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TISSUE REGENERATION in the Classroom!Author(s): Lama Hassoun, Whitney Hable, Tracie Payne-FerreiraSource: The American Biology Teacher, 70(9):546-549. 2008.Published By: National Association of Biology TeachersDOI: http://dx.doi.org/10.1662/0002-7685-70.9.546URL: http://www.bioone.org/doi/full/10.1662/0002-7685-70.9.546

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546 THE AMERICAN BIOLOGY TEACHER, VOLUME 70, NO. 9, NOVEMBER/DECEMBER 2008

For information on adapting this exercise for pre-college students, visit www.nabt.org/Sites/S1/File/pdf/070-09-0044.pdf.

Figure 1. Amputation procedure.

TISSUE REGENERATION 547

Figure 2. A. Wild-type fish on Day 1 B. Typical fin amputation C. Day 7: Post amputation with small plastic ruler measures

Time (Weeks)

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WT 0.0625 g nocodazole 0.09375 g nocodazole

Figure 3. Graph illustrating the rate of regeneration in nocodazole treated and untreated fish over a two-week time period. A more minimal dose of 0.0625 g/ml allows for some tail regen-eration while the higher dose of 0.09375 completely blocks regeneration.

548 THE AMERICAN BIOLOGY TEACHER, VOLUME 70, NO. 9, NOVEMBER/DECEMBER 2008

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