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PREVIOUS PAGE | NEXT PAGE | SEARCH PRINT | VIEW | EXITReliaPrep™ Large Volume gDNA Purification System
System Overview
Protocol Overview
Walk-Away Automation
Robust Implementation
Scalable Purification
Purification Performance
Summary High-throughput gDNA isolation from
3ml-10ml blood
PREVIOUS PAGE | NEXT PAGE | SEARCH PRINT | VIEW | EXITReliaPrep™ Large Volume gDNA Purification System
System Overview
Protocol Overview
Walk-Away Automation
Robust Implementation
Scalable Purification
Purification Performance
Summary
High-throughput gDNA isolation from 3ml-10ml blood
• 96samplesprocessedin≤8hours,automated
• Robust,precipitation-freeprotocol
• PuregDNAreadyfordemandingapplications
1-96samples 8 hours
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PREVIOUS PAGE | NEXT PAGE | SEARCH PRINT | VIEW | EXITReliaPrep™ Large Volume gDNA Purification System
System Overview
Protocol Overview
Walk-Away Automation
Robust Implementation
Scalable Purification
Purification Performance
Summary
ProtocolNo user intervention needed once the instrument is loaded
• Loadupto96samples,addreagents
• Startthemethod
• Returnin8hourstopuregDNA
8525
MABlood volume
detectedLysis Binding to
particlesElute pure
DNACapture and
release washes
8543
TA85
43TA
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PREVIOUS PAGE | NEXT PAGE | SEARCH PRINT | VIEW | EXITReliaPrep™ Large Volume gDNA Purification System
System Overview
Protocol Overview
Walk-Away Automation
Robust Implementation
Scalable Purification
Purification Performance
Summary
Co-design of instrument, accessories and reagents ensures optimal processingCombination of liquid handling and magnetic particle processing
8544
TA85
44TA
Hamilton MICROLAB® Star Plus ReliaPrep™ 32 LV HSM
• Purificationfromupto9610mlbloodsamples
• Sampleprocessingcompleteinlessthan8hours
• Automatedreagentdeliveryanddetectionofreagentlevels
• Nomanualintervention
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PREVIOUS PAGE | NEXT PAGE | SEARCH PRINT | VIEW | EXITReliaPrep™ Large Volume gDNA Purification System
System Overview
Protocol Overview
Walk-Away Automation
Robust Implementation
Scalable Purification
Purification Performance
Summary
The ReliaPrep™ LV 32 HSM combines a heater, shaker and a magnet for 32 samples in 50ml tubes
Sample tubes never need to be moved!• Reduceschancesofinstrumentfailure
• Simplifiessampletracking
• Allowsefficientuseofplasticsandreagent
8546
TA85
46TA
HamiltonAirdisplacementpipettingwith5mlre-rackable,disposabletipsincreasesspeedanddecreasesplasticsusage.
8544
TA85
44TA
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PREVIOUS PAGE | NEXT PAGE | SEARCH PRINT | VIEW | EXITReliaPrep™ Large Volume gDNA Purification System
System Overview
Protocol Overview
Walk-Away Automation
Robust Implementation
Scalable Purification
Purification Performance
Summary
Scalable purification
8545
TA 8545
TA
STAR plus liquid level sensing detects volume of each sample.
ReliaPrep™ purification chemistry is scalable
• 3mlto10mlinputvolume
VENUS one Instrument Control Software scales each individual well
• providesoptimalperformance
• reducesreagentwaste
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PREVIOUS PAGE | NEXT PAGE | SEARCH PRINT | VIEW | EXITReliaPrep™ Large Volume gDNA Purification System
System Overview
Protocol Overview
Walk-Away Automation
Robust Implementation
Scalable Purification
Purification Performance
Summary
Purification Performance
8522
TA
Purity and Yield
8518
MA
120.00
100.00
80.00
60.00
40.00
20.00
0.00
2.221.81.61.41.210.80.60.40.20
ng/ul
A260/A280
A260/A230Yie
ld/µ
l/ml
Absorbance R
atio
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PREVIOUS PAGE | NEXT PAGE | SEARCH PRINT | VIEW | EXITReliaPrep™ Large Volume gDNA Purification System
System Overview
Protocol Overview
Walk-Away Automation
Robust Implementation
Scalable Purification
Purification Performance
Summary
Summary
• Fullyautomated,walk-awaypurificationprotocol
• ScalablepurificationofgDNAfrom3mlto10mlbloodsamples
• Purificationof96samplesinlessthan8hours
• Robustinstrumentationresultinginlowpurificationfailurerates
• EfficientgDNArecoveryfromchallengingsamples
• PuregDNAreadyforstorageordownstreamassays
Promega Corporation•2800WoodsHollowRoad•Madison,WI53711-5399USA•Telephone608-274-4330•Fax608-277-2601•w w w. p r o m e g a . c o m©2009PromegaCorporation.AllRightsReserved.Pricesandspecificationssubjecttochangewithoutpriornotice.ReliaPrepisatrademarkofPromegaCorporation.MICROLABisaregisteredtrademarkofHamiltonCorporation.
17999 IB GN 10/09Part #GE424
www.promega.com
1-96samples 8 hours
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