72 PATENT ABSTRACTS

4686191

R E C O M B I N A N T P L A S M I D C O N T A I N I N G H U M A N

I N T E R F E R O N - B E T A G E N E

Seiga ltoh, Tatsunari Nishi, Tamio Mizukami, Tadash Matsumoto, Tetsuo Oka, Tadatsagu Taniguchi, Haruo Sugano, Sagamihara, Japan assigned to Kyowa Hakko Kogyo Co Ltd; Juridical Foundation Japanese Foundation for Cancer Researc

Recombinant vector plasmids containing a DNA fragment coding for human interferon-£62 +0 inserted downstream from a tryptophan promoter are useful for transformation of micro- organisms such as Escherichia coil which trans- formants produce human interferon- t 62. + RE

4686319

S Y N T H E S I S O F G E N E T I C F E M A L E S A N D T H E I R U S E I N H Y B R I D S E E D P R O D U C T I O N

Ove Shifriss assigned to Rutgers The State University of New Jersey

A synthetic method is described for the develop- ment of genetic females in squash (Cucurbita pepo L.) through crosses of monoecious varieties or inbreds followed by selection. The genetic females and true-breeding female lines can be propagated sexually following chemical induc- tion of male flowers. Such female lines can be used as seed parents in production of hybrids as well as germplasm for future breeding programs. The new synthetic method is applicable to other monoecious species of plants.

indirect means, a visualization polymer. The visualization polymer is composed of multiple units of a visualization monomer which are covalently linked together directly or indirectly covalently linked together by coupling agentl which bond to chemical groups of the monomer. The monomer may be an enzyme, a tagged poly- peptide, a tagged polyol, a tagged polyolefin or a tagged carbohydrate. The detecting agent may be an antibody, an enzyme, a lectin, strand of a DNA receptor protein, avidin, streptavidin and the like. The visualization polymer produces a high degree of amplification for the detection of the target molecule.

4687733

E A R L Y C A N C E R D E T E C T I O N M E T H O D

Ronald W Trewyn, Ambrosio Steven M D£3 as- signed to Ohio State University

The invention relates to a method of testing for the presence of cancer. An antibody is produced which contains antibodies specific to a modified nucleoside component. The antibody is admixed with a body fluid drawn from a subject mammal. An immunoassay is performed on the admixture to quantify an amount of cancer associated nucleoside present in the fluid and reactive with the antibody.

4687737

M A M M A L I A N S U P P R E S S O R G E N E S

4687732

V I S U A L I Z A T I O N P O L Y M E R S A N D T H E I R A P P L I C A T I O N T O

D I A G N O S T I C M E D I C I N E

David C Ward, Jeffry J Leary, David J Brigati assigned to Yale University

A method for detecting a minute quantity of an inorganic or organic target molecule by com- bining it with a composition of a detecting agent for the target molecule which carries, by direct or

Phillip A Sharp, Mario Capecchi, Uttam RajB- handary, Frank A Laski assigned to Mas- sachusetts Institute of Technology

A method of suppressing a nonsense codon in a gene in a mammalian cell by preparing an oligonucleotide primer having a mismatched anticodon region corresponding to the nonsense codon; preparing a DNA template for produc- tion of a tRNA molecule enabling the insertion of an amino acid when the nonsense codon is translated; forming a suppressor gene from said template and primer by site specific mutagenesis; and transforming the suppressor gene into the cell.