Scholars -1 Subject- Biotechnology Session 2019-20 1 S

Subodh Public SchoolRambagh

Session:-2019-2020 Subject-Biotechnology

Subject Code –045

Scholars-1

An unprecedented growth of human knowledge in the field of Biological Sciences coupled with

equally significant developments in the field of technology have brought significant changes into

existing social and economic systems. The emerging field of Biotechnology is likely to further

enhance the applications of Science and Technology in the service of human welfare. Modern

Biotechnology processes encompass a wide range of new products such as antibiotics, vaccines,

monoclonal antibodies and many more. Furthermore, developments in recombinant DNA

technology have yielded numerous new useful products in the fields of healthcare and agriculture.

The present syllabus takes care of all these aspects. Due emphasis has been laid on familiarizing

the learners with the fundamental concepts, basic techniques and their applications. It is expected

that the knowledge gained through the study of different topics and the skills acquired through the

prescribed practical work will make the learners competent to meet the challenges of academic as

well as professional courses after studying the subject at senior secondarystage.

Objectives

The broad objectives of teaching Biotechnology at senior secondary level are to:

help the learners know and understand basic facts and concepts of the subject at

elementarystage.

expose the students to different basic processes and basic techniques used in

Biotechnology.

familiarize the learners to understand the relationship of the subject to health, nutrition,

environment, agriculture and industry,etc.

develop conceptual competence in the learners so as to cope up with professional courses

in futurecareer.

acquaint students with different applications of Biotechnology in everydaylife.

develop an interest in students to study biotechnology as adiscipline.

School Exam Syllabus

Name of Book :A Text Book of Biotechnology for class XI Author/Publisher : C.B.S.E. New Delhi Ref. Book (ANY ONE) :Biotechnology for class XI by S. ChandPublications Biotechnology for class XI by Kalyani Publication. Practical Book : A practical approach to Biotechnology for class XI by C.B.S.E. Name of Facilitator : Dr. Manish Jain- (HOD) 94133-44952

Scholars -1 Subject- Biotechnology Session 2019-20 2 S

Unit Test 1 [25 Marks] S.

No. Name of Topic Activity No. of Periods Weightage

1 Unit-I Introduction to Biotechnology

A. Sterilization techniques (Wet And Dry Sterililzation, Chemical Sterilization and ultra filtration)

B. Fermentation – Activity by Yeast.

10

07

2 Unit-III Cell &Devlopment.

a. The basic unit of life

a) Preparation of temporary slide of Onion peels to observe plant cell and its component.

20

18

Half Yearly Exam - Theory [70 Marks]

S. No. Name of Topic Activity No. of Periods Weightage 1 Unit-I Introduction to

Biotechnology A. Sterilization techniques (Wet And Dry Sterililzation, Chemical Sterilization and ultra filtration)

B. Fermentation – Activity by Yeast.

10

10

2 Unit-III Cell &Devlopment.

b. The basic unit of life

a) Preparation of temporary slide of Onion peels to observe plant cell and its component.

20

30

3 Unit-III

Cell &Devlopment.

b. Cell and Development.

a )Determination of bacterial growth curve by the help of spectrophotometer a )Cell Counting with the help of haemocytometor. b) Microscopy and its components c ) Cell fractionation

d ) Cell growth measurement by turbidity, absorbance, dry weight determination.

30

30

Half Yearly Exam - Practical [30 Marks] S. No. Name of Topic Weightage

1 2 Experiments 10x2=20

2 Record of Experiments and Viva 05

3 Investigatory Project and Viva 05

Unit Test 2 [25 Marks]

S. No.

Name of Topic Activity No. of Periods Weightage

1 Unit IV Genetics and Molecular Biology

a) Study Mendelian inheritance using seeds of different colour/seed of any plant. b) Exercise on controlled pollination Emasculation, tagging and bagging. c )Study of self pollination and Cross pollination. d )Study prepared pedigree charts of genetic traits such as rolling of tongue, blood groups, widow's peak, and colour blindness.

20

25

2 Genome Function

Prepare a power point presentation on genome function a.Isolation of genomic DNA. b Staining of nucleic acids. c Karyotyping – Preparation of Karyotype. d Bacterial recombination – Conjugation..

22

25

Scholars -1 Subject- Biotechnology Session 2019-20 3 S

Final Exam Theory [70 Marks]

S. No.

Name of Topic Activity No. of Periods

Weightage

1 Unit-I Introduction to Biotechnology

A. Sterilization techniques (Wet And Dry Sterililzation, Chemical Sterilization and ultra filtration) B. Fermentation – Activity by Yeast.

10

05

2 Unit-III Cell &Devlopment.

c. The basic unit of life

a) Preparation of temporary slide of Onion peels to observe plant cell and its component.

20

10

3 Unit-III

Cell &Devlopment.

b. Cell and Development.

a )Determination of bacterial growth curve by the help of spectrophotometer a )Cell Counting with the help of haemocytometor. b) Microscopy and its components c ) Cell fractionation d ) Cell growth measurement by turbidity, adsorbance, dry weight determination.

30

10

4 Unit IV Genetics and Molecular Biology

a) Study Mendelian inheritance using seeds of different colour/seed of any plant. b) Exercise on controlled pollination Emasculation, tagging and bagging. c )Study of self pollination and Cross pollination. d )Study prepared pedigree charts of genetic traits such as rolling of tongue, blood groups, widow's peak, and colour blindness.

20

10

5 Genome Function

Prepare a power point presentation on genome function a.Isolation of genomic DNA. b Staining of nucleic acids. c Karyotyping – Preparation of Karyotype. d Bacterial recombination –

Conjugation..

22

15

6 Unit I – Bio molecules

Chapter – Building Blocks of Biomolecules Structure& Dynamics

A Searching of Biomolecular data base through internet web sites :- Nucleic Acids Proteins

14

10

7 Structure and function of Macromolecules

a. To study effects of Salivary amylase on starch. b. To study effect of temperature on salivary amylase. c Isolation of milk protein (Casein) d Estimation of protein.

14

10

Final Exam - Practical [30 Marks]

S. No. Name of Topic Weightage

1 2 Experiments 10x2=20

2 Record of Experiments and Viva 05

3 Investigatory Project and Viva 05

NOTE1: 1. Syllabus is subject to change as per new directives of CBSE and will be intimated well in time. 2. All suggested activities will be taken as per availability and feasibility conditions. At least 2 activities before Half Yearly and 2-3 activities before Final/Pre-board examination will be taken and in all 5 activities.

Scholars -1 Subject- Biotechnology Session 2019-20 4 S

CBSE Curriculum 2019-20

CLASS- XI (2019-20)

COURSE STRUCTURE

OnePaper Time: 3 hrs.

Max. Marks 70+30

Units Marks No. of Periods

Unit- I Biotechnology: An overview 5 20

Unit-II Molecules of Life 20 50

Unit-III Genetics and Molecular Biology 20 50

Unit-IV Cells and Organisms 25 60

Practical 30 60

Total 100 240

CLASS XI

(Theory)

OnePaper Time: 3hrs.

Total Marks: 70

Unit-IBiotechnology:Anoverview 5Marks

Chapter 1: Biotechnology: An Overview

Historical Perspectives, Technology and Applications of Biotechnology, Global market and Biotech

Products, Public Perception of Biotechnology, Biotechnology in India and Global Trends

Unit-II Moleculesof Life 20Marks

Chapter 1: Biomolecules: Building Blocks

Building Blocks of Carbohydrates - Sugars and their Derivatives, Building Blocks of Proteins - Amino

Acids, Building Blocks of Lipids - Simple Fatty Acids, Sphingosine,

Glycerol and Cholesterol, Building Blocks of Nucleic Acids - Nucleotides, Biochemical Transformations

Chapter 2: Macromolecules: Structure & Function

Carbohydrates - The Energy Givers, Proteins - The Performers, Enzymes - The Catalysts, Lipids and

Biomembranes - The Barriers, Nucleic Acids - The Managers

Unit-III Genetics andMolecularBiology 20 Marks

Chapter 1: Concepts ofGenetics

Historical Perspective, Multiple Alleles, Linkage and Crossing Over, Genetic Mapping, Gene Interaction, Sex-

Linked Inheritance, Extra nuclear Inheritance, Quantitative Inheritance, Genes at the Population Level

Chapter 2: Genes and Genomes: Structure and Function

Scholars -1 Subject- Biotechnology Session 2019-20 5 S

Discovery of DNA as Genetic Material, DNA Replication, Fine Structure of the Genes, From Gene to

Protein, Transcription – The Basic Process, Genetic Code, Translation, Regulation of Gene

Expression, Mutations, DNA Repair, Human Genetic Disorders, GenomeOrganization

Unit IV: CellsandOrganisms 25Marks

Chapter 1 The Basic Unit of Life

Cell Structure and Components, Tissues and Organs, Stem Cells, Biodiversity, Organization of Life

Chapter 2: Cell Growth and Development

CellDivision,CellCycle,CellCommunication,Nutrition,GaseousExchange,InternalTransport,MaintainingtheInternal

Environment, Reproduction, In Vitro Fertilization, Animal and Plant Development, Immune Response inAnimals,

ProgrammedCellDeath,DefenseMechanismsinPlants

PRACTICALS

Note: Every student is required to do the following experiments during the academic session.

1. Recording practical results and safety rules in thelaboratory

2. Preparation of buffers and pH determination

3. Sterilization techniques

4. Preparation of bacterial growthmedium

5. Determination of bacterial growthcurve

6. Cellcounting

7. Isolation of milk protein(Casein)

8. Estimation of whey protein by biuretmethod

9. Assaying the enzyme acidphosphate

10. Estimation of blood glucose by enzymatic and glucometermethod(GOD/POD)

11. Study of various stages of mitosis and calculation of mitoticindex

12. Preparation ofkaryotype

Scheme of Evaluation

Time:3Hours Max. Marks 30

The scheme of evaluation at the end of session will be as under:

Twoexperiments : 20 Marks

Vivaonexperiments : 5 Marks

Practical record : 5 Marks

BIOTECHNOLOGY (Code No. 045)

6

QUESTION PAPER DESIGN

Class – XI/XII (2019-20)

1) Board Examination – Theory

Time:3Hours Max. Marks: 70

S.

No.

Typology of Questions VSA

(1 Marks)

SA-I

(2 Marks)

SA-II

(3 marks)

LA

(5 marks)

Total

Marks

Percent

-age

1. Remembering- Exhibit memory of

previously learned material by

recalling facts, terms, basic concepts, and answers.

5 1 2 1 9 (18)

25.7%

2. Understanding- Demonstrate

understanding of facts and ideas by

organizing, comparing,translating,

interpreting, giving descriptions and

stating main ideas

4

1

2

1

8 (17)

24.3%

3. Applying: Solve problems to new

situations by applying acquired

knowledge, facts, techniques and rules in a different way.

3 1 1 1 6 (13)

18.5%

4. Analyzing & Evaluating: Examine

and break information into parts by

identifying motives or causes. Make

inferences and evidence to support

generalizations

Present and defend opinions by

making judgment aboutinformation,

validity of ideas or quality of work

based on a set of criteria.

2

2

1

1

6 (14)

20%

5. Creating: Compile information

together in a different wayby

combining elements in a new pattern

or proposing alternative solutions

1 2 1

-

4 (8)

11.5%

TOTAL 15x1=15 7x2=14 7x3=21 4x5=15 70

2) Practical :30marks Duration : 3 hours