vinnitsa national pirogov memorial medical university / department of microbiology
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Pathogenic vibrios. Vibrio cholerae. Laboratory diagnostics, treatment and prophylaxis of cholera. Vinnitsa National Pirogov Memorial Medical University / Department of microbiology. TAXONOMIC POSITION OF THE PATHOGENIC VIBRIOS. Family Vibrionaceae Genus Vibrio Medical important species: - PowerPoint PPT PresentationTRANSCRIPT
Pathogenic vibrios. Vibrio cholerae. Laboratory diagnostics, treatment
and prophylaxis of cholera.
Vinnitsa National Pirogov Memorial Medical University / Department of microbiology
TAXONOMIC POSITION OF THE PATHOGENIC VIBRIOS
• Family Vibrionaceae
• Genus Vibrio
• Medical important species:
• V.cholerae
• V.parahaemolyticus
• V.vulnificus
• V.alginolyticus
Classification of the vibrios
• Biochemical classification by Heiberg (1934)
• Serological classification
VIBRIO CHOLERAE
Cultural characteristics• The cholera vibrions is strongly aerobic • Growth is better in an alkaline medium the
range of pH being 6.4-9.6 (optimum 8.2)• Special media:• Alkaline peptone water at pH 8.6;• Monsur's taurocholate tellurite peptone
water at pH 9.2.• Alkaline bile salt agar (BSA) pH 8.2.
Classification of the V. cholerae
Biovars:
•V. cholerae b/v classical
•V. cholerae b/v El-Tor
Serotypes:
•Ogawa (AB)
•Inaba (AC)
•Hikojima (ABC)
Distinguishing features of biovarsTests b/v
classicalb/v El Tor
Agglutination of fowl erythrocytes - +
Lysis of sheep erythrocytes - +
Growth in the presence of polymixin B
- +
Sensitivity to phages Mukerjee`s group IV phage (C)
Mukerjee`s group V phage (El -Tor)
Acetoin production in the Voges-Proskauer test
- +
Factors of virulence• Exotoxin (choleragen, cholera enterotoxin,
cholera toxin, CT, or CTX)• Endotoxin.
• Adherence factors (pili)
• Proteolytic enzymes (gelatinase, mucinase)
Epidemiology and pathogenesis
• Infection is acquired through fecally contaminated water or food.
• Clinical forms:
1.Enteritis
2.Gastroenteritis
3.Choleric algid
4.Choleric coma (severe dehydration)
Laboratory diagnosis
• Rapid tests
• Microscopy
• Bacteriological method
• Serology
Prophylaxis
• General measures
• Specific measures
Vibrio cholerae