1.Dr Vijay Marakala MBBS, M.D. BIOCHEMISTRY SIMS&RC

2. CONTENT Definition Basic requirements Stages Post translational modification Inhibitors 3. TRANSLATION Process by which ribosomes convert the information carried by mRNA in the form of genetic code to the synthesis of new protein 4. mRNA to be translated tRNAs Ribosomes Energy in the form of ATP and GTP Enzymes and specific factors 5. mRNA to be translated 6. Polycistronic mRNA 7. tRNA 8. Ribosomes 9. Activation of amino acids Initiation Elongation Termination Folding and processing 10. Activation of amino acids 11. Steps involved in initiation Ribosomal dissociation Formation of 43S pre-initiation complex Formation of 48S initiation complex Formation of 80S initiation complex Initiation 12. Initiation 13. Initiation Formation of 43S pre-initiation complex 14. Formation of 48S initiation complex Initiation 15. Initiation Formation of 80S initiation complex 16. Elongation Steps involved in Elongation Binding of aminoacyl tRNA specified by the next coding triplet in the mRNA to the A site Formation of peptide bond Translocation 17. Elongation 18. Termination