Suppl Table 1

Suppl.Tab.1. Homogeneity comparison of amino acid sequences of selective Bacterial gene products with the reserved TM1-H5-TM2 sequence of Kir6.1

NCBI Ref.Sequence

No.Possible function Bacterial species

Percent Identity

(%)

Gene name

NP_669853 Putative transport protein Yersinia pestis KIM 10.31NP_669263 Putative transport protein Yersinia pestis KIM 10.22NP_669190 Putative transport protein Yersinia pestis KIM 9.63

NP_489358 K+ channel protein Nostoc sp PCC 7120 9.24

NP_695956 Hypothetical membrane protein with possible transport function

Bifidobacterium longum 9.25

NP_416061 Putative transport protein Escherichia coli K12 8.66 ydfJ

NP_602106 Putative integral membrane transport protein

Corynebacterium glutamicum

8.6

7

NP_669678 Putative transport protein Yersinia pestis KIM8

8.4NP_298715 Ion transporter Xylella fastidiosa 9a5c9

8.4NP_629116 Putative integral membrane transport protein

Streptomyces coelicolor A3(2)10

8.2NP_628015 Putative ion channel membrane protein

Streptomyces coelicolor 11

7.8NP_627203 Putative integral membrane transport protein

Streptomyces coelicolor A3(2)12

7.4NP_643737 Ion transporter Xanthomonas axonopodis137.4NP_534284 K+-transporting ATPase A chain Agrobacterium tumefaciens str. C5814

7.4NP_416051 Putative transport protein Escherichia coli K1215 ydeE

7.0NP_415044 Putative transport protein Escherichia coli K1216 ybbW

7.0NP_642608 Transport protein Xanthomonas axonopodis176.9NP_668944 Putative ion transport protein Yersinia pestis KIM186.4NP_223160 Putative K+ channel protein Helicobacter pylori J99196.4NP_624744 Putative membrane transport protein Streptomyces coelicolor A3(2)20

5.5NP_350262 K+-transporting ATPase B chain Clostridium acetobutylicum21

5.0NP_636095 K+-transporting ATPase B chain Xanthomonas campestris22

8.6

Suppl Table 2

Suppl.Tab.2. Homogeneity comparison of amino acid sequences of selective bacterial gene products with the reserved TM1-H5-TM2 sequence of Kir6.2

NCBI Ref.Sequence

No.Possible function Bacterial species

Percent Identity

(%)

Gene name

NP_629116 Putative integral membrane transport protein

Streptomyces coelicolor A3(2) 10.91

NP_669190 Putative transport protein Yersinia pestis KIM 9.52NP_627203 Putative integral membrane

transport proteinStreptomyces coelicolor A3(2) 9.53

NP_669263 Putative transport protein Yersinia pestis KIM 9.34

NP_416051 Putative transport protein Escherichia coli K12 9.15

NP_602106 Putative integral membrane transport protein

Corynebacterium glutamicum 9.06

ydeE

NP_628015 Putative ion channel membrane protein

Streptomyces coelicolor

8.8

7

NP_415044 Putative transport protein Escherichia coli K128

8.5NP_350262 K+-transporting ATPase B chain Clostridium acetobutylicum9

8.5NP_624744 Putative membrane transport protein Streptomyces coelicolor A3(2)108.3NP_416061 Putative transport protein Escherichia coli K1211

8.3NP_642608 Transport protein Xanthomonas axonopodis12

8.2NP_298715 Ion transporter Xylella fastidiosa 9a5c138.0NP_223160 Putative K+ channel protein Helicobacter pylori J9914

7.8NP_534284 K+-transporting ATPase A chain Agrobacterium tumefaciens str. C5815

7.8NP_643737 Ion transporter Xanthomonas axonopodis16

7.3NP_489358 K+ channel protein Nostoc sp PCC 7120177.2NP_669853 Putative transport protein Yersinia pestis KIM18

7.2NP_669678 Putative transport protein Yersinia pestis KIM197.2NP_668944 Putative ion transport protein Yersinia pestis KIM20

7.0NP_636095 K+-transporting ATPase B chain Xanthomonas campestris21

6.3NP_695956 Hypothetical membrane protein with possible transport function

Bifidobacterium longum22

8.9

ybbW

ydfJ

Suppl Table 3

Suppl.Tab.3. Amino acid sequence of TM1-H5-TM2 region between rvKir6.1/rvKir6.2 and the selective E. coli K12 genes

GeneName TM1 domain

H5-GFG Motifs

TM2 domain

INA MLGCI QNIVGL

ybbW

ydfJ

ydeE

No match 126 INA186* --- LGL7

No match 359 --- MFGC336 IVG43

No match 62 IGA55 --- ---

rvKir6.1 73-101 (28 aa) 132-134 (3 aa) 155-177 (22 aa)

rvKir6.2 72-100 (28 aa) 122-124 (3 aa) 145-167 (22 aa)

*amino acid sequence order