thesis presentation10

7/31/2019 THESIS Presentation10

1/34

DETERMINATION OF LOW DENSITYLIPOPROTEIN (LDL) LOWERING

ACTIVITY OF FORMULATED

CAPSULE FROM RED YEAST(Monascus purpureus) RICE INHYPERLIPIDEMIC RABBITS

Alcarde,Gerallene Mae F.

Sinoy,Alejli Anne C.


2/34

CHAPTER 1

INTRODUCTION


3/34

The Problem and Its setting

WHO STATISTICS

30 % of all global deaths is due to

CARDIOVASCULAR

DISEASES


4/34

DEPARTMENT OF HEALTH

TOP LEADING CAUSE OF MORTALITY


5/34


6/34


7/34


8/34

Monacolin K

LACTONE FORM HYDROXY ACID


9/34

Dr. Dulce Florespresented a studyabout red yeastrice


10/34

STATEMENT OF THE PROBLEM

1. What is the Approximate Effective Dose (AED)of the Red Yeast Rice formulated capsule inrabbits?

2. What is the Effective Dose (ED50) of the RedYeast Rice formulated capsules inhyperlipidemic rabbits?

3. Is there a significant difference in the LDL levelof rabbits when treated with formulatedcapsule of Red yeast rice, Lovastatin Tablet andPlacebo capsule?


11/34

NULL HYPOTHESIS

There is no significant difference on the LDLlevels of the hyperlipidemic rabbits treatedwith formulated capsule of Red yeast ricecompared with Lovastatin tablet and Placebocapsule .


12/34

THEORETICAL FRAMEWORK

Red Yeast rice may lower totalcholesterol by 13-26% and LDL by 21-

33%-Wang et al (1997), Heber et al

(1999), Qin et al (1999), Rippe et al (1999) , Keithley et al (2002)


13/34

RYR and therapeutic lifestyle change decreaseLDL cholesterol level without increasing CPK in

pain levels and may be a treatment option fordyslipidemic patients who cannot toleratestatin-theraphy

(Becker , D., Gordon , R., Halbert, S., French , B.,Morris, P., & Rader , D. (2009, June 16). Redyeast rice for dyslipidemia in statin-intolerant

patients: a randomized trial. . US National Library of Medicine )


14/34

RYRsignificantly reduces totalcholesterol, LDL cholesterol andtotal triacylglycerols concentrationscompared with placebo (Heber etal)


15/34

CONCEPTUAL FRAMEWORK

INDEPENDENTVARIABLEFormulated Capsule of

Red Yeast RiceLovastatin Tablet(positive control)

Placebo( negative control)

DEPENDENTVARIABLE

LDL level (mmol/L)in hyperlipidemicrabbits


16/34


17/34


18/34

To theResearchers

Prove that red yeast rice can

lower the LDL in hyperlipidemicrabbits


19/34

Mortalitycases of CVDS


20/34

Emphasize the effortsmade by the Food andTechnology Department toprovide cost-effectiveproducts for poorhyperlipidemic Filipinos


21/34

To the Philippine Pharmaceutical Society

Help in the production andmarketing of cost-effective drug for hyperlipidemic patients especiallythe poor .


22/34

SCOPE AND DELIMITATION


23/34

The coverage of the study will focus on the

determination of LDL lowering activity levels of theRed Yeast rice and determination of its AED and

ED50 in treating hyperlipidemic rabbits


24/34

Red Yeast Rice : UP Mindanao Food Tech Dept. Lovastatin : commercially available

Experimentation : University of the ImmaculateConception Test Animals (Rabbits) : Rabbit Breeding Farm

in Tugbok District


25/34

PARAMETERS IN MEASURING LDL

Monitoring of :1. Mortality2. body weight

3. food consumption4. water intake5. measurement of LDL levels


26/34

Definition of Terms


27/34

APPROXIMATE EFFECTIVE DOSE.

Approximate Effective Dose is the

estimated dose of a drug to produce adesired response.


28/34

CHOLESTEROL Cholesterol is a sterol and a lipid found in

the cell membranes of all body tissues.


29/34

Effective Dose (ED50) Effective dose is the dose of a drug

predicted to produce a characteristic effectin 50 % of the subjects to whom the doseis given.


30/34

Hyperlipidemia Hyperlipidemia is an increase in the

amount of fat (such as cholesterol andtriglycerides) in the blood. Theseincreases can lead to heart disease andpancreatitis.


31/34

Lipid Profile Lipid Profile is a group of test that

indicates your risk of heart disease. Thelipid profile tests levels of cholesterol, HDLcholesterol, LDL cholesterol andtriglycerides .


32/34

Lovastatin Lovastatin is a widely used statin drug, a

cholesterol lowering agent; anindependent variable used as a positivecontrol in the study.


33/34

LDL (Low Density Lipoprotein)

Low Density Lipoprotein refers to a classand range of lipoprotein particles, varyingin their size (18-25 nm in diameter) andcontents, which carry cholesterol in theblood and around the body, for use bycells.


34/34

Placebo Placebo is an independent variable which

serves as negative control.

thesis presentation10

Documents