STAININGSTAINING

R.SEENIAMMALR.SEENIAMMALAEH, THENIAEH, THENI

PURPOSEPURPOSE

TO STAIN THE EPITHELIUM OF THE TO STAIN THE EPITHELIUM OF THE CORNEA TO SEE CORNEA TO SEE

FOR THE VIABILITY OF THE FOR THE VIABILITY OF THE EPITHELIUM OR CONTINUITY OF EPITHELIUM OR CONTINUITY OF EPITHELIUM EPITHELIUM

TYPES OF STAINTYPES OF STAINFLUORESCEINFLUORESCEIN

1MG FLUORESCEIN STRIPS 1MG FLUORESCEIN STRIPS AVAILABLEAVAILABLE

DETECTS DISCONTINUITY DETECTS DISCONTINUITY IN EPITHELIUMIN EPITHELIUM

ULCER, ABRASION,SPKS+ ULCER, ABRASION,SPKS+ STAINS FLUORESCEIN STAINS FLUORESCEIN GREEN COLOUR GREEN COLOUR

ROSE BENGALROSE BENGAL DETECTS NON- VIABLE OR DETECTS NON- VIABLE OR

DEAD EPITHELIAL CELLS.DEAD EPITHELIAL CELLS. EG. DRY EYE, NECROSIS.EG. DRY EYE, NECROSIS. STAINING REDSTAINING RED SIDE EFFECT SEVERE SIDE EFFECT SEVERE

IRRITATIONIRRITATION SO ROSE BENGAL NOT SO ROSE BENGAL NOT

USED USED

PROCEDUREPROCEDURE TEAR AND OPEN THE STAIN STRIP.TEAR AND OPEN THE STAIN STRIP. DO NOT TOUCH THE STAINED PART DO NOT TOUCH THE STAINED PART

OF STRIP.OF STRIP. IF TOUCHED DISPOSE (1MG OF IF TOUCHED DISPOSE (1MG OF

FLUORESCEIN)FLUORESCEIN) ADD 1DROP OF ARTIFICIAL TEARS ADD 1DROP OF ARTIFICIAL TEARS

TO THE STAINED STRIP & WAIT FOR TO THE STAINED STRIP & WAIT FOR FEW SECONDS.FEW SECONDS.

EXPLAIN THE PROCEDURE TO THE EXPLAIN THE PROCEDURE TO THE PATIENT THAT YOU ARE TESTING PATIENT THAT YOU ARE TESTING AND NOT TREATING.AND NOT TREATING.

TELL THE PATIENT TO LOOK UP AND TELL THE PATIENT TO LOOK UP AND APPLY THE STAIN STRIP IN THE APPLY THE STAIN STRIP IN THE LOWER PALPEBIAL CONJUNCTIVA.LOWER PALPEBIAL CONJUNCTIVA.

EXAMINE THE CORNEA UNDER A EXAMINE THE CORNEA UNDER A COBALT BLUE FILTER IN SLIT LAMP .COBALT BLUE FILTER IN SLIT LAMP .

AREAS WHERE THE IS AREAS WHERE THE IS DISCONTINUTY OF EPITHELIUM IS DISCONTINUTY OF EPITHELIUM IS STAINED FLUORESCEIN GREEN. STAINED FLUORESCEIN GREEN.

PRECAUTIONSPRECAUTIONS SEE THE CORNEA BEFORE STAINING SEE THE CORNEA BEFORE STAINING

IN THE SLIT LAMP BIOMICROSCOPY IN THE SLIT LAMP BIOMICROSCOPY TO GET AN IDEA WHERE TO LOOK TO GET AN IDEA WHERE TO LOOK AFTER STAINING .AFTER STAINING .

EXAMINE AC FOR CELLS/ FLARE EXAMINE AC FOR CELLS/ FLARE BEFORE STAINING SINCE IT IS BEFORE STAINING SINCE IT IS DIFFICULT TO SEE AFTER STAINING.DIFFICULT TO SEE AFTER STAINING.

INDICATIONSINDICATIONS

! 1. INJURY CASES – TO MAKE OUT EPITHELIAL ! 1. INJURY CASES – TO MAKE OUT EPITHELIAL DEFECT.DEFECT.

2. IN PENETRATING INJURY AFTER STAINING SEE 2. IN PENETRATING INJURY AFTER STAINING SEE WHETHER STAIN IS THERE OR WASHED AWAY WHETHER STAIN IS THERE OR WASHED AWAY TO SEE WHETHER IT IS FULL THICKNESS TEAR. TO SEE WHETHER IT IS FULL THICKNESS TEAR.

SIEDELS TEST SIEDELS TEST !!. ULCER CASES – NEW- REVIEW (TO SEE !!. ULCER CASES – NEW- REVIEW (TO SEE

WHETHER HEALING.WHETHER HEALING. !!!. DRY EYE – FOR SPKS!!!. DRY EYE – FOR SPKS !V. FOR APPLANATION – PUT A DROP OF TOPICAL !V. FOR APPLANATION – PUT A DROP OF TOPICAL

ANAESTHETIC 4% XYLOCAINE AND APPLY STAINANAESTHETIC 4% XYLOCAINE AND APPLY STAIN

JONES TEST JONES TEST TO TEST PATENCY OF LACRIMAL TO TEST PATENCY OF LACRIMAL

PASSAGE.PASSAGE. APPLY STAIN IN BOTH EYES AND TEST APPLY STAIN IN BOTH EYES AND TEST

THE NASAL CAVITY WITH COTTON THE NASAL CAVITY WITH COTTON WIPER INTRODUCED INTO IT .WIPER INTRODUCED INTO IT .

IF FLUORESCEIN IS PRESENT IN COTTON IF FLUORESCEIN IS PRESENT IN COTTON WIPER WHILE REMOVING THE WIPER WIPER WHILE REMOVING THE WIPER INDICATES LACRIMAL PASSAGE IS FREE. INDICATES LACRIMAL PASSAGE IS FREE.