TRACE EVIDENCES

Dr PRIYAL JAIN

SR Deptt. Of Forensic Medicine

Blood

Semen

Saliva

Urine

Feacal Matter

Blood

Physical examination

Chemical test / Colour Tests

Microchemical test

Spectroscopic examination

Microscopic examination

Chemical tests

Leucomalachite green

Orthotoludine test

Guaiacum test

Benzidine test ( blue colour)

Phenophthaline test / KM Test (pink colour)

peroxidase enzyme causes change in colour

Microchemical test

Teichman`s test / acid hematin test

NaCl + GAA + Stain = brownish black rhombic crystals

Takayama test / hemochromogen test

Reagent + stain = pink feathery hemochromogen

crystals

Positive with old stains, more delicate and reliable,

Can be subjected to spectroscopic examination

Luminol spray test(3-aminophthalhydrazide)

Old stains, minutr traces, non visible stains

When washed with cleaning agents like NH3 or bleach.

Species detection test

Precipitin test

Antiglobulin consumption / hemeagglutination

inhibition

Gel diffusion

Double diffusion in agar gel

Precipitaon electrophoresis

Passive hemeagglutination

Latex test

Semen

Physical examination

Chemical examination

Microscopic examination

Chemical examination

Florence test

dark brown crystals of choline iodide

Barberio test

yellow needle shaped rhombic crystals of spermin

picrate

Acid phosphatase

340-360 Bodonsky / ml ; 2500-3500 Angstrom

Creatine phosphokinase

> 400

Immunological methods

PSA or P30

choline and spermine test