*Technical Expertise Addendum

Cell Biology Biochemical Molecular Scientific Instrumentation

Cell culturing (NIH3T3, A7r5, PC12, myoepithelial cells)

Protein Purification (Column, HPLC, FPLC chromatography)

Southern, Northern, and Western Blotting

Zeiss Meta 510 Confocal Microscope Nikon TE 300 Inverted Fluorescent Microscope

Cell Isolation (Percoll, Sucrose Density Gradient) Flow Cytometry

Gel Electrophoresis (SDS-PAGE, isoelectric focusing, 2D gel electrophoresis, DIGE)

Polymerase chain reaction (PCR) and primer design Bioinformatics/Java Programming

Pharmacia (GE Biosciences) FPLC System ABI BioCad Sprint System

EM and light microscopy Zeiss SteREO Nikon TE 300

Western Blotting Antibodies-Monoclonal ELISA

Mobility shift assays ABI 7500 Fast Real-Time PCR System

Immunostaining of cells and tissue sections

ELISA Development Genetic engineering of vectors (cloning and manipulations of plasmids)

Sorval 93SE Ultracentrifuge

Maintenance and genotyping transgenic mice

Subcloning (pGEX/pET vectors) and purification of fusion protein (GST & His tagged proteins)

Isolation of RNA and assays to measure mRNA (real time PCR and RNase Protection Assay)

ABI Prism 310 Genetic DNA sequencer

Confocal Microscopy Zeiss 510 LSM Leica TCS SP5

NMR

Real Time PCR (qPCR) TaqMan and Sybr Green

7900 ABI HT Real Time PCR Machine

Histology

Proteomics

Adenoviral expression of genes and shRNA RNAi technology (Dharmacon and Isis Pharma)

BioRad, GE, and Hoeffer Gel Electrophoresis Systems

Immunohistochemistry Cell Based Assays

Varian Spectrophotometer

Genomics Mouse Models of Disease Transgenic Mice (Jax Labs and Taconic)

VersaMax ELISA plate readers (Molecular Probes)