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Virus Antib(tdies

Envelope e_---------:------,

Envelope glycoproteins e_-------1~ Single-stranded RNA ----,

First identified in 1989, Hepatitis C virus (HCV) affects over 170 million people with almost 3% of the

Nucleocapsid

world population seropositive for anti-HCV antibodies. Chronic infection occurs in 80-85% of those acutely infected and can lead to cirrhosis, liver failure, hepatocellular carcinoma (HCC), and death. HCV belongs to the family Flaviviridae and has a positive-sense, single-stranded RNA genome that codes for a 3011 amino acid polyprotein. This polyprotein is subsequently processed by viral and cellular proteases into three structural proteins (core, E1, and E2) and seven non-structural proteins (p7, NS2, NS3, NS4A, NS48, NS5A, and NS58). While genetic diversity makes HCV highly adaptable to challenges from the host immune system and antiviral drugs, research into HCV biology has revealed new targets (e.g., the NS58 polymerase and the NS3 protease) for specific antiviral therapies that create new hope for HCV-infected people.

GeneTex is proud to offer an outstanding selection of antibodies for HCV research. Please see the highlighted antibodies below or visit our website for a complete list of these gold standard products.

Hepatitis C virus core + NS3 + NS4 antibody (GTX40324) IHC-P analysis of HCV-infected tissue.

MW Huh? (kDa) ----=---:;-lbstrainHCV

130-100-70-55 -40 -35 -

-Hepatitis C virus NS5A protein antibody (GTX131272) WB analysis of NS5A protein in Huh7 cells transfected with 1 b strain HCV replicon.

MW (kDa) 17~ _

19~ = 55 -40 -35-25 -

15 -

Hepatitis C virus Core protein antibody (GTX1 31265) WB analysis of Core protein in Huh7 cells infected with HCV.

MW Huh? (kDa)---.-lbslrain HCV

170 -130-100-70-55-40-

Hepatitis C virus NS5B protein antibody (GTX1 31273) WB analysis of NS5B protein in Huh7 cells transfected with 1 b strain HCV replicon.

MW Huh7 (kDa) -::-;-HCV

170-130 -100 -70 - _ 55-

40-

35 -25 -

Hepatitis C virus NS3 protein antibody (GTX1 31269) WB analysis of NS3 protein in Huh7 cells infected with HCV.

MW Huh? (kDa) ----=---:;-lbslrainHCV

170 -130 -100-70 - -55 - -40-

Hepatitis C virus NS5B protein antibody (GTX131274) WB analysis of NS5B protein in Huh7 cells transfected with 1 b strain HCV replicon.

MW Huh7 (kDa) -::-;-HCV

g~ = 100-70 -55-

40 -

35-25 -

Hepatitis C virus NS3 protein antibody (GTX131276) WB analysis of NS3 protein in Huh7 cells infected with HCV.

Hepatitis C virus antibody (GTX70331) IHC analysis of HCV-infected tissue.

www.genetex.com

Dengue fever is the most serious of the mosquito-borne viral diseases. It is caused by Dengue virus (DENV), from any of the four

serotypes (DEN1-4). Patients develop long-term immunity to the initial infecting serotype. However, sequential infection by different

serotypes leads to a greater risk of serious disease manifestations. DENV is of the genus Flavivirus and possesses a positive-sense

single-strand RNA genome of approximately 10.6 kb that contains a single open-reading frame encoding for a polyprotein, which

can be further cleaved by cellular and viral proteases into three structural proteins (capsid protein C, membrane protein M, envelope

protein E), and seven non-structural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5).

Dengue Virus Protein Schematic Diagram [Non-Structural Proteins (Orange)]

II. ---Capsid prM Envelope NS1 NS2B

(GTX103343) (GTX128093) (GTX629116) (GTX630556) (GTX124246)

kDa 72-55-43-

34-26-

17-

kDa 72- A 8 55-43-34-

26- -17-

kDa A 8

70-55-40-35-25-

15-

10_

kDa 130- A B

100-

70-

55-

40-

35-

25_

-kDa A 8 95-72-55-43-34-

26-

17- _

10-

WB analysis of virus proteins in BHK-21 cells infected with dengue virus. (A) mock. (B) Virus infection.

NS3 NS4A (GTX629477) (GTX124249)

kDa 170- A 8

130-

95-

72-

55-

kDa 130- A 8 72-55-43-34-26-

17-

10-

NS4B (GTX124250)

kDa 130- A 8

95-72-

55-

43-

34-

26-

NS5 (GTX629447)

130-100-70-

55-

40-

35-

25_

A 8

- Featured Products

Dengue 1 virus infection Dengue 2 virus infection

Dengue virus 1, 2, 3 &4 antibody [01-11(3)] (GTX29202) ICC/IF analysis of dengue virus proteins in BHK-21 cells infected with the indicated dengue viruses.

Mock Dengue 2 virus infected

I

I

I

I

I

I

NS1 (Dengue virus) antibody [GT1061] (GTX630557) ICC/IF anaylsis of NS1 protein in BHK-21 cells infected with dengue 2 virus.

NS4B (Dengue virus) antibody (GTX103349) ICC/IF anaylsis of NS4B protein in BHK-21 cells infected with dengue 2 virus.

N 0'> o 00 N .-<

~ \!)

PrM (Dengue virus) antibody (GTX128092) ICC/IF anaylsis of prM protein in BHK-21 cells infected with dengue 2 virus.

20~m

20f-lm

Dengue 3 virus infection Dengue 4 virus infection


NS3 (Dengue virus) antibody [GT2811] (GTX629477) ICC/IF anaylsis of NS3 protein in BHK-21 cells infected with dengue 2 virus.

r-.-< .-< 0'> N -0 X l\!)


Envelope Protein (Dengue virus) antibody [GT643] (GTX629117)

ICC/IF anaylsis of NS3 protein in BHK-21 cells infected with dengue 2 virus.


Capsid Protein (Dengue virus) antibody (GTX124247) ICC/IF anaylsis of capsid protein in BHK-21 cells infected with dengue 3 virus.

~Japanese encephalitis virus (JEV)

Japanese encephalitis is a very serious infectious disease caused by Japanese encephalitis virus (JEV), which belongs to the

family Flaviviridae and is maintained in a zoonotic cycle involving the Culex genus of mosquitoes and vertebrate hosts. Endemic to

most of Asia and to certain regions of the Western Pacific, JEV frequently causes severe neurologic sequelae or death in infected

humans. This enveloped virus possesses a positive-sense single-stranded RNA genome packaged in a spherical nucleocapsid.

The genome contains a single open reading frame that encodes for a polyprotein, which is further cleaved into three structural (C,

prM, E) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5).

s'cap __ '·J111 3'

\~----------~r-----------~L------------------------'r-------------------------~) V V Structural Non-Structural

_ List of Representative Products

-~ . .. t:l1l':OOl . . . . ,~ .. •

GTX131368 Core protein C (JEV) antibody Rb pAb JEV WB, ICCIIF

GTX131833 PrM (JEV) antibody Rb pAb JEV WB,ICCIIF

GTX125867 envelope protein (JEV) antibody Rb pAb JEV ICC/IF, IHC-P, WB

GTX131369 NS1 (JEV) antibody Rb pAb JEV ICC/IF, WB

GTX125972 NS2B (JEV) antibody Rb pAb JEV ICC/IF, WB

GTX125868 NS3 (JEV) antibody Rb pAb JEV ICC/IF, WB

GTX125865 NS4B (JEV) antibody Rb pAb JEV ICC/IF, WB

GTX131359 NS5 (JEV) antibody Rb pAb JEV WB,ICCIIF

GTX131360 NS5 (JEV) antibody Rb pAb JEV WB, ICCIIF

II JEV

- Featured Products

Core protein C (JEV) antibody (GTX131368) ICC/IF analysis of Core protein C in BHK-21 cells infected with JEV.

NS1 (JEV) antibody (GTX131369) ICC/IF analysis of NS1 protein in BHK-21 cells infected with JEV.


MW BHK-21 (kDa) --::-;-JEV

170_ 130-100-70-55 -

40-

35-

25-

non-structural _ protein NS1 ' (JEV) ... non-structural

protein NS1 (JEV)

MW BHK·21 (kDa) --::-;-JEV

1~~ = ~8 : 35-25-

15 -

10-

PrM protein (JEV)

NS1 (JEV) antibody (GTX131369) WB analysis ofNS1 protein in BHK-21 cells infected with JEV.

PrM protein (JEV) antibody (GTX131833) WB analysis of PrM protein in BHK-21 cells infected with JEV.

Qyality Antibodies

~GeneTex www.genetex.com

n~(. a~ ona Tel 886.3.6208988 Fax 886.3.6209098


Envelope protein (JEV) antibody (GTX125867) ICC/IF analysis of envelope protein in BHK-21 cells infected with JEV


~ ~JF¢"",f' 130-100-70 -

55 -

40-

35 -

25-

Envelope protein (JEV) antibody (GTX125867) WB analysis of envelope protein in the BHK-21 cells infected with the indicated viruses.

~ ~"'~q:¢ 72-55-43-

34 -

26-

17 -

10 -

NS4B (JEV) antibody (GTX125865) WB analysis of NS4B protein in the BHK-21 cells infected with the indicated viruses.

• Qyality Results

USA Toll-free 1.877.GeneTex(1 .877.436.3839) Fax 1.949 .309.2888 ~~

Address 6F-2 , No.89, Dongmei Rd ., Hsinchu 300 , Taiwa n (R.O.C.) Address 2456 Alton Parkway, Irvi ne, CA 92606 USA ~~~ E-mail [email protected] E-mail [email protected] www.genetex.com

www.genetex.com

.-VPl

.- VP2

.- VP3

.- VP4

5'UTR PI P2 P3 3'UTR

3D AM

o 1000 2000 3000 4000 5000 6000 7000

Genome length in nucleotides

Discovered in 1969, human enterovirus 71 (EV71) is the causative agent of hand-foot-and-mouth disease (HFMD) in young children,

with a subset of patients developing more severe neurological disease that can result in death. As a member of the genus Enterovirus

in the family Picornaviridae, EV71 has a positive-sense single-strand RNA genome coding for a 2,194 amino acid polyprotein, which

is subsequently processed into four structural (VP1-VP4) and seven non-structural (2A, 28, 2C, 3A, 38, 3C, 3D) proteins. Identifying

the specific functions of these proteins and their interactions with host factors will provide crucial information regarding

EV71-associated neural pathogenesis and viral replication.

GeneTex is proud to offer an outstanding selection of antibodies for EV71 research. Please see the highlighted antibodies below or

visit our website to see latest EV71 research products.

MW ,CY,oJ (kDa) -"J(J

1~~= 55 -40-35-25-

EV71 antibody (GTX124261) WB analysis of EV71 proteins in RD cells infected with EV71 virus.

1~~~ 35-25-

15-

EV71 VP1 antibody (GTX132338) WB analysis of EV71 VP1 protein in RD cells infected with EV71 virus.

~ _ "J' 70 -55-40-35-

25 --<

15-

10 -

EV71 3C antibody [B2] (GTX630656) WB analysis of EV71 3C protein in RD cells infected with EV71 .

1~~= 40 -35-25 -

15-

VPO VP2

EV71 VP2 antibody (GTX132340) WB analysis of EV71 VP2 protein in RD cells infected with EV71 virus.

GeneTex International USA

Tel: 886.3.6208988 Tel: 1.949.553.1900

~ - "J' 70 -55-40-

35-

25-

15-

10-

EV71 3C antibody [B3] (GTX630191) WB analysis of EV71 3C protein in RD cells infected with EV71 .

MW ,CY (kDa) - "J

15-

10-

EV71 Protease 2B antibody (GTX132343) WB analysis of EV71 2B protein in RD cells infected with EV71 virus.

Toll-free: 1.877.436.3839

~ "J' 170- -

130-

100 -

70-

55-

40 -

EV71 3D antibody [4] (GTX630193) WB analysis of EV71 3D protein in RD cells infected with EV71 .

MW __ <l'.ct,oJ (kDa) 0/ 0/

15-

10-

EV71 Protease 3AB antibody (GTX132344) WB analysis of EV71 3AB protein in RD cells infected with EV71 virus.

PD-V001-150803

II ~- "

" .. ~ ,"

Influenza Virus

- Featured Products - Influenza A

FluA NS1 DAPI WGA Merge

Mock

Infection

Influenza A Virus NS1 antibody (GTX125990) ICC/IF analysis of NS1 protein in Vero cells infected with infiuenza A virus (WSN).

mock

WSN infection

FluA HA DAPI Merge

Influenza A Virus H1N1 Hemagglutinin (HA) antibody [GT223] (GTX629750) ICC/IF analysis of H1 N1 HA protein in OF1 cells infected with influenza A virus (WSN).

k~~ _ '"c~ ""~"* 55-43-34-26-

17 -

10 -

Influenza A Virus M1 antibody (GTX125928) WB analysis of M1 protein in OF-1 cells infected with infiuenza A virus (WSN) .

~ ~ 72- cP~ «..'~ 55-43-34 -26-

17-

10-

Influenza A Virus M2 antibody (GTX125951) WB analysis of M2 protein in OF-1 cells infected with infiuenza A virus (WSN) .

FluA NP DAPI WGA Merge

Mock

Infection

Influenza A Virus NP antibody (GTX125989) ICC/IF analysis of NP protein in Vero cells infected with influenza A virus (WSN).

mock

WSN infection

FluA NP DAPI Merge

Influenza A Virus NP antibody [GT1236] (GTX629633) ICC/IF analysis of NP protein in OF1 cells infected with infiuenza A virus (WSN) .

k1~~_ '"c~ i~ 130-

95-

72-

55-

43-

Influenza A Virus NP antibody [GT1236] (GTX629633) WB analysis of NP protein in OF-1 cells infected with influenza A virus (WSN).

kDa ~_""""",, 130- '"c'v

1~ = 55-43-34-

26-

17-10-

Influenza A Virus NS1 antibody (GTX125990) WB analysis of NS1 protein in OF-1 cells infected with infiuenza A virus (WSN) .

-

-

There are three types of influenza virus: Types A, B, and C. The influenza A and B viruses are

the ones most associated with serious human infections, while the Type C cases are clinically

much milder. The main antigenic determinants of influenza A and B viruses are the

haemagglutinin (HA) and neuraminidase (NA) transmembrane glycoproteins. Based on the

antigenicity of these glycoproteins, influenza A viruses are further subdivided into sixteen

"H" (H1-H16) and nine "N" (N1-N9) subtypes. The influenza A virus genome consists of

eight separate RNA segments that encode the proteins (i.e., PB1, PB2, PA, HA, NA,

NP, M1, M2, NS1, and NS2) essential for host infection, viral genome replication, and

virus particle packaging.

Featured Products - Influenza B

mock

fluB infection

FluB M1 DAPI Merge

mock

fl uB infection

FluB NA DAPI Merge

Influenza B Virus M antibody (GTX128537) Influenza B Virus Neuraminidase (NA) antibody (GTX128540) ICC/IF analysis of M protein in MDCK cells infected with influenza B virus (Taiwan B70555).

mock

fluB infection

FluB HA DAPI Merge

Influenza B Virus Hemagglutinin (HA) antibody (GTX128542) ICC/IF analysis of HA protein in MDCK cells infected with influenza B virus (Taiwan B70555).

H-N Antigenicity-specific Antibodies

Avian Human H1 H3 H5 H7 H9 H1 H1 H3

170_ 130 -95-

~~ = . • 43-34 -

25-

12345678

FluAH1N1 HA(GTX127295)


17~ _ 1~5 = 72-55- _

43-

34-

25-

12345678

FluA H5N3 HA (GTX127299)

ICC/IF analysis of NA protein in MDCK cells infected with influenza B virus (Taiwan B70555).

GTX629882

coslained with Hoechst 33342

Mock Influenza B Virus infecled

- , . .. I ..

Influenza B Virus NP antibody [GT371] (GTX629882) ICC/IF analysis of NP protein in MDCK cells infected with influenza B virus (Taiwan B70555).

170-1~~ = 72-55 -43-

34-

25-


12345678


170_ 130-

95 -


~~ = -43-

34-

25-

1234567


WB analysis in MDCK ceillysates infected with different subtypes of human and avian influenza virus A by using influenza A Virus H1N1 HA antibody (GTX127295) . influenza A Virus H5N3 HA antibody (GTX127299). influenza A virus H7N7 HA antibody (GTX127303), and influenza A virus H9N2 HA antibody (GTX127305).

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