start your microrna discovery from a single...
TRANSCRIPT
Contact our research specialists to learn more North America: +1 781 376 4150 • Rest of world: +45 45 650 929 [email protected] • www.exiqon.com
Amplifi cation of a synthetic microRNA was performed using the miRCURY™
LNA microRNA PCR system. Hsa-miR-100 (2 × 108 copies) input was used
to generate cDNA. A ten-fold serial dilution ranging from 2 × 109 copies to
2 copies cDNA was amplifi ed by real-time PCR. A. The amplifi cation curves
and B. the standard curve show excellent linear correlation between the
decreasing cycle numbers and the logarithm of the microRNA copy number,
proving the assay enables sensitive microRNA detection down to less than
10 copies with a wide dynamic range of at least 8 logs.
Accurate quantifi cation over a wide dynamic range of microRNA copy numbers
Marie-Louise Lunn, Ph.D.
0.1
1
10
100
0 10 20 30 40 50
Fluo
resc
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Cycle number
Cycl
e nu
mbe
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0
10
5
15
20
25
30
35
40
10 102 103 104 105 106 107 108 109 1010
microRNA copies
R2 = 0.994Amplification efficiency 100%
2 x 109 copies
2 copies
A
B
Start your microRNA discovery from a single cell MicroRNAs are small wonders. To study them, life science
researchers need extremely accurate and sensitive tools
for detection and quantitation.
With the new miRCURY™ LNA microRNA PCR system,
your discovery starts from 10 pg total RNA – you only need
a single cell. You can even detect high and low expressed
microRNAs from the same sample in your experiment, as
the dynamic range is 8 logs wide.
Our PCR system combines microRNA-specifi c primers
and SYBR® Green detection and is part of Exiqon’s popular
miRCURY™ LNA product line, the most complete range of
tools for microRNA analysis available today.
The miRCURY™ LNA tools enable you to seek, fi nd and
verify microRNAs. Accelerate your discoveries.
Marie-Louise Lunn, Ph.D., Product Manager
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To order or request additional information, please visit our Web site or:Call: 1-800-843-4388 (Continental US and Canada) 516-422-4100 (All other locations)FAX: 516-422-4097 E-mail: [email protected]: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924
CONTENTS
PART 1: CHEMISTRY ANDGENETICS
1. The Mendelian View of the World2. Nucleic Acids Convey Genetic
Information3. The Importance of Weak Chemical
Interactions4. The Importance of High-Energy
Bonds5. Weak and Strong Bonds
Determine MacromolecularStructure
PART 2: MAINTENANCE OFTHE GENOME
6. The Structures of DNA and RNA
7. Genome Structure, Chromatin,and the Nucleosome
8. The Replication of DNA9. The Mutability and Repair of
DNA10. Homologous Recombination at the
Molecular Level11. Site-Specific Recombination and
Transposition of DNA
PART 3: EXPRESSION OF THEGENOME
12. Mechanisms of Transcription13. RNA Splicing14. Translation15. The Genetic Code
PART 4: REGULATION
16. Transcriptional Regulation inProkaryotes
17. Transcriptional Regulation inEukaryotes
18. Regulatory RNAs19. Gene Regulation in Development
and Evolution20. Genome Analysis and Systems
Biology
PART 5: METHODS
21. Techniques of Molecular Biology22. Model Organisms
INDEX
By James D. Watson, Cold Spring Harbor Laboratory, Tania A. Baker,Massachusetts Institute of Technology, Stephen P. Bell, Massachusetts Institute of
Technology, Alexander Gann, Cold Spring Harbor Laboratory, Michael Levine, University ofCalifornia, Berkeley, and Richard Losick, Harvard University
This sixth edition of James D. Watson’s classic textbook Molecular Biology of the Gene has beenthoroughly revised and updated. Accessible to anyone interested in molecular biology and genet-
ics, the book provides a historical basis for the field, concise descriptions of fundamental chemicalconcepts, a comprehensive survey of genome maintenance and expression, and a discussion of stan-dard techniques and model organisms commonly used in molecular biology studies. It includes allnew chapters on the regulatory RNAs and genomics and systems biology. The book has an accompa-nying Web site www.aw-bc.com, which contains interactive tutorials, animations, and critical-think-ing exercises designed to help students explore and visualize complex concepts.
2008, 841 pp., illus., indexHardcover $151 ISBN 978-080539592-1
This book is co-published with Benjamin Cummings. Requests for textbook examination copies, orders for course adoptions, and ALL individual orders outside of the U.S. should be sent to Benjamin Cummings at www.aw-bc.com.
The Power to Question
© 2006-2007 Santa Cruz Biotechnology, Inc., all rights reserved. "Santa Cruz Biotechnology" and the Santa Cruz Biotechnology, Inc. logo are registered trademarks of Santa Cruz Biotechnology, Inc.
santa cruz biotechnology, inc.
cell signaling research antibodies and siRNA gene silencers
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Zoom in on new targetsin cancer research
LightCycler® 480 Real-Time PCR System
Today’s cancer research requires increasingly accurate and versatile tools to
shed light on genetic and epigenetic variation patterns. Choose the
LightCycler® 480 System’s powerful combination of innovative hardware,
high-performance reagents, and cutting-edge analysis software to drive
discovery using applications such as:
■ High-Resolution Mutation Screening
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■ Quantitative ChIP Analysis
■ miRNA Analysis in B-Cell Lymphoma
■ Analysis of Transcriptional Repression
View recently published studies using the LightCycler® 480 System for these
and other applications at www.lightcycler480.com
For general laboratory use. Not for use in diagnostic procedures.
This LightCycler® 480 Real-Time PCR System is licensed under U.S. Patent 6,814,934and corresponding claims in its non-U.S. counterparts and under one or more of U.S.Patents Nos. 5,038,852, 5,656,493, 5,333,675, or corresponding claims in their non-U.S.counterparts, for use in life science, by implication or by estoppel under any patentclaims or for any other implication. The product is covered in-part by US 5,871,908, co-exclusively licensed from Evotec OAI AG. Parts of the Software used for theLightCycler® 480 System are licensed from Idaho Technology Inc., Salt Lake City, UT,USA. LIGHTCYCLER and HRM are trademarks of Roche. Other brands or productnames are trademarks of their respective holders.
© 2008 Roche Diagnostics GmbH. All rights reserved.
Analysis of tumor suppressor gene methylationwith the LightCycler® 480 System. Mixtures ofunmethylated and fully methylated genomic DNAwere treated with bisulfite. A fragment of thePPP3CC tumor suppressor gene was then amplifiedand analyzed by high-resolution melting. Thisallowed semi-quantitative determination of theportion of methylated DNA contained in each case.
Roche Diagnostics GmbHRoche Applied Science68298 Mannheim, Germany