tma in daily pathology use - jojanneke renes€¦ · tma in daily pathology use scientific...
TRANSCRIPT
TMA in daily pathology use
Scientific symposium: New Ways in Cancer Mangement
Hamburg
Jojanneke Renes
24-02-2017
Tissue Microarray (TMA)
TMA pros and cons
Pros Con
Lower costs Tissue heterogeneity
Reducing time
Less patient sample needed
IHC standardization
TMA GrandMaster
- World’s highest capacity: 72 blocks
• 60 donor blocks
• 12 recipient blocks at the same time
4 core diameters
• 0.6 mm max. 558 cores
• 1 mm max. 286 cores
• 1.5 mm max. 135 cores
• 2 mm max. 84 cores
TMA 1.5 and 0.6 mm diameter
85 cores 149 cores
GM TMA software
Prepare HE slides from each donor
block
Scan slides
Mark locations of the cores in
the scanned
slides
TMA production
TMA in stove (56˚C)
Cut sections
from recipient block for
IHC
TMA workflow
Working Mechanism of the TMA GrandMaster
“Old’’ TMA system
Alphelys Westburg
Where do we use the TMA GM for in pathology?
TMA requests
• Research requests
• Control blocks for diagnostics
TMA block
• Tissue
• Diameter
• Pattern
• Donor tissue in 2 – 3 fold
• Reference tissue
Research
• Produce tumour or organ specific TMA blocks
• Protein, DNA and RNA targets• Identify new diagnostic and prognostic markers
Diagnostics
• Positive and negative control
• Quality control
CERB control TMA
ER/PR+
Breast
CERB 3+
Breast
Summary
• TMA brings major economies in time and
costs of slide preparation and staining.
• Quality control for IHC and FISH.
• TMA is a very useful tool in pathology!
Many thanks!
• 6 PCR cassettes
• 10 PCR tubes / cassette
• use of cleaning block to avoid cross contamination
• extracted FFPE tissue samples are ready for DNA extraction
and PCR analysis with commercially available kits
PCR extraction