Sequence Automation

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In This Section, We Will Discuss:

How to set up a sequence.

How to perform automated recalibrations.

How to do bracketing.

How data files are named.

How to use Sequence Summary.

How to run the sequence.

The sequence container (folder).

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Building a Sequence for Automated Analysis

1. Go to the Method and Run Control view.

2. Go to View > Preferences and set up sequence folder naming convention.

3. From the Sequence menu, select New Sequence.

3. Edit the Sequence Parameters.

4. Edit the Sequence Table.

5. Select a Sequence Output.

6. Save the Sequence

7. Run the Sequence form the RunControl menu or the Sequence Table.

Note: Hypersequence is a feature for the Agilent 1100/1200Series Well plate Handler only.

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Preferences

•Specifies naming convention for sequence data containers.

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Sequence Parameters

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Explicit Calibration

CalibStandards

CalibStandards

CalibStandards

UpdateCalibration

UpdateCalibration

UpdateCalibration

Samples Samples

CalculateSample

AmountsAfter EachInjection

CalculateSample

AmountsAfter EachInjection

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Sequence Table Explicit Recalibration

Additional Columns inSequence Tables:

•Sample Amount•ISTD Amount•Multiplier•Dilution •Data file•Inj Volume•Lims ID•AutoBalance

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Customize Sequence Table

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Explicit Plus Cyclic Recalibration

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Bracketing Calibration

CalibStandards

CalibStandards

CalibStandardsSamples Samples

UpdateCalibration

CalculateSample

AmountsFor EachInjection

Injection

UpdateCalibration

CalculateSample

AmountsFor EachInjection

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Bracketed Sequence Operation

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Partial Sequence Preview of Bracketing

Will show you the order of injections.

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Fill in Sequence Table Using Sequence Table Fill Down Wizard

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Sequence Output

A Sequence Summary Reportallows you to select from asimple one page Sample Summary to a comprehensivereport documenting all aspectsof the analysis including cross-sample statistics.

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Edit Sequence Summary

Statistics calculated inStandard or Extendedformat.

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Extended Statistics

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Save the Sequence File

Save Current Sequence

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Sequence Sampling Diagram

Shows sequence tray set-up and sequence processing status. Click to set-up sequence. Select Start tobegin sequence.

Select from the View menu.

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Start a Sequence

•RunControl menu•Start button•F6•Sequence Table•Partial Sequence Table

•When you start a sequence, a sequence folder is created in the DATA directory.

•The sequence folder is named according to the subdirectory specified in Sequence Parameters, and the naming convention specified in Preferences.

•A copy of the master methods (Chem32\1\Methods\names.M) and sequence template file (Chem32\1\Sequences\name.S) are copied to the folder. The sequence acquisition is run from these copies.

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Sequence Folder Data Structure

Sequence Container (Folder)Subdirectory sequencetemplatename year-month-day hour-minute-second

C:\Chem32\1\DATA\03-15-06\CLASSSEQUENCE 2006-03-15 10-46-09

Copy of Sequence Template

Copy of Master Method

Acquired Data

Batch file for Batch Review

Sequence Logbook

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Sequence Data Files

Format for Automatic Injection: 003-0101003 Vial Number01 Sequence Line01 Replicate

PrefixPrefix000001Prefix000002, etc. for a total of 15 characters

Format for a Cyclic Calibration Standard: C1-02001C Indicates a cyclic calibration standard was used1 Calib Line number02 Sequence Line001 Replicate

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Recalibration Settings

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Calibration Report Options