Softacid Aqua

BY-PRODUCTS preservation by acid´s applications

ZOOTEXNIA S.A. Address: 10 Nikomidias str.  Peristeri, Athens P.O.: 12134  Tel.: +30 210 5766048 Fax: +30 210 5766261 Email: zootex@otenet.gr | www.zootexnia.com

PRODUCTION PROCESS

By Product(70-80 % Moisture)

COOKERCOOKER(Protein coagulation)

Cooked cake

(2-3% Moisture)

PRESSPRESS(Liquid and fat

release)

FILTERFILTER

Pressed cake

Meat meal Meat meal (sale)

Fat with suspended

liquidsWater

Sludge from tricanter

Water residue

(Treatment and disposal)

TRICANTERTRICANTER

(centrifugation)

Transformation in category II

Oil Oil (Sale)

Meat by-product

BACTERIA FLORA

Time

Particle size

Temperature

Water activity

Free fatty acids

HIGH ENZYMATIC

ACTIVITY(decarboxylase)

Biogenic Amines

Ammonia

Analytical values

High Oleic acidity

High Histamine

level

High TVN Level

EVOLUTION OF MEAT MEAL STABILITY

Bacteria load

DIFFERENT CATEGORIES OF BIOGENIC AMINES

Histamine Tryptamine

TryptophaneHistidine

Hetero Cyclic

Tyramine phenylethylamine

PhenylalanineTyrosine

Cyclic

Putrescine Cadaverine

LysineOrnithine Arginine

Diamine

Spermine

SpermidinePolyamine

MEAT by-product

Time

Particle size

Temperature

Bacteria load

Free fatty acids

Biogenic Amines

Ammonia

BACTERIA FLORA

LOWENZYMATIC

ACTIVITY

Organic acid environment

Reduction of bacterial flora development

Reduction of enzymatic activity

Analytical values

Low Oleic acidity level

Low Histamine

level

Low TVN Level

EVOLUTION OF MEAT MEAL STABILITY

Water activity

Softacid Aqua

Slaughterhouse´s byproducts have a perfect nutritional and microbiological quality for the manufacture of meat meal.

Until they reach the factory of meat meal they are degrading. Factors: temperature, humidity, etc.

Slow down this process: COLD: PRESENT.

Theorically in the slaughterhouse

ACID: FUTURE. Perfect preservations from 6 months – 1 year.

Usual storage conditions before truck loading

Usual storage conditions before truck loading

SOLUTION IN NORWAY SILAGE (MILLING* of by-products

with acid) from slaughterhouse by-products of salmon and trout. The product will be preserved during more than one month.

(*) Silage needs an important investment

SOLUTION IN CHILE

SILAGE (MILLING* of by-products with acid) from dead fishes.

The product can be preserved during more than six months.

(*) Silage needs an important investment

WE OFFER A NEW SOLUTION

Development since 2011 with many trials in different factories

Preservation of solid by-products from fish, meat and food industriesWithout coldWithout milling

TRIAL IN SPAIN / MARCH-JULY 2011

SLAUGHTERHOUSE Nº 1(Data obtained from dry matter) WITH Softacid Aqua WITHOUT ACID

pH 3.49 5.74

TVN (mg N/100 g échantillon) 0.02 0.19

OLEIC ACIDITY(%) 9.4 53.6

PUTRESCINE (ppm) 43 849

CADAVERINE (ppm) 16 1.062

HISTAMINE (ppm) 18 683

ENTEROBACTERS (ufc/g) < 103 2.1 x 108

Applying acids for the preservation of byproducts. We poured an acid almost at the same time that

the production of byproducts in the silo.

Comparative between two samples of 300 g of poultry intestines taken from the silo and analysed 20 hours later in the laboratory

Sampling Days 14-april 05-may 12-may 19-may 26-may 02-june

Acid dose (Kg/t) 10 10 10 5 5 10

pH at the reception in factory (Higienizo result.) (*1)

  5,19 5,37 5,27 5,6 4,3 (*4)

pH at the reception in factory (customer result) (*1)

  5,13 5,5 5,3 6,1 4,8

pH in laboratory (*1) 6,1 5,68 5,94 5,7 6,16 4,1

Oleic Acidity (%) (* 1) 30,3 12,7 69 74,3 86,5 12,4

Oleic Acidity (%) (* 2)   4 4 4,45 3,7 7 (*3)

TVN (g N2/100 g) 0.290 0,16 0,22 0,25 0,43 0,059

Peroxids (meq/Kg) 0,4 49 0,1 2 6,4 20

Formic Acid(%) 0,24 0,15 0,34 0,25 - 0,89

Putrescine (ppm) 1167 1167 1360 5610 4691 142

Cadaverine (ppm) 1333 1330 1506 6319 4775 258

Histamine (ppm) 145 149 236 290 300 73

Enterobacters (ufc/g) 3,5 x 107 3,5 x 107 3,5 x 106 1,65 x 108 3,5 x 107 9,5 x 105

(* 1) – fresh matter in truck.(* 2) – Fat obtained after the manufacturing process of meat meal. (* 3) – Power stop during the manufacturing process of meat meal.(*4) – Lower pH due to retention of exudate

TRIAL IN PORTUGAL (February 2013)

2 batches of by-products from poultry slaughterhouses.

Temperature 10-15 ºC:

Intervalle collect/ Processing

(hours)Oleic acidy

Control 5 3.55

10Kg/T Softacid Aqua 24 2.9

TRIAL IN PORTUGAL (May-June 2013)

SLAUGHTERHOUSE C

SLAUGHTERHOUSE A1

SLAUGHTERHOUSE A2

SLAUGHTERHOUSE A3

SLAUGHTERHOUSE B1

SLAUGHTERHOUSE B2

SLAUGHTERHOUSE B3

TRUCK A Acidification

25 T/day 20 T/day 25 T/day

TRUCK B No acidification

20 TAcidified

25 T Non Acidified

8.33 T/day

8.33 T/day

65% acidified

8.33 T/day

35% non acidified

Acidified by layer in the truck Acidified in the mass

Remark: Acid application problem in Slaughterhouse C begin end of Friday 5 and during Monday 8 and Tuesday 9 (valve was broken down)Good control with 65% of treated by-product but not sufficient with only 35%After the trial, Truck B also equiped to tread the by products of Slaugher houses B1 B2 and B3

(30-40 ºC at noon)

TRIAL IN PORTUGAL (May-June 2013)

Acidification Day Daily Average

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Monday

1 6,4

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Tuesday

2 4,4

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Wednesda

y 3 4,9

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Thursday

4 4,6

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Friday

5 6,8

Acidification Day Daily Average

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Monday

8 11,1

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Tuesday

9 9,3

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Wednesday

10 5,2

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Thursday

11 5,1

Slaughter Houses B1

B2 B3

Slaughter house A1 A2

A3 Truck A

Slaughter House C

Friday

12 5,7

Resullts on Oleic acidity during the 4 summer months

June July August September

2012 2013 2012 2013 2012 2013 2012 2013

Monthly average 9,9 5,8 9,7 6,1 9,5 7,3 8,1 6,4

First week 9,2 6,8 13,7 5,1 10,6 6,5 9,5 6,2

Second Week 10,7 4,6 7,6 7,4 * 12,9 8,0 8 7,1

Third Week 8,8 5,9 9,8 5,3 8,6 ** - 8,4 5,9

Fourth Week 11,9 5,7 7,6 5,3 7,2 ** 7,2 - -

* This value is the average value of the offal Monday-Tuesday** The offals of this week was rejected as unacceptable

TRIALS IN MOROCCO (A - January 2013)

120 T of fish by products on thursday Processing on monday morning (84-107h) Time elapsed untill the fish was processed was

84-107 hours.

Histamine ppm

Control 1700

Softacid Aqua 3.5 Kg 920

TRIALS IN MOROCCO (A – February 2013) 163 tons of fish and around 12 tons of byproducts.

Acid application on Sunday and the by products were delivered to the meal factory during an average tranportation time (10-11 hours).

Fishes and fish byproducts were processed the following day.

Histamine ppm Increase vs Frozen

No acidification 1000 X 10.5

Softacid Aqua10 Kg/T 295 X 3.1

Frozen fish (Control) 95 0

TRIALS IN CHILE A. November 2012

A test took place applying acid over trout fishery sludge in order TO ELIMINATE THE BAD ODOURS. The composition of the sludge was about 50% faeces, 25% water, 15% undigested feed and 10% fat.

Average temperature of the test was 20-25 ºC.

Samples of 100 Kg 0 days

60 days

90 days

NON acidified samples 5,4 6.0 6.0

Acidified samples (35 l/ton)

3,33.3

3.4

Samples of 1000 Kg 0 days

60 days

90 days

NON acidified samples

5,4 6,5 6,5

Acidified samples(35 l/ton)

3,3 3,3 3,5

In both acidified samples the smell dissapeared in less than 60 seconds.

(As close as 30 cm from the sample, no odour is detected)

TRIALS IN CHILE A. November 2012

B. TEST IN SALMON SLAUGHTERHOUSE, OVER SALMON INTESTINES (50 Kg cu)

Batch 1: 50 Kg of salmon guts WITH NO ACIDS Batch 2: 50 Kg of salmon guts ACIDIFIED with 10 Kg/Tm of Softacid

Aqua.

Results

Sample identification Histamine Cadaverine Putrescine Oleic acidity

%

TVN mg N/100g

pH % T0 %

With treatment Time 0Without treatment Time 0

6824

71335

7234

5,702,89

14,6813,30

6,493,26

15,216,5

With treatment Time 6Without treatment Time 6

10119

73723

12327

7,713,17

31,2311,53

6,253,46

17,616,5

With treatment Time 12Without treatment Time 12

11117

85825

30326

8,492,99

44,0613,53

6,293,63

19,918,4

With treatment Time 18Without treatment Time 18

14618

94233

42326

9,783,14

56,9813,36

6,263,78

19,918,5

SOFTACID AQUA APPLICATIONIN THE SLAUGHTERING HOUSE

Spraying system in factory

SOFTACID AQUA APPLICATIONIN THE TRUCK