Vectores de Expressão, Etiquetas epitópicas

http://www.medigenomics.de/en/molbio1techinfo2E.html Czar et al. (2008)

http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606

http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606

http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=155864

http://www.bio.psu.edu/People/Faculty/Akashi/weak_selec.htm

  Permite modificações da sequência original de codões pouco expressos por codões mais utilizados no organismo receptor que codifiquem o mesmo aminoácido.

Palmiter et al. (1991) Kim et al. (1995)

Harraghy et al. (2008)

http://en.wikipedia.org/wiki/Kozak_consensus_sequence

http://www.promega.com/paguide/chap11.htm

HIS6

N-HHHHHH-polipéptido-de-interesse-C

http://www.emdbiosciences.com/html/NVG/Ecoli_Vectors.html

Lane Sample 1 Perfect Protein ™ Markers 10–225 kDa 2 Total cell protein (TCP) 3 Soluble fraction 4 Total cell protein (TCP) 5 Soluble fraction 6 Ni2+ His•Bind® Fractogel eluate pool 7 Strep•Tactin® eluate pool 8 Thrombin digest 9 Post-thrombin His•Bind flowthrough

Harrison (2008)

NusA Proteína X

Chaperones (GroEL / GroES)

Desnaturação / Agregação

(inibe)