zh jzhenjun sun china agricultural university beijing...

Cyprus conference, 2016

Vermicomposting agricultural waste into value-p g gadded products: 30yr’s R & D in China

Zh j SUNZhenjun SUN

China Agricultural University Beijing Vermitech Institute

Email：[email protected]

College of Resources and Environmental Sciences, CAU

@

Cyprus conference, 2016The Status of Agricultural Waste Utilization in China

Total agricultural waste resources：1 billion tons, includingg , gCrop straws: 0.7 billion tons; livestock manures: 0.18 billion tons

Manure fertilizer10%

Directly returning to field10% returning to field

50%

Bi

Directly returning to field

Burning 40%

Biogas production 3%

returning to field20%

strawsmanures

elop

ed

reaIndustrial

Organic fertilizer 5%

manures

U t t d 40D

eve armaterials 3%

Surplus 30-40% Surplus 70 Environmental

Untreated 40-45%


Surplus 70-80%

Environmental pollution


A i lt l Ci l E D l t P tt

Coordination

Agricultural Circular Economy Development Pattern

Agr Small Conventional Live Resource

Coordination

ricultu

Internal circulation

Circular

agriculture

Ecological Production Environmentural w

L t l

Circular agriculture

gagriculture

Agriculture and

ecology

Life

Society

Economy

waste

Large external circulation

Agriculture andindustry combination

Life Economy

Circular societyCollege of Resources and Environmental Sciences, CAU

Circular society

Cyprus conference, 2016 Use strategy

MethodsProblems ResultsMethodsProblems Results

LLarge Quantity Efficient Quantity

Poor Quality

Highquality

QualityQuality

Low value Industrial Value

Harmful Recycle Harm



Ecological UtilizationStraw returning

Methane productionMethane production

Edible fungig

Vermicomposting

Agriculture and livestock

composting

Multiple integrationCollege of Resources and Environmental Sciences, CAU

Multiple integration


Hi h Q lit UHigh Quality Use

Commercialization and Marketization

New products：

Bi l i l i （Bi l i l f iliBiological preparation（Biological fertilizer、

Biological pesticides Etc ）Biological pesticides Etc.）

Biochemical preparation（Amino acidsBiochemical preparation（Amino acids、

Enzyme、Oligosaccharides Etc.）College of Resources and Environmental Sciences, CAU

Enzyme、Oligosaccharides Etc.）

Cyprus conference, 2016产品

15000

16000

产品

12000

14000

60008000

10000

750

4000

6000

150 300750

0

2000

原料传统堆肥商品有机肥叶面肥或酶制剂木寡糖或抗菌肽原料传统堆肥商品有机肥叶面肥或酶制剂木寡糖或抗菌肽

Comparison of the added value of the products developedComparison of the added value of the products developed from agricultural wastefrom agricultural waste

College of Resources and Environmental Sciences, CAUComparison of the added value of the products developedComparison of the added value of the products developed from agricultural waste from agricultural waste


Vermiculture in China



f ff i i l i ldfactors affecting vermiculture yield thi kmaterials thickness density

residuals→ earthworm→

Vermiculture

earthworm→ vermicast→

harvestconditions Bed design


Cyprus conference, 2016Simple windrow system p y

大田养殖大田养殖



Plastic Greenhouse System in North China



Composting pretreatment



Intercroping system:p g yvermiculture in field



College of Resources and Environmental Sciences, CAUIntercropping of vermicomposting and banana


A ib i l id i h Ei i F idAntibacterial peptides in earthworm Eisenia FetidaSun (1995) found an antibacterial peptide that was composed of

f i id f h fi i h ldforty amino acids from earthworm at first in the world. Name Sequence of amino

acidNumber of amino acid

Molecular weight

Antibacterial characteristicacid amino acid weight characteristic

EP1 / 40 4832 Bacteria and fungi resistance

EP2 Ac-Ala-Met-Val-Ser-Ser 6 535.27 Bacteria resistance

EP3 Ac-Ala-Met-Val-Gly-Thr 6 519.27 Bacteria resistancey

EP4 / / About 20000

Bacteria resistance

ECP5-1 Ala-Cys-Ser-Ala-Gly 5 510.80 Bacteria resistance

College of Resources and Environmental Sciences, CAUThe methods of earthworm peptide separation and purification have achieved the patent in 2004.


Sequence of amino acid in ECP5-1

College of Resources and Environmental Sciences, CAUAla-Cys-Ser-Ala-Gly

Cyprus conference, 2016Influence of peptide on cancer cells

Th i f EP d EP MGC803 llThe resistance of EP2 and EP3 to MGC803 cell

Scanning electron microsoopical observation on MGC803 Scanning electron microsoopical observation onScanning electron microsoopical observation on MGC803 cells which was untreated with antibacterial peptides

Scanning electron microsoopical observation on MGC803 cells treated with EP2 for 24h

Scanning electron microsoopical observation S i l t i i l b ti





The character of earthworm antibacterial peptides

●The antibacterial peptide had stable physicochemical properties such as heat stability and wide pH scopeproperties such as heat stability and wide pH scope. There was little influence of pH 3, 5, 7, 9, 11 on the antibacterial activities of antibacterial tetradecapeptideantibacterial activities of antibacterial tetradecapeptide. And the antibacterial tetradecapeptide could keep when storing 180 days in home temperaturestoring 180 days in home temperature.

● The antibacterial mechanism and character of the five earthworm peptides was also researched in our group .



Influence of Escherichia coli on the antibacterial function from Eisenia fetida

We have a new finding that fat from earthworm have theWe have a new finding that fat from earthworm have the function of bacteria resistance.


Cyprus conference, 2016Vermicasts as function fertilizer

College of Resources and Environmental Sciences, CAUThe resistance of vermicompost to anthracic of cucumber


Two function microbes from vermicompost

0103A (Streptomyces globisporus）

0104A (Streptomyces syringini）( y y g ）

Fibrillae of 0103A spores Spores of 0103A Spores of 0104A

Fibrillae of 0104A spores



Bioreactor for waste conversionCollege of Resources and Environmental Sciences, CAU

Bioreactor for waste conversion A Chinese patent

Cyprus conference, 2016Function liquid fertilizer

Earthworm ProductsCollege of Resources and Environmental Sciences, CAU

Earthworm Products


Earthworm ProductsCollege of Resources and Environmental Sciences, CAU

Earthworm Products


Some products developed in CAU

① products processed from earthworm● d f l● amino acid for animal nutrition● foliar fertilizer with amino acid● amino acid biopestcide

② e i o o t② vermicompost：● commercial fertilizers

● soil less culture medium



amino acid processing from earthworm

Worm →hydrolyzing with enzyme → raw amino acid→ adding microelement→ raw amino acid → adding microelement

→AA additives → chelating → chelated AA →AA foliar fertilizerg

→AA pesticides



amino acid products: biochemical fertilizer



Micro‐ecological adjustant and function fertilizerfunction fertilizer

① Some effective antagomistic microorganisms g gwere selected from vermicompost (Streptomyces spp) ( p y pp)

②Micro‐ecological adjust agent and soil conditioner plant rhizogenic agentsconditioner plant rhizogenic agents

③ Antibacterial fertilizers and soil regeneration ( Soil‐borne disease of Cucumber seedlings )( Soil‐borne disease of Cucumber seedlings )

④Multi function fertilizers



Bacterium(0103A) from vermicast

College of Resources and Environmental Sciences, CAUmycelium


Bacterium(0103A) from vermicast



E h id dEarthworm peptide and new pesticide‐fertilizernew pesticide‐fertilizer

① 4 peptides extracted from earthworms① 4 peptides extracted from earthworms② effective methods for earthworm peptide

processing （3 patents）③ 3 producing lines in China for the new③ 3 producing lines in China for the new

fertilizer④④ 2 nation certificates for new organic

fertilizer



pesticide-fertilizer made of earthworm in China


pesticide-fertilizer made of earthworm in China


New products and Application of antiNew products and Application of anti‐‐bacterium fertilizers in Chinabacterium fertilizers in Chinabacterium fertilizers in Chinabacterium fertilizers in China

1 Multi1 Multi function biological organic fertilizer:function biological organic fertilizer:1. Multi1. Multi‐‐function biological organic fertilizer:function biological organic fertilizer:Vermicomost and effective microorganismVermicomost and effective microorganism

2 AA f li f ili2 AA f li f ili2. AA foliar fertilizer:2. AA foliar fertilizer:Earthworm and microelementsEarthworm and microelements

3 A ib i l id f ili3 A ib i l id f ili3. Antibacterial peptide fertilizer:3. Antibacterial peptide fertilizer:Biochemistry, earthwormBiochemistry, earthworm

4 V i4 V i4.Vermicompost tea:4.Vermicompost tea:Vermocompost and effective microorganismVermocompost and effective microorganism



Antiblastic effect of vermicompost on some plant disease

发病率（%）

p

d cd

ab

cd dc

a

70

80

鲜重(0.1g)

d d

b b ba

a

40

50

60

70

株高（0 1 )

株高（0.1cm)

d cdc c

b

c c

b b

c c0

10

20

30

发病率（%）

鲜重(0.1g)

株高（0.1cm)

0CK 10%V 20%V 30%V 50%V 20%V0 健康ck 健康20%V

处理

College of Resources and Environmental Sciences, CAU（V-Vermicompost）


prevention and cure effect on cucumber



Application on vegetable production



OOn flowers and ornamentals



Experiment of Earthworm AA additive on fish


p

(Qingdao, Shandong, China)

Cyprus conference, 2016Experiment of Earthworm AA additive on pig

College of Resources and Environmental Sciences, CAUCK AA additive

Cyprus conference, 2016Experiment of vermicompost as culture medium on vegetables (Shunyi, Beijing)



Application of vermicompost on alone

(T h B iji )(Tongzhou, Beijing)


Cyprus conference, 2016Wide foreground of vermiculture and vermiproducts in organic food productiong p



Earthworm (cast) and products processingBio-organic fertilizer

vermicasts Vermicompost tea

D d i iDeodorization

i ti l

Organicwaste

vermicomposting

earthworms

vermiceuticals

Vermimealearthworms Vermimeal

Amino acids


Cyprus conference, 2016earthworm industrial chain

biomedicinebiomedicinefeedstuff

Health Earthworm

fertilizer foods

cosmetics



Medicine enzymes and Cosmetic products



Medicine enzymes from earthworms



College of Resources and Environmental Sciences, CAUCosmetic products made from earthworm

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