whitney humphrey dr. barbara taylor hhmi summer program
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![Page 1: Whitney Humphrey Dr. Barbara Taylor HHMI Summer Program](https://reader036.vdocuments.net/reader036/viewer/2022081514/56649d2e5503460f94a060e2/html5/thumbnails/1.jpg)
BRAIN AND BEHAVIOR: THE ROLE OF THE FRUITLESS GENE IN COMPLEX BEHAVIOR IN DROSOPHILA
Whitney HumphreyDr. Barbara TaylorHHMI Summer Program
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RELEVANCE OF WORK
Understanding of the interaction between the central nervous system and behavior.
Neurological diseases in humans Sex Determination and Sexual
Orientation in flies
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DROSOPHILA MELANOGASTER
Ideal model organisms for studying genetics Short generation time Many strains exists
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SEX-SPECIFIC BEHAVIORAL DIFFERENCES IN DROSOPHILA
Courtship Aggression
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MALE VS. FEMALE GENETICSIn females, a cascade is initiated and through splicing events produce a female-specific transcript encoding a female-specific protein:
-DoublesexF (DsxF)
In males, a cascade is initiated but different splicing events produce male-specific transcripts encoding male-specific proteins:
-FruitlessM (FruM)-DoublesexM (DsxM)
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IN FRU MUTANTS, MALE BEHAVIOR IS ALTERED
Expected behavior:-Males court females-Production of song-Copulation
Unexpected behavior:-Female-male courtship interrupted-Male-male courtship observed-This event is referred to as chaining.-May or may not be song but copulation does not occur
Deficiencies Fru3, Fru4
(P-element inserts)
Fru1
(Inversion)
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AVERAGE ACTIVITY LEVELS FOR CANTONS FLIES
DL Cycle: 12-hours lights on, 12-hours lights off
Males: Anticipate lights on and have very little activity during the day (siesta)
Females: Do not anticipate lights on and do not take a siesta.
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THE DROSOPHILA ACTIVITY MONITOR
How we measure activity:
The DL cycle:9:00 - 20:59 – Lights on21:00 - 8:59 – Lights off
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ACTIVITY OF WILDTYPE MALES
9:00
10:1
5
11:3
0
12:4
5
14:0
0
15:1
5
16:3
0
17:4
5
19:0
0
20:1
5
21:3
0
22:4
50:
001:
152:
303:
455:
006:
157:
308:
450
10
20
30
40
50
60 CSA Average Activity vs. Time
Time
Avera
ge C
ounts
Individual Raw Data
Lights on Lights off
n = 20
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DO FRU HYPOMORPHS MALES HAVE WILDTYPE ACTIVITY CYCLES?
9:00
11:0
0
13:0
0
15:0
0
17:0
0
19:0
0
21:0
0
23:0
01:
003:
005:
007:
009:
000
10
20
30
40
50
60fru1 Average Activ-
ity vs. Time
Time
Avera
ge A
cti
vit
y
9:00
11:3
0
14:0
0
16:3
0
19:0
0
21:3
00:
002:
305:
007:
300
102030405060
CSA Average Activity vs. Time
Time
Avera
ge C
ounts
9:00
11:0
0
13:0
0
15:0
0
17:0
0
19:0
0
21:0
0
23:0
01:
003:
005:
007:
009:
000
20
40
60
fru4 to1 Average Activity
Time
Acti
vit
y
9:00
10:4
5
12:3
0
14:1
5
16:0
0
17:4
5
19:3
0
21:1
5
23:0
00:
452:
304:
156:
007:
450
20
40
60
fru4tocs Average Activity vs Time
Time
Acti
vit
y
n = 20
n = 10n = 10
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FRUM NULL MUTANTS HAVE WILDTYPE ACTIVITY CYCLES
9:00
10:3
0
12:0
0
13:3
0
15:0
0
16:3
0
18:0
0
19:3
0
21:0
0
22:3
00:
001:
303:
004:
306:
007:
309:
000
10
20
30
40
50
60
fru440 x frusat15 Av-erage Activity
Time
Acti
vit
y9:
00
10:3
0
12:0
0
13:3
0
15:0
0
16:3
0
18:0
0
19:3
0
21:0
0
22:3
00:
001:
303:
004:
306:
007:
309:
000
10
20
30
40
50
60
fru440 x P14 Average Activity
Time
Acti
vit
y
n = 12n = 12
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CIRCADIAN RHYTHM
A roughly-24-hour cycle in the biochemical, physiological or behavioral processes.
CSA
Fru440 x Frusat15
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CONCLUSION
Initially, we investigated if mutations in fruitless affected the male pattern of activity in flies.
We conclude that fruitless does not affect sex-specific activity cycles. However, fruitless mutants show levels of
decreased activity. We also discovered that the fruitless
mutants with deficiencies did not have a 24-hour period.
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ACKNOWLEDGMENTS
Dr. Barbara Taylor Dr. Jaga Giebultowicz Will Gibson Eileen Chow Dr. Kevin Ahern HHMI Summer Undergraduate Research
Program