Currently Seeking Employment

Opportunities in business development

and sales

Contact Rafael Casiano at 813-330-

7555rafaelcasiano2@

gmail.com

Top Commercial performer in 2016Built a very strong funnel and generated $8M

in contracts in Year 2018                        Increased the # of customers from 15 in 2015

to 40 in 2016Exceeded 2016 plan by $3MFinished 2016, 3x higher revenue than 2015 Increased the # of customers from 3 in 2014 to

15 in 2015Double sales in 2015 vs. 2014

Accelerate Discovery and Development from a Single Slide

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Challenges in Diagnostics Discovery and Development

Limited patient tissue

for comprehensiv

e analysis

Implications• Rapid sample depletion

• Insufficient data for stratification research

• Therapeutic options limited for patients

Protein Biomarkers• Protein expression• Phosphorylation • Ligands / Growth factors• Cell types

Genomic Biomarkers• DNA• RNA• miRNA• Sequencing

Complex Disease Biology• Interactions of multiple

pathways• Tumor heterogeneity• Adaptive resistance

MultiOmyx enables comprehensive profiling, improved diagnosis, and therapeutic options.

Immunoprofiling-Hot Application for MultiOmyx

Immune classification color blends 

CD4 CD8 CD68 PD-L1 pan-CK CD68|PD-L1

 

CD4 CD8 CD68 PD-L1 pan-CK

 

Immunofluorescence color blends 

MultiOmyx enables profiling the types of immune cells present in the tumor

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Serial Sections (5µm)

FFPE Block

(10-30 µm)

Single Ab staining

CD4CD8CD19CD45ROKi67

Different sections of a tissue being analyzed for different biomarkers may lead to different results!

Challenges with assessing immune markers co-expression in singleplexed assay

Co-expression Analysis

MO Multiplexed assay enables analysis of multiple immune markers co-expression from a single slide

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Hyperplexed: stain, image, erase and repeat

Dye Inactivation>60

Proteins

PD-L1 PanCK

PD-L1 PanCK

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CD8+ Ki67+

CD4+ CD45RO+ CD8+ CD45RO+

CD8+ Granzyme B+CD8+ PD1+

“Memory” T Helper “Memory” TC

“effector” TC“Proliferating” TC “Anergic” TC

Immune cell co-expressionCD4+FoxP3+  “Regulatory” T cell

Immune Cell Co-localization

PanCK CD3 CD4 CD8 FoxP3 PD-L1 CD68

Tumor/PanCK+

CD3+CD4+

T helper

CD3+CD4+FoxP3+

T regulatory

PD-L1+CD68+

CD3+CD8+

T cytotoxic

Tumor Margin

Innate (tumor cell intrinsic) resistance Adaptive resistance

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VisualizationBiomarker overlays

Biomarker co-expression

• Visualize co-localization of markers

• Generate .png images for publications and presentations

• Evaluate biomarker co-expression

Biomarker heat maps

• Visualize heat maps of multiple biomarkers for assessment of expression and co-localization

 

Biomarker clusters

• Visualize results of cluster analysis and explore the biosignatures

Molecular H&E Molecular DAB

• Conversion of fluorescent image to a H&E appearance. Allows interpretation in context of tumor

• Conversion of fluorescent image to DAB appearance. Familiar format for pathologist

  Cluster ID

Low Immune

Biomarker

Cell Count

s

Density in

Tumor (#/mm

2)

Density in

Stroma (#/mm

2)Tumor Cells 1386 NA NA

CD4+ 64 23.3 177.4CD8+ 47 11.7 134.6

CD20+ 13 7.8 33.6FOXP3+ 53 7.8 156.0PDL1+ 0 0 0

Biomarker

High Immune

Cell Count

s

Density in

Tumor (#/mm2

)

Density in

Stroma (#/mm2)

Tumor Cells 1692 NA NACD4+ 231 238.1 675.4CD8+ 299 492.3 547.0

CD20+ 14 2.7 61.8FOXP3+ 177 160.5 556.5PDL1+ 234 246.1 675.4

Hig

h Im

mun

eLo

w Im

mun

e

Multiplexed IF Analytics Output

CK CD4 CD8 CD20 FOXP3 PD-L1

CK CD4 CD8 CD20 FOXP3 PD-L1

CK CD4 CD8 CD20 FOXP3 PD-L1

CK CD4 CD8 CD20 FOXP3 PD-L1

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Immune Infiltration

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MultiOmyx Tumor Infiltrating Lymphocyte Panel

O Provides quantitative analytical results and spatial information of 12 cancer immune markers from every cell on a single FFPE

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Dyes are cycled by a proprietary chemical inactivation methodGerdes et al (2013) PNAS

Multiplexed ProteinsProtein + DNA FISH

CD4 CD8 CD68 PD-L1 pan-CK 

DNA Sequencing (ROI)Immune Phenotyping

- Up to 60 protein biomarkers can be fluorescently imaged in a single FFPE tissue section

- Additional “omics” such as RNAScope, DNA FISH, and NGS can be performed from the same IF processed slide and analyzed with protein data

- Advanced visualization of immune biomarker co-expression and co-localization

- Sophisticated algorithms identify individual cells and measure quantitative per-cell biomarker and morphology profiles

MultiOmyx Technology Summary…

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