexistingLIMSsystemwhereittrulybecomesanautomated,walk-awayheatsealer.
The4s3isSILAcompatibleandadriverforplugandplayuseinintegrationsystemsisavailable
4ti-0625: 96-well PCR plate adapter
4ti-0615: Standard plate adapter
4ti-0615 & 4ti-0625 interlocked for easy handling of shallow well plates
4ti-0616: Roche 1536-well plate adapter
4ti-0612: Sealing frame
4ti-0616 & 4ti-0625 interlocked for easy handling of Roche 1536-well plates
4ti-0602: Weighted sealing platen
Real-time temperature display
Technical Specification
Weight:9kg
Dimensions:310 x 275 x 181mm (HxDxW)
Voltage:110/230V
Power:300W
SealingTemp:60-200°C
SealingTime:0 - 10 seconds
Connection:RS-232 Serial port
4ti-0655 4s3HeatSealer
Includesuniversalpowersupply,powercord,manual,standardplate
adapter(4ti-0615)andweightedsealplaten(4ti-0602)
4ti-0615 Adapterfordeep-welland384-wellPCRplates
4ti-0616 AdapterforRoche1536-wellPCRplates
4ti-0602 Weightedsealplatentoholddown
sealingsheetswithatendencytocurl
4ti-0625 Adapterfor96-wellPCRplates,includessealingframe(4ti-0612)
4ti-0612 Sealingframeforusewith4ti-0625adapter
Ordering Information
Sealing Accessories and Tools
Code Description
www.4ti.co.uk | 65 +44 (0)1306 884885
Hea
t Se
alin
g
PEELABLE
A
UTOCLAVABLE
121o
ClearsealisanopticallyclearfilmwhichformsaneasilypeelableyetPCR/qPCRtightseal.Itsopticalclarityallowsuseforallopticalapplications.Theclearsealcanbeusedtosealmostplatetypesincludingpolypropylene,polyethylene,polystyreneandCOCplates.Availableassheetsoronarollforautomatedsealers.
Clear Seal
Applications
• qPCRorotheropticalapplications(e.g.fluorescenceorcolorimetricassays)
• Shorttermcompoundstorage
• Suitableformostplatematerials
• Availableinsheetorrollformatforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
Clearweldsealisanopticallyclearpolymerfilmwhichformsapermanentbondtopolypropyleneplatesthatcannotbepeeledandisdifficulttopierce.Thesealhasagoodsolventresistanceforsafelongtermstorage.
Samplecanbeaccessedifrequiredusingabladeorneedle.
NowalsoavailableasFrameSealproductseepage75
Clear Weld Seal Mark II
Applications
• PCR
• waterbaththermalcyclers
• qPCRandotheropticalapplications(e.g.fluorescenceandcolorimetricassays)
• Storageanddisposalofhazardousmaterials
• RecommendedforusewithDMSO
• Availableassheetsorinrollformatforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
*(110Cwithpressurisedheatedlid)
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-80°
80°
*
*Onceautoclaved,notrecommendedforPCRapplications
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-80°
110°
*
Code Pack Size/Dimensions
4ti-0573 610mx78mmroll1Approx5000seals
4ti-0573S Trialroll15mx78mm
4ti-0574 500mx115mmroll2Approx6250seals
4ti-0574S Trialroll25mx115mm
4ti-0575 100sheets(125mmx78mm)
4ti-0575/FS SealingFrame:50seals/frameperpack
Code Pack Size/Dimensions
4ti-0540 500mx78mmroll1Approx4200seals
4ti-0540/REMP 500mx78mmroll3Approx4200seals
4ti-0540S Trialroll15mx78mm
4ti-0542 350mx115mmroll2Approx4400seals
4ti-0542/REMP 500mx115mmroll4Approx6250seals
4ti-0542S Trialroll
4ti-0541 100sheets(125mmx78mm)5mx115mm
NEW
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Heat Sealing
TheClearSeal3730isaclearpolyesterbackedfilmwhichisparticularlythin.Itsealstopolypropylene,polystyrene,polyethyleneandCOCmicroplates.Thissealhasgoodopticalclaritywithmoderatesolventresistance.Thethinnatureofthissealenablesittobeeasilypiercedwithanauto-samplerneedle,ortheprobefromtheABi3730sequencer.
Thesealisonlyavailableinrollformat,butarollwithperforatedsheetscanbeusedforlowthroughput.
Clear Seal 3730
Applications
• PCR
• qPCRandotheropticalapplications(e.g.fluorescenceandcolorimetricassays)
• Piercablebyautosamplesneedles
• PiercablebytheABI3730
• Availableinrollformatforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
PIERCEABLE
ThispopularlaminatematerialgivesapeelableheatsealtoPPandCOCplates.Itcanbepeeleddirectlyfromtheplatefollowingremovalfromthefreezerandisagoodbarriertoaqueoussolutions.Thesealshowsmoderateresistancetosolventsatroomtemperatureandformsaweld-sealtopolyethyleneplates(cannotbepeeled).
Peel Seal
Applications
• Verylowtemperaturecompoundstorage.
• Mid-term(<5days)roomtemperaturecompoundstorage
• PCR
• Availableassheetsorinrollformatforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
1 Compatible with Thermo Fisher ALPS 300™ / ALPS 3000™ /
KBiosystems Wasp™ / KBioscience FlexiSeal and Cube2 Compatible with Agilent (Velocity 11) PlateLoc®
3 Compatible with REMP Portrait Heat Sealer (PHS)4 Compatible with REMP Landscape / Stacking Heat
Sealers (LHS/SHS)
COC = Cyclic Olefin Copolymer
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
80°
*(110C with pressurised heated lid)
*
*(110CwithpressurisedPCRheatedlid)
A
UTOCLAVABLE
121o
PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-200°
90°
*
Code Pack Size/Dimensions
4ti-0580 610mx78mmroll1Approx5000seals
4ti-0580S Trialroll15mx78mm
4ti-0582 500mx115mmroll2Approx6250seals
4ti-0582S Trialroll25mx115mm
4ti-0581 Rollof1000perforatedsheets125mmx78mm
Code Pack Size/Dimensions
4ti-0520 610mx78mmroll1Approx5000seals
4ti-0520S Trialroll15mx78mm
4ti-0522 500mx115mmroll2Approx6250seals
4ti-0522S Trialroll25mx115mm
4ti-0521 100sheets(125mmx78mm)
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Hea
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Pierce Seal
PEELABLE
ThislaminatematerialgivesapeelableheatsealtopolypropyleneandCOCplates.Itcanbepeeleddirectlyfromtheplateimmediatelyafterremovalfromthefreezer.TheDMSOresistantpeelsealisanexcellentbarriertoaqueoussolutionsandsolvents,inparticularDMSO,evenatroomtemperature.
Itformsaweld-sealtopolyethyleneplates(cannotbepeeled),requiringabladeorneedleforaccess.
DMSO Resistant Peel Seal
Applications
• LowandroomtemperaturecompoundstoragewithDMSO*andotherorganicsolvents
• 100%DMSOcanbestoredatroomtemperaturefor12monthswithnodeteriorationofthesealquality
• Availableinsheetandrollformatforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
Thisfoil-basedheatsealissuitablefortopolypropyleneandpolystyreneplatesandcanbeeasilypiercedwithamanualorautopipettetip.Ithasverygoodsolventresistance,includingtoDMSO.Sealscanbeappliedontopofeachotherallowingmultipleaccess/resealingofsamples.Abluecolourprintidentifiesthenon-sealingsurfaceforeaseofapplication.Thissealremainsintacttoveryhightemperatures.
Applications
• PCR
• LowandroomtemperaturecompoundstoragewithDMSOandotherorganicsolvents
• Sampleshipping
• Availableinsheetandrollformatforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-80°
40°
A
UTOCLAVABLE
121o
PIERCEABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
120°
Code Pack Size/Dimensions
4ti-0585 500mx78mmroll1Approx4200seals
4ti-0585/REMP 500mx78mmroll3Approx4200seals
4ti-0585S Trialroll15mx78mm
4ti-0586 500mx115mmroll2Approx6200seals
4ti-0586/REMP 500mx115mmroll4Approx6200seals
4ti-0586S Trialroll25mx115mm
4ti-0587 100sheets(125mmx78mm)
Code Pack Size/Dimensions
4ti-0530 610mx78mmroll1Approx5000seals
4ti-0530/REMP 610mx78mmroll3Approx5000seals
4ti-0530S Trialroll1
4ti-0532 500mx115mmroll2Approx6200seals
4ti-0532/REMP 500mx115mmroll4Approx6200seals
4ti-0532S Trialroll2
4ti-0531 100sheets(137mmx76mm)
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Heat Sealing
TheINDISealisafoil-basedheatsealthatsealstopolypropyleneandissuitableforlowtemperaturestorage.Thesealconsistsof96perforatedindividualsealingcapsinafoilframe.Theframeisremovedafterheatsealingleavingeachwellwithanindividualairtightseal.Thesealshavetabsforeasyremovalbyhandorwithforceps,withoutleavinganyresidue.
Mainlydevelopedforusewithtubesinclusterrackssomeplatescanalsobesealed.
Re-sealingandpiercingarepossible.
96 INDI™ Seal
Applications
• Compoundstorage
• Sampleshipping
• FreefromDNase,RNaseandhumangenomicDNA
AnaluminiumheatsealthatsealstoPPandPSplatesandcanbepiercedwithapipettetiporpeeledbyhand.GoodsolventresistanceincludingDMSO.Resealingontoanexistingsealispossible.Aredcolourprintidentifiesthenon-sealingsurfaceforeaseofapplication.
Foil Seal
Applications
• Lowtemperaturecompoundstorage
• Short-termroomtemperaturecompoundstorage
• PCR
• Resealable
• Availableinsheetandrollformatforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
PEELABLE
A
UTOCLAVABLE
121o
PIERCEABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
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E
-0°
110°
PIERCEABLEPEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-195°
120°
Code Pack Size/Dimensions
4ti-0543 INDISealforindividualtubesealing
(125mmx78mmsheets) 50sheets
Code Pack Size/Dimensions
4ti-0535 610mx78mmroll1Approx5000seals
4ti-0535/REMP 610mx78mmroll3Approx5000seals
4ti-0535S Trialroll15mx78mm
4ti-0537 500mx115mmroll2Approx6200seals
4ti-0537/REMP 610mx115mmroll4Approx6200seals
4ti-0537S Trialroll25mx115mm
4ti-0536 100sheets(125mmx78mm)1 Compatible with Thermo Fisher ALPS 300™ / ALPS 3000™ /
KBiosystems Wasp™ / KBioscience FlexiSeal and Cube2 Compatible with Agilent (Velocity 11) PlateLoc®
3 Compatible with REMP Portrait Heat Sealer (PHS)4 Compatible with REMP Landscape / Stacking Heat
Sealers (LHS/SHS)
COC = Cyclic Olefin Copolymer
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Hea
t Se
alin
g
Thisisaheavydutylaminatematerialthatsealstopolypropylenetogiveaverystrongbutpeelableseal.Thissealaffordsahighdegreeofsampleprotection.GoodsolventresistanceincludingDMSO.
Thermal Bond
Applications
• Longtermstorage,includingstorageandtransportationatlowtemperature
• Storageoforganicsolvents,acidsandalkalinesincludingDMSO
• PCR
• Suitableforwaterbaththermalcyclers
• Availableinsheetandrollformatsforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
A
UTOCLAVABLE
121o
PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
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E
-200°
110°
Thepolystyrenefoilsealcreatesastrong,butpeelablesealtopolystyreneplates.Thesealcanalsobepiercedwithapipettetipandhasagoodsolventresistance,includingtoDMSO.Resealingontoanexistingsealispossible.
Polystyrene Foil Seal
Applications
• Lowtemperaturecompoundstorage
• Short-termroomtemperaturecompoundstorage
• Resealingispossible
• FreefromDNase,RNaseandhumangenomicDNA
Code Pack Size/Dimensions
4ti-0547 100sheets(125mmx78mm)
PIERCEABLEPEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
110°
Code Pack Size/Dimensions
4ti-0590 500mx78mmroll1Approx4200seals
4ti-0590S Trialroll15mx78mm
4ti-0592 300mx115mmroll2Approx3700seals
4ti-0591 100sheets(125mmx78mm)5mx78mm
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Heat Sealing
Thisisafoil-basedheatsealwhichiscoatedonthesealingsurfacewithalowautofluorescenceblackpigmentforusewithlightsensitivesamplesorforfluorescentassays.TheBlackSealformsapeelablesealtopolystyreneplates.
Black Seal
Applications
• Fluorescentandotherlightsensitiveassays.
• Pierceswithaforcegreaterthan10N(ASTMD1000)
• Availableinsheetandrollformatsforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
1 Compatible with Thermo Fisher ALPS 300™ / ALPS 3000™
/ KBiosystems Wasp™ / KBioscience FlexiSeal and Cube2 Compatible with Agilent (Velocity 11) PlateLoc®
COC = Cyclic Olefin Copolymer
Code Pack Size/Dimensions
4ti-0525 610mx78mmroll1Approx5000seals
4ti-0526 500mx115mmroll2Approx6200seals
4ti-0527 100sheets(125mmx78mm
PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
80°
PIERCEABLE
ThisheatsealhasasmallerporesizethanthediscontiunedGasPermeableSeal(4ti-0596)whichstillallowsforgaseousexchange,butlimitsevaporation.Handlinginautomatedsealersisimproved.TheGasPermeableHeatSealMk2issuitableforcellcultureandisavailableassterileproductforthisapplication.
Gas Permeable Seal Mk 2
Applications
• Cellculture
• Overnightincubation
• Seedandinsectstorage
• Permeabilityrateis>20g/m2/24h
• Poresizeis<20µm
• Availableinsheetandrollformatforautomatedheatsealers
• FreefromDNase,RNaseandhumangenomicDNA
A
UTOCLAVABLE121o
PEELABLE
PIERCEABLESTERILE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
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E-20°
80°
Code Pack Size/Dimensions
4ti-0598 GasPermeableSealMk2roll(610mx78mm)1Approx5000seals 1roll
4ti-0598/ST GasPermeableSealMk2roll,sterile(610mx78mm)1
Approx5000seals 1roll
4ti-0599 GasPermeableSealMk2roll(500mx115mm)2Approx6200seals 1roll
4ti-0599/ST GasPermeableSealMk2roll,sterile(500mx115mm)2
Approx6200seals 1roll
4ti-0597 GasPermeableSealMk2sheets(125mmx78mm) 100sheets
4ti-0597/ST GasPermeableSealMk2sheets,sterile(125mmx78mm) 100sheets
(10packsx10sheets)
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Hea
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Gas Permeable Clear Seal
Applications
• Insectstudies
• Seedstorage
• Availableinsheetandrollformatforautomatedsealers
• FreefromDNase,RNaseandhumangenomicDNA
PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
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E
-80°
110°
Thisisaclearpolymerfilmformingapeelablesealtopolypropylene,polyethylene,polystyreneandCOCplateswithgoodopticalclarityandmoderatesolventresistance.Thefilmhas3mmslitsacrosstheentirewidthoftherollorsheet,allowinggaseousexchange.DuetoitswidertemperaturetolerancecomparedtotheGasPermeableHeatSealitcanbeusedforstoragee.g.forseedsorinsects.
Code Pack Size/Dimensions
4ti-0540/SLIT 480mx78mmroll1Approx4000seals
4ti-0541/SLIT 100sheets(125mmx78mm)
1 Compatible with Thermo Fisher ALPS 300™ / ALPS 3000™
/ KBiosystems Wasp™ / KBioscience FlexiSeal and Cube2 Compatible with Agilent (Velocity 11) PlateLoc®
COC = Cyclic Olefin Copolymer
| www.4ti.co.uk72 +44 (0)1306 884885
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po
un
dsto
rage,
sho
rttermro
om
temp
erature
com
po
un
dsto
rage
(<5d
ays),PC
R.
Lo
wtem
peratu
re/
roo
mtem
peratu
re
com
po
un
dsto
rage
with
DM
SO
&o
ther
org
anicso
lvents.
PC
R,co
mp
ou
nd
storag
e,samp
le
ship
pin
g
Lowtem
perature
compound
storage,short-term
room
temperature
compoundstorage,
PC
R.
Lowtem
perature
transportation&
storage.PC
R,esp.
waterbathcyclers.
Storageoforganicsolvents,acids
&alkalines.
Flu
orescen
t&
oth
erligh
tsen
sitiveassays
Cellcu
lture,o
vern
igh
tincu
batio
n,
seedan
din
sectsto
rage
Sto
ragee.g
.fo
rseedso
rin
sects
Co
mp
ou
nd
sto
rae,sam
ple
ship
pin
g
Sp
ecialP
rop
ertiesG
oo
do
ptical
clarity.
Mo
derate
solven
tresistan
ce.
Go
od
op
ticalclarity&
resistantto
DM
SO
.
Go
od
op
ticalclarity,
som
esolven
t
resistance.
Can
bep
eeled
directlyfro
m-8
0°C
freezer.Mo
derate
resistanceto
solven
tsatroo
m
temp
erature.
Can
bep
eeled
directlyfro
m-8
0°C
freezer.Hig
h
resistanceto
solven
tseven
atelevated
temp
eratures.
Easilypierceable.
ResistanttoD
MS
O.
Re-sealablew
ith
anotherPierceS
eal.
Colourprint
identifiesnon-
sealingsurface
Re-sealab
lewith
ano
therFo
ilSeal.
Resistan
ttoD
MS
O.
Co
lou
rprin
tid
entifi
esno
n-
sealing
surface.(Fo
ilS
ealon
ly)
Verystro
ng
sealw
ithP
P.Resistan
tto
DM
SO
and
oth
erso
lvents
Afo
il-based
h
eatseal,co
atedo
nth
esealin
gsu
rfacew
ithalo
w
flu
orescen
ceb
lackpig
men
t
Sm
allporesizethatallow
sgaseousexchange,but
limitsevaporation.
Perm
eabilityrate>20
g/sqm/24
h
20µmporesize
3m
mslits
acrossen
tiresu
rfaceofseal
makesth
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ermeab
leto
gases
96
perfo
rated
ind
ividu
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gcap
sin
afoil
frame.C
an
beresealed
Min
Temp
-80
°C-8
0°C
-20
°C-8
0°C
-80
°C-2
0°C
-20
°C-2
00
°C-8
0°C
-20
°C-8
0°C
-195
°C
Max Tem
p8
0°C
(or110
°Cw
ith
pressurised
PC
Rheatedlid)
110°C
80
°C(o
r110
°Cw
ith
pressu
rised
PC
Rh
eatedlid
)
90
°C(o
r110°C
with
p
ressurised
PC
R
heated
lid)
40
°C12
0°C
110°C
110°C
120
°C8
0°C
100
°C12
0°C
Piercab
le•
••
••
•
Peelab
le•
••
••
••
••
RN
ase /DN
asefree
••
••
••
••
••
•
Material
Lam
inate
Lam
inate
Po
lymer
Lam
inate
Lam
inate
Foil
Foil
Lam
inate
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Wo
venm
aterialL
amin
ateL
amin
ate
Sealsto
PP,P
E,P
S,
CO
CP
PP
P,PE
,PS
,C
OC
PP,C
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PP,P
E
PP,P
SP
P,PS
PP
PS
PP,P
S,C
OC
PP,P
E,P
S,C
OC
PP
4s3sealingtim
ew
ith96
wellP
P
Plates(°C
/secs)^
175-18
5/2-3
175-18
5/2-3
165
-175/3
175-18
5/3
175-18
5/3
160
-175/2
165
-180
/2170
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/2-3-
170/2
175-18
5/2-3
175/3
4s3sealingtim
ew
ith384
wellP
P
Plates(°C
/secs)^
165
-180
/3170
-175/2-3
165
-175/2
170-175
/2-3170
-175/2-3
160
-175/2
165
-175/2-3
160
-170/2
-170
/216
5-18
0/3
-
4s3sealingtim
ew
ithVisionP
lates(°C
/secs)^
185
-20
0/3
-175
-185
/2-3-
-18
5-2
00
/318
5-2
00
/3-
185
-20
0/3
170/2
185
-20
0/3
-
Pro
du
ct Co
de
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Ro
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4ti-0
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ti-05
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ti-05
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4ti-0
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ti-05
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4ti-0
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ti-05
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ti-05
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4ti-0
52
54
ti-05
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4ti-0
54
0/S
LIT
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Ro
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4ti-0
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0/
RE
MP
4ti-0
573/R
EM
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ti-05
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EM
P4
ti-05
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MP
4ti-0
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5/R
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P4
ti-05
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/RE
MP
4ti-0
535
/RE
MP
4ti-0
59
0/R
EM
P4
ti-05
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ti-05
98
/RE
MP
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Ro
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4ti-0
54
24
ti-05
744
ti-05
82
4ti-0
52
24
ti-05
86
4ti-0
532
4ti-0
537
4ti-0
59
24
ti-05
264
ti-05
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4ti-0
54
2/S
LIT
-
Ro
ll115m
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4ti-0
54
2/
RE
MP
4ti-0
574
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4ti-0
58
2/
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4ti-0
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2/R
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ti-05
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4ti-0
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MP
4ti-0
537/R
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P4
ti-05
92
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MP
4ti-0
526
/R
EM
P4
ti-05
99/R
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-
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ti-05
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4ti-0
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54
ti-05
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4ti-0
52
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ti-05
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ti-05
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4ti-0
53
64
ti-05
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4ti-0
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ti-05
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4ti-0
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IT4
ti-05
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qu
ire.
www.4ti.co.uk | 73 +44 (0)1306 884885
Hea
t Se
al S
elec
tion
Tab
le
Thistablemakesitsimplerforyoutoselecttherightheatsealforyoursealer:
Choosewhichtypeofsealyourequireacrossthetopandfollowdowntothecorrectrowforyourheatsealer,listedontheleft.Takeanoteoftheproductcode.
Cle
ar
Se
al
p6
6
Cle
ar
We
ld S
ea
l M
ark
II
p6
6
Cle
ar
Se
al
37
30
p6
7
Pe
el
Se
al
p6
7
DM
SO
R
esi
sta
nt
Pe
el
Se
al
p6
8
Pie
rce
S
ea
l
p6
8
Foil
Se
al
p6
9
PS
Fo
il S
ea
l
p7
0
Th
erm
al
Bo
nd
p7
0
Bla
ck
Se
al
p7
1
Ga
s P
erm
ea
ble
S
ea
l M
k 2
p7
1
Ga
s P
erm
ea
ble
C
lea
r S
ea
l
p7
2
IND
I S
ea
l
p6
9
4ti
tud
e
4s3
4ti
-05
41
4ti
-05
754
ti-0
58
14
ti-0
52
14
ti-0
58
74
ti-0
531
4ti
-05
36
4ti
-05
47
4ti
-059
14
ti-0
52
74
ti-0
597
4ti
-05
41/
SL
IT4
ti-0
54
3
A4
S4
ti-0
54
04
ti-0
573
4ti
-05
80
4ti
-05
20
4ti
-05
85
4ti
-05
30
4ti
-05
354
ti-0
54
54
ti-0
59
04
ti-0
52
54
ti-0
59
84
ti-0
54
0/S
LIT
-
Ag
ilen
t
Pla
teL
oc
4ti
-05
42
4ti
-05
744
ti-0
58
24
ti-0
52
24
ti-0
58
64
ti-0
532
4ti
-05
37-
4ti
-05
92
4ti
-05
264
ti-0
59
9-
-
KB
iosc
ien
ce
Fle
xiS
eal
4ti
-05
40
4ti
-05
734
ti-0
58
04
ti-0
520
4ti
-05
85
4ti
-05
30
4ti
-05
35-
4ti
-05
90
4ti
-05
254
ti-0
59
84
ti-0
54
0/S
LIT
-
KB
iosy
ste
ms
E-F
ly2
4ti
-05
41
4ti
-05
754
ti-0
58
14
ti-0
52
14
ti-0
58
74
ti-0
53
14
ti-0
53
64
ti-0
54
74
ti-0
59
14
ti-0
52
74
ti-0
59
74
ti-0
54
1/S
LIT
4ti
-05
43
Was
p4
ti-0
54
04
ti-0
573
4ti
-05
80
4ti
-05
20
4ti
-05
85
4ti
-05
30
4ti
-05
35-
4ti
-05
90
4ti
-05
25
4ti
-05
98
4ti
-05
40
/SL
IT-
Cha
mel
eon™
4ti
-05
40
4ti
-05
734
ti-0
58
04
ti-0
52
04
ti-0
58
54
ti-0
53
04
ti-0
535
-4
ti-0
59
04
ti-0
52
54
ti-0
59
84
ti-0
54
0/S
LIT
-
RE
MP
Easy
Sea
ler
4ti
-05
41
4ti
-05
754
ti-0
58
14
ti-0
52
14
ti-0
58
74
ti-0
53
14
ti-0
53
64
ti-0
54
74
ti-0
59
14
ti-0
52
74
ti-0
59
74
ti-0
54
1/S
LIT
4ti
-05
43
Po
rtra
it4
ti-0
54
04
ti-0
573
4ti
-05
80
4ti
-05
20
4ti
-05
85
4ti
-05
30
4ti
-05
35-
4ti
-05
90
4ti
-05
25
4ti
-05
98
4ti
-05
40
/SL
IT-
Th
erm
o F
ish
er
AL
PS
30
0™
4ti
-05
40
4ti
-05
734
ti-0
58
04
ti-0
52
04
ti-0
58
54
ti-0
53
04
ti-0
535
-4
ti-0
59
04
ti-0
52
54
ti-0
59
84
ti-0
54
0/S
LIT
-
AL
PS
30
00
™4
ti-0
54
04
ti-0
573
4ti
-05
80
4ti
-05
20
4ti
-05
85
4ti
-05
30
4ti
-05
35-
4ti
-05
90
4ti
-05
25
4ti
-05
98
4ti
-05
40
/SL
IT-
AL
PS
™5
04
ti-0
54
14
ti-0
575
4ti
-05
81
4ti
-05
21
4ti
-05
87
4ti
-05
31
4ti
-05
36
4ti
-05
47
4ti
-05
91
4ti
-05
27
4ti
-05
97
4ti
-05
41/
SL
IT4
ti-0
54
3
ALP
S™2
54
ti-0
54
14
ti-0
575
4ti
-05
81
4ti
-05
21
4ti
-05
87
4ti
-05
31
4ti
-05
36
4ti
-05
47
4ti
-05
91
4ti
-05
27
4ti
-05
97
4ti
-05
41/
SL
IT4
ti-0
54
3
All
roll
seal
fo
rmat
sar
eav
aila
ble
ina
tri
alr
oll.
Ad
dS
to
th
een
do
fth
ep
rod
uct
co
de
too
rder
If
yo
uw
ou
ldli
kea
ssis
tan
cein
ch
oo
sin
gt
he
corr
ect
seal
fo
ryo
ur
app
licat
ion
an
dh
eat
seal
er,p
leas
eco
nta
ctt
ech
nic
als
up
po
rto
n+
44
(0
)13
06
88
48
85
or
info
@4
ti.c
o.u
k
Heat Seal Selection Table
| www.4ti.co.uk74 +44 (0)1306 884885
Code Description Qty
4ti-0575/FS ClearWeldSealMkIIFrameSealTM,stackableheatsealframes 50
4ti-0541/FS ClearSealFrameSealTM 50
4ti-0531/FS PierceSealFrameSealTM 50COMING SOON
FrameSealTMutilisesasheetofstandard4titude®heatsealmaterialmountedontoadisposable,rigid,plasticframe.AFrameSealTMcanbeplacedeithermanuallyorwitharoboticgripperontoawiderangeofskirtedandsemi-skirtedmicroplatesincludingPCRplatesandarecompatiblewiththe4S3heatsealer.Aftersealing,thereistheoptiontoremovetheframebytearingalongpre-cutlinesleavingtheheatsealinplaceontheplatesurface.
FrameSeal™
Application example:
OurClearWeldSealMkII(4ti-0575)heatsealingfilmisnowavailablepre-mountedtocreateaFrameSeal.TogetherwithFraunhofferIPAInstituteFrameSealTMhasbeenusedinafullyintegratedsolutionforsealing1536wellPCRplatesbeforeanalysisofRNAiscreeningwithaRocheLightcycler1536.
Usingaroboticarm,asingleFrameSealTMisplacedontoa1536wellPCRplate.Byadjustingthepositionoftherobotgripper,theplateandFrameSealTMcombinationarethentransferredtothedrawofa4S3HeatSealerwhichsealsthepartviaasoftwarecommandthroughitsRS232port.
Afterthesealingprocesshasbeencarriedout,thesealedplateistransferredtotheDe-Framer,whichseperatestheframefromthefilmateachpointalongtheedgeofthefilm.Theframecanthenberemovedleavingthesealedplatetobeplacedinthecycler.
TogethertheheatsealerandtheFrameSealTMpresentedhereare:
• Themostcosteffectiveautomatedheatsealingsolutionavailable
• Anapproachinlinewithdemandsofdiagnosticsforasterile,single-useconsumable
• Themostcompactinstrumentforintegration
4s3™shownnexttotheDe-Framer
Automationcell,showingthe4LAB™,4s3™andDe-Framerfullyintegrated
Key advantages
• Keyadvantagescomparedtosheetsofsealingfilmorrollmaterial,aretheeaseofhandlingandtheabilitytosteriliseandimprovethequalitycontrolofeverysingleseal.
• TheFrameSealTMalsoenablestheuseofsheetedsealingmaterialinautomation.
NEW
COMING SOON
www.4ti.co.uk | 75 +44 (0)1306 884885 www.4ti.co.uk | 75 +44 (0)1306 884885
AdhesiveSealing
Adhesivesealsprovideaversatile,yetrobust,methodforprotectingsamples.Thesealsaresuppliedassheetsandsomearealsoavailableinrollformat.Mostadhesivesealsareprovidedwithendtabsforeasyapplicationandremoval.
AlloursealsareproducedandprocessedunderstrictlycontrolledenvironmentalconditionsandcertifiedfreefromDNase,RNaseandhumangenomicDNA.
Tochoosethemostsuitablesealpleaserefertocomparisontablepage83whichdescribestherecommendedapplicationsandtechnicalfeaturesofeachseal.
Toobtainthebestsealingresultswithadhesivesealswestronglyrecommendedtheuseofhighqualityplateslikeourswithraisedrimsealingringsforoptimalsealingintegrityandguaranteedflatness.Toimprovesealapplicationweoffersupplementaryproductslikethesealrollerpage53andthecompressionpadseepage52.
WehaveaddedthreenewsealstoourrangeformorespecialisedapplicationsincludinggaspermeablesealsandanInterSealTMideallysuitedforkitmanufacturers,p82.
4titude®offerawiderangeofsealsfromgeneralmicroplatecoverstospecialisedgaspermeablesealsforcellcultureapplications.
| www.4ti.co.uk76 +44 (0)1306 884885 | www.4ti.co.uk76 +44 (0)1306 884885
Adhesive Sealing
Applications
• PCR
• qPCRandotheropticalapplications
• lowtemperaturestorageandtransportation
• availableinsheetandrollformat
• FreefromDNase,RNaseandhumangenomicDNA
PCR Seal
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
PEELABLE
Applications
• SuitableforqPCRandotheropticalapplications
• Forusewith96-wellplatesonly
• FreefromDNase,RNaseandhumangenomicDNA
Q-Stick™ qPCR Seal
Code Pack Size/Dimensions
4ti-0565 100sheets(133mmx76mm)
PEELABLE
ThePCRsealisadurabletransparentpolyesterfilmwithastrongadhesivelayer.Thissealenablesahighsealintegrityandefficientlypreventssampleevaporation.ItisrecommendedforPCR,qPCRandotheropticalapplications(e.g.fluorescenceorcolorimetricmeasurements)aswellassamplestorage.
Adhesivefreeendtabsfacilitateapplicationandremoval.Thesealscanbeeasilypeeledfromtheplatewithoutleavinganystickyresidue.
* (with pressurised heated PCR lid)
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
110°
*
Code Pack Size/Dimensions
4ti-0500 100 sheets (135mm x 80mm)
4ti-0501 1 laminated roll (100m x 80mm) Approx 700 seals
ThisuniquesealcombinesthestrongsealingintegrityofourPCRsealwithimprovedopticalpropertiesthanksto96adhesive-freewindows.ItisrecommendedforqPCRandotheropticalapplications(e.g.fluorescenceorcolorimetricmeasurements).
Theuseofadhesivefreeendtabsenableseasyalignmentofthewindowsona96wellplateaswellaseasyremovaloftheseal.
* (with pressurised heated PCR lid)
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
110°
*
www.4ti.co.uk | 77 +44 (0)1306 884885
Adh
esiv
e Se
alin
g
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
• Noadhesivetocomeintocontactwithsamples
• EnhancedopticalclaritysuitableforqPCR
• Suitableforallplatetypes
• Peelablewithoutleavingstickyresidueontheplatewhenthesealispeeledoff
• SuitableforqPCRandotheropticalapplications
• Availableinsheetandrollformat
• FreefromDNase,RNaseandhumangenomicDNA
qPCR Seal
Thisopticallyclearsealwasspecificallydevelopedforopticalapplications,particularilyqPCR.Itisnon-tackytoskinandglovesenablingeaseofhandlingandapplicationandpositioning.Thissealisapressureactivatedseal.Thepolyesterfilmhasadhesiveheldwithincapsulesontheunderside,andpressuremustbeappliedtothetop-side,aroundtheraised-wellrimstoactivatetheadhesive.
PEELABLE
Applications
• PCRandotherhightemperatureapplications
• Samplestorage
• Suitableforallplatetypes
• Availableinsheetandrollformat
• FreefromDNase,RNaseandhumangenomicDNA
PCR Foil Seal
PIERCEABLE PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-40°
120°
*(with pressurised heated PCR lid)
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-40°
110°
*
Code Pack Size/Dimensions
4ti-0560 100sheets(140mmx77mm)
4ti-0561 1roll(100mx80mm)Approx700seals
Thisaluminiumfoilsealhadastrongacrylicadhesivewhichproducesasealofhighintegrity.ThissealwasdevelopedforPCRandotherhightemperatureapplicationsduetoitseffectivenessinpreventingsampleevaporation.ThePCRFoilSealisapiercableseal.Whenpierced,theirregularmannerinwhichthefoiltearspreventstheformationofavacuum.
Thesealhasperforatedendtabsforeasyapplicationandremovalbypeeling.
Code Pack Size/Dimensions
4ti-0550 100sheets(130mmx80mm)
4ti-0551 1roll(200mx80mm)Approx1400seals
| www.4ti.co.uk78 +44 (0)1306 884885
Adhesive SealingApplications
• Suitablefor96wellplates(autosamplers,HPLC,DNAsequencers)
• Piercableforsampleretrieval
• Availableinsheetandrollformat
• Endtabsforeaseofapplicationandremoval
• FreefromDNase,RNaseandhumangenomicDNA
Pierceable Film*
Code Pack Size/Dimensions
4ti-0566 100sheets(135mmx77mm)
4ti-0567 1roll(100mx80mm)Approx700seals
PIERCEABLE PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
110°
Applications
• Eukaryoticcellculture,bacterialcultureandovernightincubations.
• MoistureVapourTransmissionRateis6200g/m2/day
• AirPorosityis15sec/100cc/in2
• Suitableforallplatetypes
• FreefromDNase,RNaseandhumangenomicDNA
Gas Permeable Seal
STERILE
PIERCEABLE PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
80°
Thisadhesivesealisperforatedwithmicroporessothatitispermeabletogasesbutprovidesabarriertobacteriaandothersolidcontaminants.Thegaspermeablesealisaconvenientoptionforcoveringcellculturesandcanbepiercedorpeeledwithoutanyremainingstickyresidue.
Endtabsfacilitateeaseofapplicationandremoval.
Thisstrongadhesivesealwasdevelopedtofacilitateeasysampleremovalwithamanualorautomatedsystem.Thesealisatransparentpolyesterfilmwithastrongadhesivelayer.96adhesivefreewindowshavebeencross-cut.Thisenablesaccesstothesamplewellswithatiporprobewithminimalpressure.
SampleevaporationisminimizedwhenautosamplerssuchasHPLCandcapillarysequencersareused.
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
Code Pack Size/Dimensions
4ti-0515 50sheets(135mmx80mm)
4ti-0515/ST 50sterilesheets(5packsof10sheets,135mmx80mm)
4ti-0515/R 1roll(100mx80mm)Approx700seals
*NotavailableintheUSA
www.4ti.co.uk | 79 +44 (0)1306 884885
Adh
esiv
e Se
alin
g
*NotavailableintheUSA
• Suitableforeukaryoticcellculture,bacterialcultureandlong-termliveassays
• Suitablefor96or384wellplates
• MoistureVapourTransmissionRateis1g/m2.d.bar
• AirPorosityis600cm3/m2.d.bar
• Peelableleavingnoresidue
• Endtabsforeaseofapplicationandremoval
• FreefromDNase,RNaseandhumangenomicDNA
Gas Permeable Moisture Barrier Seal
PEELABLE
STERILEPIERCEABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
80°
Applications
• Suitableforbacterialorcellculture.
• MoistureVapourTransmissionRateis4200g/m2/day
• AirPorosityis10sec/100cc/in2
• Strongsolventresistance
• Availableinsheetorrollformat
• FreefromDNase,RNaseandhumangenomicDNA
Air-O-Seal
Air-O-Sealisahydrophobic,gaspermeable,adhesivesealwhichismadefromtapewithanacrylateadhesive.Air-O-SealhasamuchlowermoisturetransferratethanourstandardGasPermeableAdhesiveSeal(4ti-0515).Itisresistanttoacids,DMSO,detergents,ethanol,glycerol,ispropanol,sodiumhypochlorite(bleach)andsuitableforbacterialorcellculture.
Code Pack Size/Dimensions
4ti-0517 100sheets(135mmx80mm)
4ti-0517S 100sterilesheets(10packsof10sheets,135mmx80mm)
4ti-0517R 1roll(150mx80mm)Approx1250seals
STERILEPEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
30°
Thisisastrongadhesivesealthathas96or384adhesivefreewindows.ThesewindowsallowairandCO2exchange,butpreventmoistureevaporationfromthesample.
Thesealisprimarilyusedincellcultureforeukaryoticorbacterialcells.Itisultraclearforimagingwithouthavingtoremovetheseal.
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
Code Pack Size/Dimensions
4ti-0516/96 50sheets(5packsof10sheets,137mmx77mm),96round-wellonly
4ti-0516/384 50sheets(5packsof10sheets,137mmx77mm),384square-wellonly
NEW
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Adhesive SealingApplications
• Suitableformediumtolong-term(<5yrs)storageofsamplescontainingahighconcentration(<80%)ofDMSO.
• Strongadhesiveseal
• Suitableforallplatetypes
• FreefromDNase,RNaseandhumangenomicDNA
Peelable Solvent Resistant Foil
Code Pack Size/Dimensions
4ti-0512 100sheet(122mmx80mm)
PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
80°
Applications
• Generalmicroplatecover
• Reducesshorttermsampleevaporation
• Eliminateriskofcontamination(Aqueoussamplesonly)
• Suitableforallplatetypes
• Availableinsheetandrollformats
• FreefromDNase,RNaseandhumangenomicDNA
Microplate Seal
PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
80°
Thissealisbasedonanaluminiumfoilwithafilmlayerandsiliconeadhesive.Thislaminatehasaveryhighlevelofsolventresistanceandisrecommendedforlongtermsamplestorage.
Asingleendtabaidsapplicationandremovalbypeeling.
Thistransparentpolyesterbasedfilmhasamediumstrengthadhesive.Itisdesignedasalowcostsealingoptionandusefulfortemporarystorageandasacoverforapplicationssuchascentrifugation.
Endtabsallowforeasyapplicationandremoval.Thissealisremovablewithoutresidueontheplate.
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
For best results
For all adhesive seals, the best
sealing results are achieved using the
adhesive seal roller (4ti-0502).
Code Pack Size/Dimensions
4ti-0510 100sheet(130mmx80mm)
4ti-510/R 1roll(110mx80mm)Approx800seals
www.4ti.co.uk | 81 +44 (0)1306 884885
Adh
esiv
e Se
alin
g
Applications
• Bacterialculture
• Cellculture
• Moisturevapourtransmissionrateof450gms/m2/day
• FreefromDNase,RNaseandhumangenomicDNA
• Suitableforallplatetypes
Applications
• Idealforkitmanufacturerstoallowflexibleaccesstoindividualwells
• ForusewithVariStrips(inaframe)orBreak-A-Wayplates
• Re-sealingwithouttheneedforaheatsealer
• Endtabsforeasyapplicationandremoval
Double Skin Breathable Film
InterSeal
Code Pack Size/Dimensions
4ti-0518 100sheets(135x83mm)
Code Pack Size/Dimensions
4ti-0519 100sheets(148mmx98mmincludingliner)
PEELABLE
PIERCEABLE PEELABLE
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
0°
40°
SE
AL
INT
EG
R
ITY TEMPERATU
RE
RA
NG
E
-20°
110°
Thissealisanultrathinopaquemembranewhichisgaspermeablewhilstprovidinganeffectivebarriertomoisture.AirandCO2exchangeispossible,butevaporationisprevented.
Endtabsaidapplicationofthissealanditsremovalbypeeling.
Adoublesidedblackadhesivemicroplatefilmtofacilitatethesealing,accessing(piercing)andresealingofVariStrips(4ti-0753)priortoPCR.
Thestripsorplatesarefilledwithreagentandsealedwith4ti-0530(PierceSeal).TheIntersealisoverlaidandthestripsorplatesprocessedwiththePlateCutter4ti-3000intoindividuallysealedstrips.ThestripscanthenbetransportedandopenedatacustomersitebypeelingtheprotectedlineroftheInterSealoffandaccessingthesamplethroughthepierceseal.Thestripscanthenbesealedwithafoilorfilmwhichisappliedtotheexposedblackadhesivewithouttheneedforaheatsealer.
Also see
Variplates tm (page 42-43)
plate cutter (page 53)
NEW
NEW
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Adhesive Seal C
omparison Table
PC
R S
ea
l
p7
7
Q-S
tick™ fo
r q
PC
R
p7
7
qP
CR
Se
al
p78
PC
R Fo
il Se
al
p78
Pie
rcab
le F
ilm
p7
9
Ga
s Pe
rme
ab
le
Se
al
p7
9
Ga
s Pe
rme
ab
le
Mo
isture
B
arrie
r Se
al
p8
0
Air-O
-Se
al
p8
0
Pe
ela
ble
S
olve
nt
Re
sistan
t Foil
p8
1
Micro
pla
te S
ea
l
p8
1
Do
ub
le S
kin
Bre
ath
ab
le F
ilm
p8
2
Inte
rSe
al
p8
2
Ap
plica
tion
PC
Rq
PC
R,
fluo
resce
nce.
96
-we
ll m
icrop
late
s o
nly
qP
CR
& o
the
r flu
ore
scen
t a
pp
licatio
ns
PC
R &
sam
ple
sto
rag
eS
am
ple
a
pp
licatio
n
or re
trieval.
96
-we
ll pla
tes
on
ly.
Ce
ll & b
acte
rial
cultu
reE
uka
ryotic
cell cu
lture,
ba
cteria
l
cultu
re a
nd
lo
ng
-term
live
assays.
Ba
cteria
l or ce
ll cu
lture
Micro
pla
te
sea
ling
co
nta
inin
g
solve
nts in
cl. D
MS
O
Aq
ue
ou
s sa
mp
le sto
rag
eB
acte
ria cu
lture
Dia
gn
ostic kit
pro
du
ction
Sp
ecia
l Pro
pe
rties
Go
od
op
tical
clarity
Discre
et o
ptica
l w
ind
ow
s for 9
6-
we
ll pla
tes
Go
od
op
tical
clarity
Irreg
ula
r te
arin
g w
he
n
pie
rced
p
reven
ts fo
rma
tion
of
vacu
um
Pre
-cut w
ith
cross w
hich
re
du
ces tip
or
pro
be
be
com
ing
clo
gg
ed
Ga
s pe
rme
ab
le
pro
vidin
g
ba
rrier to
b
acte
ria &
o
the
r solid
co
nta
min
an
ts
Ga
s pe
rme
ab
le
tha
t allo
ws a
ir a
nd
CO
2
excha
ng
e, b
ut p
reven
ts m
oistu
re
evap
ora
tion
.
Air-O
-Se
al h
as a
mu
ch lo
we
r
mo
isture
tra
nsfe
r rate
th
an
ou
r Ga
s P
erm
ea
ble
A
dh
esive
Se
al
(4ti-0
515
) an
d
is suita
ble
for
ba
cteria
l or ce
ll cu
lture.
Hig
h so
lven
t re
sistan
ceM
ed
ium
stre
ng
th
tran
spa
ren
t se
al
Th
in g
as
pe
rme
ab
le, m
oistu
re
ba
rrier se
al.
Allo
ws a
ir an
d
CO
2
excha
ng
e
bu
t preve
nts
evap
ora
tion
.
2 se
alin
g
surfa
ces
Op
tical w
ind
ow
s
Min
Tem
p-2
0°C
-20
°C-8
0°C
-40
°C-8
0°C
-20
°C-2
0°C
-20
°C-2
0°C
-20
°C0
°C-2
0°C
Ma
x Tem
p110
°C110
°C110
°C12
0°C
110°C
80
°C8
0°C
30
°C8
0°C
80
°C4
0°C
110°C
Ste
rile•
•
Pie
rcab
le•
••
••
Pe
ela
ble
••
••
••
••
••
••
RN
ase
/ Dn
ase
free
••
••
••
••
••
••
Me
asu
rem
en
ts
(mm
)*
135
mm
x 80
mm
133
mm
x 76m
m14
0m
m x 7
7m
m13
0m
m x 8
0m
m13
5m
m x 7
7m
m13
5m
m x 8
0m
m13
7m
m x 7
7m
m13
5m
m x 8
0m
m12
2m
m x 8
0m
m13
0m
m x 8
0m
m13
5m
m x 8
3m
m14
8m
m x 9
8m
m
100
she
ets
4ti-0
50
04
ti-05
65
4ti-0
56
04
ti-05
50
4ti-0
56
64
ti-05
154
ti-05
164
ti-05
174
ti-05
124
ti-05
104
ti-05
18
4ti-0
519
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