wake-up 1.what do restriction enzymes do? 1.restriction enzymes are naturally produced by bacteria....
TRANSCRIPT
Wake-up
1. What do restriction enzymes do?
2. Restriction enzymes are naturally produced by bacteria. Explain why they have them.
2. Explain the difference between a blunt cut and a staggered cut.
Homework
Check the answers on your homework. Find the link on the Wiki, “Genetic Engineering SG
KEY”.
Applications of Genetic Engineering
Transgenic OrganismAn organism (animals, plants, and bacteria)
that has been genetically altered.
Example #1: Diabetes and Insulin (Medical)
What is Insulin?Insulin (protein)
helps glucose move from our bloodstream to
our cells
Glucose is from food that was
consumed
Type 1 Diabetes: The Pancreas does not produce insulin
Diabetics need to take Insulin shots
How does someone with Type 1 Diabetes get insulin?
History of Insulin Production
1974 – Pancreas was removed from slaughtered animals and purified for
human use
History of Insulin Production (finish)
1980’s – Gene that makes insulin in humans was inserted into E.coli (Bacteria) which produced synthetic human insulin
Example #3: Genetically Modified Food (GMO’s)
What are Genetically Modified Organisms (GMO’s)?
An organism whose genetic material has been altered using genetic engineering
techniques
Why are Organisms Genetically Modified?
Modified to be resistant to: •Pests•Disease•Cold
•Drought•Salt
Also an overall “better” quality
Example #1 of GMO: Golden Rice
Malnutrition is common in 3rd world countries; impoverished peoples rely on rice
for the main staple of their diet.
However, rice does not contain adequate amounts of all necessary nutrients to prevent
malnutrition
More than 10 million children die of malnutrition every year. A vast majority of these deaths is linked
to micronutrient deficiencies.
Vitamin A deficiencies can damage the
immune system and decrease
the body’s ability to resist
or fight infections or can lead to
partial to totalblindness
Golden rice was genetically engineered to contain beta carotene, which can provide Vitamin A
(Video: Beginning to 4:30) Watch me!!!
GMO Example #2:Tomato and Artic Flounder Video Clip
Watch me!!!
Example #3: Glowing Organisms (purpose unclear)
Which organisms naturally glow?
Can we make other organisms glow using Genetic Engineering?
When complete with notes, pick up the Paper Plasmid activity from the substitute. Also grab scissors and a few pieces of tape. (Leave tape
dispensers where they are, just take tape)
Work through the activity. Make sure to read the directions carefully.
When complete, answer the questions on the lab. Before you turn it in, staple the Recombinant DNA
with the insulin gene to your lab sheet.
Pick up your homework and work on it.