why cryogenic grinding?
DESCRIPTION
Why Cryogenic Grinding?. Many analytical samples are too flexible or sensitive to be ground at ambient temperatures Samples are subject to degrading Does not damage DNA or RNA. The Basics. - PowerPoint PPT PresentationTRANSCRIPT
© SPEX SamplePrep, Inc. 2011
Why Cryogenic Grinding?
• Many analytical samples are too flexible or sensitive to be ground at ambient temperatures
• Samples are subject to degrading• Does not damage DNA or RNA
© SPEX SamplePrep, Inc. 2011
The Basics
• Samples are chilled at Liquid Nitrogen (LN) temperatures and ground using a Magnetically-Driven Impactor– No mechanical linkages or bearings to fail
• Sealed Grinding Vials ensure sample integrity
• Touch screen display w/ stored procedures for rapid process replication and consistency
© SPEX SamplePrep, Inc. 2011
Who Can Benefit From a Freezer/Mill®?
• Forensic Labs – DNA identification• Medical Labs– genetic research, disease
prevention• Hospital Labs – For genetic screening• Agricultural Labs – For crop development• Agricultural Veterinary Labs – For livestock
breeding and health• Archealogy Labs – Ancient DNA extraction• Public Health and Environmental Monitoring Labs• QC Testing of Manufactured Goods• Electronics Companies – Grinding Electronic
Components
© SPEX SamplePrep, Inc. 2011
The Freezer/Mill® Family
Sample Size
0.6 – 5 g 0.6 – 100 g 0.6 – 200 g
LN System
Manual Manual, Auto-Fill Option Auto-Fill Only
Price $ $$ $$$
© SPEX SamplePrep, Inc. 2011
6970EFM – Features & Benefits
• Two Models Available– Single Grinding Chamber (6970M)
• 1 Grinding Chamber + 1 Pre-Cooling Chamber
– Dual Grinding Chambers (6970D)
• Totally enclosed auto-fill system with no Liquid Nitrogen exposure
• Increased through-put– Up to 8 small vials or 2 large vials
(D model only)
© SPEX SamplePrep, Inc. 2011
6770 – Features & Benefits
• Cost efficient• Runs one Micro-vial or small vial per run• Perfect for smaller samples (5 grams or less)• Additional area for pre-cooling samples • Unit has small counter footprint
© SPEX SamplePrep, Inc. 2011
6870 – Features & Benefits
• Versatile large grinding chamber– Runs a variety of vials, ranging from micro vials
through the large, even up to 4 of the small vials– Allows for wide range of sample sizes
• Available with Auto-Fill, or without
• Additional area for pre-cooling samples
© SPEX SamplePrep, Inc. 2011
Freezer/Mill Accessories
© SPEX SamplePrep, Inc. 2011
Freezer/MillVials & Vial Sets
Micro Small Mid-Size Large
0.1 – 0.5 mL 0.5 – 4.0 mL Up to 25 mL Up to 50 mL
0.1 – 0.5 g 0.5 – 4.0 g Up to 40 g Up to 100 g
Vial Sizes
Vial Materials• Standard Set
• Stainless Steel End Plugs & Impactor with Polycarbonate Center Cylinder• Polycarbonate
• Avoids metal contamination• Stainless Steel
• Best for Polymer applications• Cr-Free
• For RoHS/WEEE appliactions
© SPEX SamplePrep, Inc. 2011
Freezer/MillAuto-Extractor
6814 Auto-Extractor™• Works with Small and Large
size vials• Increases throughput• Reduces operator effort
© SPEX SamplePrep, Inc. 2011
Freezer/MillAccessory Packages
6870S
6870L
6870MIncludes:
• 6754 Small Extractor• 6755 Vial Rack• 6806 Multi-Vial
AdapterIncludes:
• 6884 Mid-Size Adapter for 6804 Large Extractor
• 6886 Mid-Size Grinding Vial Adapter
Includes:
• 6804 Large Extractor• 6805 Vial Rack
© SPEX SamplePrep, Inc. 2011
Freezer/MillLiquid Nitrogen Handling
• Cryogenic Gloves
Liquid Nitrogen• Portable Dewars & Pressurized Tanks
– Required for use with Auto-Fill units(6870 + Auto-Fill & 6970EFM)
Safety
© SPEX SamplePrep, Inc. 2011
Samples, Application Notes & Resources
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Before & After…
Leather
Corn
BoneA Collection of our Before &
After Samples
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Application Notes
• Extensive library of Freezer/Mill® applications
Examples• SP005 – RNA Extraction from Cartilage
Tissue Using Cryogenic Grinding• SP006 – Preparation of Rice Husks for
the Analysis of Their Contents• SP007 – Grinding Polymers For
Qualitative and Quantitative Analysis
Also Available:
Featured Articles Library
© SPEX SamplePrep, Inc. 2011
Product Demonstration Videos