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TECHNOTHROMBIN® TGA
Fluorogenic Thrombin Generation Assay
For Research Use Only
ML-00-00110 Rev01
FOR RESEARCH USE ONLY
QUANTITATIVE MEASUREMENT OF THROMBIN GENERATION
Time
Inactivation phase
Peak Thrombin
Lag Phase
Slo
pe
TEST PRINCIPLE For Research Use Only
The use of TECHNOTHROMBIN® TGA for such
diverse applications is made possible because
TECHNOTHROMBIN® TGA measures the whole kinet-
ics of thrombin generation not only during the initiation
phase of thrombin formation with the end point fibrin
formation, but also during the phase of down regula-
tion of thrombin formation and inactivation of the
formed thrombin. TECHNOTHROMBIN® TGA is there-
fore an universal tool to be used in the research of the hemostatic system.
Küvette
LED
Photodiode
Measuring principal of TGA modules
The new automated analyser CEVERON® alpha with a TGA module is equipped with a fluorescence
measurement module and thus suitable for assays such as TECHNOTHROMBIN® TGA. With
CEVERON® alpha, standard clotting tests and thrombin generation assays can be performed.
Thrombin Generation is measured with a special adapted TGA fluorimetric module which is placed over the cuvette rotor. With an UV emitter (360 nm) placed in the module, thrombin generation can then be measured.
Alternatively, this assay can be performed manually and measured using a flu-orescence reader. Applications and eva-luation software are available for the most established readers.
Th
rom
bin
TECHNOTHROMBIN® TGA is a Thrombin Generation Assay (TGA) based on monitoring the formation
of thrombin by means of a fluorogenic substrate upon activation of the coagulation cascade by tissue
factor. This assay can be used to research thrombin generation in bleeding and clotting studies.
ML-00-00110 Rev01
FOR RESEARCH USE ONLY
Application dependent reconstitution of thrombin generation potential in Factor VIII inhibitor plasma after addition of FEIBA
(Factor Eight Inhibitor Bypassing Activity)or rFVIIa.
TECHNOTHROMBIN TGA RC LOW/ rVIIa
FVIII INH 3O31000 [58.4 BU/ml]
0
20
40
60
80
100
120
140
0 20 40 60 80 100 120 140
time (min)
nM T
hrom
bin
300E / 300U 150E / 150U 75E / 75U 37,5E / 37,5U
18,8E / 18,8U 9,4E / 9,4U 4,7E / 4,7U 2,3E / 2,3U
1,2E / 1,2U 0,59E / 0,59U 0,29E / 0,29U 0,15E / 0,15U
0,073E /0,73U
TECHNOTHROMBIN® TGA RD / HEPARIN
0,00
100,00
200,00
300,00
400,00
500,00
600,00
0 10 20 30 40 50 60
time [min]n
M T
hro
mb
in
0U/ml RD 8U/ml RD 4U/ml RD 2U/ml RD 1U/ml RD
Application dependent decrease of thrombin
generation potential in plasma after addition of
different heparin or direct Xa inhibitor concentrations.
THROMBIN GENERATION BY MICRO PARTICLES
Thrombin generation induced by micro particles can be measured by the TECHNOTHROMBIN
® TGA assay.
The amount of thrombin generated is dependent on the number of micro particles present in the sample.
FVIII INHIBITOR ANALYSIS HEPARIN ANALYSIS
ML-00-00110 Rev01
FOR RESEARCH USE ONLY
Reagent Purpose
TGA RA - to study the activity of circulating micro particles contained in platelet poor plasma
TGA RB, RC
Low
- research in thrombophilia tendency and FVIII inhibitors
TGA RC High - anticoagulation studies
TGA RD - heparin studies, direct thrombin and Xa inhibitor research
TECHNOTHROMBIN TGA TRIGGER
Technoclone GmbH
Brunner Str. 67 I 1230 Vienna I Austria
Tel: +43 1 86 373-0 I Fax: +43 1 86373-44
[email protected] I www.technoclone.com
TG
A001D
.01
4 0
6/2
013
ADDITIONAL AVAILABLE PRODUCTS:
Modular reagents
REF 5006205 TECHNOTHROMBIN® TGA RA 5 x 0.5 mL
REF 5006206 TECHNOTHROMBIN® TGA RA 50 x 0.5 mL
REF 5006209 TECHNOTHROMBIN® TGA RB 5 x 0.5 mL
REF 5006210 TECHNOTHROMBIN® TGA RB 50 x 0.5 mL
REF 5006212 TECHNOTHROMBIN® TGA RC Low 5 x 0.5 mL
REF 5006213 TECHNOTHROMBIN® TGA RC Low 50 x 0.5 mL
REF 5006214 TECHNOTHROMBIN® TGA RC High 5 x 0.5 mL
REF 5006216 TECHNOTHROMBIN® TGA RC High 50 x 0.5 mL
REF 5006220 TECHNOTHROMBIN® TGA RD 5 x 1.5 mL
REF 5006222 TECHNOTHROMBIN® TGA RD 50 x 1.5 mL
REF 5006235 TECHNOTHROMBIN® TGA SUB 5 x 1.5 mL
REF 5006230 TECHNOTHROMBIN® TGA SUB 50 x 1.5 mL
Additional Controls
REF 5006320 TECHNOTHROMBIN® TGA Control High 5 x 1 mL
REF 5006330 TECHNOTHROMBIN® TGA Control Low 5 x 1 mL
REF 5006345 TECHNOTHROMBIN® TGA CAL Set 1 Set
For T
GA
Reage
nts
for C
evero
n® a
lph
a s
ee s
epara
te brochure.
REF 5006010 TECHNOTHROMBIN® TGA Kit 3x16T.
ML-00-00110 Rev01
FOR RESEARCH USE ONLY