THE EFFECTIVENESS OF PARACHLOROPHENOL CAMPHOR MENTHOL

AGAINST ORAL Candida albicans¹Ayu Trisna Hayati; ²Emma Rachmawati; ³Ame Suciati

Department of ¹Conservative Dentistry, ¹,³Oral Biology, Faculty of Dentistry Universities Padjadjaran

ABSTRACTIntroduction. Parachlorophenol campher menthol is an antiseptic that usually is utilized for endodontic treatment. Nowaday, this antiseptic is still beneficial although there are some new products are used as root canal antiseptic. The study was conducted to asses the inhibition zone and exposure time of this antiseptics. Method. A diffusion disk test and drug dilution test was applied to examine its activity against Candida albicans isolated from oral cavity. Zones of inhibitions were measured in mm after 48 hours of incubation at room temperature and exposures times could be determined by subculturing 0,1 ml Mc Farland standared of Candida albicans suspension in 1 ml antiseptic everyday until the Candida albicans was not growing any longer. Result. The avarage of parachlorophenol campher menthol's inhibition zone is 11 mm and its exposure time is 1,5 days. Conclussion. The data demonstrate that antiseptic of parachlorophenol campher menthol have antifungal effect against Candida albicans as weel as generally synthetic antifungal.

Keyword: Parachlorophenol camphor menthol, Candida albicans

INTRODUCTIONAt present, parachlorophenol camphor menthol (ChKM) is an antiseptic that is still very common used by the dentists in hospitals, health centres (puskesmas), clinics, and privates practices. Antiseptics of ChKM has other pharmacologically advantages as Weine (1985) stated that this antiseptics had a great antifungal effect. There were many researches was conducted to study the effectiveness of ChKM against some fungi, one of them was carried out to examine its effect against Candida albicans through dilution system. The study exhibited antifungal effect of ChKM was great that showed it's activity is 3 to 5 days in inhibiting almost all of any speciesof fungi (Ingle, 1985). So, it was prooved that ChKM has a significant effect against fungi, presumtively it would affect Candida albicans as well. However, at present there has no a test to determine inhibition zone and exposure time of ChKM against Candida albicans which is determined by using disc test.

MATERIALS AND METHODThe study used oral isolates of Candida albicans as a sample and its number was 30 samples. The speciment was taken from oral cavity by swabing oral mucosa that was subsequently cultured in Saburoud Glucose Agar (SGA) and incubated for 48 hours at room temperature. As the colonis appeared, it was examined by gram and biochemistry testing and Candida albicans might give the results to sugar test as glucose positive, sacharose positive, maltose negative, and lactose negative. Candida albicans used as a control was Candida albican of ATCC 10.231. A suspension from strain of Candida albicans was prepared in a sterile physiological solution of 0.5 Mc Farland density. The minimum inhibitory concentration (MIC) used a dilution technique was that a serial dilution with polyethylene glycol. The technique to measure the zone of inhibition used was Shadomiy's technique by utilizing paper disc of 6 mm in diameter and 1 mm in width. The exposure time was obtained by adding 0,1 ml of Candida albicans suspension to 1 ml of the studied antiseptic in their MIC inside the tests tubes. The mixture was incubated at room temperature for 1, 2, 3, 4, 5 days and were inoculated in SGA respectively every 24 hours. The treatments were conducted in 30 of replication, and the datas collected were tabulated.

RESULT

Table 1. The Data's Distribution of Inhibition Zone around ChKM Paper's Disc against Candida albicans

Inhibition zone(mm)

Number of Datas

8 2

8,5 3

9 0

9,5 2

10 6

10,5 2

11 6

11,5 3

12 2

12,5 0

13 2

24 1

Table 2. The Avarage of Inhibition Zone Around ChKM Paper's disc against Candida albicans

Treatment MedianThe avarage of inhibition

zone

ChKM 10,8 11

Table 3. The Data's distribution of Exposure Time and ChKM against Candida albicans

Exposure time(days)

Number of Datas

1 21

2 4

3 4

4 1

5 0

Table 4. The Avarage of Exposure Time of ChKM against Candida albicans

Treatment MedianThe avarage of exposure

time

ChKm 1,0 1,5

CONCLUSIONBased on the result above, it is concluded that antiseptic of ChKM has a great antifungal towards Candida albicans with inhibition zone of 11 mm in avarage and time taken by ChKM in this study to combat the Candida albicans is 1,5 days in avarage.

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