using fijiwings2.1 to study cell growth shipman and dobens, gsa prep
DESCRIPTION
Cell cycle https://en.wikipedia.org/wiki/Cell_cycle#/media/File:Cell_Cycle_2-2.svgTRANSCRIPT
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Using Fijiwings2.1 to study cell growth
Shipman and Dobens, GSA PREP
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Tissue GrowthIncrease in cell number, size or extracellular matrix (often swells to absorb water)
proliferation growth matrix production
Dobens, unpublished
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Cell cycle
https://en.wikipedia.org/wiki/Cell_cycle#/media/File:Cell_Cycle_2-2.svg
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Regulation of the cell cycle
https://en.wikipedia.org/wiki/Cell_cycle#/media/File:Signal_transduction_pathways.svg
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Intrinsic and extrinsic signals control growthFlies get big as larva, depending on whether well-fedCell measures ATP content via TOR signaling pathwayOrganism uses widespread growth factors and hormones to regulate cell size and cell proliferation: Insulin-like growth factor
https://en.wikipedia.org/wiki/File:2nd_bio_diagram_2.jpg
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Insulin signaling required for animal growth
Reduction of insulin signaling in fly Reduction of insulin signaling in mouse
WT fly Trbl-expressingfly
Fat bodycells
Normal mouse
Dwarf mouse
Dobens, unpublished
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Identifying intervein regions
Dobens, unpublished
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Trbl misexpression in the wing
Dobens, unpublished
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Fijiwings2.1: desktop
• “Action Bar”• Fiji tools are still available
Dobens, unpublished
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Using Fiji plug-ins to detect trichomes
• ‘detect local maxima’
• Micrograph must be in focus, high contrast
Dobens, unpublished
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Trichome identification
• While precision 100%, accuracy 96%
Dobens, unpublished
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GAL4 misexpression system
X
Dobens, unpublished
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Fijiwings: ‘polygon trichome density’ tool
Dobens, unpublished
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Segmentation
Dobens, unpublished
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Intervein area and trichome density
Dobens, unpublished
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Heat map of tissue size
Dobens, unpublished
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Identification of trichome density
Dobens, unpublished
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Size heat maps and trichome density cool maps
• Available for download at sourceforge.net
Dobens, unpublished
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Trbl RNAi enhances PtenRNAi
Dobens, unpublished