your recovery sensitivity before analysis! sults driven by · recovery before analysis! ... dia...
TRANSCRIPT
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SULTS DRIVEN BY:Easily improve your sensitivity and recovery before analysis!With the widest range of sample preparation solutions from Phenomenex you can clean up any matrix before testing for PAHs!
roQ Type: QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe)Description: Quick and efficient process to extract multi-residue target analytes from food samples while removing un-wanted interferences such as organic acids, lipids, pigments, sugars, and more. Each kit contain centrifuge tubes, pre-weighed salts in convenient sin-gle-use packets, and pre-weighed and packed SPE centrifuge tubes.
Strata-X Polymeric SPEType: Solid Phase ExtractionDescription: Unique, combined reversed phase and ion-exchange polymeric me-dia developed to cover a diverse spec-trum of analytes and simplify the meth-od development process for fast and efficient sample preparation. Available in tubes (ranging from 1 mL to 60 mL) and standard and microelution 96-well plate formats.
Strata Silica SPEType: Solid Phase ExtractionDescription: Reversed phase, normal phase, or ion-exchange based media that allows for direct retention and elu-tion of compounds of interest, with wash steps to remove unwanted interferenc-es. Available in tubes (ranging from 1 mL to 60 mL) and 96-well plate formats. Strata PAH is available for clean up of water samples.
Polymeric SPE
Solid Phase Extraction
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Continued Innovation with GC ExpertiseOur inventive GC scientists have 25+ years of experience, and many helped create keystone phases at J&W Scientific before joining the Phenomenex team. Zebron’s GC column track record of innovation is the only one to be recognized with 3 R&D 100 Awards, 2 Readers’ Choice Awards, and a Pittcon Excellence Award. This tradition of GC column inno-vation continued with ZB-DHA-PONA, and continues with the NEW Zebron ZB-PAH-EU and Zebron ZB-PAH-SeleCT.
2005 2006 2009 20101998
Birth of Zebron GC Columns
ZB-WAXplus™
100 % water stableZB-1XT SimDist
Robust simulated distillation
2004
MS PhasesLow bleed,
sensitive performance
ZB-MultiResidue™ PhasesTargeted selectivity for
pesticide screening
ZB-PAH-EU ZB-PAH-SElECT
2007
Inferno™ PhasesStable up to 430 °C
ZB-Drug-1For drugs of abuse
ZB-Bioethanol5 min results
2012
ZB-SemiVolatilesSupreme inertness for environmental SVOCs, PBDEs, and PAHs
ZB-CLPesticides PhasesVersatility for pesticides by
GC/ECD
2014
ZB-5MSplus™
Versatile, inert performance for
industrial and food applications
2015
ZB-624plus™
The Next Generation of GC Inertness.
Pittcon Excellence Award
2018 2020
ZB-FAMEFaster results and
improved separation of cis/trans FAME
isomers
2017
SULTS DRIVEN BY:
GC
Zebron™ Gas Chromatography Technology
More Than Just A ColumnWhen you choose Zebron, you get more than just high quality GC products. Choos-ing Zebron means you get access to a wide variety of tools, resources, and personalized support to help make your GC work easier, faster, and simply better.
PHASE CHEMISTRY
DEACTIVATION
FUSED SILICA
ZB-DHA-PONADesigned for the detailed
hydrocarbons analysis
2019
Zebron Gas Management Filter and Trap
The ultimate protection for your GC column and
instrument
Phenomenex l WEB: www.phenomenex.com
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SULTS DRIVEN BY:
www.phenomenex.com/PAH www.phenomenex.com/PAH www.phenomenex.com/PAHFind more methods and applications online at [email protected] ww
Find more methods and applications online at www.phenomenex.com/PAH
Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.Trademarks Kinetex and Strata are registered trademarks and Zebron, Inferno, MultiResidue, WAXplus, 5MSplus, 624plus, Z-Liner, MidBore, roQ, SecurityGuard, and BE-HAPPY are trademarks of Phenomenex. Shimadzu is a registered trademark of Shimadzu Corporation. Strata-X is patented by Phenomenex. U.S. Patent No. 7,119,145.
FOR RESEARCH USE ONLY. Not for use in clinical diagnostic procedures.© 2020 Phenomenex, Inc. All rights reserved.
Analysis Priority
Find more methods and applications online at
www.phenomenex.com/PAH
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USAt: +1 (310) 212-0555
All other countries/regions Corporate Office USA
t: +1 (310) 212-0555 [email protected]
www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country/region, contact Phenomenex USA, International Department at [email protected]
Phenomenex l WEB: www.phenomenex.com
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Kinetex PAH • Expanded resolution with chemical
selectivity specifically for PAHs
• Increased throughput and sensitivity with core-shell technology for HPLC/UHPLC
Zebron ZB-PAH-EU • Up to 70% Faster PAH Analysis
• Elevated Temperature Stability (340/360 °C)
Zebron ZB-PAH-SeleCT • Enhanced Resolution for Chrysene
and Triphenylene (PAH interferences)
• Elevated Temperature Stability (320/340 °C)
GC Columns
Core-Shell Technology
LC GC
Analysis Priority
Core-Shell Technology
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The Chosen Core-Shell Brand Kinetex® Core-Shell Technology delivers dramatic im-provements in efficiency over conventional fully porous media which can be leveraged to increase resolution, greatly improve productivity, reduce solvent consump-tion, and decrease costs. Whether you are running HPLC or UHPLC methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using.
Finely Tuned, Unique Core-Shell Manufacturing ProcessPhenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles. Using silica sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a core-shell particle. The combination of a consistent, solid high density core along with proprietary column packing technologies ensures optimum bed structure and high column performance.
Typical Core-Shell Performance Gains!
Kinetex Core-Shell Technology
Solid Core Porous Shell
You can unleash the power of Kinetex Core-Shell Tech-nology columns to achieve faster and better results just not possible on conventional fully porous materials. No matter what type of system you have or type of analysis you are performing, there is a Kinetex solution for you.
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FASTER, BETTER RE
© 2020 Phenomenex, Inc. All rights reserved.
Phenomenex l WEB: www.phenomenex.com
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Designed for
Polycyclic aromatic hydrocarbons (PAHs) are a haz-ardous set of compounds that come from a variety of materials and processes. With the need to accurately identify toxic forms of these compounds quickly in the environmental, fuel, and food industries, we specifically developed the first core-shell based LC product for PAH analysis. Now high resolution PAH separations can be attained under fast run times on both HPLC or UHPLC instrumentation.
Controlled pore size processing and a proprietary polymerically bonded stationary phase were devel-oped to ensure excellent resolution between priority polycyclic aromatic hydrocarbons (PAHs). Combined with core-shell particle technology, incredibly high efficiency and sensitivity at comfortable LC pressures is very achievable.
Have either an HPLC or UHPLC?No worries! The Kinetex® PAH 3.5 µm core-shell particle size and format has been chosen to provide optimal performance on both HPLC and UHPLC in-strumentation.
PAH Analysis by HPLC/UHPLC
Learn More OnlineFind more methods and applications online at www.phenomenex.com/PAH
PAH
O
SiO
Si
O
SiO
Si
Innovative Polymerically Bonded C18 Core-Shell PAH Column!
“”
• Enhanced resolution and performance for PAH analysis
• QC tested for PAHs (EPA 610)
Phenomenex l WEB: www.phenomenex.com
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EPA and EU
EPA 610 – PAH Analysis
EPA 8310 – PAH Analysis
EU 15+1 PAH Analysis
LC Application Examples
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Column: Kinetex 3.5 µm PAHDimensions: 100 x 4.6 mm
Part No.: 00D-4764-E0Mobile Phase: A: Water
B: Acetonitrile Gradient: Time (min)
07
% B50100
8 1009 5012 50
Flow Rate: 1.2 mL/min Backpressure: 136 BarTemperature: 35 ˚C
Detection: UV @ 292 nmSample: 1. Naphthalene
2. Acenaphthylene3. 1-Methylnaphthalene4. 2-Methylnaphthalene5. Acenaphthene6. Fluorene7. Phenanthrene8. Anthracene9. Fluoranthene
10. Pyrene11. Benz[a]anthracene12. Chrysene13. Benzo[b]fluoranthene14. Benzo[k]fluoranthene15. Benzo[a]pyrene16. Dibenz[a,h]anthracene17. Benzo[g,h,i]perylene18. Indeno[1,2,3-cd]pyrene
Column: Kinetex 3.5 µm PAHDimensions: 100 x 4.6 mm
Part No.: 00D-4764-E0Mobile Phase: A: Water
B: Acetonitrile Gradient: Time (min)
07
% B50100
8 1009 5012 50
Flow Rate: 1.2 mL/min Backpressure: 136 BarTemperature: 35 ˚C
Detection: UV @ 292 nmSample: 1. Naphthalene
2. Acenaphthylene 3. Acenaphthene 4. Fluorene 5. Phenanthrene 6. Anthracene 7. Fluoranthene 8. Pyrene
9. Benz[a]anthracene10. Chrysene 11. Benzo[b]fluoranthene 12. Benzo[k]fluoranthene 13. Benzo[a]pyrene 14. Dibenz[a,h]anthracene 15. Benzo[g,h,i,]perylene 16. Indeno[1,2,3-cd]pyrene
Column: Kinetex 3.5 µm PAHDimensions: 100 x 4.6 mm
Part No.: 00D-4764-E0Mobile Phase: A: Water
B: Acetonitrile Gradient: Time (min)
06
% B50100
11.5 10012 5014 50
Flow Rate: 1.5 mL/min Backpressure: 136 BarTemperature: 35 ˚C
Detection: UV @ 292 nmSample: 1. Benzo[c]fluorene
2. Cyclopenta[cd]pyrene3. Benz[a]anthracene4. Chrysene5. 5-Methylchrysene6. Benzo[j]fluoranthene7. Benzo[b]fluoranthene8. Benzo[k]fluoranthene
9. Benzo[a]pyrene10. Dibenzo[a,l]pyrene11. Dibenz[a,h]anthracene12. Benzo[g,h,i]perylene13. Indeno[1,2,3-cd]pyrene14. Dibenzo[a,e]pyrene15. Dibenzo[a,i]pyrene16. Dibenzo[a,h]pyrene
Core-Shell Technology
Phenomenex l WEB: www.phenomenex.com
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Complete Resolution of EU 15+1 and EPA 610 PAHsZebron ZB-EU-PAH GC column demonstrates excellent resolution and accurate quantitation of European regulated EU 15+1 and EPA 610 PAHs.
Analysis of EU 15+1 PAHs
Analysis of EU 15+1 and EPA 610 PAHs
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Ap
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Excellent Resolution of EU PAH Critical Pairs
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Sample: 1. Benzo[c]fluorene 2. Benz[a]anthracene 3. Cyclopenta[c,d]pyrene 4. Chrysene 5. 5-Methylchrysene 6. Benzo[b]fluoranthene 7. Benzo[k]fluoranthene 8. Benzo[j]fluoranthene9. Benzo[a]pyrene
10. Indeno[1,2,3-cd]pyrene 11. Dibenz[a,h]anthracene 12. Benzo[g,h,i]perylene 13. Dibenzo[a,l]pyrene 14. Dibenzo[a,e]pyrene 15. Dibenzo[a,i]pyrene 16. Dibenzo[a,h]pyrene
GC-MS conditions for both applications:Column: Zebron ZB-PAH-EU
Dimensions: 30 meter x 0.25 mm x 0.20 µmPart No.: 7HG-G043-10
Injection: Split 5:1 @ 330 °C, 1 μL Recommended Liner: Zebron PLUS Single Taper Z-Liner™
Liner Part No.: AG2-4B13-05 (for Shimadzu® 2010 GC)Carrier Gas: Helium @ 24 psi (constant pressure)
Oven Program: 45 °C for 0.8 min to 200 °C @ 45 °C/min to 226 °C @ 3 °C/min for 0 min to 320 °C @ 10 °C/min for 20 min
Detector: MSD, 50-500 m/zTransfer Line Temperature: 300 °C
Source Temperature: 300 °C
1. Naphthalene 2. Acenaphthylene 3. Acenaphthene 4. Fluorene 5. Phenanthrene 6. Anthracene 7. Fluoranthene8. Pyrene 9. Benzo[c]fluorene
10. Benz[a]anthracene 11. Cyclopenta[c,d]pyrene 12. Chrysene 13. 5-Methylchrysene 14. Benzo[b]fluoranthene 15. Benzo[k]fluoranthene 16. Benzo[j]fluoranthene 17. Benzo[a]pyrene 18. Indeno[1,2,3-cd]pyrene
19. Dibenz[a,h]anthracene 20. Benzo[g,h,i]perylene 21. Dibenzo[a,l]pyrene 22. Dibenzo[a,e]pyrene 23. Dibenzo[a,i]pyrene 24. Dibenzo[a,h]pyrene
Sample:
Great Separation of All Key PAH Isomers
Rela
tive
Abu
ndan
ceRe
lativ
e A
bund
ance
Phenomenex l WEB: www.phenomenex.com Phenomenex l WEB: www.phenomenex.com
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GC ColumnsExcellent Resolution of Chrysene and TriphenyleneWe designed the Zebron ZB-PAH-SeleCT GC column to achieve complete resolution of Chrysene from Triphenylene along with other EU 15+1 PAH compounds. Its unique selectivity helps eliminate false positives while resolving PAH isomers, providing easy, fast, and accurate quantification of PAHs in environmental and food samples.
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Ap
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EU 15+1 PAH Analysis Using Zebron ZB-PAH-SeleCT GC Column
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Column: Zebron ZB-PAH-SeleCTDimensions: 40 meter x 0.18 mm x 0.14 µm
Part No.: 7PD-G044-47Injection: Split 30:1 @ 320 °C, 1 μL
Recommended Liner: Zebron PLUS Single Taper Z-Liner™
Liner Part No.: AG2-4B13-05 (for Shimadzu® 2010 GC)Carrier Gas: Helium @ 78 psi (constant pressure)
Oven Program: 45 °C for 0.8 min to 200 °C @ 45 °C/min to 265 °C @ 3 °C/ min for 5 min to 270 °C @ 1 °C/min to 320 °C @ 10 °C/min for 15 min
Detector: MSD (Shimadzu GC-MS-QP2010 Ultra)Mode: SIM
SIM Ions: 216, 226, 228, 242, 252, 276, 278, 302 m/zTransfer Line Temperature: 300 °C
Source Temperature: 300 °C
Sample:1. Benzo[c]fluorene2. Cyclopenta[c,d]pyrene3. Benz[a]anthracene4. Triphenylene5. Chrysene6. 5-Methylchrysene7. Benzo[b]fluoranthene8. Benzo[j]fluoranthene9. Benzo[k]fluoranthene
10. Benzo[a]pyrene11. Indeno[1,2,3-c,d]pyrene12. Dibenz[a,h]anthracene13. Benzo[g,h,i]perylene14. Dibenzo[a,l]pyrene15. Dibenzo[a,e]pyrene16. Dibenzo[a,i]pyrene17. Dibenzo[a,h]pyrene
Separation of Benzo[b,j,k]
Complete Separation ofTriphenylene and Chrysene
Phenomenex l WEB: www.phenomenex.com
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Ordering Information
Sample Preparation Find part numbers, phases and sizes for our sample preparation solutions through the following links:
roQ™ QuEChERS: www.phenomenex.com/roQ
Strata® Silica SPE www.phenomenex.com/strata
Strata-X Polymeric SPE www.phenomenex.com/strata-x
Kinetex®
3.5 μm Minibore Columns (mm)SecurityGuard™ULTRA
Cartridges (mm)Phase 50 x 2.1 100 x 2.1 150 x 2.1 3/pk
PAH 00B-4764-AN 00D-4764-AN 00F-4764-AN AJ0-9535for 2.1 mm ID
3.5 μm MidBore™ (mm)SecurityGuard ULTRA
Cartridges (mm)Phase 100 x 3.0 3/pk
PAH 00D-4764-Y0 AJ0-9534for 3.0 mm ID
3.5 μm Analytical Columns (mm)SecurityGuard ULTRA
Cartridges (mm)Phase 100 x 4.6 150 x 4.6 250 x 4.6 3/pk
PAH 00D-4764-E0 00F-4764-E0 00G-4764-E0 AJ0-9533for 4.6 mm ID
*SecurityGuard ULTRA Cartridges require holder, Part No.: AJ0-9000
Your happiness is our mission. Take45 days to try our products. If you are not happy, we’ll make it right.
guarantee www.phenomenex.com/behappy
Zebron™ ZB-PAH-EU GC Column Length (meter) ID (mm) df (µm) Temp. Limits (°C) Part No.
10 0.10 0.08 40 to 340/360 7CB-G043-5920 0.18 0.14 40 to 340/360 7FD-G043-4730 0.25 0.20 40 to 340/360 7HG-G043-1060 0.25 0.20 40 to 340/360 7KG-G043-10
Zebron ZB-PAH-SeleCT GC ColumnLength (meter) ID (mm) df (µm) Temp. Limits (°C) Part No.
20 0.18 0.14 40 to 320/340 7FD-G044-4730 0.25 0.20 40 to 320/340 7HG-G044-1040 0.18 0.14 40 to 320/340 7PD-G044-47
Phenomenex l WEB: www.phenomenex.com