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UNIVERSITY OF PENNSYLVANIA - SCHOOL OF VETERINARY MEDICINE

DATE: November 2019

CURRICULUM VITAE

Oliver A Garden BSc BVetMed PhD FRSA FCPP FHEA FRCVS DipACVIM DipECVIM-CA

The Henry and Corinne R Bower Professor of Medicine

Chair of the Department of Clinical Sciences and Advanced Medicine

OFFICE ADDRESS:

University of Pennsylvania

School of Veterinary Medicine

Matthew J Ryan Veterinary Hospital

3900 Delancey Street

Philadelphia, PA 19104

EDUCATION:

King’s College London, UK: Bachelor of Science (First Class), 1990, Pharmacology with Basic

Medical Sciences

Royal Veterinary College, UK: Bachelor of Veterinary Medicine (Distinction in Medicine), 1993:

Veterinary Medicine and Surgery

Royal Veterinary College, UK: Doctor of Philosophy (passed with no corrections), 1998:

Gastrointestinal Immunology

Imperial College London, UK: Certificate of Advanced Studies in Learning and Teaching, 2005:

Further Education / Pedagogy

POSTDOCTORAL TRAINING and FELLOWSHIP APPOINTMENTS:

08.01.93 – 08.01.94: Royal Veterinary College, UK Rotating internship, Department of Small Animal Medicine and Surgery

09.10.94 – 09.10.98: Royal Veterinary College, UK Wellcome Trust Clinical Research Training Scholarship: Combined clinical / PhD program funded by a personal Scholarship, with specialization in gastroenterology and immunology

11.01.98 – 07.01.99: University of South Carolina School of Medicine Wellcome Trust Research Training Fellowship: Postdoctoral program at Palmetto Richland Memorial Hospital, funded by a personal Fellowship

07.12.99 – 07.12.01: Cornell University Hospital for Animals, Ithaca, New York Residency in Small Animal Internal Medicine

08.03.01 – 08.15.04: Imperial College London, UK Wellcome Trust Advanced Fellowship: Postdoctoral program in the Department of Immunology, Division of Medicine, Hammersmith Campus, funded by a personal Fellowship

PRIOR FACULTY APPOINTMENTS:

All at the Royal Veterinary College, UK

08.18.04 – 11.12.11: Senior Lecturer in Internal Medicine

11.13.11 – 08.31.13: Reader in Internal Medicine and Immunology

09.01.13 – 12.02.16: Professor with tenure in Comparative Medicine and Immunology

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HOSPITAL AND UNIVERSITY ADMINISTRATIVE APPOINTMENTS:

Chairman of Departmental Safety Committee, Department of Immunology, Imperial College London, UK (2004 to 2008)

Undergraduate Research Opportunities Program Coordinator, Department of Immunology, Imperial College London (2004 to 2008)

London Technology Network Business Fellow, Department of Immunology, Imperial College London (2005 to 2007)

Member of Genetically Modified Organisms Committee, Royal Veterinary College (RVC), UK (2008 to 2013)

Chairman of Phase 3 Sub-Committee overseeing Intramural Clinical Rotations, Didactic Clinical Elective modules and Research Project 2, before its disbanding; RVC (2009 to 2011)

Member of Learning, Teaching & Assessment and Course Management Committees, RVC (2009 to 2014)

Director of Research Project 2 (Final Year Student Research Project), RVC (2009 to 12/2016)

Director of Elective Teaching Program, RVC (2009 to 2015)

Member of Quality Assurance and Enhancement Strategy Working Group, RVC (2011 to 2014)

Academic Lead of the Oncology Special Interest Group (team of ~70 academics, including postdoctoral fellows and PhD students, with an interest in oncology), RVC (2012 to 12/2016)

RESEARCH and/or CLINICAL INTERESTS:

My research focuses on mechanisms of peripheral tolerance in health and disease, spanning the mouse,

rat and dog as model species of both autoimmune disease and cancer. My work has interrogated the role

of regulatory T cells and more recently myeloid-derived suppressor cells in this context.

SPECIALTY CERTIFICATION:

Royal College of Veterinary Surgeons (UK) Certificate in Small Animal Internal Medicine (September 1996)

Diplomate of the American College of Veterinary Internal Medicine (Small Animal Internal Medicine) (July 2002)

Diplomate of the European College of Veterinary Internal Medicine – Companion Animals (May 2003)

Royal College of Veterinary Surgeons (UK) Specialist in Small Animal Internal Medicine (February 2016)

LICENSURE:

07.27.93: Membership of the Royal College of Veterinary Surgeons (UK)

08.01.05: Fellowship of The Higher Education Academy (UK)

09.23.16: Fellowship of the Royal College of Veterinary Surgeons (UK)

AWARDS AND HONORS:

Undergraduate awards (BSc and BVetMed): Leaper Science Prize (1987), Frank Drury Anatomy Prize (1989), Tompson First Prize (1992), Centenary First Prize (1992)

British Society for Immunology (BSI) Best Poster Prize (of >400 posters on display), Annual Congress, December 2-5, 2003, Harrogate: Monk, CR, Spachidou, M, Rovis, F, Lechler, RI and Garden, OA (2003) MRL/Mp CD4+CD25- T cells resist suppression by CD25+ Tregs in vitro: a defect of effector cells in lupus? Immunology 110 (S1): 81

ProImmune Ltd Poster Prize, BSI Annual Congress, December 2-5, 2003, Harrogate: Rovis, F, Lee, B, Lechler, RI and Garden, OA (2003) Murine CD4+CD25+ Tregs: towards the identification of molecular markers. Immunology 110 (S1): 119

Nature Immunology Poster Prize, The Third International Conference on Regulatory T Cells and Helper T Cell Subsets and Clinical Applications in Human Diseases, October 13-16, 2012,

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Shanghai, China: Pinheiro, D, Pinney, JW and Garden, OA. A phylogenetic examination of regulatory networks within the Animalia Kingdom.

The Kennel Club International Canine Health Award for research into canine health and diseases: May 2017

MEMBERSHIP in LEARNED / PROFESSIONAL SOCIETIES:

Member and then Fellow, Royal College of Veterinary Surgeons: July 1993 to date

Member and then Fellow, Royal Society of Biology: July 1990 to date

Member, British Society for Immunology: August 2001 to date

Member, Comparative Gastroenterology Society: June 2007 to date

Member, European Society of Veterinary Oncology: February 2014 to date

Fellow, The Higher Education Academy: August 2004 to date

Fellow, The Royal Society of Arts, Manufactures and Commerce: January 2016 to date

Fellow, The College of Physicians of Philadelphia: March 2019 to date

PROFESSIONAL ASSOCIATIONS:

Visiting Professor, Division of Immunology and Inflammation, Department of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 ONN, UK (2002 to 2018)

Visiting Professor, Centre for Haemato-Oncology, Barts Cancer Institute, School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, London, EC1M 6BQ, UK (2013 to 2018)

Adjunct Professor, Royal Veterinary College, University of London, NW1 0TU, UK (2016 to present)

NATIONAL / INTERNATIONAL COMMITTEES AND ACTIVITIES:

Reviewer for the Journal of Veterinary Internal Medicine, Veterinary Clinical Pathology, Veterinary Research, In Practice, Immunology, Clinical and Experimental Immunology, Arthritis Research and Therapy, American Journal of Transplantation (2002 to present)

Grant reviewer for Biological and Biotechnology Science Research Council, Wellcome Trust, Medical Research Council

Member of Steering Committee, Comparative Gastroenterology Society Biopsy Archive Project (2008 to 2015)

Member and Angoff Study Participant, ACVIM Examination Rating Committee (2010 to 2014)

Member of European Canine Lymphoma Network initiative (2013 to present)

Chairman, European College of Veterinary Internal Medicine Clinical Studies Grant Fund (2016 to present)

CLINICAL SERVICE at Queen Mother Hospital for Animals (Royal Veterinary College):

Title of

service

Years Number of

Months on

Clinics/year

Weeks/Month

on Clinics

Number of

Days on

clinics per

Week

Hours per week

of direct student

teaching contact

while on clinics

Small

Animal

Internal Medicine

12 3 4 5 (7 when on

weekend duty, which

amounts to 8 weekends per year)

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In my current position as the Chair of the Department, I undertake 10 weeks of clinical service per year, as

an internist in the Matthew J Ryan Hospital.

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PUBLICATIONS:

Peer-Reviewed Research Papers:

IF = impact factor (when published)

Garden, OA and Tuck, ME (1993) Tendon transposition in the surgical management of a soft tissue sarcoma of the limb. Veterinary Record 133: 621-623 [IF 1.50]

Lamb, CR, Wrigley, RH, Simpson, KW, Forster-van Hijfte, M, Garden, OA, Smyth, JBA, Rutgers, HC and White, RN (1996) Ultrasonographic diagnosis of portal vein thrombosis in four dogs. Veterinary Radiology and Ultrasound 37(2): 121-129 [IF 1.06]

White, RN, Trower, ND, McEvoy, FJ, Garden, OA and Boswood, A (1996) A method for controlling portal pressure after attenuation of intrahepatic portacaval shunts. Veterinary Surgery 25: 407-413 [IF 1.57]

White, RN, Boswood, A, Garden, OA and Hammond, RA (1997) Surgical management of subvalvular aortic stenosis and congenital mitral dysplasia in a golden retriever. Journal of Small Animal Practice 38: 251-255 [IF 0.89]

Polvi, A, Garden, OA, Elwood, CM, Sørensen, SH, Batt, RM, Maki, M and Partanen, J (1997) Canine major histocompatibility complex genes DQA and DQB in Irish setter dogs. Tissue Antigens 49: 236-243 [IF 2.33]

Garden, OA, Rutgers, HC, Sørensen, SH, Daniels, S, Walker, D and Batt, RM (1997) Reference range and repeatability of a combined intestinal permeability and function test in Irish setter dogs. Research in Veterinary Science 63: 257-261 [IF 1.35]

Garden, OA, Polvi, A, Houlston, RS, Maki, M, Batt, RM and Partanen, J (1998) Genetic susceptibility to dietary gluten sensitivity in Irish setter dogs is not linked to the major histocompatibility complex. Tissue Antigens 52: 1-7 [IF 2.33]

Garden, OA, Manners, HK, Sørensen, SH, Rutgers, HC, Daniles, S, Legrand-Defretin, V and Batt, RM (1998) Intestinal permeability of Irish setter puppies challenged with a controlled oral dose of gluten. Research in Veterinary Science 65(1): 23-28 [IF 1.35]

Maxwell, A, Elwood, CM, Garden, OA, Camacho-Hubner, C and Batt, RM (1998) Insulin-like growth factor axis of gluten-sensitive dogs during a gluten challenge. Veterinary Record 143: 68-71 [IF 1.50]

Garden, OA, Elwood, CM, Desport, M, and Batt, RM (1999) In situ hybridization as a technique for the immunological investigation of canine intestine: jejunal expression of IFNγ and IL-10 in Irish setters and beagles. Veterinary Immunology and Immunopathology 70: 1-17 [IF 1.96]

Garden, OA, Sørensen, SH and Batt, RM (1999) Challenge of jejunal explants from gluten-sensitive Irish setter dogs with Frazer's fraction III: an immunopathological study of a potential model for coeliac disease. The European Journal of Comparative Gastroenterology 4(1): 7-16 [IF 0.65]

Garden, OA, Pidduck, H, Lakhani, KH, Walker, D, Wood, JLN and Batt, RM (2000) Inheritance of gluten- sensitive enteropathy in Irish setter dogs. American Journal of Veterinary Research 61(4): 462-468 [IF 1.53]

Rich, IN, Worthington-White, DA, Garden, OA and Musk, P (2000) Apoptosis of leukemic cells accompanies reduction in intracellular pH after targeted inhibition of the Na+/H+ exchanger. Blood 95(4): 1427-1434 [IF 10.56]

Garden, OA, Musk, P, Worthington-White, DA, Dewey, MJ and Rich, IN (2000) Silent polymorphisms within the coding region of human sodium / hydrogen exchanger isoform-1 cDNA in peripheral blood

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mononuclear cells of healthy volunteers and leukemia patients. Cancer Genetics and Cytogenetics 120(1): 37-43 [IF 1.54]

Garden, OA, Reubi, JC, Dykes, NL, Yeager, AE, McDonough, SP and Simpson, KW (2005) Somatostatin receptor imaging in vivo by planar scintigraphy facilitates the diagnosis of canine insulinomas. Journal of Veterinary Internal Medicine 19(2): 168-176 [IF 2.17]

Monk, C, Spachidou, M, Rovis, F, Botto, M, Lechler, RI and Garden, OA (2005) MRL/Mp CD4+CD25- T cells resist suppression by CD4+CD25+ Tregs in vitro: a novel defect of T cell regulation in systemic lupus erythematosus. Arthritis and Rheumatism 52(4): 1180-1184 [IF 7.33]

Monk, CR, Sutton-Smith, M, Dell, A and Garden, OA. (2006) Preparation of CD25+ and CD25- CD4+ T cells for glycomic analysis – a cautionary tale of serum glycoprotein sequestration. Glycobiology 16(6): 11G-13G [IF 3.93]

Garden, OA, Reynolds, PR, Yates, J, Larkman DJ, Marelli-Berg FM, Haskard DO, Edwards AD and George AJT (2006) A rapid method for labelling CD4+ T cells with ultrasmall paramagnetic iron oxide nanoparticles for magnetic resonance imaging that preserves proliferative, regulatory and migratory behaviour in vitro. Journal of Immunological Methods 314(1-2): 123-133 [IF 2.35]

Patton, D*, Garden, OA*, Pearce, WP, Clough, LE, Monk, CR, Leung, E, Rowan, WC, Sancho, S, Walker, LSK, Vanhaesebroeck, B, and Okkenhaug, K (2006) Cutting Edge: The phosphoinositide 3-kinase p110δ is critical for the function of CD4+CD25+Foxp3+ regulatory T cells. Journal of Immunology 177(10): 6598- 6602 [*Dual first authorship; IF 5.65]

Yates, J, Rovis, F, Mitchell, P, Afzali, B, Tsang, J Y-S, Garin, M, Lechler, RI, Lombardi, G and Garden, OA (2007) The maintenance of human CD4+CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. International Immunology 19(6): 785-799 [IF 3.40]

Isaacs, JD, Garden, OA, Kaur, G, Collinge, J, Jackson, GS and Altmann, DM (2008) The cellular prion protein is preferentially expressed by CD4+CD25+ Foxp3+ regulatory T cells. Immunology 125(3): 313-319 [IF 4.08]

Kohlhaas, S*, Garden, OA*, Scudamore, C, Turner, M, Okkenhaug, K and Vigorito, E (2009) Cutting Edge: The Foxp3 target miR-155 contributes to the development of regulatory T cells. Journal of Immunology 182(5): 2578-2582 [*Dual first authorship; IF 5.65]

Kathrani, A, Steiner, JM, Suchodolski, J, Eastwood, J, Syme, H, Garden, OA, Allenspach, K (2009) Elevated canine pancreatic lipase immunoreactivity concentration in dogs with inflammatory bowel disease is associated with a negative outcome. Journal of Small Animal Practice 50(3): 126-132 [IF 1.09]

Ferreira, C, Singh, Y, Furmanski, AL, Wong, FS, Garden, OA and Dyson, J (2009) Non-obese diabetic mice select a low-diversity repertoire of natural regulatory T cells. Proceedings of the National Academy of Sciences (USA) 106(20): 8320-8325 [IF 9.64]

Carlucci, F, Fossati-Jimack, L, Dumitriu, IE, Heidari, Y, Walport, MJ, Szajna, M, Baruah, P, Garden, OA, Cook, HT and Botto, M (2010) Identification and characterisation of a lupus suppressor 129 locus on chromosome 3. Journal of Immunology 184(11): 6256-6265 [IF 5.65]

Allenspach, K, House, A, Smith, K, MnNeill, FM, Hendricks, A, Elson-Riggins, J, Riddle, A, Steiner, JM, Werling, D, Garden, OA, Catchpole, B and Suchodolski, JS (2010) Evaluation of mucosal bacteria and histopathology, clinical disease activity and expression of Toll-like receptors in German shepherd dogs with chronic enteropathies. Veterinary Microbiology 146(3-4): 326-335 [IF 2.87]

Gajanayake, I, Priestnall, SL, Benigni, L, English, K, Summers, B and Garden, OA (2010) Paraneoplastic hypercalcemia in a dog with benign renal angiomyxoma. Journal of Veterinary Diagnostic Investigation 22(5): 775-780 [IF 1.55]

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Singh, Y, Ferreira, C, Chan, AC, Dyson, J and Garden, OA (2010) Restricted TCR-α CDR3 diversity disadvantages natural regulatory T cell development in the B6.2.16 beta chain transgenic mouse. Journal of Immunology 185(6): 3408-3416 [IF 5.65]

Bailey, S, Benigni, L, Eastwood, J, Garden, OA, McMahon, L, Smith, K, Steiner, JM, Suchodolski, JS and Allenspach, K (2010) Comparisons between cats with normal and increased fPLI concentrations in cats diagnosed with inflammatory bowel disease. Journal of Small Animal Practice 51(9): 484-489 [IF 1.09]

Pinheiro, D, Singh, Y, Grant, C, Appleton, RC, Sacchini, F, Walker, KRL, Chadbourne, AH, Palmer, CA, Armitage-Chan, E, Thompson, I, Williamson, L, Cunningham, F and Garden, OA (2011) Phenotypic and functional characterisation of a CD4+CD25highFOXP3high regulatory T cell population in the dog. Immunology 132(1): 111-122 [IF 4.08]

Kathrani, A, Schmitz, S, Priestnall, SL, Smith, KC, Werling, D, Garden, OA and Allenspach, K (2011) CD11c+ cells are significantly decreased in the duodenum, ileum and colon of dogs with inflammatory bowel disease. Journal of Comparative Pathology 145(4): 359-366 [IF 1.73]

Singh, Y, Dyson, J and Garden, OA (2011) Use of SNARF-1 to measure murine T cell proliferation in vitro and its application in a novel regulatory T cell suppression assay. Immunology Letters 140(1-2): 21-29 [IF 2.91]

Schmitz, S, Garden, OA, Werling, D and Allenspach, K (2012) Gene expression of selected signature cytokines of T cell subsets in duodenal tissues of dogs with and without inflammatory bowel disease. Veterinary Immunology and Immunopathology 146(1): 87-91 [IF 1.96]

Soond, DR, Garçon, F, Patton, DT, Rolf, J, Turner, M, Scudamore, C, Garden, OA and Okkenhaug, K (2012) Pten loss in CD4+ T cells enhances their helper function but does not lead to autoimmunity or lymphoma. Journal of Immunology 188(12): 5935-5943 [IF 5.65]

Kidder, D, Richards, HE, Ziltener, HJ, Garden, OA and Crocker, PR (2013) Sialoadhesin ligand expression identifies a subset of CD4+Foxp3- T cells with a distinct activation and glycosylation profile. Journal of Immunology 190(6): 2593-2602. [IF 5.65]

Walker, D, Knuchel-Takano, A, McCutchan, A, Chang, Y-M, Downes, C, Miller, S, Stevens, K, Verheyen, K, Phillips, AD, Miah, S, Turmaine, M, Hibbert, A, Steiner, JM, Suchodolski, JS, Mohan, K, Eastwood, J, Allenspach, K, Smith, K and Garden, OA (2013) A comprehensive pathological survey of duodenal biopsies from dogs with diet-responsive chronic enteropathy. Journal of Veterinary Internal Medicine 27(4): 862-874 [IF 2.17]

Lawrence, J, Chang, YM, Szladovits, B, Davison, LJ and Garden, OA (2013) Breed-specific hematological phenotypes in the dog: a natural resource for the genetic dissection of hematological parameters in a Mammalian species. PLOS ONE 8(11): e81288 [IF 3.73]

Ferreira, C, Palmer, D, Blake, K, Garden, OA and Dyson, J (2014) Reduced regulatory T cell diversity in NOD mice is linked to early events in the thymus. Journal of Immunology 192(9): 4145-4152 [IF 5.65]

Hardy, JI, Hendricks, A, Loeffler, A, Chang, YM, Verheyen, KL, Garden, OA and Bond, R (2014) Food- specific serum IgE and IgG reactivity in dogs with and without skin disease: lack of correlation between laboratories. Veterinary Dermatology 25(5): 447-e70 [IF 1.99]

Anfinsen, KP, Piercy, RJ, Massey, C, Smith, KC, Kenny, PJ and Garden, OA (2014) Severe systemic calciphylaxis in a young cat. Journal of Veterinary Internal Medicine 28(4): 1325-1330 [IF 2.17]

Pinheiro, D, Chang, YM, Bryant, H, Szladovits, B, Dalessandri, T, Davison, LJ, Yallop, E, Mills, E, Leo, C, Lara, A, Stell, A, Polton, G and Garden, OA (2014) Dissecting the regulatory microenvironment of a large animal model of non-Hodgkin lymphoma: evidence of a negative prognostic impact of FOXP3+ T cells in canine B cell lymphoma. PLOS ONE 9(8): e105027 [IF 3.73]

Schmitz, S, Glanemann, B, Garden, OA, Brooks, H, Chang, YM, Werling, D and Allenspach, K (2015) A prospective, randomized, blinded, placebo-controlled pilot study on the effect of Enterococcus faecium on clinical activity and intestinal gene expression in canine food-responsive chronic enteropathy. Journal of Veterinary Internal Medicine 29(2): 533-543 [IF 2.17]

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Swann, JW, Priestnall, SL, Dawson, C, Chang, YM and Garden, OA (2015) Histologic and clinical features of primary and secondary vasculitis: a retrospective study of 42 dogs (2004-2011). Journal of Veterinary Diagnostic Investigation 27(4): 489-496 [IF 1.35]

Lowrie, M, Garden, OA, Hadjivassiliou, M, Harvey, RJ, Sanders, DS, Powell, R and Garosi, L (2015) The clinical and serological effect of a gluten-free diet in Border terriers with epileptoid cramping syndrome. Journal of Veterinary Internal Medicine 29(6): 1564-1568 [IF 2.17]

Singh, Y, Garden, OA, Lang, F and Cobb, BS (2015) MicroRNA-15b/16 enhances the induction of regulatory T cells by regulating the expression of Rictor and mTOR. Journal of Immunology 195(12): 5667-5677 [IF 5.65]

Peters, LM, Glanemann, B, Garden, OA and Szladovits, B (2016) Cytological findings of 140 bile samples from dogs and cats and associated clinical pathological data. Journal of Veterinary Internal Medicine 30(1): 123-131 [IF 2.17]

Carney, K, Chang, Y-M, Wilson, S, Calnan, C, Reddy, PS, Chan, W-Y, Gilmartin, T, Hernandez, G, Schaffer, L, Head, SR, Morley, J, de Mestre, A, Affleck, K and Garden, OA (2016) Regulatory T cell- intrinsic amphiregulin is dispensable for suppressive function. Journal of Allergy and Clinical Immunology 137(6): 1907-1909 [IF 11.5]

Denyer, MP, Pinheiro, DY, Shepherd, AJ* and Garden, OA* (2016) Missed, not missing: phylogenomic evidence for the existence of avian FoxP3. PLOS ONE 11(3): e0150988 [*Dual last and corresponding authorship; IF 3.73]

Chang, Y-M, Hadox, E, Szladovits, B and Garden, OA (2016) Serum biochemical phenotypes in the domestic dog. PLOS ONE 11(2): e0149650 [IF 3.73]

Aguiar, J, Chang, YM and Garden, OA (2016) Complications of percutaneous endoscopic gastrostomy in dogs and cats receiving corticosteroid treatment. Journal of Veterinary Internal Medicine 30(4): 1008-1013 [IF 2.17]

Singh, Y, Garden, OA, Lang, F and Cobb, BS (2016) MicroRNAs regulate T-cell production of interleukin- 9 and identify hypoxia-inducible factor-2α as an important regulator of T helper 9 and regulatory T-cell differentiation. Immunology 149(1): 74-86 [IF 4.08]

Fortuna, L, Relf, J, Chang, YM, Hibbert, A, Martineau, HM* and Garden, OA* (2016) Prevalence of FoxP3+

cells in canine tumours and lymph nodes correlates positively with glucose transporter 1 expression. Journal of Comparative Pathology 155(2-3): 171-180 [*Dual last authorship; IF 1.38]

Giuliano, A, Swift, R, Arthurs, C, Marote, G, Abramo, F, McKay, J, Thomson, C, Beltran, M, Millar, M, Preistnall, S, Dobson, J, Costantino-Casas, F, Petrou, T, McGonnell, IM, Davies, AJ, Weetman, M, Garden, OA, Masters, JR, Thrasivoulou, C and Ahmed, A (2016) Quantitative expression and co- localization of Wnt signaling related proteins in feline squamous cell carcinoma. PLOS ONE 11(8): e0161103 [IF 3.73]

Singh, Y, Garden, OA, Florian, L and Cobb, BS (2016) Retroviral transduction of helper T cells as a genetic approach to study mechanisms controlling their differentiation and function. Journal of Visualized Experiments Nov 4 (117) doi: 10.3791/54698 [IF 1.33]

Lowrie, M, Jacinto, A, Hadjivassiliou, M, Sanders, DS and Garden, OA (2016) A presumptive case of gluten sensitivity in a Border terrier: a multi-system disorder? Veterinary Record 179(22): 573 [IF 1.50]

Swann, JW, Woods, K, Wu, Y, Glanemann, B and Garden, OA (2016) Characterisation of the immunophenotype of dogs with primary immune-mediated haemolytic anaemia, PLOS ONE 11(12): e0168296 [IF 3.73]

Comazzi, S, Avery, PR, Garden, OA, Riondato, F, Rütgen, B, Vernau, W; European Lymphoma Network (2017) European canine lymphoma network consensus recommendations for reporting flow cytometry in canine hematopoietic neoplasms. Cytometry B Clinical Cytometry 92(5): 411-419 [IF 2.40]

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Marconato, L, Polton, G, Sabattini, S, Dacasto, M, Garden, OA, Grant, I, Henrickx, T, Henriques, J, Lubas, G, Morello, E, Stefanello, D, Comazzi, S; European Lymphoma Network (2017) Conformity and controversies in the diagnosis, staging and follow-up evaluation of canine nodal lymphoma: a systematic review of the last 15 years of published literature. Veterinary and Comparative Oncology 15(3): 1029-1040 [IF 2.45]

Muir, C, Priestnall, SL, Hibbert, A, Brown, C, Garden, OA* and Scase, T* (2017) Prevalence of FoxP3+

cells does not correlate with Ki67 expression in canine diffuse large B-cell lymphoma. Journal of

Comparative Pathology 157(1): 15-22 [* Dual last authorship; IF 1.17]

Gibbons, N, Goulart, MR, Chang, Y-M, Efstathiou, K, Purcell, R, Wu, Y, Peters, LM, Turmaine, M,

Szladovits, B and Garden, OA (2017) Phenotypic heterogeneity of peripheral monocytes in healthy dogs.

Veterinary Immunology and Immunopathology 190:26-30 [IF 1.54]

Heaps, LA, Scudder, CJ, Lipscomb, VJ, Steinbach, SM, Priestnall, SL, Martineau, H, Szladovits, B, Fowkes,

RC and Garden, OA (2017) Serum anti-Müllerian hormone concentrations before and after treatment of an

ovarian granulosa cell tumour in a cat. Journal of Feline Medicine and Surgery Open Reports 3(2):

2055116917722701 [IF 1.21]

Davies, O, Szladovits, B, Polton, G, Garden OA, Leo, C and Lara, A (2018) Prognostic significance of clinical presentation, induction and rescue treatment in 42 cases of canine centroblastic diffuse large B-cell multicentric lymphoma in the United Kingdom Veterinary and Comparative Oncology 16(2): 276-287 [IF 2.45]

Pittaway, R, Wu, Y, Szladovits, B, Suarez-Bonnet, A, Scurrell, EJ, Garden, OA, Polton, G and Priestnall,

SL (2018) Diagnosis of anaplastic large-cell lymphoma in a dog using CD30 immunohistochemistry. Journal

of Veterinary Diagnostic Investigation 30(3): 455-458 [IF1.35]

Lowrie, M, Garden, OA, Hadjivassiliou, M, Sanders, DS, Powell, R and Garosi, L. (2018) Characterization of paroxysmal gluten-sensitive dyskinesia in Border terriers using serological markers. Journal of Veterinary Internal Medicine 32(2): 775-781 [IF 2.17]

Crossfield, L, Fortuna, L, Carling, R, Matas Riera, M, Hampel, R, Dawson, C, Yeung, I, Chang, Y-M,

Scurrell, E, Thaung, C, Priestnall, S and Garden, OA (2019) Immunohistochemical characterization of

feline lymphoplasmacytic anterior uveitis. Veterinary Ophthalmology 22(2): 206-212 [IF 1.08]

Garden, OA*, Kidd, L*, Mexas, AM, Chang, YM, Jeffery, U, Blois, S, Fogle, JE, MacNeill, AL, Lubas, G, Birkenheuer, A, Buoncompagni, S, Dandrieux, JRS, Di Loria, A, Fellman, C, Glanemann, B, Goggs, R, Granick, J, LeVine, D, Sharp, CR, Smith-Carr, S, Swann, JW and Szladovits, B (2019) ACVIM consensus statement on the diagnosis of immune-mediated hemolytic anemia in dogs and cats. Journal of Veterinary Internal Medicine 33(2): 313-334 [*Dual first authorship; IF 2.17] Swann, JW*, Garden, OA*, Fellman, CL, Glanemann, B, Goggs, R, LeVine, DN, Mackin, AJ and Whitley, NT (2019) ACVIM consensus statement on the treatment of immune-mediated hemolytic anemia in dogs. Journal of Veterinary Internal Medicine 33(3): 1141-1172 [*Dual first authorship; IF 2.17] Goulart, MR, Hlavaty, SI, Chang, YM, Polton, G, Stell, A, Perry, J, Wu, Y, Sharma, E, Broxholme, J, Lee, AC, Szladovits, B, Turmaine, M, Gribben, J, Xia, D* and Garden, OA* (2019) Phenotypic and transcriptomic characterization of canine myeloid-derived suppressor cells. Scientific Reports 9(1): 3574; doi: 10.1038/s41598-019-40285-3 [*Dual last authorship; IF 4.12] Swann, JW, Szladovits, B, Threlfall, AJ, Garden, OA, Chang, YM, Church, DB and Glanemann, B (2019) Randomised controlled trial of fractionated and unfractionated prednisolone regimens for dogs with immune-mediated haemolytic anaemia. The Veterinary Record 184(25): 771; doi: 10.1136/vr.105104 [IF 1.76] Wu, Y, Chang, YM, Stell, AJ, Priestnall, SL, Sharma, E, Goulart, MR, Gribben, J, Xia, D and Garden, OA (2019) Phenotypic characterisation of regulatory T cells in dogs reveals signature transcripts conserved in humans and mice. Scientific Reports 9(1): 13478; doi: 10.1038/s41598-019-50065-8. [IF 4.12] Hlavaty, SI, Chang, YM, Orth, RP, Goulian, M, Planet, PJ, Thamm, DH, Punt, JA and Garden, OA (2019) Bacterial killing activity of polymorphonuclear myeloid-derived suppressor cells isolated from tumor-bearing dogs. Frontiers in Immunology 10: 2371; doi: 10.3389/fimmu.2019.02371 [IF 6.43]

9

Review Articles (Peer-Reviewed):

Garden, OA (1992) The myxosporea of fish: a review. British Veterinary Journal 148(3): 223-239 [IF 2.32]

Elwood, CM and Garden, OA (1999) Gastrointestinal immunity in health and disease. Veterinary Clinics of North America: Small Animal Medicine and Surgery 29(2): 471-500 [IF 0.84]

Garden, OA (2001) Genetic modelling of intestinal disease: the paradigm of gluten-sensitive enteropathy in the Irish setter dog – a model for human coeliac disease? The European Journal of Comparative Gastroenterology 10: 2-12. [IF 0.65]

Lechler, RI, Garden, OA and Turka, LA (2003) The complementary roles of deletion and regulation in the maintenance of transplantation tolerance. Nature Reviews Immunology 3(2): 147-158 [IF 34.99]

Haslam, SM, Julien, S, Burchell, JM, Monk, CR, Ceroni, A, Garden, OA and Dell, A (2008) Characterizing the glycome of the mammalian immune system. Immunology and Cell Biology 86(7): 564-573 [IF 4.20]

Garden, OA, Pinheiro, DY and Cunningham, F (2010) All creatures great and small: regulatory T cells in mice, humans, dogs and other domestic animal species. International Immunopharmacology 11(5): 576- 588 [IF 2.21] Lewis, DH, Chan, DL, Pinheiro, D, Armitage-Chan, E and Garden, OA (2012) The immunopathology of sepsis: pathogen recognition, systemic inflammation, the compensatory anti-inflammatory response, and regulatory T cells. Journal of Veterinary Internal Medicine 26(3): 457-482 [IF 2.17]

Soond, DR, Slack, EC, Garden, OA, Patton, DT and Okkenhaug, K (2012) Does the PI3K pathway promote or antagonize regulatory T cell development and function? Frontiers in Immunology 3: 244 [IF TBC]

Burr, SP, Dazzi, F and Garden, OA (2013) Mesenchymal stromal cells and regulatory T cells: the Yin and Yang of peripheral tolerance? Immunology and Cell Biology 91(1): 12-18 [IF 4.20]

Swann, JW and Garden, OA (2016) Novel immunotherapies for immune-mediated haemolytic anaemia in dogs and people. The Veterinary Journal 207: 13-19 [IF 1.76] Garden, OA, Volk, SW, Mason, NJ and Perry, JA (2018) Companion animals in comparative oncology: One Medicine in action. The Veterinary Journal 240: 6-13 [IF 1.76] Klotsman, M, Sathyan, G, Anderson, WH, Garden, OA and Shivanand, P (2018) Mycophenolic acid in patients with immune-mediated inflammatory diseases: From humans to dogs. Journal of Veterinary Pharmacology and Therapeutics 42(2: 127-138 [IF 1.44]

Book Chapters and Reviews:

Wheal, HV, Bruce, R, Best, N, Garden, OA and Mitchell, J (1992) Failure of synaptic inhibition and changes in the population of some non-pyramidal cells in a chronic model of temporal lobe epilepsy. In Inhibition and Epilepsy, eds Speckman, E-J and Gutnick, MJ. Munchen: Urban and Schwarzenberg

Garden, OA, Day, MJ, Elwood, CM and Rutgers, HC (1997) Polysystemic immune-mediated disease in the dog and cat. Part I: Pathogenesis and clinicopathological features. Veterinary International 9(2): 3-16

Garden, OA, Day, MJ, Elwood, CM and Rutgers, HC (1997) Polysystemic immune-mediated disease in the dog and cat. Part II: Treatment options and future directions. Veterinary International 9(3): 2-12

Rovis, F, Wait, R, Lee, BBC, Monk, CR, Lechler, RI and Garden, OA (2004) Proteomic characterization of murine CD4+CD25+ regulatory T cells. In Immunology 2004 (ISBN 88-7587-069-1). Bologna: Medimond Srl

Garden, OA (2010) Chapter 185: Immune-mediated diseases and therapies. In Textbook of Veterinary Internal Medicine (Seventh Edition), eds Ettinger, SJ and Feldman, EC. Saunders Elsevier, Philadelphia

Garden, OA (2011) Chapter 3: Gastrointestinal immunology. In Canine and Feline Gastroenterology (First Edition), eds Washabau, R and Day, MJ. Saunders Elsevier, Philadelphia

10

Book in Preparation: Veterinary Clinical Immunology, Wiley Press; anticipated publication 2020 Garden, OA and Kidd, L (eds)

Published Abstracts:

Over 90, which have led to full papers (please see above)

A full bibliography may be found at: https://www.ncbi.nlm.nih.gov/sites/myncbi/10_0zm7XQmSQe4/bibliography/56691607/public/?sort=date&direction=ascending

Citation metrics

H index: 26

i10 index: 50

Total citations: 2,783 (as of November 4th, 2019)

11

GRANT PAGES

All costs in USD, assuming an exchange rate of 1 GBP = 1.3 USD

PAST

Name of Grant & sponsor number

Funding Agency

Period of Grant

Type of Grant (see grant

category below)

Role in Grant and

percent effort

Annual Direct Cost

Annual Indirect

Cost

Additional Comments

Development of a murine T cell expander bead

Invitrogen- Dynal

10.10.03 to

10.09.04

IG Principal Investigator

20%

98,826 98,826

Studies of conventional and regulatory T cells in a mouse model of type 1 diabetes mellitus

Commonwealth Commission

03.03.06 to

03.31.09

O Principal Investigator

15%

32,488 N/A

Evaluation of the zonulin receptor antagonist AT- 1001 in the treatment of canine inflammatory bowel disease

Alba Therapeutics Corporation

02.26.07 to

02.25.09

CT Principal Investigator

15%

36,110 36,110

PI3K signaling in regulatory T cells

BBSRC 06.01.07 to

05.31.10

FG Co- Investigator

10%

170,186 42,546 UK research council project

grant

Characterization of regulatory T cells in healthy and atopic dogs

Biotechnology and Biological

Sciences Research Council

(BBSRC)

07.01.07 to

09.30.10

TG Principal Investigator

15%

36,227 9,057 This was a Collaborative

Award in Science and Engineering (CASE) with support from

Novartis Animal Health as the

industrial partner

Investigating the role of regulatory T cells in sepsis- induced immunoparalysis in dogs with pneumonia

European College of Veterinary

Internal Medicine

07.01.07 to

09.30.09

PG Principal Investigator

5%

4,076 N/A

Role of regulatory T cells in the immune response

Italian Ministry of Health

09.01.07 to

08.31.09

FG Co- Investigator

3%

N/A 2,733 My role was in the design and interpretation of

12

of dogs infected with Leishmania spp.

experiments performed in Italy: only my time was being paid for in UK

Glycomic analysis of CD4+CD25+

regulatory T cells

Mizutani Foundation for Glycoscience

09.17.07 to

08.18.08

PG Principal Investigator

15%

24,930 2,770

Investigation of the role of regulatory T cells in the immunoparalysis evoked by naturally occurring sepsis in dogs

American College of Veterinary Emergency and Critical

Care

10.01.07 to

09.30.09

PG Co- Investigator

10%

3,201 N/A

Genetic control of TCR diversity within the natural regulatory T cell lineage

BBSRC 06.01.08 to

05.31.11

FG Co- Investigator

15%

53,671 13,418

Characterization of canine CD4+CD25+

regulatory T cells

University of London Central Research Fund

08.01.09 to

09.01.10

PG Principal Investigator

3%

3,900 N/A

Amphiregulin – a novel regulatory T cell effector molecule?

BBSRC 10.01.10 to

09.30.14

FG Principal Investigator

15%

42,937 10,743 A CASE Award with

GlaxoSmithKline as the industrial

partner The role of PI3K in regulatory T cell function

Wellcome Trust

10.01.10 to

09.30.16

F Principal Investigator

15%

94,310 23,578 This was a Veterinary Integrated

Fellowship held by Dr Elizabeth Slack, based at

the Royal Veterinary

College (grant holder) and the

Babraham Institute,

Cambridge

Investigating the pathogenic role of regulatory T cells in canine B cell lymphoma

Petplan Charitable

Trust

12.15.10 to

12.14.12

PG Principal Investigator

15%

61,460 10,846

To split or not to split – that is the question!

PetSavers 06.01.13 to

06.01.15

PG Co- Investigator

3%

5,201 N/A

Liquid biopsies of canine cancer: a pilot study of epigenetic modifications of tumour DNA in blood-borne

Belgian Volition 10.01.14 to

10.01.16

IG Principal Investigator

5%

9,750 9.750

13

nucleosomes assayed in dogs with round cell, mesenchymal and epithelial cancers

Immune privilege in canine B cell lymphoma: a ménage a trois between neoplastic B cells, regulatory T cells and myeloid- derived suppressor cells?

Petplan Charitable

Trust

07.13.14 to

07.14.17

PG Principal Investigator

15%

67,319 11,880 This grant employed a postdoctoral

Fellow in Cancer

Immunology

Defining the immunophenotype of dogs with immune-mediated hemolytic anemia

Kennel Club 08.01.14 to

08.01.16

PG Co- Investigator

10%

7,937 N/A

Hypoxia, regulatory cells and the DNA damage response in cancer: the dog model

PetSavers 10.01.14 to

01.01.16

PG Principal Investigator

5%

5,958 N/A

The comparative cytological and immune-regulatory phenotypes of canine multicentric and human non- Hodgkin lymphoma

Royal Veterinary College /

Queen Mary University of

London intramural

grant

10.21.13 to

09.01.18

PG

Principal Investigator

10%

60,783 6,754 This grant is supporting a

PhD studentship

The cellular and molecular phenotype of regulatory cells in the tumor micro-environment of canine multicentric B cell lymphoma

Animal Care Trust

06.01.16 to

09.01.18

O Principal Investigator

10%

58,500 N/A This was a private

philanthropic donation to the RVC’s charity,

the Animal Care Trust, to support

my research

*For Type of Grant, use code in bold from the following menu:

R01 NIH R01

PP NIH Program Project, Center or Core Grants

FG Federal Grants - Other (including other individual NIH grants and grants from VA, NSF, Dept. of Energy, etc.) F Fellowship

CT Clinical Trials

TG Training Grants IG Industrial Grants (including pharmaceutical)

PG Private Foundation Grants (including internal Penn grants) O Other

** For program projects, specify whether PI, co-leader or project leader. For center, core and training grants, similarly specify your role. *** Include any additional, brief information. For clinical trials, for example, specify if multicenter or single center and indicate role of Penn site. Explain any grants in “Other” category.

If space is needed for more entries, use an additional sheet.

14

CURRENT

Name of Grant & sponsor number

Funding Agency

Period of Grant

Type of Grant

Role in Grant and percent effort

Annual Direct Cost

Annual Indirect

Cost

Additional Comments

Peripheral immune regulation in autoimmune and neoplastic diseases

Kennel Club 05.01.17 to

04.30.21

PG Principal Investigator

5%

52,537 N/A This was a personal award to

support my research, following

an international, competitive application

process

The impact of intravenous anesthetic agents on canine natural killer cell cytotoxic function: the Achilles’ heel in cancer diagnosis and surgery?

American Kennel Club Acorn Award

01.05.18 to

02.05.19

PG Principal Investigator

3%

13,838 1,107 Pilot study to generate preliminary

data to support a larger application

Antigen-specific immunotherapy of myasthenia gravis in domestic dogs – a pilot study

Institute for Translational Medicine and Therapeutics (Program in Comparative

Animal Biology)

02.01.18 to

01.01.20

O Principal Investigator

5%

45,000 total

N/A Pilot study to generate preliminary

data to support a larger application

Canine retinal disease models for translational photoreceptor regeneration

National Eye Institutes, National

Institutes of Health

09.01.18 to 08.31.23

PP Co-Investigator

5%

574,016 350,150

15

PENDING

Name of Grant & sponsor number

Funding Agency

Period of Grant

Type of Grant

Role in Grant and percent effort

Annual Direct Cost

Annual Indirect

Cost

Additional Comments

A Comparative Biomedical Scientist Training Program at Penn Vet

National Cancer Institute, National

Institutes of Health

10.19 to 10.24

TG Co-Principal

Investigator 5%

N/A N/A This training grant will pay the tuition fees and stipend for two years of a clinical

residency at Penn Vet and up to four years of a PhD laboratory research project at

the NIH, for all successful

candidates. It is a vehicle for starting a

residency/PhD program at Penn Vet.

16

ACADEMIC COMMITTEES AT ROYAL VETERINARY COLLEGE:

DEPARTMENT COMMITTEES:

Chairman of Departmental Safety Committee, Department of Immunology, Imperial College London, UK (2004-2008)

Undergraduate Research Opportunities Program Coordinator, Department of Immunology, Imperial College London (2004-2008)

SCHOOL COMMITTEES:

Member of Genetically Modified Organisms Committee, Royal Veterinary College (RVC), UK (2008 to 2013)

Chairman of Phase 3 Sub-Committee overseeing Intramural Clinical Rotations, Didactic Clinical Elective modules and Research Project 2, before its disbanding; RVC (2009 to 2011)

Member of Learning, Teaching & Assessment and Course Management Committees, RVC (2009 to 2014)

Director of Research Project 2 (Final Year Student Research Project), RVC (2009 to 12/2016)

Director of Elective Teaching Program, RVC (2009 to 2015)

Member of Quality Assurance and Enhancement Strategy Working Group, RVC (2011 to 2014)

Academic Lead of the Oncology Special Interest Group (team of ~60 academics, including postdoctoral fellows and PhD students, with an interest in oncology), RVC (2012 to 12/2016)

ACADEMIC COMMITTEES OUTSIDE OF ROYAL VETERINARY COLLEGE:

THESIS COMMITTEES

Have served as a PhD examiner: o Department of Veterinary Clinical Sciences, University of Bristol, UK: Dr Iain Peters, 2005 o Centre for Rheumatology, University College London, UK: Dr Jenny McGovern, 2012 o Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Australia: Ms

Nathalee Prakesh, 2016 (pending)

Have co-supervised 2 PhD students registered at Imperial College London, UK, both of whom successfully graduated within four years:

o Dr Eduardo Fernandes MD (PhD: Imperial College London 2010) ‘Development and application of allograft-specific T regulatory cells'

o Dr Amira Fayad MD (PhD: Imperial College London 2010) ‘Transplantation tolerance through modulation of dendritic cells’

Served as PhD advisor to Dr Dana Kidder MD (PhD: University of Dundee, UK: 2011) ‘The role of sialoadhesin in the pathogenesis of systemic lupus erythematosus’

Currently serving on the PhD thesis committee for Dr Agostino Buono, registered at the Texas A&M College of Veterinary Medicine & Biomedical Sciences in the laboratory of Dr Joerg Steiner

‘Novel biomarkers for intestinal inflammation in canine chronic enteropathy’

STUDENT TRAINING:

Predoctoral:

STUDENT PROJECT SUPERVISION

ROYAL VETERINARY COLLEGE

BVetMed (Research Project 2: 8 to 14 weeks, Final Year)

Have supervised 30 RP2 projects (11 laboratory-based), performed as part of the requirements for fulfillment of the BVetMed degree, 13 of which achieved Distinctions: o 2005: Madeleine Lindsay (Distinction) o 2008: Charlotte Palmer (Distinction), Kate Williams (Distinction), Alexia Wilson (Merit), Alden

Chadbourne (Distinction and prize medal winner), Aysha Kent (Distinction) o 2009: Kate Walker (Distinction and prize medal winner), Andre Takano-Knuchel (Distinction),

Carolyn White (Distinction) o 2011: Clare Downes (Distinction), Emily Stoker (Merit), Tsai Payn (Pass)

17

o 2012: Jennifer Lawrence (Distinction), Hannah Bryant (Distinction), Charlotte Harvey (Pass) o 2013: Bosco Leung (Distinction), Erin Hadox (Merit), Sophie Lavender (Distinction) o 2014: Alexandra Weinberg (Pass), Amy Hughes (Merit), Kelly Ludden (Merit), Rebecca Swift

(Merit) o 2015: Eloise Dartnell (Merit), Harriet Bradford (Pass), Lily Raeyen (Merit), Michael Bignall

(Merit), Rebekah Carling (Merit) o 2016: Clare Parker (Merit), Connor Hawes (Merit), Lawrence Crossfield (Distinction)

BSc (Veterinary Pathology)

2008: Richard Appleton (Grade: 2(i))

2014: Eleanor Snee (Grade: 2(ii))

2015: Luca Fortuna (Grade: 1, First)

PhD students

Principal PhD supervisor for seven PhD students, all of whom have successfully graduated (all within four years): o Ms Clare Monk (MSc/PhD: University of London 2007)

‘Functional and molecular characterisation of regulatory T cells in the MRL/Mp mouse, a polygenic model of human lupus’

o Ms Flavia Rovis (PhD: University of London 2008) ‘Molecular mechanisms of peripheral tolerance: the murine CD4+CD25+ regulatory T cell’

o Mr Yogesh Singh (PhD: University of London 2009) ‘CD4+CD25+Foxp3+ regulatory T cell function in the NOD murine model of type I diabetes

mellitus’ o Ms Dammy Pinheiro (PhD BBSRC CASE Award: University of London 2010)

‘Phenotypic and functional characterisation of a CD4+CD25high FoxP3high regulatory T cell population in the dog’

o Ms Kate Carney (PhD BBSRC CASE Award: University of London 2014) ‘Amphiregulin – a novel regulatory T cell effector molecule?’

o Ms Elizabeth Slack (Wellcome Trust Integrated Veterinary Fellowship: RVC 2013) ‘The role of phosphatidylinositol 3-kinases in regulatory T cell function’

o Ms Julia Wu (RVC intra-mural funds and CRUK via QMUL, 2018) ‘Phenotypic and functional characterisation of CD4highFoxP3high regulatory T cells in canine

diffuse large B cell lymphoma’

IMPERIAL COLLEGE LONDON

MSc students

Have supervised 13 six-month MSc projects, nine of which achieved Distinctions; five of these students have subsequently successfully completed PhDs and four are currently undertaking PhDs: o 2002: Maria Spachidou: Tregs in murine lupus (Distinction) o 2003: Bob Lee: Proteomic characterization of murine Tregs (Pass) o 2003: Clare Monk: Tregs in murine lupus (Distinction) o 2005: Abubakar Omar: Heat shock proteins and Tregs (Pass) o 2006: Flavio Sacchini: Characterization of canine Tregs (Distinction) o 2007: Kalyani Panse: Tregs and dendritic cells in murine lupus (Distinction) o 2009: Ksenija Jovanovic: Glycobiological studies of murine Tregs (Distinction and Richard

Bachelor Prize) o 2010: Charlotte Grant: Characterization of canine CD4+ Tregs (Distinction) o 2011: Win-Yan Chan: Expression of epidermal growth factor ligands by murine regulatory and

conventional T cells (Merit) o 2012: Tim D’Alessandri: Investigating the pathogenic role of regulatory T cells in canine

lymphoma – a model of human non-Hodgkin’s lymphoma (Distinction) o 2013: Kiruthika Manivannan: Regulatory T cells in DAF and C3 knock-out mice: peripheral

tolerance gone bad? (Distinction) o 2014: Julia Wu: Towards a flow cytometric assay for canine IL-10: a proof-of-principle study

(Distinction) o 2015: Konstantinos Efstathiou MD: Towards the characterization of canine tolerogenic dendritic

cells: a preliminary study (Merit)

18

Postdoctoral:

ROYAL VETERINARY COLLEGE

Clinical Training Scholars (Interns)

Have contributed to the training of 45 Clinical Training Scholars while they were rotating though the Internal Medicine Service, including: o 2005: Ian Jones, Stephanie Keele, Yaiza Forcada o 2006: Matthew McMillan, Alice Courtney, Gareth Buckley o 2007: Beth Walding, Suzanne Bailey o 2008: Tara Bellis, Francesca Soutter, Nicolas Woodbridge, Matthew Atherton o 2009: Catherine Davidson, Jonathan Hardy, Carl Bonello-Cole o 2010: Stephanie Lalor, Laura Cosgrove, Georgina Harrison, Kim Hsu, Giacomo Stanzani o 2011: Annabel Beattie, Thomas Cardy, Charlotte Dawson, Sophie Keyte, Efa Llewellyn o 2012: Daniel Preece, Jesabelle Kibanda, Andrew Gibson, James Swann o 2013: Chloe Burney, Laura Heaps, Lee Beever o 2014: Heather Covey, Victor Ortiz, Caitlin Tzounos, Rebecca Geddes o 2015: Natasha Williams, Poppy Gant, Albane Fauron o 2016: Kristina Lehnus, Louise Corah, Kenneth Sherwood, Georgina Harris, Felicity Moffatt,

Katherine Smallwood

Senior Clinical Training Scholars (SCTSs; Residents)

Have contributed to the training of 56 Senior Clinical Training Scholars (SCTSs): o Internal Medicine SCTSs (29): Richard Elders, Lucy McMahon, David Walker, Stijn Niessen,

Nicole Bridger, Barbara Glanemann, Kirsty Roe, Isuru Gajanayake, Silke Schmitz, Kerstin Gesierich, Rosanne Jepson, Lucie Goodwin, Alex Rose, Sina Marsillo, Ruth Gostelow, Fabio Procoli, Kristine Jensen, Kristin Anfinsen, Michaela Schnyder, Christopher Scudder, Sophie Keyte, Anna Threlfall, Sarah Steinbach, James Swann, Karolina Scahill, Heather Covey, Joana Aguiar, Jessica Florey, Vanessa Woolhead, Rebecca Geddes, Hannah Darcy, Myles McKenna, Rungrote Osathanon

o Cardiology SCTCs (9): Babis Koffas, Simon Dennis, Pierre Menault, Tobias Wagner, Imke Maerz, Paul Motskula, Kieran Borgeat, Julia Sargent, Ilaria Spalla

o Emergency and Critical Care SCTCs (5): Chiara Valtolina, Shailen Jasani, Karen Humm, Caroline Hirst, Alexander Hatch

o Oncology SCTSs (4): Francesca Fabrizio, Angela Taylor, Sofia Carvalho, Shasta Lynch o Surgery SCTCs (2): Mickey Tivers, Kevin Parsons o Neurology and Neurosurgery SCTCs (2): Gemma Walmesley, Joe Fenn o PhD students on clinics (1): Aarti Kathrani

SCHOOL OF VETERINARY MEDICINE, UNIVERSITY OF PENNSYLVANIA

Interns

Have contributed to the training of 7 Interns while they were rotating though the Internal Medicine Service, including:

o 2017: Alexandra Crooks, Megan Murray

o 2018: Marie Keith, Sarah McCalla, Christian Munevar, Caroline Murray

o 2019: Sarah Winter

Residents

Have contributed to the training of 14 Residents:

o Internal Medicine (14): Jodie Anderson, Stephen Cai, Matthew Lechner, Olivia Nathanson, Cara

Steele, Jennifer Forgash, Brittany Fowler, Bridget Harvey, Mei Lun Mui, Emily Seidel, Emily

Shea, Rebecca Stevens, Alison Thomas-Hollands, Sindumani Manoharan

SEMINARS AND ORAL PRESENTATIONS BY INVITATION: (Poster presentations not included) Scientific

Session Chairman – ‘Glycoimmunology’ at the British Society for Immunology Congress, Harrogate, December 8, 2005: 8 pages

Session Chairman – ‘Regulatory T Cells: Ontogeny to Fundamental Mechanisms’ at the British Society for Immunology Congress, Glasgow, February 22, 2007: 10 pages

Speaker – ‘Identifying T Cell Subset Phenotype and Function’; one-day meeting, Welwyn Garden City,EuroSciCon, March 16, 2007

19

o ‘Functional characterization of CD4+CD25+ regulatory T cells – implications for the pathogenesis of autoimmune disease’

o Speaker – ‘Lymphocyte Regulation’ as part of the annual three-day Immunology Short Course, Imperial College London, Hammersmith Campus, November 2010 to present

Conference Chairman – ‘Regulatory T Cell Biology’ conference, Institute of Child Health, University College London, EuroSciCon, March 5, 2010: 20 pages

Conference Chairman – ‘Regulatory T Cells and T Helper Cells: Fundamental Biology and Translational Research’, RVC Camden Campus, March 30, 2012: attracted six world-class speakers (five from UK; one from Germany), over 70 delegates and 11 exhibitors: 30 pages

Conference Chairman – ‘Regulatory T Cells and T Helper Cells: Fundamental Biology and Translational Research’, RVC Camden Campus, April 25, 2014: attracted six world-class speakers (five from UK; one from Germany), over 80 delegates and 16 exhibitors: 35 pages

Conference Chairman – ‘The Tumour Microenvironment and Regulatory Cells: Fundamental Biology and Translational Research’, RVC Camden Campus, April 17, 2015: attracted 10 world-class speakers from the UK, over 90 delegates and 12 exhibitors: 35 pages

Invited presentations on regulatory T cell research (academia and industry): >35 since 2003, including presentations given at the School of Veterinary Science, University of Bristol; Invitrogen-Dynal (Oslo, Norway); Sanofi-Pasteur (London Business School); UCB Celltech (Granta Park, Cambridge); Pfizer (Sandwich, Kent); Veterinary Laboratories Agency (Weybridge); Babraham Institute (Babraham, Cambridge); GlaxoSmithKline (Stevenage); and Essen Bioscience, Merial Animal Health, Zoetis, Poiesis Ltd, Genesis (RVC, Hawkshead and Camden Campuses).

Clinical

Short Course Organizer (BSAVA):o ‘Immune-mediated disease in dogs and cats: state of the art’ (September 2003 with Professor

Michael Day): 55 pages o ‘Immune-mediated disease in dogs and cats: the theory and the practice’ (November 2008 with Dr

Nathaniel Whitley): 20 pages

Short Course Organizer (RVC CPD Unit):o ‘Diagnosis and management of diseases of the immune system in the dog and cat’ (November

2010, November 2012, November 2014 and November 2016, all with Dr Nathaniel Whitley): Each 100-105 pages

Speaker (RVC CPD Unit): ‘Rational use of antimicrobials in veterinary practice’ (November 18, 2015)

‘Antimicrobial treatment of respiratory disease in dogs and cats’: 12 pages

Speaker, Medical Highlights from the QMHA (RVC CPD Unit):o February 2010:

‘A case of bacterial endocarditis in a miniature poodle’ (steeple-chase case) o February 2011:

‘Update on immune-mediated diseases’

‘Treatment of immune-mediated diseases – beyond prednisolone’

Speaker, Northern Veterinary Show (Nurses’ Stream):o ‘Autoimmune disease in dogs and cats: when the immune system goes bad’ (June 25, 2011)

Part 1: Clinical overview: 4 pages

Part 2: Treatment options: 10 pages

Speaker, London Veterinary Show (Veterinarians’ Stream):o ‘The diagnosis of IMHA and IMTP: maximizing the value of the pathology pound’ (November 22,

2013): 4 pages

Conference presentations and other invited international lectures:o ‘Genetic modelling of intestinal disease: the paradigm of gluten-sensitive enteropathy in the Irish

setter dog’ Ninth Annual Congress of European College of Veterinary Internal Medicine (ECVIM), October 14-16, 1999, Perugia, Italy: 3 pages

o ‘Polysystemic immune-mediated disease in the dog and cat: a review for the practitioner’ Ninth Annual Congress of ECVIM, October 14-16, 1999, Perugia, Italy: 4 pages

o ‘Gastrointestinal immunity in health and disease: an overview’ Tenth Annual Congress of ECVIM, September 14-16, 2000, Neuchatel, Switzerland (State-of-the-Art): 4 pages

o ‘Mechanisms of peripheral immune tolerance: all creatures great and small’ Twentieth Annual American College of Veterinary Internal Medicine (ACVIM) Forum, May 29 – June 1, 2002, Dallas, Texas (Specialist stream): 3 pages

o ‘Transplantation tolerance in the 21st Century: the art of the impossible?’ Twenty-First Annual ACVIM Forum, June 4-7, 2003, Charlotte, North Carolina (Specialist stream): 3 pages

o ‘Immunodeficiency diseases of the dog and cat: a general overview’ Twenty-Second Annual ACVIM Forum, June 9-12, 2004, Minneapolis, Minnesota (Generalist stream): 3 pages

20

o ‘The gastrointestinal-associated lymphoid tissue: graveyard or oasis of the mucosal immune system?’ Twenty-Fifth Annual ACVIM Forum, June 6-9, 2007, Seattle, Washington (Specialist stream) (ECVIM Guest Lecture #1): 3 pages

o ‘The immunological battleground of the airways: who’s talking to whom?’ Twenty-Fifth Annual ACVIM Forum, June 6-9, 2007, Seattle, Washington (Specialist stream) (ECVIM Guest Lecture #2): 3 pages

o ‘An immunology update for internists: making sense of cells and molecules of the innate and adaptive immune system’ Twenty-Sixth Annual ACVIM Forum, June 4-7, 2008, San Antonio, Texas (Specialist Stream): 3 pages

o ‘Characterization of canine CD4+ regulatory T cells’ Twenty-Seventh Annual ACVIM Forum, June 3-6, 2009, Montreal, Canada (Specialist Stream): 3 pages

o ‘Characterization of CD4+CD25highFOXP3high regulatory T cells in the dog’ Seoul National University (SNU) School of Veterinary Medicine, July 14, 2010, Seoul, ‘Brain Korea 21’ Program- sponsored presentation to SNU faculty

o ‘Immune-mediated disease – a clinical overview’ Konkuk University Faculty of Veterinary Medicine, July 14, 2010, Seoul, Korea, Neodin Veterinary Science Institute-sponsored presentation to general practitioners

o ‘Immunosuppressive therapy – the theory and the practice’ Konkuk University Faculty of Veterinary Medicine, July 14, 2010, Seoul, Neodin Veterinary Science Institute-sponsored presentation to general practitioners

o ‘Of mice, men and dogs? A population of canine CD4+CD25highFOXP3+ T cells exhibits suppressive properties in vitro’ Twentieth Annual Congress of ECVIM, September 9-11, 2010, Toulouse, France: 2 pages

o ‘All creatures great and small: a review of regulatory T cells in mice, humans, dogs and other domestic species’ Thirtieth Annual ACVIM Forum, May 30 ‒ June 2, 2012, New Orleans, Louisiana (Specialist Stream): 3 pages

o ‘Canine multicentric B cell lymphoma – the importance of Tregs’ Thirty-First Annual ACVIM Forum, June 12-15, 2013, Seattle, Washington (Specialist Stream)

o ‘Flow cytometry for the internist – state of the art’ Thirty-Third Annual ACVIM Forum, June 2-6, 2015, Indianapolis, Indiana (Specialist Stream): 3 pages

o 1. ‘Companion animals in comparative oncology: One Medicine in action’; 2. ‘Veterinary Clinical Immunology: a new sub-specialty of internal medicine?’; 3. ‘IMHA 101: an update on pathophysiology and diagnosis’. All at the Thirty-Fourth Annual ACVIM Forum, June 8-11, 2016, Denver, Colorado: 4 pages each Explanatory note: The session entitled ‘Veterinary Clinical Immunology’ represented a four-

hour interactive session of the nascent Veterinary Clinical and Comparative Immunology Society I have developed from a Clinical Immunology Special Interest Group hosted on the ACVIM website. Established by me, this group includes specialists in internal medicine, emergency and critical care, infectious disease, oncology, dermatology, gastroenterology, clinical and anatomical pathology, and neurology, representing a global community of almost 250 members whose aim is to advance the science and practice of small animal clinical immunology. Our first task was to undertake scoping reviews of key themes within each of our task forces, which were reported in this session. This represents a work in progress, but is an exciting initiative that has gained considerable traction in a short space of time.

o 1. ‘Small Animal Internal Medicine Consensus Statement: Diagnosis of Immune-Mediated Hemolytic Anemia’; 2. ‘Small Animal Consensus Statement: Treatment of Immune-Mediated Hemolytic Anemia’. Both at the Thirty-Sixth Annual ACVIM Forum, June 14-16, 2018, Seattle, Washington: 3 pages each

o ‘Diagnostic Criteria for IMHA: Laying the Ground Rules, or Opening Pandora’s Box?’ American College of Veterinary Pathology / American Society of Veterinary Clinical Pathology Concurrent Annual Meeting, November 3-7, 2018, Washington, District of Columbia: 3 pages

o 1. ‘Evidence for Non-Infectious Triggers of Immune-Mediated Hemolytic Anemia’; 2. ‘Evidence for Infectious Triggers of Immune-Mediated Hemolytic Anemia’. Both with Dr. Linda Kidd, Western University of Health Sciences. Both at the Thirty-Seventh ACVIM Forum, June 5-7, 2019, Phoenix, Arizona: 3 pages each

o ‘Assessment of Immune Cell Functions’ Twenty-Ninth Annual Congress of ECVIM (Clinical Pathology Stream), September 19-21, 2019, Milan, Italy: 3 pages

21

TEACHING RESPONSIBILITIES:

IMPERIAL COLLEGE LONDON

2001-2009

o Intercalated BSc (Foundations of Science): Module 1: Immune Activation and Memory

(extended tutorials: 4 hours); Module 3: Immunological Tolerance and Transplantation (lecture: 1 hour); Module 4: Autoimmunity, Immunotherapy and Tumor Immunology (lecture: 1 hour; supervision of oral presentations: 3 hours)

o MSc (Immunology): Module 1: Recognition of Antigen (supervision of oral presentations: 3 hours); Module 2: Regulation of Immune Responses (lecture 1 hour); Module 3:

Transplantation (lecture: 1 hour; practical sessions: 4 hours); Module 4: Allergy, Inflammation and Autoimmunity (lecture: 1 hour); all modules (15 weekly 2-hour tutorial

sessions)

o PhD (Wellcome 4-Year PhD Program): Essentials of Immunology (First Year course; extended tutorial: 3 hours)

2010-present

o MSc (Immunology): Module 2: Regulation of Immune Responses (lectures: 2 hours); Module 3: Transplantation and Autoimmunity (lecture: 1 hour)

2013-present:

o MSc (Immunology): As above plus additional one-hour lecture in Module 2

2009-present

o Immunology Short Course for Clinicians and Scientists: Day 1: Fundamentals of Immunology (lecture: 1 hour)

ROYAL VETERINARY COLLEGE

2004-2011

o BVetMed Elective Program: Showcase presentations for whole program (lectures 6 hours) o North American Veterinary Licensing Examination (NAVLE®) revision session (small animal

internal medicine; lecture: 1 hour)

2004-12/2016

o BVetMed Professional Strand: ‘Immune-mediated diseases: diagnosis and treatment’ (lectures: 2 hours)

o BVetMed Haemolymphatic Module: ‘Feline infectious diseases’ (lectures: 2 hours)

o BVetMed Haemolymphatic Module: ‘Bleeding disorders’ (directed learning: 6 hours)

o Introduction to Research Project 2 (lecture: 1 hour) o BVetMed Elective Program: Various – lectures (5 hours), directed learning (3 hours),

practical (3 hours)

2011-12/2016

o BSc (Veterinary Pathology): Immunopathology Module (lectures: 2 hours)

22

TEACHING CHRONICLE – page 1 (since time of last promotion: September 2013; all at RVC unless otherwise indicated)

FACULTY NAME

Academic Year

Semester Course # Course Name Student Number

Topic Lecture Hours

Lab Hours

2013 Spring N/A MSc (Immunology) Module 3: Transplantation and Autoimmunity

~35 Systemic lupus erythematosus 1

Summer N/A BVetMed Professional Strand 240 Immune-mediated diseases: diagnosis and treatment

2

N/A BVetMed Haemolymphatic Module

240 Feline infectious diseases 2

N/A BVetMed Haemolymphatic Module

240 Bleeding disorders 6

N/A BVetMed Elective Program ~90 Feline Medicine Elective 2 6 N/A BVetMed Elective Program ~90 Internal Medicine Elective 3

Fall N/A BVetMed Professional Strand 240 Introduction to Research Project 2 1

N/A BSc (Veterinary Pathology) Immunopathology Module

~25 T cell regulation 2

N/A MSc (Immunology) Module 2: Regulation of Immune Responses

~35 T cell regulation Regulatory T cell biology

3

N/A Immunology Short Course for Clinicians and Scientists

~40 Regulation of the immune system 1

Total lecture hours = 17; total lab hours = 12

2014, 2015, 2016

ALL AS ABOVE

2017 Spring VPTH604 VMD First Year (at Penn Vet) 130 Immunodeficiency diseases 2 0 2018 Spring VPTH604 VMD First Year (at Penn Vet) 130 Immunodeficiency diseases 2 0 2018 Fall VMED600 VMD First Year (at Penn Vet) 10 Physical Examination, Cat and Dog 0 2 2019 Spring VPTH604 VMD First Year (at Penn Vet) 130 Immunodeficiency diseases 2 0 2019 Spring Responsible

Conduct of Research

Biomedical Graduate Studies Third Year PhD students

13 Collaborative Science, Use of Animals in Research, Use of Humans in Research

0 2