upic internship 2015
TRANSCRIPT
Baruch Summer Interns 2015 Rebecca Glover
Biosystems Engineering
Bioprocessing Emphasis
Minor: Sustainability
Alston Loper Biosystems Engineering Ecological Emphasis Minor: Sustainability
Ashleigh HoughBiosystems Engineering Ecological Emphasis Minor: Environmental
Engineering
Raymond MiddleswarthBiosystems Engineering
Bioprocessing Emphasis
Samplingwe collected grab samples monthly along
flow paths in eight different locations between DeBordieu and Strawberry
DeBordieu (Pond-Wetland)
Strawberry (“Natural”)
4
1
23
Parameters Sampled in the Field
• pH• dissolved oxygen • water level• water temperature• specific
conductance• turbidity • oxygen reduction
potential
Lab Work
• water sampling procedure/handling
• filtration of TSS/VSS (suspended solids)
• bacteria processing/analysis• filtration for nutrient analysis • standard curve/reagent for
nutrient analysis– creating a work list/file
designation • digestions for TKN and TKP• organic carbon analysis
Changes in E-coli bacteria levels (MPN) in Strawberry and Debordieu (6/1/14 -10/1/14)
Membrane Filter Technique
• Used for enumerating coliforms and fecal coliforms in drinking water
• Precise and easy to replicate
Membrane Filter Technique
After 22 hr incubation at 350C
Dark colonies with metallic sheen are verified total coliforms
Fecal coliforms: • tested simultaneously• Dark blue colonies are
positive
Membrane Filter Technique
Verification:• Lauryl Tryptose-Total coliform • Test positive for total coliforms
if gas produced within 48hr
Confirmed Phase• Brilliant Green Bile Broth 2%• Positive for fecal coliforms;gas
production within 48hr
Gas production within 18hr goes to confirmed phase
Digital Elevation Models (DEMs)
Entire Black Creek Watershed= 2,100 acres
Northwoods Watershed(includes elementary school)= 58 acres
Belhaven Watershed (includes Harvest Church site) = 257 acres
Northwoods Elementary School Watershed
0.1 0.25 0.5 1-0.10.00.10.20.30.40.50.6
Simulated Rainfall (in.)
Pred
icte
d Ru
noff
Volu
me
(in.)
0.1 0.25 0.5 1-0.10.00.10.20.30.40.50.6
Simulated Rainfall (in.)
Pred
icte
d Ru
noff
Volu
me
(in.)
0.1 0.25 0.5 10.0
0.1
0.2
0.3
0.4
0.5
0.6
Simulated Rainfall (in.)
Pred
icte
d Ru
noff
Volu
me
(in.)
0.1 0.25 0.5 10.0
0.1
0.2
0.3
0.4
0.5
0.6
Simulated Rainfall (in.)
Pred
icte
d Ru
noff
Volu
me
(in.)
5% Impervious Area
25% Impervious Area 50% Impervious Area
10% Impervious Area
Northwoods Elementary School Watershed (58 acres)
