Gene Chromosome Start End Function Last exon2 RefSeq number Nucleotide substitution Amino acid substitution rs number human homolog Other mouse strainsCdh201 1 106890959 106890959 stopgain SNV yes NM_011800 c.G2403A p.W801X +

Tlr5 1 184902583 184902584 frameshift substitution no NM_016928 c.18_19delinsT + MSM/Ms, JF1/MsTmem141 2 25476035 25476038 frameshift deletion yes NM_001109993 c.603_606del + MSM/Ms, JF1/Ms

Qsox2 2 26065186 26065186 frameshift deletion yes NM_153559 c.1845delC rs260851851 + NZO/HILtJ, PWK/PhJHc 2 34898728 34898729 frameshift deletion no NM_010406 c.646_647del rs241579076 + A/J, AKR/J,DBA/2J, FVB/NJ,NOD/ShiLtJ

Tdpoz2 3 93455535 93455535 frameshift insertion no NM_001007222 c.1051_1052insAG - MSM/Ms, JF1/MsGm572 4 148045509 148045515 frameshift deletion yes NM_001085505 c.1172_1178del rs258174385 + 129S1/SvImJ, A/J, AKR/J, BALB/cJ, C3H/HeJ, CAST/EiJ, CBA/J,NOD/ShiLtJ, PWK/PhJ, WSB/EiJ

Cyp3a57 5 146138201 146138205 frameshift deletion no NM_001100180 c.957_961del rs242224866 + 129S1/SvImJ, AKR/J, CBA/J, LP/J, NZO/HILtJTry41 6 41253356 41253356 frameshift deletion no NM_011646 c.114delG +Klri21 6 129683766 129683766 stopgain SNV no NM_177155 c.G407A p.W136X -Klra71 6 130169025 130169029 frameshift deletion yes NM_014194 c.693_697del -Ttc231 7 74837804 74837804 frameshift deletion no NM_025905 c.783delT +Olfml1 7 114733919 114733919 frameshift deletion yes NM_172907 c.676delT rs262610229 + 129S1/SvImJ, C3H/HeJ, CAST/EiJ, CBA/J, DBA/J, DBA/2J, LP/J, NOD/ShiLtJ, NZO/HILtJ, PWK/PhJ, WSB/EiJ, , MSM/Ms, JF1/MsFoxr1 9 44243294 44243294 frameshift insertion yes NM_001033469 c.638_639insAA rs215230760 + 129S1/SvImJ, CAST/EiJ, LP/J, PWK/PhJ, , MSM/Ms, JF1/MsCilp 9 65127938 65127938 frameshift deletion yes NM_173385 c.3507delG rs262051220 + 129S1/SvImJ, A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6NJ, CAST/EiJ, CBA/J, DBA/2J, FVB/NJ, LP/J, NOD/ShiLtJ, NZO/HILtJ, PWK/PhJ, SPRET/EiJ, WSB/EiJ, MSM/Ms, JF1/Ms

Slc22a21 11 53764775 53764775 frameshift insertion yes NM_019723 c.1678_1679insC rs231568920 - NZO/HILtJ, PWK/PhJ, MSM/Ms, JF1/MsClec10a1 11 69983716 69983716 stopgain SNV no NM_010796.2 c.C706T p.Q236X +Serpina3i 12 105504794 105504794 frameshift deletion no NM_001199940 c.747delC rs242560633 - 129S1/SvImJ, AKR/J, BALB/cJ, CBA/J, DBA/2J, FVB/NJ, LP/J, NOD/ShiLtJ, WSB/EiJZkscan4 13 21576578 21576578 frameshift insertion yes NM_001039115 c.1416_1417insA rs249591363 + A/J, AKR/J, BALB/cJ, C3H/HeJ, CAST/EiJ, CBA/J, DBA/2J, FVB/NJ, LP/J, NOD/ShiLtJ, WSB/EiJZfp957 14 79613286 79613286 frameshift insertion yes NM_001033215 c.879_880insG rs249051530 - 129S1/SvImJ, A/J, AKR/J, BALB/cJ, C3H/HeJ,CBA/J, FVB/NJ, LP/J, NZO/HILtJ, PWK/PhJ

Timm8a2 14 122434145 122434154 frameshift deletion no NM_001037744 c.237_246del rs237108606 - 129S1/SvImJPdzd21 15 12375345 12375345 stopgain SNV no NM_001081064.1 c.C1054T p.R352X +

Cyp2d11 15 82220453 82220453 frameshift substitution no NM_001104531 c.1156_1156delinsCACCC + 129S1/SvImJ3, A/J3, AKR/J3, BALB/cJ3, C3H/HeJ3, CBA/J3, DBA/2J3, LP/J3, NOD/ShiLtJ3, NZO/HILtJ3

Kcnk7 19 5706988 5706988 frameshift insertion yes NM_010609 c.1004_1005insT + A/J, AKR/J, BALB/cJ, C3H/HeJ, NOD/ShiLtJ, PWK/PhJ, SPRET/EiJ, MSM/Ms, JF1/Ms

1Seven gene mutations, which specifically exist in NC/Nga mice, are shown in bold.2Last exon: variants located in the last exon of the corresponding transcripts.3rs262301704 corresponds to c.1157_1158insCCCA, and mouse strains that carry rs262301704.

Table S1. The stop-gain and frameshift mutations in NC/Nga mouse confirmed by Sanger sequence.

Target1 Control12 Control23

1 MPA Gal 1-3GalNAc (T), GalNAc (Tn) 20283 0 02 BPL Gal 1-3GalNAc (T), terminal GalNAc 19440 0 03 WFA Terminal GalNAc, LacDiNAc 18081 0 04 rSRL Gal 1-3GalNAc (T), GlcNAc 1-3GalNAc, agalacto N-glycan 17794 0 05 rABA Gal 1-3GalNAc (T), GlcNAc 17018 0 06 rXCL Gal 1-3GalNAc (T), GlcNAc 1-3GalNAc, Galacto N-glycan 16918 0 07 rCNL Terminal GalNAc (A, Tn, LacDiNAc) 16900 0 08 Jacalin Gal 1-3GalNAc (T), GalNAc (Tn) 15697 0 09 UDA (GlcNAc)n 15228 0 0

10 rGC2 1-2Fuc (H), GalNAc (A), Gal (B) 15049 0 011 RCA120 Gal 14050 0 012 rPSL1a 2-6Sia 13311 0 013 SBA a, GalNAc (A, Tn, LacDiNAc) 12337 0 014 ABA Gal 1-3GalNAc (T), GlcNAc 11636 0 015 rCGL2 GalNAc 1-3Gal (A), PolyLacNAc 10999 1370 016 PNA Gal 1-3GalNAc (T) 10699 0 017 TJAII 1-2Fuc 10518 0 018 VVA! GalNAc 1-3(4)Gal 10377 0 019 HEA Gal 1-3GalNAc (T) 9936 0 020 rGal9N GalNAc 1-4Gal (A), PolyLacNAc 9093 0 021 rLSLN LacNAc, polylactosamine 8962 0 022 MCA 1-2Fuc 8239 0 023 ACA Gal 1-3GalNAc (T) 8057 0 024 rGal9C PolyLacNAc, Branched LacNAc 7990 0 025 rAAL 1-2Fuc (H), 1-3Fuc (Lex), 1-3Fuc (Lea) 7764 0 026 AAL 1-2Fuc (H), 1-3Fuc (Lex), 1-4Fuc (Lea) 7508 0 027 VVA a, GalNAc (A, Tn, LacDiNAc) 6765 0 028 rACG 2-3Sia 6442 0 029 rBC2LCN Fuc 1-2Gal 1-3GlcNAc (GalNAc) 5909 0 030 HPA GalNAc (A, Tn) 5471 0 031 GNA Man 1-3Man, Man 1-6Man 4458 0 032 NPA Man 1-3Man 3993 0 033 ECA Gal 3871 0 034 DBAI High-man 3793 0 035 rBC2LA Man, High-man 3766 0 036 PVL Sia, GlcNAc 3729 0 037 rRSIIL 1-2Fuc (H), 1-3Fuc (Lex), 1-3Fuc (Lea) 3678 0 038 rMOA Gal (B) 3656 0 039 rGRFT Man 3502 0 040 rAOL 1-2Fuc (H), 1-3Fuc (Lex), 1-3Fuc (Lea) 3194 0 041 CCA Galactosylated N-glycans up to triantenna 3093 0 042 LEL Polylactosamine, (GlcNAc)n 3054 0 043 STL Polylactosamine, (GlcNAc)n 2611 0 044 rPPL a, GalNAc (A, Tn, LacDiNAc) 2579 0 045 rPALa Man5, biantenna 2493 0 046 GSLIA4 GalNAc (A, Tn) 2181 0 047 HHL Man 1-3Man, Man 1-7Man 1731 0 048 rDiscoidin II LacNAc, Gal 1-3GalNAc (T), GalNAc (Tn) 1306 0 049 DSA GlcNAc 1-6Man (Tetraantenna) 1095 0 050 SSA 2-6Sia 1013 0 051 TxLcI Galactosylated N-glycans up to triantenna 973 0 052 rDiscoidin I Gal 737 0 053 rCalsepa Biantenna with bisecting GlcNAc 651 0 054 rGal3C LacNAc, polylactosamine 630 0 055 WGA (GlcNAc)n, polySia 624 0 056 PSA 1-6Fuc up to biantenna 481 0 057 AOL 1-2Fuc (H), 1-3Fuc (Lex), 1-3Fuc (Lea) 309 0 058 LCA 1-6Fuc up to biantenna 228 0 059 rPAIIL Man, 1-2Fuc (H), 1-3Fuc (Lex), 1-4Fuc (Lea) 131 0 060 TJAI 2-6Sia 113 0 061 rBanana Man 1-2Man 1-3(6)Man 10 0 062 ASA Gal 1-4GlcNAc 1-2Man 2 0 062 rCGL3 LacDiNAc 2 0 064 LFA Sia 0 0 064 MAL 2-3Sia 0 0 064 MAH 2-3Sia 0 0 064 ACG 2-3Sia 0 0 064 rGal8N 2-3Sia 0 0 064 SNA 2-6Sia 0 0 064 ADA 2-6Sia, Forssman, A, B 0 0 064 PHAL GlcNAc 1-6Man (Tetraantenna) 0 0 064 rGal7 Type1 LacNAc, chondroitin polymer 0 0 064 rC14 Branched LacNAc 0 0 064 PHAE bisecting GlcNAc 0 0 064 GSLII GlcNAc 1-4Man 0 0 064 PWM (GlcNAc)n 0 0 064 rF17AG GlcNAc 0 0 064 ConA M3, Man 1-2Man 1-3(Man 1-6)Man, GlcNAc 1-2Man 1-3(Man 1-6)Man 0 0 064 Heltuba Man 1-3Man 0 0 064 rHeltuba Man 1-3Man 0 0 064 VVAII Man, agalacto 0 0 064 rOrysata Man 1-3Man, Highman, biantenna 0 0 064 rRSL Man, 1-2Fuc (H), 1-3Fuc (Lex), 1-4Fuc (Lea) 0 0 064 rPTL 1-6Fuc 0 0 064 LTL Lex, Ley 0 0 064 UEAI 1-2Fuc 0 0 064 FLAG-EW29Ch Gal 0 0 064 PTLI GalNAc (A, Tn) 0 0 064 GSLIB4 Gal (B) 0 0 064 EEL Gal (B) 0 0 064 rPAIL a, Gal, GalNAc (Tn) 0 0 064 DBA a, GalNAc (A, Tn, LacDiNAc) 0 0 064 DBAIII Maltose 0 0 064 rMalectin Glc 1-2Glc 0 0 064 CSA Rhamnose, Gal 1-4Gal 0 0 064 FLAG-EW29Ch-E20K 6-sulfo-Gal 0 0 0

Rank Lectin Rough specificity Signal value

1Target: pull-down assay sample from HDM by using Clec10a-Fc.2Control 1: pull-down assay sample from buffer by using Clec10a-Fc.3Control 2: pull-down assay sample from HDM by using human IgG1.Abbreviations: Gal (D-galactose), GalNAc (N-acetyl-galactosamine), GlcNAc (N-acetyl-glucosamine), Fuc (L-fucose), Glc (D-glucose), Sia (Sialic acid), LacNAc (N-acetyl-lactosamine).

Table S2. Lectin microarray analysis of Clec10a-L in HDM.

Clec10a-Fc human IgG1 Clec10a-Fcwith EDTA

1 Gal Gal 1 11934 0 02 Lea Gal 1-3(Fuc 1-4)GlcNAc 1 5532 0 03 Lac Gal 1-4Glc 1 4850 0 04 Core2 Gal 1-3(GlcNAc 1-6)GalNAc 1 4824 0 05 Gal 1-3LN Gal 1-3Gal 1-4GlcNAc 1 4342 0 06 Lex Gal 1-4(Fuc 1-3)GlcNAc 1 2535 0 07 T (Core1) Gal 1-3GalNAc 1 2504 0 08 Lec Gal 1-3GlcNAc 1 2414 0 09 Gal Gal 1 1867 0 0

10 Gal 1-4LN Gal 1-4Gal 1-4GlcNAc 1 1679 0 011 A-di GalNAc 1-3Gal 1 1137 0 012 Tn GalNAc 1 682 0 013 Gal 1-3Lac Gal 1-3Gal 1-4Glc 1 444 0 014 Gal 1-3Gal Gal 1-3Gal 1 144 0 015 di-GalNAc GalNAc 1-3GalNAc 1 65 0 016 Melibiose Gal 1-6Glc 1 45 0 017 Sia3 Neu5Ac 2-8Neu5Ac 2-8Neu5Ac 2 19 0 318 3'SLN Neu5Ac 2-3Gal 1-4GlcNAc 1 7 0 018 LN Gal 1-4GlcNAc 1 7 0 020 GalNAc GalNAc 1 6 0 021 3'SL Neu5Ac 2-3Gal 1-4Glc 1 5 0 022 3'SiaLec Neu5Ac 2-3Gal 1-3GlcNAc 1 4 0 023 Fuc Fuc 1 3 0 023 [3S] Gal (3OSO3)Gal 1 3 0 023 Gal 1-2Gal Gal 1-2Gal 1 3 0 026 H type1 Fuc 1-2Gal 1-3GlcNAc 1 1 0 026 sLex Neu5Ac 2-3Gal 1-4(Fuc 1-3)GlcNAc 1 1 0 026 6'SL Neu5Ac 2-6Gal 1-4Glc 1 1 0 026 STn Neu5Ac 2-6GalNAc 1 1 0 030 Fuc 2Gal Fuc 1-2Gal 1 0 0 030 Fuc 3GlcNAc Fuc 1-3GlcNAc 1 0 0 030 Fuc 4GlcNAc Fuc 1-4GlcNAc 1 0 0 030 H type2 Fuc 1-2Gal 1-4GlcNAc 1 0 0 030 H type3 Fuc 1-2Gal 1-3GalNAc 1 0 0 030 A GalNAc 1-3(Fuc 1-2)Gal 1-4GlcNAc 1 0 0 030 B Gal 1-3(Fuc 1-2)Gal 1-4GlcNAc 1 0 0 030 [3S]Lea (3OSO3)Gal 1-3(Fuc 1-4)GlcNAc 1 0 0 030 Leb Fuc 1-2Gal 1-3(Fuc 1-4)GlcNAc 1 0 0 030 Ley Fuc 1-2Gal 1-4(Fuc 1-3)GlcNAc 1 0 0 030 Neu5Ac Neu5Ac 2 0 0 030 Neu5Gc Neu5Gc 2 0 0 030 Si 2 Neu5Ac 2-8Neu5Ac 2 0 0 030 sLea Neu5Ac 2-3Gal 1-3(Fuc 1-4)GlcNAc 1 0 0 030 [3'S]Lec (3OSO3)Gal 1-3GlcNAc 1 0 0 030 [3'S]LN (3OSO3)Gal 1-4GlcNAc 1 0 0 030 [6S]LN Gal 1-4(6OSO3)GlcNAc 1 0 0 030 [6'S]LN (6OSO3)Gal 1-4GlcNAc 1 0 0 030 LDN GalNAc 1-4GlcNAc 1 0 0 030 G 2 GalNAc 1-4Gal 1-4Glc 1 0 0 030 GlcNAc GlcNAc 1 0 205 030 [6S] GlcNAc (6OSO3)GlcNAc 1 0 318 030 Man Man 1 0 0 030 Man Man 1 0 0 030 [6P]Man (6OPO4)Man 1 0 0 030 Core3 GlcNAc 1-3GalNAc 1 0 0 030 Core4 GlcNAc 1-3(GlcNAc 1-6)GalNAc 1 0 0 030 Forssman disaccharide GalNAc 1-3GalNAc 1 0 0 030 Core6 GlcNAc 1-6GalNAc 1 0 0 030 Core8 Gal 1-3GalNAc 1 0 0 030 [3’S]Core1 (3OSO3)Gal 1-3GalNAc 1 0 0 030 Gal -Core3 Gal 1-4GlcNAc 1-3GalNAc 1 0 0 030 STn (Gc) Neu5Gc 2-6GalNAc 1 0 0 030 ST Neu5Ac 2-3Gal 1-3GalNAc 1 0 0 030 Sia 2-6Core 1 Gal 1-3(Neu5Ac 2-6)GalNAc 1 0 0 030 Glc Glc 1 0 0 030 Glc Glc 1 0 0 030 Maltose Glc 1-4Glc 1 0 0 130 Rha Rhamnose 1 0 0 030 Chitobiose GlcNAc 1-4GlcNAc 1 0 0 030 Negative PAA - 0 0 0

Signal valueRank Glycan Structure

Abbreviations: same as Table S2.Table S3. Glycan microarray analysis of Clec10a-Fc.

Gene Primer sequenceCdh20 Forward TCGGACTCAGAGCAGAGCTT

Reverse CTCTGCTGGGTCCACTCACTTlr5 Forward GCCATTCTTCCTTGAACCAC

Reverse ATGGCCGTGTGGGAGTATAATmem141 Forward GATCAGGGACTCCAAAACCA

Reverse TGCTGAGGTAGGAGGGACTGQsox2 Forward AGACTCAGCCACGTGAACCT

Reverse TCGGGCTCAGACATTTCACTHc Forward TCGTGTTTTTAAATATTTTGCTTCC

Reverse CCCCACCCTCTTCTGGTACTTdpoz2 Forward GGTGGAAGTCAATGGTGGAG

Reverse TTGTCTCTGGGACTCAAAGGAGm572 Forward GTTTCGGCGCTTTTGTTTTA

Reverse CTTCAGAGGCCAGGACAAAGCyp3a57 Forward TGATGTTCTTCTTTGACCTTCC

Reverse TCCCTCTCTGAGTACCATCCATry4 Forward GAGGGCTCCACCTAACAACA

Reverse GTACAGACAGGGCCCATCACKlri2 Forward TGATGAGCACTCATTTCACACA

Reverse TCCCAGTGCCAACAGTTACAKlra7 Forward AAAGTTAAAGAGTTGCCCCTTG

Reverse TGAATTATTGCAGGAAACAAATGTtc23 Forward GAACTGCTCTAACGCTGTGG

Reverse ACAGTGCCATCCAGGGTTCOlfml1 Forward GGGCATTCATGGAAGATAGC

Reverse CATCCACAGCAAGGTCAATGFoxr1 Forward CGCAGTTTCCCCTTCTCAT

Reverse TGGAGGTACAAGGTTCTGTGCCilp Forward AAGAGCAATGTGGGAGTTGC

Reverse AGCATCATGAGGCAGAGACASlc22a21 Forward GCTTGTTTTGCAACTGATGG

Reverse AGCACTGTTGTCGGTCACTGClec10a Forward TGAGGGAGAGGTAACCATGC

Reverse GGGCAAATGTACAGCACACASerpina3i Forward GCTGTCAGGACTCAGCAGTG

Reverse GGTCAGGGAGAATGAACAGGZkscan4 Forward AATCCACACGGGTGAGAAAC

Reverse CAGTGTGTATTGGCCACACCZfp957 Forward TGCAGAGCAAAGTCAAGGTT

Reverse CTTAGCGGCTGCGTTTTTTimm8a2 Forward CATCCACCACATGACAGAGC

Reverse GTCCATTTCCCCACCTACCTPdzd2 Forward ATGCATGCTCGCTTTTTCTT

Reverse GAGGGATGGGGGAAGAGTTACyp2d11 Forward AGGCAGAGTCCAACAGGAAA

Reverse CCTACCTTGGTGACGAGGAAKcnk7 Forward CCCCAGCCTCAGTATCAGAA

Reverse ATTTAGCCCAGAGTCGCTTG

Table S4. The primers for Sanger sequencing validation of stop-gain and frameshift mutations in the NC/Nga genome.

Name Species Origin Source2 Rough specificity1

1 LFA Limax flavus Natural EY Lab. Sia2 WGA Triticum vulgaris Natural EY Lab. (GlcNAc)n, polySia3 PVL Psathyrella velutina Natural Wako Sia, GlcNAc4 MAL Maackia amurensis Natural Seikagaku 2-3Sia5 MAH Maackia amurensis Natural Vector 2-3Sia6 ACG Agrocybe cylindracea Natural JOM 2-3Sia7 rACG Agrocybe cylindracea E.coli AIST 2-3Sia8 rGal8N Homo sapiens E.coli AIST 2-3Sia9 SNA Sambucus nigra Natural Seikagaku 2-6Sia10 SSA Sambucus sieboldiana Natural Vector 2-6Sia11 TJAI Trichosanthes japonica Natural Vector 2-6Sia12 rPSL1a Polyporus squamosus E.coli AIST 2-6Sia13 PHAL Phaseolus vulgaris Natural Seikagaku GlcNAc 1-6Man (Tetraantenna)14 DSA Datura stramonium Natural Seikagaku GlcNAc 1-6Man (Tetraantenna)15 TxLcI Tulipa gesneriana Natural JOM Galactosylated N-glycans up to triantenna16 ECA Erythrina cristagalli Natural Seikagaku Gal17 RCA120 Ricinus communis Natural Vector Gal18 rGal7 Homo sapiens E.coli AIST Type1 LacNAc, chondroitin polymer19 rGal9N Homo sapiens E.coli AIST GalNAc 1-4Gal (A), PolyLacNAc20 rGal9C Homo sapiens E.coli AIST PolyLacNAc, Branched LacNAc21 rC14 Gallus gallus domesticus E.coli AIST Branched LacNAc22 rDiscoidin II Dictyostelium dicodeum E.coli AIST LacNAc, Gal 1-3GalNAc (T), GalNAc (Tn)23 BPL Bauhinia purpurea alba Natural Vector Gal 1-3GlcNAc(GalNAc), / GalNAc24 rCGL2 Homo sapiens E.coli AIST GalNAc 1-3Gal (A), PolyLacNAc25 PHAE Phaseolus vulgaris Natural Vector bisecting GlcNAc26 GSLII Griffonia simplicifolia Natural Vector GlcNAc 1-4Man27 rSRL Sclerotium rolfsii E.coli AIST Core1,3, agalacto N-glycan28 UDA Urtica dioica Natural Vector (GlcNAc)n29 PWM Phytolacca americana Natural Vector (GlcNAc)n30 rF17AG Escherichia coli E.coli AIST GlcNAc31 rGRFT Griffithia sp. E.coli AIST Man32 NPA Narcissus pseudonarcissus Natural Seikagaku Man 1-3Man33 ConA Canavalia ensiformis Natural Vector M3, Man 1-2Man 1-3(Man 1-6)Man, GlcNAc 1-2Man 1-3(Man 1-6)Man34 GNA Galanthus nivalis Natural Vector Man 1-3Man, Man 1-6Man35 HHL Hippeastrum hybrid Natural Vector Man 1-3Man, Man 1-6Man36 ASA Allium sativum Natural JOM Gal 1-4GlcNAc 1-2Man37 DBAI Dioscorea batatas Natural JOM High-man38 CCA Castanea crenata Natural JOM Galactosylated N-glycans up to triantenna39 Heltuba Helianthus t uberosus Natural JOM Man 1-3Man40 rHeltuba Helianthus tuberosus E.coli AIST Man 1-3Man41 ADA Allomyrina dichtoma Natural JOM 2-6Sia, Forssman, A, B42 VVAII Vicia villosa Natural JOM Man, Agalacto43 rOrysata Oryza sativa E.coli AIST Man 1-3Man, Highman, biantenna44 rPALa Phlebodium aureum E.coli AIST Man5, biantenna45 rBanana Musa acuminata E.coli AIST Man 1-2Man 1-3(6)Man46 rCalsepa Calystegia sepium E.coli AIST Biantenna with bisecting GlcNAc47 rRSL Ralstonia solanacearum E.coli AIST Man, 1-2Fuc (H), 1-3Fuc (Lex), 1-4Fuc (Lea)48 rBC2LA Burkholderia cenocepacia E.coli AIST Man, High-man49 AOL Aspergillus oryzae Natural Vector 1-2Fuc (H), 1-3Fuc (Lex), 1-3Fuc (Lea)50 AAL Aleuria aurantia Natural Vector 1-2Fuc (H), 1-3Fuc (Lex), 1-4Fuc (Lea)51 rAAL Aleuria aurantia E.coli AIST 1-2Fuc (H), 1-3Fuc (Lex), 1-3Fuc (Lea)52 rPAIIL Pseudomonas aeruginosa E.coli AIST Man, 1-2Fuc (H), 1-3Fuc (Lex), 1-4Fuc (Lea)53 rRSIIL Ralstonia solanacearum E.coli AIST 1-2Fuc (H), 1-3Fuc (Lex), 1-3Fuc (Lea)54 rPTL Pholiota terrestris E.coli AIST 1-6Fuc55 PSA Pisum sativum Natural Seikagaku 1-6Fuc up to biantenna56 LCA Lens culinaris Natural Vector 1-6Fuc up to biantenna57 rAOL Aspergillus oryzae E.coli AIST 1-2Fuc (H), 1-3Fuc (Lex), 1-3Fuc (Lea)58 rBC2LCN Burkholderia cenocepacia E.coli AIST Fuc 1-2Gal 1-3GlcNAc (GalNAc)59 LTL Lotus tetragonolobus Natural Seikagaku Lex, Ley60 UEAI Ulex europaeus Natural Vector 1-2Fuc61 TJAII Trichosanthes japonica Natural Vector 1-2Fuc62 MCA Momordica charantia Natural JOM 1-2Fuc63 GSLI Griffonia simplicifolia Natural Seikagaku GalNAc (A, Tn), Gal (B)64 PTLI Psophocarpus tetragonolobus Natural Tokyo Kasei GalNAc (A, Tn)65 GSLIA4 Griffonia simplicifolia Natural EY Lab. GalNAc (A, Tn)66 rGC2 Geodia cydonium E.coli AIST 1-2Fuc (H), GalNAc (A), Gal (B)67 GSLIB4 Griffonia simplicifolia Natural Vector Gal (B)68 rMOA Marasmius oreades E.coli AIST Gal (B)69 EEL Euonymus europaeus Natural Vector Gal (B)70 rPAIL Pseudomonas aeruginosa E.coli AIST , Gal, GalNAc (Tn)71 LEL Lycopersicon esculentum Natural Vector Polylactosamine, (GlcNAc)n72 STL Solanum tuberosum Natural Seikagaku Polylactosamine, (GlcNAc)n73 rGal3C Homo sapiens E.coli AIST LacNAc, polylactosamine74 rLSLN Laetiporus sulphureus E.coli AIST LacNAc, polylactosamine75 rCGL3 Coprinopsis cinerea E.coli AIST LacDiNAc76 PNA Arachis hypogaea Natural Vector Gal 1-3GalNAc (T)77 ACA Amaranthus caudatus Natural Vector Gal 1-3GalNAc (T)78 HEA Hericium erinaceum Natural JOM Gal 1-3GalNAc (T)79 ABA Agarics bisporus Natural Vector Gal 1-3GalNAc (T), GlcNAc80 Jacalin Artocarpus integrifolia Natural Seikagaku Gal 1-3GalNAc (T), GalNAc (Tn)81 MPA Maclura pomifera Natural Seikagaku Gal 1-3GalNAc (T), GalNAc (Tn)82 HPA Helix pomatia Natural Seikagaku GalNAc (A, Tn)83 VVA Vicia villosa Natural Vector , GalNAc (A, Tn, LacDiNAc)84 DBA Dolichos biflorus Natural Vector , GalNAc (A, Tn, LacDiNAc)85 SBA Glycine max Natural EY Lab. , GalNAc (A, Tn, LacDiNAc)86 rPPL Pleurocybella porrigens E.coli AIST , GalNAc (A, Tn, LacDiNAc)87 rCNL Clitocybe nebularis E.coli AIST , GalNAc (A, Tn, LacDiNAc)88 rXCL Xerocomus chrysenteron E.coli AIST Core1,3, agalacto N-glycan89 VVA! Vicia villosa Natural JOM GalNAc 1-3(4)Gal90 WFA Wisteria floribunda Natural Vector Terminal GalNAc, LacDiNAc91 rABA Agarics bisporus E.coli AIST Gal 1-3GalNAc (T), GlcNAc92 rDiscoidin I Dictyostelium Discodeum E.coli AIST Gal93 DBAIII Dioscorea batatas Natural JOM Maltose94 rMalectin Homo sapiens E.coli AIST Glc 1-2Glc95 CSA Oncorhynchus keta Natural JOM Rhamnose, Gal 1-4Gal96 FLAG-EW29Ch-E20K Lumbricus terrestris E.coli AIST 6-sulfo-Gal

1Specificity data was obtained by frontal affinity chromatography and glycoconjugate microarray.2Abbreviations in Source: JOM (J-OIL MILLS, INC), Vector (VECTOR LABORATORIES, INC), Seikagaku (SEIKAGAKU CORPORATION), EY (EY LABORATORIES, INC), AIST (National Institute of Advanced Industrial Science and Technology).Other Abbreviations: same as Table S2.

Table S5. Lectins used for lectin microarray.

Trivial name Presentation Glycans Source Cat#Fuc PAA Fuc 1-PAA Glycotech 01-007

Fuc 2Gal PAA Fuc 1-2Gal 1-PAA Glycotech 01-019Fuc 3GlcNAc PAA Fuc 1-3GlcNAc 1-PAA Glycotech 01-024Fuc 4GlcNAc PAA Fuc 1-4GlcNAc 1-PAA Glycotech 01-025

H type1 PAA Fuc 1-2Gal 1-3GlcNAc 1-PAA Glycotech 01-037H type2 PAA Fuc 1-2Gal 1-4GlcNAc 1-PAA Glycotech 08-034H type3 PAA Fuc 1-2Gal 1-3GalNAc 1-PAA Glycotech 08-060

A PAA GalNAc 1-3(Fuc 1-2)Gal 1-4GlcNAc 1-PAA Glycotech 08-091B PAA Gal 1-3(Fuc 1-2)Gal 1-4GlcNAc 1-PAA Glycotech 08-092

Lea PAA Gal 1-3(Fuc 1-4)GlcNAc 1-PAA Glycotech 01-035

[3S]Lea PAA (3OSO3)Gal 1-3(Fuc 1-4)GlcNAc 1-PAA Glycotech 01-040

Leb PAA Fuc 1-2Gal 1-3(Fuc 1-4)GlcNAc 1-PAA Glycotech 08-042

Lex PAA Gal 1-4(Fuc 1-3)GlcNAc 1-PAA Glycotech 01-036

Ley PAA Fuc 1-2Gal 1-4(Fuc 1-3)GlcNAc 1-PAA Glycotech 08-043Neu5Ac PAA Neu5Ac 2-PAA Glycotech 01-012Neu5Gc PAA Neu5Gc 2-PAA Glycotech 01-051Sia2 PAA Neu5Ac 2-8Neu5Ac 2-PAA Glycotech 08-064Sia3 PAA Neu5Ac 2-8Neu5Ac 2-8Neu5Ac 2-PAA Glycotech 01-081

3'SiaLec PAA Neu5Ac 2-3Gal 1-3GlcNAc 1-PAA Glycotech 01-0783'SL PAA Neu5Ac 2-3Gal 1-4Glc 1-PAA Glycotech 01-038

3'SLN PAA Neu5Ac 2-3Gal 1-4GlcNAc 1-PAA Glycotech 01-077

sLea PAA Neu5Ac 2-3Gal 1-3(Fuc 1-4)GlcNAc 1-PAA Glycotech 08-044

sLex PAA Neu5Ac 2-3Gal 1-4(Fuc 1-3)GlcNAc 1-PAA Glycotech 01-0456'SL PAA Neu5Ac 2-6Gal 1-4Glc 1-PAA Glycotech 01-039Gal PAA Gal 1-PAA Glycotech 01-004

[3S] Gal PAA (3OSO3)Gal 1-PAA Glycotech 01-015A-di PAA GalNAc 1-3Gal 1-PAA Glycotech 01-017Lac PAA Gal 1-4Glc 1-PAA Glycotech 01-021

Lec PAA Gal 1-3GlcNAc 1-PAA Glycotech 01-020

[3'S]Lec PAA (3OSO3)Gal 1-3GlcNAc 1-PAA Glycotech 01-062LN PAA Gal 1-4GlcNAc 1-PAA Glycotech 01-022

[3'S]LN PAA (3OSO3)Gal 1-4GlcNAc 1-PAA Glycotech 01-061[6S]LN PAA Gal 1-4(6OSO3)GlcNAc 1-PAA Glycotech 01-066[6'S]LN PAA (6OSO3)Gal 1-4GlcNAc 1-PAA Glycotech 01-068GalNAc PAA GalNAc 1-PAA Glycotech 01-011

di-GalNAc PAA GalNAc 1-3GalNAc 1-PAA Glycotech 01-070LDN PAA GalNAc 1-4GlcNAc 1-PAA Glycotech 01-057GA2 PAA GalNAc 1-4Gal 1-4Glc 1-PAA Glycotech 08-074

GlcNAc PAA GlcNAc 1-PAA Glycotech 01-009[6S] GlcNAc PAA (6OSO3)GlcNAc 1-PAA Glycotech 01-016

Man PAA Man 1-PAA Glycotech 01-005Man PAA Man 1-PAA Glycotech 01-050

[6P]Man PAA (6OPO4)Man 1-PAA Glycotech 01-006Tn PAA GalNAc 1-PAA Glycotech 01-010

Core1 PAA Gal 1-3GalNAc 1-PAA Glycotech 08-023Core2 PAA Gal 1-3(GlcNAc 1-6)GalNAc 1-PAA Glycotech 01-083Core3 PAA GlcNAc 1-3GalNAc 1-PAA Glycotech 01-071Core4 PAA GlcNAc 1-3(GlcNAc 1-6)GalNAc 1-PAA Glycotech 01-089

Forssman PAA GalNAc 1-3GalNAc 1-PAA Glycotech 01-026Core6 PAA GlcNAc 1-6GalNAc 1-PAA Glycotech 01-072Core8 PAA Gal 1-3GalNAc 1-PAA Glycotech 01-028

[3’S]Core1 PAA (3OSO3)Gal 1-3GalNAc 1-PAA Glycotech 08-069Gal -Core3 PAA Gal 1-4GlcNAc 1-3GalNAc 1-PAA Glycotech 01-116

STn PAA Neu5Ac 2-6GalNAc 1-PAA Glycotech 01-059STn (Gc) PAA Neu5Gc 2-6GalNAc 1-PAA Glycotech 01-107

ST PAA Neu5Ac 2-3Gal 1-3GalNAc 1-PAA Glycotech 01-088Sia 2-6Core 1 PAA Gal 1-3(Neu5Ac 2-6)GalNAc 1-PAA Glycotech 01-113

Gal PAA Gal 1-PAA Glycotech 01-003Gal 1-2Gal PAA Gal 1-2Gal 1-PAA Glycotech 01-056Gal 1-3Gal PAA Gal 1-3Gal 1-PAA Glycotech 01-018Gal 1-3Lac PAA Gal 1-3Gal 1-4Glc 1-PAA Glycotech 01-075Gal 1-3LN PAA Gal 1-3Gal 1-4GlcNAc 1-PAA Glycotech 01-079Gal 1-4LN PAA Gal 1-4Gal 1-4GlcNAc 1-PAA Glycotech 01-110Melibiose PAA Gal 1-6Glc 1-PAA Glycotech 01-063

Glc PAA Glc 1-PAA Glycotech 01-001Glc PAA Glc 1-PAA Glycotech 01-002

Maltose PAA Glc 1-4Glc 1-PAA Glycotech 01-054Rha PAA Rhamnose 1-PAA Glycotech 01-008

Chitobiose PAA GlcNAc 1-4GlcNAc 1-PAA Glycotech 08-057Negative PAA PAA - Glycotech 01-000

Abbreviations: same as Table S2.Table S6. Glycans used for glycoconjugate microarray.