with the financial support of the french ministry of ... · •to overview the problem of...

Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory

Faculty of Veterinary Medicine

Gadjah Mada University

Workshop / Training on

Biotechnology Platform for Research and Training on Parasitic Zoonoses in South East Asia

Yogyakarta, May 19 – 24, 2014

with the financial support of the French Ministry of Foreign Affairs


Trypanosomes and their vectors

• Trypanosoma evansi causes a trypanosomosis (Surra), mechanically transmitted by haematophagous flies, such as Tabanids and Stomoxes.

• The principal host species of T. evansi varies geographically in Indonesia, horses, buffalos and cattle are particularly affected, including wildlife and rodents.

• Human infections have been recently described in India, surra a potential emerging parasitic zoonosis.

• Rodents are also a reservoir of Trypanosoma lewisi, that could be found in Indonesia

Trypanosomiasis in Indonesia

Cattle Trypanosomiasis

Buffalo Trypanosomiasis Cattle and Buffalo Trypanosomiasis

Horse Trypanosomiasis (Cattle also)

Sumatra

Malaysia

West Papua

East Nusa Tenggara

West Nusa Tenggara

Sulawesi

Kalimantan

Yogyakarta

Singapore

Thailand

Malaysia

Philipines

Australia

Java

BioTraps


Vectors and Pathogens Suborder Family Species

Nematocera Culicidae Anopheles sp (malaria vector)

Culex sp (virus vector: Dengue, Arbovirus, etc)

Simuliidae Simulium sp (Onchocercosis)

Ceratopogonidae Culicoides sp (Leucocytozoonosis, Blue tongue)

Psychodidae Phlebotomus sp (Leishmaniasis)

Brachycera Glossinidae Glossina sp (Trypanosomiasis)

Tabanidae Muscidae

Tabanus sp (Trypanosomiasis, Anaplasmosis) Musca sp (bacterial vector) Stomoxys sp (Trypanosomiasis, Dermatophilus)

Family: Muscidae (House-flies and Stable-flies)

• Musca domestica

• Mechanical transmission of pathogens, accidental myiasis.

• Stomoxys calcitrans

• biting pest (human and veterinary pest)


Trypanosoma and Surra in Indonesia

• Many Trypanosomosis outbreaks occur in bovines in Indonesia, and many biting flies as mechanical vector of Trypanosomosis.

• 28 species of Tabanus, 5 Chrysops species and 5 Haematopota sp. were reported as potential vectors of surra in Indonesia

• The high density of stable fly populations can severely decrease livestock productivity.


Outbreak in Sumba Island

• Tabanus and Stomoxys flies has a significant impact in horses and cattle trypanosomosis in Sumba Island in East Nusa Tenggara province of Indonesia during a Surra outbreak in 2012-2013 (393 cases, 350 dead).

• In several Islands in Indonesia, Tabanus rubidus can also transmit zoonotic disease such as Antrax. To date, there is no control method available.


Trypanosomosis in Horses


Good farming system


Garbage farming system

Traditional farming system


Dairy cattle livestock


NZ1 Fly Trap


Fogging

Sticky paper

Herbal fogging Insecticide


Aims of the workshop

• To share knowledge and experience on trypanosomes and their vectors: diagnosis epidemiology, impact and control of the disease as well as trapping, identification, studies of transmission mechanisms, epidemiological impact as vectors and ways of control.

• To strengthen animal health systems approach, focusing on national policy, coordinated at different levels of government to increase technical skills.

• To support the sustainable Indonesia’s veterinary services in Surra erradication according to international standards set by the World Organization for Animal Health (OIE).

• To develope joint research projects amongst French and South East Asian research institutes and universities.


“One Health Open workshop” 19th May 2014

• The workshop will be held under the umbrellas of GREASE and the NAHIAT, in order to strengthen cross-sectorial and multidisciplinary exchanges about trypanosomosis management among professional form different sectors (Animal health, human health, environment, rural development).

• Lectures and open discussions will be held on future collaborations in the One Health (OH) conceptual framework, on trypanosomes and vectors, including livestock, wildlife, environment (both social and natural), veterinary and human medicine

• it will be open to a large audience, especially to represent various geographical areas of Indonesia.


“Registered Workshop” 20th to 24th May 2014

• Limited to 20 participants

• Presentations:

(1) trypanosomes both in humans and animal reservoir in Asia, diagnosis methods and potential control methods, and

(2) their transmission mechanisms and vectors, including trapping, identification and control.

• It will include lectures, field work, practices and laboratory activities


Expected results and outputs

• To overview the problem of trypanosomes in Asia, both in humans and animals including livestock, domestic and wild animal;

• To implement and interpret specific diagnosis of trypanosomoses in mammals, using parasitological, serological and DNA based methods;

• To implement trapping and identification of the main genera/species of biting insects acting as mechanical vectors of trypanosomes in livestock;

• To draft common research project for the control of biting insects within a OH framework

• To build up research methodology for epidemio-surveillance of trypanosomoses.


Time Programme Lecture/Speaker duration

International seminar on Biting Flies and Trypanosomes

09.00 – 09.05 Opening remarks from the committee

Dr. Wisnu Nurcahyo (GMU) 5 min T

09.05 – 09.15 Opening Speech from GMU Prof. Dr. Suratman (Vice Rector Research GMU)

10 min T

09.15 – 09.25 Opening Speech from coordinator of BioZoonoSEA

Prof. Sathaporn Jittapalapong (KU)

10 min T

09.25 – 09.30 Opening Speech from NAHIAT coordinator (CIRAD-IRD)

Dr. Marc Desquesnes (CIRAD / KU)

5 min T

09.30 – 09.45 Coffee break 15 min

Morning Session

09.45 – 10.35 L 1 : One Heath / EcoHealth conceptual framework

Dr. A Binot 50 min T

10.35 – 10.55 L 2 : Parasitic Zoonoses in Thailand Pr. S Jittapalapong 20 min T

10.55 – 11.15 L 3 : VRI and zoonoses in Malaysia Dr C Panchadcharam 20 min T

11.15 – 11.35 L 4 : Atypical Human Trypanosomoses in SEA

Dr S Herder 20 min T

11.35 – 11.55 L 5 : Biting-Insects as mechanical vectors

Dr M Desquesnes 20 min T

11.55 – 12.15 L 6 : Parasitic zoonoses in Indonesia

Dr Wisnu Nurcahyo 20 min T

12.15 – 13.15 Lunch Time (Courtesy of FVM GMU) 60 min


13.15 – 13.30 L 7 : Surra in the Philippines Dr A Dargantes 15 min T

13.30 – 13.45 L 8 : Surra in Malaysia Dr C Panchadcharam 15 min T

13.45 – 14.00 L 9 : Surra in Thailand Pr S Jittapalapong 15 min T

14.00 – 14.15 L 10: Surra in Indonesia Dr April Wardana (Indonesian Veterinary Research Institute)

15 min T

14.15 – 14.30 L 11 : Molecular aspects of surra Pr W Artama 15 min T

14.30 – 14.45 L 12: Economic impact of surra Dr A Dargantes 15 min T

Regional aspects of Surra in Indonesia (90 min) 14.45 – 14.55 L 13 : Surra in Bali Dr. Masa Tenaya

(Bali Diseases Investigation Center) 10 min T

14.55 – 15.05 L 14 : Surra in Java Dr. Fajar Sumping Catur Rasa (Yogyakarta Diseases Investigation Center)

10 min T

15.05 – 15.15 L 15 : Surra in Sumatra Syamsul Maarif, DVM, MSc (Lampung Diseases investigation Center)

10 min T

15.15 – 15.25 L 16 : Surra in Kalimantan Sulaxono Hadi, DVM (Banjarbaru Diseases Investigation center)

10 min T

15.25 – 15.35 L 17 : Surra in Sulawesi Bagoes Purma Jaya, DVM, MSc (Makassar Diseases Investigation Center)

10 min T

15.35– 15.45 L 18 : Surra in Sumba Martono Adi Priyatno, DVM (Veterinarian in East Sumba District)

10 min T


16.00 – 16.15 L 19 : Surra in Regulation of surra in indonesia

Dr. Pujiatmoko (Director of Animal Health)

15 min T

16.15 – 16.25 L 20: GREASE network Dr A Binot 10 min T

16.25 – 16.50 Open Discussion on One Health Concepts : Biting flies and Trypanosomes

Dr A Binot (CIRAD / GREASE) 25 min T

16.50 – 17.00 Closing remarks Dr. A Binot 10 min T

17.00 – 17.05 Announcement Dr. Wisnu Nurcahyo 5 min T


Day 2 : Workshop/Training - Tuesday, May 20th, 2014: Biting flies biology & trapping

Time Programme Lecture/Speaker Place/Time Remark

08.30 – 09.00 30 min

Lecture 1 : An overview on biting insects

Dr Marc Desquesnes T

09.00 – 10.45 105 min

Lecture 2 : Biology of Tabanids and stomoxyine flies



11.00 – 11.30 30 min

Lecture 4 : Seasonality of tabanids in Thailand

Dr Jumnongjit Phasuk T

11.30 – 12.00 30 min

Lecture 5 : Seasonality of stomoxes in Thailand

Dr Jumnongjit Phasuk T

12.00 – 13.00 Lunch Time 60 min

13.00 – 14.15 75 min

Lecture 6 : Trapping technologies



14.30 – 17.30 3 hours

Field trip 1: Setting up of insect traps in the Field (KP4 – GMU)

Dr Marc Desquesnes Dr Jumnongjit Phasuk Dr Wisnu Nurcahyo Dr Alan Dargantes

P

17.30 – 18.00 Back to UC Hotel


Day 3: Workshop/Training - Wednesday, May 21th, 2014: Biting flies trapping & identification

Time Programme Lecture/Speaker Place/Time Remark

08.00 – 10.00 2 hours

Field trip 2: Collect of cages from traps in the field KP4 – UGM

KP4 GMU P


10.15 – 12.00 105 min

Lecture 7: Identification of biting flies: generalities & Tabanids

Dr Marc Desquesnes FVM GMU P

12.00 – 13.00 Lunch Time 60 min FVM GMU

13.00 – 14.45 105 min

Lecture 8: Identification of Stomoxys, Hematobia and Musca crassirostris

Dr Jumnongjit Phasuk FVM GMU T

14.45 – 15.00 Coffee break 15 min FVM GMU

15.00 – 17.00 2 hours

PW1: Identification of biting insects collected in the morning

Dr Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes

FVM GMU P


Day 4: Workshop/Training - Thursday, May 22th, 2014: Biting flies trapping, identification & control;

Trypanosomes: biology & identification

Time Programme Lecture/Speaker Place Remarks

08.00 – 10.00 Field trip 3: Setting up of insect traps in the field of dairy cattle Kaliurang, Sleman

Kaliurang P

10.00 – 10.45 Lecture 9 : Control of biting flies



11.00 – 12.00 Lecture 10 : Biology, morphology and pathogeny of Trypanosomes

Dr Stéphane Herder T

12.00 – 13.00 Lunch Time (lunch box) 60 min FVM GMU

13.00 – 13.30 Lecture 11 : Parasitological diagnosis of Trypanosomes


13.30 – 14.00 Lecture 12 : Serological diagnosis of Trypanosomes



14.15 – 17.00 PW2: Identification of biting insects collected in the morning

Dr Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes

FVM GMU P


Day 5 : Workshop/Training - Friday, May 23th, 2014: Biting flies trapping & identification Trypanosomes: Molecular

diagnosis, characterization and control


08.00 – 10.00 Field trip 4: Setting up of insect traps in the field of beef cattle Glagah Beach, Bantul

Kaliurang P

10.00 – 10.45 Lecture 13: Molecular diagnosis of Trypanosomes

Dr. Wayan Artama FVM GMU


11.00 – 12.00 Lecture 14 : Molecular characterization of Trypanosomes

Dr. Stéphane Herder FVM GMU


13.00 – 13.30 Lecture 15: Control of surra in the Philippines

Dr. A Dargantes FVM GMU P

13.30 – 14.00 Lecture 16: Control of surra in Thailand

Dr. Marc Desquesnes FVM GMU P

14.00 – 16.00 PW3: Identification of biting flies from dairy cattle Kaliurang, Sleman

Dr. Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes

FVM GMU P


16.15 – 17.15 PW4: Practical work in the laboratory for parasitological diagnosis of trypanosomes

Dr. Marc Desquesnes Dr Stéphane Herder Dr Wayan Artama Dr Wisnu Nurcahyo

T


Day 6 : Workshop/Training - Saturday, May 23th, 2014: Biting flies identification Trypanosome molecular diagnosis


08.00 – 12.00 Identification of biting flies from beef cattle Glagah Beach, Bantul

Dr. Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes

FVM GMU P


13.00 – 16.00 PW5: Practical work in the laboratory of Molecular biology for Trypanosomes

Dr Stéphane Herder and Prof. Wayan Artama

FVM GMU P

16.00 – 17.00 Conclusions and Closing ceremony

Dr. Marc Desquesnes Dr. Joko Prastowo

CIRAD/IRD Dean FVM

GMU


No Name of Institution Origin (Province)

1 Faculty of Veterinary Medicine, UGM Yogyakarta

2 Faculty of Veterinary Medicine, University of Udayana Bali

3 Faculty of Veterinary Medicine, Airlangga University, Surabaya East Java

4 Faculty of Veterinary Medicine, Brawijaya University, Malang East Java

5 Faculty of Veterinary Medicine, Nusa Cendana, Kupang East Nusa Tenggara

6 Faculty of Veterinary Medicine, Syiah Kuala Univ, Banda Aceh Banda Aceh

7 Faculty of Veterinary Medicine, NTB Univ Mataram West Nusa Tenggara

8 Faculty of Veterinary Medicine, Wijayakusuma, Surabaya East Java

9 Veterinary Study Programme, Hassanuddin Univ, Makassar South Sulawesi

10 Bogor Agriculture University, Bogor West Java

11 Veterinary Research Institute, Bogor West Java

12 Disease Investigation Center, Yogyakarta Yogyakarta

13 Disease Investigation Center, Denpasar, Bali Bali

14 Disease Investigation Center, Banjarbaru, Kalimantan Kalimantan

15 Disease Investigation Center, Lampung South Sumatra

16 Disease Investigation Center, Bukittinggi, West Sumatra West Sumatra

17 Disease Investigation Center, Medan North Sumatra

18 Disease Investigation Center, Makassar South Sulawesi

19 Faculty of Biology, UGM Yogyakarta

20 Faculty of Medical, UGM Yogyakarta

21 Vector Research Station, Ministry of Health, Salatiga Central Java

22 Rodents Research Station, Banjarnegara Central Java

23 Zoonoses Division, Ministry of Health, Jakarta Jakarta

24 Directorate of Animal Health, Ministry of Agriculture Jakarta


Activities

International Seminar

• Keynote Speakers (CIRAD, GREASE)

• Invited speakers (Asean Country)

Theory and Practical workshop

• Biting flies

• Trypanosomes

Field trips

• Bionomics and habitats of vectors

• Trapping and Field identification


Secretariat: Dept of Parasitology Faculty of Veterinary Medicine Gadjah Mada University Jalan Fauna 2, Karangmalang Yogyakarta 55281 Email: [email protected] [email protected] Website : www.fkh.ugm.ac.id Phone and fax : +62-274-6411525 Mobile : +62-813 25252 7887

mailto:[email protected]





Workshop Thailand


Workshop at NUS Singapore


Workshop @ VRI Malaysia


3/28/2014 Institute for Research and Community

Services, UGM 34

City tour Yogyakarta

Thank you for your attention

See you in Indonesia May 19-25, 2014

with the financial support of the french ministry of ... · •to overview the problem of...

Documents