Today: DNA Extraction!

Shireen Rudina

Procedure1. Mash up your fruit with mortar and pestle, it

must be completely mashed before #2.2. Filter the solution into a beaker, and then

transfer to a test tube.3. Centrifuge for 10 minutes4. Make a solution with 10 mL of detergent and 4 g

of salt.5. Add this solution to your test tube, and swirl to

mix. Incubate for 10 minutes.6. Add COLD ethanol to the test tube and watch

your chromatin form!

Centrifuge must be balanced

What are some of the barriers we need to break to extract DNA?

1. Cell wall

2. Ribosome

3. Golgi complex

4. Nuclear membrane

5. Cell membrane

Which of these objects does NOT have DNA?

1. Fish

2. Rock

3. Flower

4. Bacteria (prokaryotic)

Humans=30,000 genes

3,000 genes

20,000