acta scientiae veterinariae 34(supplement 1): 2006

Acta Scientiae Veterinariae 34(Supplement 1): 2006

I

President Speak

Welcome to Araxá!

Minas Gerais is one of the most remarkable States in Brazil. Talk about Minas is talk abouthistory, present in it´s architecture and traditions. This history is particularly noticeable in Araxá,both in the city and in the Hotel hosting the meeting. And Minas Gerais State has always beenlinked to the history of animal reproduction in Brazil. Belo Horizonte city, capital of the State,hosted the 2nd Annual Meeting of the Brazilian Society of Embryo Technology (SBTE), in 1987.Along these almost 20 years, the SBTE has grown, consolidated and reached a dimension noteven imagined that time.

Talk about Minas is talk about animal production. Araxá is located at and region known as“Minas Triangle”, the most important for zebu cattle breeders. And it was the production andtransfer of zebu breed embryos that allowed Brazil to reach the highlighted position and recognitionin embryo transfer scenario. In the last five years, Brazil was responsible for more than 20% ofword embryo activity. But Minas is also important in animal production of other species. TheState is homeland of many equine lineages, hosting herds of great tradition. On the other handdairy goat and ovine production are increasing. In all of these species, embryo transfer has beena valuable tool for genetic improvement and productivity increase.

The scientific program of the meeting was elaborated focusing the main challenges for ETactivity in the different species, including themes as embryo and gamete cryopreservation, embryoproduction in tropical environment and the use of sexed semen in ET and IVF. We would like tothank Professor Ciro Moraes Barros, President of the Scientific Committee and responsible notonly to prepare the main congress program but also to organize the more than 300 papers receivedfor publication. This remarkable participation of the scientific community shows the recognitionreached by the SBTE in this field. We thank to all the members of the scientific committee,which cooperated as reviewers, and in particular to our colleagues Jéferson Fonseca, MarcoAlvarenga and Ana Gomide for translations.

Talk about Minas is talk about hospitality, too. And this is the characteristic we want to theXX Annual Meeting. The organizing committee aims to keep the SBTE tradition of, more thanrepresent only a scientific event, be a moment of congratulation and happiness, where friendsand partners can meet each other and have a good time while sharing professional and lifeexperiences To reach this objective, we expect to offer a little bit of the best of Minas State, in aplace full of meanings. We want to make here our acknowledgements to our colleagues CarlosFernandes and Luiz Sérgio Camargo for their collaboration in the general organization of themeeting.

We thanks to the Brazilian Dairy Cattle Research Center, which was partner of the SBTEin the Meeting, to the support received from the companies which are SBTE regular sponsors,and also to those which came specially to participate in this meeting. We thank to the sponsoragencies CAPES, CNPq and CRMV-MG for recognizing the relevance of the meeting for thescientific progress and professional growth of the participants. Finally, thanks to all colleaguesthat believed and cooperated, directly or indirectly, to the success o four meeting.

João Henrique Moreira VianaPresident - Board 2006-2007


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ORGANIZING COMMITTEE OF THE XIX ANNUAL CONFERENCE OF THE SBTEAugust 24 to 27, 2006

EXECUTIVE BOARD

João Henrique Moreira Viana (President)Alberto Gusmão (Vice-President)

Carlos Antonio Carvalho Fernandes (1o Secretary)Jeferson Ferreira da Fonseca (2o Secretary)

Luiz Sérgio de Almeida Camargo (1o Treasurer)Roberto Sartori Filho (2o Treasurer)

Ciro Moraes Barros (President of the Scientific Commission)

SCIENTIFIC BOARD

EDITORS OF THE PROCEEDINGS:

DESKTOP PUBLISHINGJosé Luis Barbosa de Souza

Ciro Moraes Barros (President of the S.C.)Alberto GusmãoAlceu MezzaliraAlicio Martins Jr.Anneliese de Souza TraldiAntonio de Lisboa Ribeiro FilhoBerenice de Ávila RodriguesCarlos Antonio Carvalho FernandesCezinande de MeiraCláudia Lima Verde LealEd Hoffman MadureiraEduardo Oliveira MeloEunice ObaFernanda da Cruz Landim e AlvarengaFlavio Vieira MeirellesFrederico Ozanan PapaGabriel BóGisele Zoccal MingotiGuilherme de Paula NogueiraIsabel Lima VerdeJeferson Ferreira da FonsecaJoão Carlos Pinheiro FerreiraJoão Henrique Moreira VianaJosé Antonio VisintinJosé Buratini Jr.José Carlos Ferrugem MoraesJosé A. Dell’Aqua Júnior

José Fernando GarciaJosé Luiz Moraes VasconcelosJosé Luiz Rigo RodriguesJosé Nicolau Prospero Puoli FilhoJosé Ricardo de FigueiredoJose Roberto PottiensLuiz Sérgio de Almeida CamargoMara Iolanda B. RubinMarcelo Fábio Gouveia NogueiraMarcelo M. SenedaMarco Antonio AlvarengaMarcos A. L. OliveiraMargot Alves Nunes DodeMaria Denise LopesMario BinelliNereu C. PrestesOsni Lázaro PinheiroOtavio OhashiPaula de Carvalho PapaPaulo Bayard GonçalvesPietro Sampaio BaruselliRafael Herrera AlvarezRicardo Alamino FigueiredoRoberto Sartori FilhoRubens Paes de ArrudaSony Dimas BicudoVera F. M. Hossepian de LimaYeda Watanabe

Ciro Moraes BarrosMarcelo Fábio Gouveia NogueiraRenato Arantes Lima Simões

Thaís NabhanEliana Evaristo de JesusAna Katyucha Gomide


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SBTE IS GRATEFULL FOR THE SUPPORT:

OF THE FOLLOWING GOVERNAMENTAL INSTITUTIONS,

CNPq

CAPES

CRMV-MG

Embrapa Gado de Leite

OF THE SBTE PARTNERS

BIOCOM INDÚSTRIA COMERC E SERVIC LTDAEPPENDORF DO BRASIL LTDA

FELDMAN WILD LEITZ IMPORT. E EXPORT. LTDAFORT DODGE SAUDE ANIMAL LTDA

GENEALOGICA LTDAHERTAPE CALIER DO BRASIL LTDA

INTERVET INTERNATIONAL BVMINITUB DO BRASIL LTDA

NUTRICELL NUTRIENTES CELULARES LTDAPFIZER LTDA

SCHERING-PLOUGHSPECTRUN BIO ENG MED HOSPITALAR LTDA

TECGENE – Produção in vitro de EmbriõesTECNOPEC

TRANSFIX TRANSPLANTES DE EMBRIÕES LTDAVITROGEN


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PROCEEDINGS OF THE XX ANNUAL CONFERENCE OF THEBRAZILIAN EMBRYO TECHNOLOGY SOCIETY (SBTE)

Araxá, MG, Brazil24-27 August 2006

August 24 (Thursday)

08:30 to 12:00 h - Registration

I- PRE-CONFERENCE SHORT COURSES

1. Advances in bovine embryo transfer Chairman: Dr. Ciro Moraes Barros (UNESP, SP) and Dr. Pietro S. Baruselli (USP, SP)

13:00 to 14:00 h - Physiological, pharmacological and endocrine basis of treatments aimingsynchronization and ovulation of follicles in cattle Dr. Mario Binelli (USP,Pirassununga, SP)

14:00 to 15:00 h - Timed Artificial Insemination (TAI) in cows. Dr. José Luís MoraesVasconcelos (UNESP, Botucatu, SP)

15:00 to 16:00 h - Protocols for fixed-time embryo transfer in bovine embryo recipients. Dr.Gabriel A. Bó (Cordoba, Argentina)

16:00 to 16:15 h - Coffee break

16:15 to 18:00 h - Protocols for superstimulation with fixed-time artificial insemination inbovine. Dr. Marcelo F. G. Nogueira (UNESP, Assis, SP; 16:15 to 1700 h) andDr. Pietro S. Baruselli (USP, SP; 17:00 to 18:00 h).

18:00 to 18:15 h - Discussion

2. Advances in equine embryo transfer Chairman: Dr. Marco Antonio Alvarenga (UNESP, Botucatu, SP).

14:00 to 15:00 h - Critical factors on equine embryo transfer programs in Argentina. Dr.Luis Losinno (Argentina)

15:00 to 16:00 h - Critical factors on equine embryo transfer programs in Brazil. Dr. MarcoAntônio Alvarenga (UNESP, Botucatu, SP)

3. Advances in small ruminants embryo transfer Chairman: Dr. Alberto Gusmão (UFBA, BA)

14:00 to 15:00h - Two new treatments to control ovarian activity in goats: short-term protocolfor timed artificial insemination and day 0 protocol for embryo transferprograms. Dr. Edgardo Rubianes (Uruguay)


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15:00 to 16:00h - Early diagnosis of fetal sex by ultrasonography in caprine and ovine species.Dr. Maico Henrique Barbosa dos Santos (UFPE, PE)

16:00 to 16:15h - Coffee break

16:15 to 17:15h - Some aspects of embryo transfer in goats. Dr. Jeferson Ferreira da Fonseca(Embrapa Caprinos, Sobral, CE)

4. Manipulation of preantral follicles Chairman: Dr. José Ricardo de Figueiredo (UECE, CE)

14:00 to 15:00h - State of art of the biotechnique of in vitro manipulation of enclosed inovarian in preantral follicles. Dr. José Ricardo de Figueiredo (UECE, CE)

15:00 to 16:00h - A novel role for angiotensin II in the mechanism of oocyte maturation,follicle development and ovulation in the bovine. Dr. Paulo Bayard DiasGonçalves (UFSM, RS)

19:00 h - Opening Ceremony

II – SBTE ANNUAL MEETING

August 25 (Friday)

08:30 to12:00 h - Registration

Follicular development and oocyte maturationChairman: José Buratini Jr. (UNESP, Botucatu, SP)

08:30 to 09:30 h - Follicular development in mono-ovulatory and poly-ovular species: fromfetus to fertilization. Dr. Robert Webb (UK)

09:30 to 10:00 h - Selected abstracts


10:30 to 11:15 h - Advances on bovine oocyte maturation. Dra. Margot Alves Nunes Dode(Embrapa, Brasília, DF).

11:15 to 12:00 h - Meiosis block and in vitro maturation. Dra. Cláudia Lima Verde Leal (USP,Pirassununga-SP)

12:00 to 14:00 h - Lunch


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Embryo transfer in a tropical environmentChairman: Dr. Ciro Moraes Barros (UNESP, Botucatu, SP)

14:00 to 15:00 h - Embryo transfer as a tool for fertility enhancement of dairy cattle in hotclimates. Dr. Peter J. Hansen (Florida, USA)

15:00 to 15:30 h - Embryo transfer program in dairy cows at tropical environment. Dr. JoséLuiz Moraes Vasconcelos (UNESP, Botucatu, SP)

15:30 to 16:00 h - Importance of sperm genotype (indicus vs taurus) for fertility and embryonicdevelopment at elevated temperatures. Dr. Ciro Moraes Barros (UNESP,Botucatu, SP)

16:00 to 18:00 h - Coffee break and Poster Section I

19:00 h - SBTE Annual Business meeting

20:00 h - Dinner

August 26 (Saturday)

Criopreservation:Chairman: Dr. José Luiz Rigo Rodrigues (UFRGS, Porto Alegre, RG)

08:30 to 09:20 h - Cryopreservation of Mammalian Embryos. Dr. Andraz Dinnyes (Hungary)

09:20 to 10:00 h - Cryopreservation of bovine oocytes and embryos. Dr. Alceu Mezalira(UDESC, SC)


10:30 to 12:00 h - Students Competition

12:00 to 14:00 h - Lunch

14:00 to 16:00 h - Simultaneous Workshops

1. Nuclear transfer and transgenesisChairman: Dr. Flávio V. Meirelles (USP, Pirassununga, SP).

14:00 to 14:45 h - Advances in Cloning and transgenesis in Argentina. Dr. Daniel FSalamone (Argentina).

14:45 to 15:15 h - Challenges for commercial cloning - planning the future. Dr. Flávio V.Meirelles (USP, Pirassununga, SP).


VII

15:15 to 16:00 h - Epigenetics of development: from gametogenesis to embryogenesis. Dra.Flávia L. Lopes (Quebec, Canadá)

2. ICSI and FIV in equineChairman: Dr. Marco Antônio Alvarenga (UNESP, Botucatu, SP)

14:00 to 15:15 h - Intracytoplasmic sperm injection for in vitro equine embryo production.Dr. Luis Losinno (Argentina)

3. Follicular dynamics in sheep and goatChairman: Alberto Gusmão (UFBA, Salvador, BA)

14:00 to 15:00 h - Follicular dynamics, oestrous synchronisation and superovulation insheep. Dr. Edgardo Rubianes (Uruguay)

16:00 to 18:00 h - Coffee break and Poster Section II

20:00 h - Dinner

August 27 (Sunday)

Molecular markersChairman: Luiz Sérgio de Almeida Camargo (EMBRAPA, Juiz de Fora, MG)

08:30 to 09:30 h - Use of molecular markers in embryo transfer programs. Dr. José FernandoGarcia (UNESP, Araçatuba, SP)

09:30 to 10:00 h - Selected abstracts


10:30 to 11:15 h - The use of sexed semen on embryo production. Dr. José Dell’Aqua Júnior(UNESP, Botucatu, SP)

11:15 to 12:00 h - Bovine spermatozoa sexing: when does apply it? Dra. Vera F. M. Hossepiande Lima (UNESP, Jaboticabal, SP)

12:00 to 12:30 h - Closing Ceremony


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CONTENTSActa Scientiae Veterinariae, 34(Supplement 1): 2006

President Letter .................................................................................................................................................... I

Acknolowdgments ............................................................................................................................................... III

Program ................................................................................................................................................................ IV

SHORT COURSE

1. Advances in bovine embryo transfer

PHYSIOLOGICAL, PHARMACOLOGICAL AND ENDOCRINE BASIS OF TREATMENTSAIMING SYNCHRONIZATION AND OVULATION OF FOLLICLES IN CATTLE. Binelli, M;Ibiapina, B.T.; Bisinotto, R.S. ................................................................................................................. 1

TIMED ARTIFICIAL INSEMINATION (TAI) IN COWS Vasconcelos, J.L.M.; Meneghetti, M;Santos, R.M. ............................................................................................................................................ 9

PROTOCOLS FOR FIXED-TIME EMBRYO TRANSFER IN BOVINE EMBRYO RECIPIENTSBó, G.A.; Picinato, D.; Peres, L.; Cutaia, L.; Nasser, L.F.; Baruselli, P.S. ........................................... 17

PRACTICAL ASPECTS AND FUTURE PROSPECTS FOR THE P-36 MODEL OFSUPEROVULATION IN NELORE DONORS. Nogueira, M.F.G.; Barros, C.M. ............................... 25

PROTOCOLS FOR SUPERSTIMULATION WITH FIXED-TIME ARTIFICIALINSEMINATION IN Bos indicus. Baruselli, P.S.; Martins, C.M.; de Sá Filho, M.F.;Gimenes, L.U.; Souza, A.H.; Ayres, H.; Torres-Júnior, J.R.S.; Bó, G.A... ........................................... 31

2. Advances in equine embryo transfer

CRITICAL FACTORS ON EQUINE EMBRYO TRANSFER PROGRAMS IN BRAZIL andARGENTINA. Losinno,L.; Alvarenga, M.A. ....................................................................................... 39

3. Advances in small ruminants embryo transfer

TWO NEW TREATMENTS TO CONTROL OVARIAN ACTIVITY IN GOATS: SHORT-TERMPROTOCOL FOR TIMED ARTIFICIAL INSEMINATION AND DAY 0 PROTOCOL FOREMBRYO TRANSFER PROGRAMS. Menchaca, A.; Rubianes, E. ................................................... 51

EARLY DIAGNOSIS OF FETAL SEX BY ULTRASONOGRAPHY IN CAPRINE AND OVINESPECIES. Santos, M.H.B.; Chiamenti, A.; Moraes, E.P.B.X.; Moura, R.T.D.; Lima, P.F.;Oliveira, M.A.L. .................................................................................................................................... 59

SOME ASPECTS OF EMBRYO TRANSFER IN GOATS. Fonseca, J.F. ........................................... 65

4. Manipulation of preantral follicles

STATE OF ART OF THE BIOTECHNIQUE OF IN VITRO MANIPULATION OF ENCLOSEDIN OVARIAN IN PREANTRAL FOLLICLES Figueiredo, J.R.; Rodrigues, A.P.R.; Santos, R.R.;Lopes C.A.P.; Viana Silva, J.R. ............................................................................................................. 71


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A NOVEL ROLE FOR ANGIOTENSIN II IN THE MECHANISM OF OOCYTE MATURATION,FOLLICLE DEVELOPMENT AND OVULATION IN THE BOVINE Gonçalves, P.B.D.;Ferreira, R.; Portela, V.V.M.; Gasperin, B.G. ........................................................................................ 85

CONFERENCES

FOLLICULAR DEVELOPMENT IN MONO-OVULATORY AND POLY-OVULAR SPECIES:FROM FETUS TO FERTILIZATION. Webb, R.; Dugan, K.; Quinn, R.L.; Fouladi-Nashta, A.A.;Hunter, M.G. .......................................................................................................................................... 95

ADVANCES ON BOVINE OOCYTE MATURATION. Dode, M.A.N.. .......................................... 115

MEIOSIS BLOCK AND IN VITRO MATURATION. Leal, C.L.V.; Adona, P.R. ............................. 131

EMBRYO TRANSFER AS A TOOL FOR FERTILITY ENHANCEMENT OF DAIRY CATTLEIN HOT CLIMATES. Hansen, P.J. ...................................................................................................... 145

EMBRYO TRANSFER PROGRAM IN DAIRY COWS AT TROPICAL ENVIRONMENT.Vasconcelos, J.L.M.; Demétrio, D.G.B.; Santos, R.M.; Jardina, D.T.G.; Rodrigues, C.A.;Chiari, J.R. ........................................................................................................................................... 159

CRYOPRESERVATION OF MAMMALIAN EMBRYOS. Dinnyes, A.; Meng, Q.; Polgar, Z.;Boonkusol, D.; Somfai, T. ................................................................................................................... 171

CRYOPRESERVATION OF BOVINE OOCYTES AND EMBRYOS. Mezzalira, A.;Vieira, A.D. .......................................................................................................................................... 191

USE OF MOLECULAR MARKERS IN EMBRYO TRANSFER PROGRAMS. Garcia, J.F.;Porto-Neto, L.R. .................................................................................................................................. 197

THE USE OF SEXED SEMEN ON EMBRYO PRODUCTION. Dell’Aqua Jr., J.A.; Papa, F.O.;Araújo Jr., J.P.; Freitas, C.P.; Ponchirolli, C.B.; Figueiredo, A.S.; Melo, C.M.; Alberti, K.;Crespilho, A.M.; Siqueira Filho, E.R.; Orlandi, C. ............................................................................. 205

BOVINE SPERMATOZOA SEXING: WHEN DOES APPLY IT? Hossepian de Lima, V.F.M ....... 213

WORKSHOPS

1. Nuclear transfer and transgenesis

AVANCES EN CLONAGEN E TRANSGENESIS NA ARGENTINA. Salamone, D.F. ................... 227

CHALLENGES FOR COMMERCIAL CLONING - PLANNING THE FUTURE Meirelles, F.V.;Providelo, F.D.; Merighe, G.F.K.; Miranda, M.; Traldi, A.; Birgel, E.; Valim, J.R.;Watanabe, Y.F. ..................................................................................................................................... 235

EPIGENETICS OF DEVELOPMENT: FROM GAMETOGENESIS TO EMBRYOGENESIS.Lopes, F.L.; Fortier, A.; La Salle, S.; Lucifero, D.; Trasler, J.M. ....................................................... 243


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2. ICSI and FIV in equine

INTRACYTOPLASMIC SPERM INJECTION FOR IN VITRO EQUINE EMBRYOPRODUCTION. Herrera, C.; Miragaya, H.M.; Losinno, L. .............................................................. 245

3. Follicular dynamics in sheep and goat

FOLLICULAR DYNAMICS, OESTROUS SYNCHRONISATION AND SUPEROVULATIONIN SHEEP Rubianes, E.; Menchaca, A. .............................................................................................. 251

Abstracts Students Competition

PROTOCOL OF EXTRATION OF SPERMATOZOA NUCLEOPROTEIN OF BULL Guardieiro,M.M.; Welter, B.M.; Jacomini, J.O.; Oliveira, F.; Goulart, L.R. ........................................................ 264

EFFECTS OF FIBROBLAST GROWTH FACTOR-2 ON THE IN VITRO CULTURE OFCAPRINE PREANTRAL FOLLICLES Matos, M.H.T.; van den Hurk, R.; Lima-Verde, I.B.; Luque,M.C.A.; Santos, K.D.B.; Martins, F.S.; Báo, S.N.; Lucci, C.M.; Figueiredo, J.R. ........................... 265

INFLUENCE OF DIFFERENT CONCENTRATIONS OF FSH ON CAPRINE PRIMORDIALFOLLICLE DEVELOPMENT Matos, M.H.T.; Lima-Verde, I.B.; Luque, M.C.A.; Maia Jr., J.E.; Silva, J.R.V.; Celestino, J.J.H.; Martins, F.S.; Báo, S.N.; Lucci, C.M.; Figueiredo, J.R. ................. 266

EFFICACY OF TWO TECHNIQUES USING PGE2 TO PROMOTE THE ANTICIPATE

MIGRATION OF EMBRYOS WITH POTENTIAL FOR CRYOPRESERVATION INSUPEROVULATED MARES – PRELIMINARY DATA Peres, K.R.; Arruda, R.P.;Andrade, A.F.C.; Silva, L.C.L.C. ........................................................................................................ 267

CHEMICAL ENUCLEATION AND PROTRUSION FORMATION IN CORTICAL REGIONOF DEMECOLCINE-TREATED BOVINE OOCYTES Saraiva, N.Z.; Perecin, F.; Méo, S.C.;Ferreira, C.R.; Tetzner, T.A.D.; Vantini, R.; Garcia, J.M. ................................................................. 268

PRODUCTION OF CLONED BOVINE EMBRYOS AFTER CHEMICALLY ASSISTEDENUCLEATION BY DEMECOLCINE Saraiva, N.Z.; Perecin, F.; Méo, S.C.; Ferreira, C.R.;Tetzner, T.A.D.; Vantini, R.; Garcia, J.M............................................................................................ 269

OVALBUMIN USED AS PROTEIN SUPPLEMENT ON IN VITRO MATURATION OF BOVINEOOCYTES Tetzner, T.A.D.; Saraiva, N.Z.; Perecin, F.; Ferreira, C.R.; Resende, M.V.;Méo,S.C.; Vantini, R.; Garcia, J.M. ................................................................................................... 270

MAY OVALBUMIN CAUSE DEVIATION OF SEXUAL RATE PROPORTION IN BOVINEEMBRYOS PRODUCED IN VITRO? Tetzner, T.A.D.; Resende, M.V.; Lucio, A.C.; Perini, A.P.;Saraiva, N.Z.; Perecin, F.; Ferreira, C.R.; Méo, S.C.; Vantini, R.; Garcia, J.M. ............................... 271

PREGNANCY RATES OF EMBRYO RECIPIENTS MARES AT FOAL ESTRUS Bueno, L.M.;Freire, A.F.; Freire, S.E.; Andrade, A.F.C.; Arruda, R.P.; Celeghini, E.C.C. ...................................... 272

RELATIVE QUANTIFICATION OF APOPTOSIS GENES IN BOVINE PLACENTA FROMNUCLEAR TRANSFERED PREGNANCYS Braga, F.C.; Miglino, M.A.; Ripamonte, P.;Merighe, G.K.F.; Meirelles, F.V. ......................................................................................................... 273


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FOLLICULAR ASPIRATION IN THE PERI-LUTEOLYTIC PERIOD IN CATTLE: EFFECTSON FOLLICULAR GROWTH AND ESTROUS CYCLE LENGTH Bisinotto, R.S.; Ibiapina,B.T.; Pontes, E.O.; Fedozzi, F.; Bergamaschi, M.A.C.M.; Bertan, C.M.; Binelli, M. ....................... 274

DIMETHYL FORMAMIDE IN RAM SEMEN CRYOPRESERVATION Fidelis, A.A.G.;Polidoro, R.F.Q.; Blume, H.; Mondadori, R.G. ................................................................................... 275

STRATEGIES INVOLVING OOPLAST TRANSFER TO CONTROL CYTOPLASMICGENETIC INHERITANCE IN BOVINE EMBRYOS Ferreira, C.R.; Meirelles, F.V.; Chiaratti,M.R.; Perecin, F.; Méo, S.C.; Burgstaller, J.; Steinborn, R.; Tetzner, T.A.D.; Saraiva, N.Z.;Landim Jr., L.P.; Yamazaki, W.; Vantini, R.; Garcia, J.M. .................................................................. 276

BOVINE SPERMATOZOON AS VECTOR FOR TRANSGENESIS: INTERACTION OFEXOGENOUS DNA AND SPERM VIABILITY Feitosa, W.B.; Mendes, C.M.; Milazzotto, M.P.;Martins, E.A.L.; Rocha, A.M.; Avanzo, J.L.; Visintin, J.A.; Assumpção, M.E.O.A. ......................... 277

OVARIAN SUPEROVULATORY RESPONSE FOLLOWING SYNCHRONIZATION OFFOLLICULAR WAVE EMERGENCE WITH FOLLICLE ABLATION IN MARES Orlandi, C.;Bergfelt, D.R.; Wechsler, F.S.; Nogueira, G.P.; Puoli Filho, J.N.P.; Meira, C. ................................... 278

CULTURE OF CLONED BOVINE EMBRYOS PRODUCED WITH DIFFERENT CELLDONORS USING THE WOW MODIFIED SYSTEM Feltrin, C.; Forell, F.; Arruda, N.S.;Silva, D.S.; Queiroz, L.M.V.; Peixer, M.A.S.; Malard, P.F.; Santana, G.M.; Rodrigues, J.L. ............ 279

MICROINJECTED EMBRYO TRANSFER WITH HUMAN G-CSF GENE TO PRODUCETRANSGENIC GOATS IN BRAZIL Avelar, S.R.G.; Moura, R.R.; Silva, J.D.A.; Pereira, A.F.;Almeida, A.P.; Andrade, M.L.L.; Cajazeiras, J.B.; Teixeira, D.I.A.; Lopes Júnior, E.S.; Dias, L.P.B.;Andreeva, L.E.; Serova, I.A.; Serov, O.; Freitas, V.J.F. ...................................................................... 280

LUTEAL ECHOGENICITY EVALUATION USING DIFFERENT ULTRASOUND PROBESSiqueira, L.G.B.; Viana, J.H.M.; Diniz, E.S.; Camargo, L.S.; Amorim, L.S.; Fonseca, J.F.;Fernandes, C.A.C.; Torres, C.A.A. ..................................................................................................... 281

OESTROUS AND OF THE OVULATION SYNCHRONIZATION, ARTIFICIALINSEMINATION IN FIXED TIME AND PREGNANCY VALUES IN NELORE MILK COWSNascimento, V.A.; Torres, C.A.A.; de Oliveira, M.M.N.F.; Logrado, M.L.; Viana, J.H.M.;da Costa, E.P.; Sousa, K.R.S.; Carneiro, C.; Oliveira, F.A. ................................................................ 282

EFFICIENCY OF OVULATION SYNCHRONIZATION PROTOCOLS ON NELORE COWSIN DIFFERENT GEOGRAPHICAL AREAS Nascimento, V.A.; Torres, C.A.A.;de Oliveira, M.M.N.F.; de Oliveira, R.F.M.; Mourão, L.L.; da Silva, A.F. ........................................ 283

EFFECT OF MATERNAL HEAT- STRESS ON OOCYTE QUALITY AND IN VITROCOMPETENCE IN Bos indicus CATTLE Torres-Júnior, J.R.S.; Pires, M.F.Á.; Sá, W.F.; Ferreira,A.M.; Viana, J.H.M.; Camargo, L.S.A.; Ramos, A.A.; Folhadella, I.M.; Polisseni, J.; Freitas, C.;Clemente, C.A.A.; Sá Filho, M.F.; Souza, A.H.; Martins, C.M.; Baruselli, P.S. .............................. 284

HEIFERS CALVING DATE IN THE BODY CONDITION SCORE AND IN THE RESPONSETO A TIMED ARTIFICIAL INSEMINATION PROTOCOL Meneghetti, M.; Losi, T.C.;Vilela, E.R.; Martins Jr., A.P.; Vilela Neto, J.M.; Vasconcelos, J.L.M. ............................................. 285


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EFFECT OF PROGESTERONE AND/OR 17β-OESTRADIOL TREATMENT BEFOREINDUCTION OF OVULATION ON SUBSEQUENT LUTEAL LIFESPAN IN ANOESTROUSNELORE COWS Sá Filho, O.G.; Dias, C.C.; Vasconcelos, J.L.M. ................................................... 286

Abstracts - Best Work Applied Area

SUPEROVULATORY RESPONSE OF BOVINE DONORS USING FIVE OR EIGHTINJECTIONS OF SWINE FSHp Garcia, P.D.; Lemos, F.O.; Moreira, E.M.; Lemos, T.N.;Hucke, E.E.T.S. ................................................................................................................................... 289

INFUENCE OF HIGH OR LOW FEED INTAKE ON IN VITRO EMBRYO PRODUCTION INCATTLE Martins, A.C.; Ramos, A.F.; Mollo, M.R.; Pivato, I.; Camara, J.U.; Carrijo, L.H.D.;Driessen, K.; Rumpf, R.; Sartori, R. ................................................................................................... 290

MORPHOMETRY OF OVARIAN STRUCTURES IN EMBRYO DONOR SAANEN GOATSUSING REAL-TIME ULTRASONOGRAPHY Sousa, F.C.; Pinheiro, E.S.P.; Rondina, D.;Freitas, V.J.F.; Teixeira, D.I.A. ............................................................................................................ 291

DETECTION OF OVULATION FAILURE PERMITS EARLY RESYNCHRONIZATION ANDINCREASES THE NUMBER OF PREGNANT COWS IN 28 DAYS Vasconcelos, J.L.M.;Garcia, P.H.M. ..................................................................................................................................... 292

FACTORS THAT AFFECT CONCEPTION RATE FOLLOWING ARTIFICIAL INSEMINATIONOR EMBRYO TRANSFER IN LACTATING HOLSTEIN COWS Demétrio, D.G.B.;Rodrigues, C.A.; Santos, R.M.; Demétrio, C.G.B.; Chiari, J.R.; Vasconcelos, J.L.M. ...................... 293

Abstracts - Best Work Basic Area

BUCK (Capra hircus) GENES ENCODE NEW MEMBERS OF THE SPERMADHESIN FAMILYMelo, L.M.; Teixeira, D.I.A.; Havt, A.; Cunha, R.M.S.; Rádis-Baptista, G.; Freitas, V.J.F.;Cavada, B.S. ........................................................................................................................................ 297

DEMECOLCINE EFFECTS ON NUCLEAR AND CITOPLASMATIC MATURATION OFBOVINE OOCYTES Saraiva, N.Z.; Bueno da Silva, R.; Oliveira Júnior, E.G.; Perecin, F.;Vantini, R.; Garcia, J.M....................................................................................................................... 298

CONTROL OF EARLY FOLLICULAR DEVELOPMENT IN GOATS BY ACTIVIN-A ANDFOLLISTATIN Silva, J.R.V.; Santo, R.R.; Rodrigues, A.P.R.; Figueiredo, J.R.;van den Hurk, R. .................................................................................................................................. 299

POLY(A) mRNA CONTENT VARIATION IN SINGLE BOVINE OOCYTES AND PRE-IMPLANTATION EMBRYOS Biase, F.H.; Merighe, G.K.F.; Biase, W.K.F.S.;Martelli, L.; Meirelles, F.V. ................................................................................................................. 300

FOLLICULAR ASPIRATION ON PERI-LUTEOLYSIS PERIOD IN BOVINE: EFFECTS ONCHARACTERISTICS OF THE LUTEAL PHASE Ibiapina, B.T.; Bisinotto, R.S.; Pontes, E.O.;Fedozzi, F.; Bertan, C.M.; Bergamaschi, M.A.C.M.; Binelli, M. ....................................................... 301

THE ROLE OF LEPTIN DURING MATURATION OF BOVINE OOCYTES Paula-Lopes, F.F.;Boelhauve, M.; Habermann, F.; Sinowatz, F.; Wolf, E. ...................................................................... 302

BOVINE RNA EXPRESSION OF OOCYTE AND EMBRYO SPECIFIC GENES Biase, F.H.;Biase, W.K.F.S.; Merighe, G.K.F.; Puga, R.; Giuliatti, S.; Martelli, L.; Meirelles, F.V. .................... 303


XIII

EXPOSURE TO EXOGENOUS PROGESTERONE FOR 3 DAYS BEFORE INDUCTION OFOVULATION AVOIDS PREMATURE LUTEOLYSIS IN ANESTROUS NELORE COWSSá Filho, O.G.; Zilioti, C.R.; Vasconcelos, J.L.M. .............................................................................. 304

Abstracts - Cryopreservation

EFFECT OF VITAMIN E IN BORORO’S DEER FROZEN SEMEN (Mazama nana) Abreu,C.O.; Martinez, A.C.; Moraes, W.; Carvalho, A.; Juvenal, J.C.; Corteze, A.A. ................................. 307

EFFECT OF DIFFERENT CONCENTRATIONS OF SEMINAL PLASMA ON PROTECTIONAGAINST OXIDATIVE STRESS AND CELL VIABILITY ON CRYOPRESERVATION OFEQUINE SPERMATOZOA Almeida, J.L.; Andrade, A. Jr.; Zimmermann, M.F.;Dode, M.A N.; Neves, J.P. ............................................................................................................... 308

REDUCTION IN CRYOPROTECTANT CONCENTRATIONS ON THE VITRIFICATION OFIMMATURE BOVINE OOCYTES, UNDER A HIGH COOLING RATE. Bunn, S.; Bertolini,M.; Cruz, F.B.; Martins, L.T.; Gaudêncio Neto, S.; Wentz, K.C.; Paulini, F.; Munhoz, J.J.;Mezzalira, A. ....................................................................................................................................... 309

COMPARISON OF TWO EXTENDERS FOR LOW-DOSE BULL SEMENCRIOPRESERVATION Crespilho, A.M.; Papa, F.O.; Alberti, K.; Siqueira Filho, E.R.;Martins Jr., A.; Novaes, J.L.C. ............................................................................................................ 310

IN VITRO VIABILITY OF BULL SEMEN CRYOPRESERVED WITH TWO EXTENDERSCONTAINING REDUCED SPERM NUMBERS Martins Jr., A.; Calegari, R.S.; Crespilho, A.M.;Papa, F.O. ............................................................................................................................................ 311

MOUSE SPERM CELL FREEZING USING SUCROSE AND SKIM MILK Lucca Neto, J.A.;Amaral, V.L.L.; Cordini, M.; Frajblat, M. .......................................................................................... 312

COMPARISSON OF THE EFFICIENCY OF TWO VITRIFICATION SOLUTIONS FOR MOUSEMORULAE Amaral, V.L.L.; Cordini, M.; Salvador, R.A.; Weingril, R.B.; Mezzalira, A.;Frajblat M. ........................................................................................................................................... 313

INFLUENCE OF EMBRYONIC DIAMETER AND EFFECT OF SUCROSE ADDITION TOTHE VITRIFICATION MEDIUM ON SURVIVAL RATES OF IN VITRO PRODUCED BOVINEEMBRYOS Varago, F.C.; Vasconcelos, A.B.; Oliveira, C.H.; Alvim, M.T.T.; Saliba, W.P.;Elias, N.Q.; Lagares, M.A. ................................................................................................................. 314

EFFICIENCY ANALYSIS OF A PORTABLE COOLER ON CRYOPRESERVATION OFBOVINE SEXED SEMEN Franco, R.V.R.; Oliveira, K.M.; Reis, F.A.C.; Nishikawa, M.F.A.;Braga, M.Q.; Rodrigues, L.F.; Galeli, G.; Moura, E.P.; Tavares, M. .................................................. 315

DIFFERENTS COOLING RATES FOR GOAT SEMEN CRYOPRESERVATION Rovay, H.;Torres, C.A.A.; Furst, R.; Palhão, M.P.; Araújo, R.R.; Bispo, C.A.S.; Rodrigues, A.L.;Zambrini, F.N. ..................................................................................................................................... 316

ULTRASTRUCTURAL ANALYSIS OF IMMATURE EQUINE OOCYTES AFTERVITRIFICATION WITH PVA COPOLYMER Curcio, B.R.; Boff, A.L.N.; Lins, L.; Leite, D.S.P.;Barros, S.S.; Nogueira, C.E.W.; Deschamps, J.C. .............................................................................. 317


XIV

COMPARISON BETWEEN TWO EQUILIBRIUM PERIODS FOR THE FREEZINGDOMESTIC CAT (Felis catus) SEMEN Villaverde, A.I.S.B.; Melo, C.M.; Taconeli, C.A.;Papa, F.O.; Lopes, M.D. ...................................................................................................................... 318

THE EFFECT OF STABILIZATION PERIOD ON THE QUALITY OF CRYOPRESERVEDGOAT SEMEN Bittencourt, R.F.; Ribeiro Filho, A. de L.; Chalhoub, M.; Alves, S.G.G.;Biscarde, C.E.A.; Vasconcelos, M.F.; Silva, A.A.B.; OBA, E. ........................................................... 319

TWO COOLING RATES AND TWO EQUILIBRATION PERIODS FOR CRYOPRESERVATIONOF GOAT SEMEN Bittencourt, R.F.; Ribeiro Filho, A. de L.; Alves, S.G.G.; Vasconcelos, M.F.;Biscarde, C.E.A.; Santana, R.C.M.; Brandão, A.C.; OBA, E. ............................................................ 320

EFFECTS OF EDTA, EQUEX STM PASTE AND THE SOYBEAN LECITHIN ON THE POST-THAWING GOAT SPERMATOZOA VIABILITY Bittencourt, R.F.; Ribeiro Filho, A. de L.;Vasconcelos, M.F.; Alves, S.G.G.; Biscarde, C.E.A.; Brandão, A.C.; OBA, E. .................................. 321

VITRIFICATION OF IMMATURE BOVINE OOCYTES LOADED IN CONTAINERS WITHDISTINCT HEAT CONDUCTIVITIES Bunn, S.; Bertolini, M.; Cruz, F.B.; Vieira, A.D.;Pedrazzi, C.; Cesaro, M.P.; Ortigari, I.; Ribeiro, E.S.; Mezzalira, J.C.; Mezzalira, A ....................... 322

EFFECT OF THE REMOVAL OF THE SEMINAL PLASMA ON THE FERTILITY OF FROZENBOVINE SEMEN Alberti, K.; Papa, F.O.; Siqueira Filho, E.R.; Crespilho, A.M.; Martins Jr., A.;Dellaqua Jr., J.A. ................................................................................................................................. 323

EFFECT OF TWO DIFFERENT DILUENTS AND TWO DIFFERENT CHILLED SYSTEMSON DOGS SEMEN VIABILITY Ferreira, M.A.R.; Barcellos, B.P.; Brandão, F.Z.;Nogueira, L.A.G.; Santos L.J.; Pinho, T.G. ......................................................................................... 324

Abstracts - Physiology / Endocrinology of Female

HISTOPATHOLOGY RESULTS FOLLOWING SERIAL ENDOMETRIAL SAMPLE BIOPSIESIN NELORE (Bos taurus indicus) COWS THROUGHOUT THE ESTROUS CYCLEMaziero, R.R.D.; Martin, I.; Mattos, M.C.C.; Teixeira, L.B.C.; Oba, E.; Ferreira, J.C.P. ................. 327

IMMUNOHISTOCHEMICAL DETECTION OF PROGESTERONE AND ESTROGENRECEPTORS IN THE ENDOMETRIUM OF NELORE COWS (Bos taurus indicus) DURINGTHE ESTROUS CYCLE Martin, I.; Rodrigues, M.M.P.; Torres Neto, R.; Weschler, F.S.;Láufer-Amorim, R.; Oba, E.; Ferreira, J.C.P. ..................................................................................... 328

OXYTOCIN RECEPTOR GENE EXPRESSION IN THE ENDOMETRIUM OF NELORE COWS(Bos taurus indicus) DURING THE ESTROUS CYCLE Martin, I.; Costa, I.B.; Machado, M.F.; Castilho, A.C.S.; Vetoratto, L.F.; Buratini Jr., J.; Oba, E.; Ferreira, J.C.P. ....................................... 329

EFFECT OF CLONIDINE (α2-ADRENERGIC RECEPTOR AGONIST) TREATMENT ON LHCONCENTRATION IN PRE-PUBERTAL NELLORE HEIFERS Oliveira, D.J.C.;Nogueira, G.P. ...................................................................................................................................... 330

EFFECT OF DIFFERENT SUPPLEMENTATION LEVELS ON THE REPRODUCTIVEACTING OF DAIRY COWS MAINTAINED ON PASTURE Salmazo, R.; Santos, G.M.G.;Mizubuti, I.Y.; Moreira, F.B.; Ribeiro, E.L.A.; Rocha, M.A.; Costa, M.C.; Silva, K.C.F.;Schroeder, R.V.; Rigo, A.G.; Seneda, M.M. ........................................................................................ 331


XV

EXPRESSION OF PROGESTERONE RECEPTOR IN CANINE PLANCENTA DURINGPREGNANCY AND PARTURITION Veiga, G.A.L.; Milazzotto, M.P.; Gonçalves, J.S.A.; Lúcio, C.F.; Silva, L.C.G., Visintin, J.A.; Vannucchi, C.I. ................................................................. 332

EFFECT OF TRIAMCINOLONE PRETREATMENT PRIOR TO INDUCTION OFPARTURITION ON THE INTERVAL FROM INDUCTION TO CALVING IN BOVINERECIPIENTS Rezende; L.F.C.; Zúccari, C.E.S.N.; Ayres, H.; Torres-Júnior, J.R.;Rezende, C.R.L.; Nasser, L.F. ............................................................................................................. 333

EFFECT OF TRIAMCINOLONE PRETREATMENT PRIOR TO INDUCTION OFPARTURITION ON THE INCIDENCE OF RETAINED PLACENTA IN BOVINE RECIPIENTSRezende; L.F.C.; Zúccari, C.E.S.N.; Ayres, H.; Torres-Júnior, J.R.; Rezende, C.R.L,Nasser, L.F. .......................................................................................................................................... 334

DEVELOPMENT OF A SURGICAL TECHNIQUE FOR MONITORING THE UTERINEMICROENVIRONMENT IN CATTLE. Lima, M.C.; Freire, A.F.; Fedozzi, F.; Pimentel, J.R.V.;Hucke, E.E.T.S.; Arruda, R.P.; Assumpção, M.E.O.A.; Miglino, M.A.; Binelli, M. ......................... 335

EFFECTS OF A METHOD OF UTERINE MICRO-WASHINGS ON THE LUTEAL FUNCTION,FOLLICULAR GROWTH AND MAINTENANCE OF EARLY PREGNANCY IN CATTLEFreire, A.F.; Lima, M.C.; Fedozzi, F.; Pimentel, J.R.V.; Hucke, E.E.T.S.; Arruda, R.P.;Assumpção, M.E.O.A.; Miglino, M.A.; Binelli, M. ........................................................................... 336

VALIDATION OF A BIOLOGICAL ASSAY TO QUANTIFY THE COMPETENCE OF BOVINECONCEPTUSES Marques, V.B.; Bertan, C.M.; Pontes, E.O.; Miguez, P.H.P.; da Cunha, P.M.;Lima, M.C.; Bressan, F.F.; Miranda, M.S.; Meirelles, F.V.; Papa, P.C.; Miglino, M.A.;Binelli, M. ........................................................................................................................................... 337

INFLUENCE OF AGE ON DEGENERATION RATES OF PREANTRAL OVARIANFOLLICLES IN BITCHES Alves, A.E.; Perini, A.P.; Covizzi, G.J.; Apparício, M.F.;Ribeiro, A.P.C.; Pires, E. A.; Vicente, W.R.R. .................................................................................... 338

ASSESSMENT OF EPIDERMAL MATURITY AT THE DELIVERY MOMENT OF THENELORE CALVES OBTAINED BY ARTIFICIAL INSEMINATION AND EMBRYOS IN VIVOAND IN VITRO PRODUCTION Moya, C.F.; Prestes, N.C.; Piagentini, M.; Rocha, N.S. ............... 339

STANDARDIZATION OF GENE EXPRESSION ANALYSIS METHOD IN CANINE OOCYTEAND CUMULUS CELLS Gonçalves, J.S.A.; Milazzotto, M.P.; Marques, M.G.;Nascimento, A.B.; Martins, L.F.; Vannucchi, C.I.; Assumpção M.E.OA; Visintin, J.A. ................... 340

RATES OF OOCYTES RECOVERY IN BITCHES Gonçalves, J.S.A; Milazzotto, M.P.; Marques,M.G.; Simões, R.; Feitosa, W.B.; Goissis, M.D.; Nascimento, A.B.; Vannucchi, C.I.;Assumpção, M.E.O.A.; Visintin, J.A. ................................................................................................. 341

EFFECT OF ACUTE STRESS ON ESTROUS BEHAVIOR AND OVULATION IN COWSDURING THE PERIOVULATORY PHASE Maziero, R.R.D.; Martins, A.C.; Mollo, M.R.; Bastos,M.R.; Ferreira, J.G.S.; Siqueira Filho, E., Ramos, A.F.; Driessen, K.; Comegno Jr, L.C.;Leme, L.O. 2,3; Rumpf, R2; Sartori, R. .............................................................................................. 342

ENHANCEMENT OF LUTEAL FUNCTION IN NELLORE COWS (Bos taurus indicus)SUPLEMENTED WITH GNRH AND HCG Bergamaschi, M.A.C.M. ; Machado, R.;Figueiredo, R.A.; Barbosa, R.T.; de Oliveira, C.A.; Giassetti, M.I.; Binelli, M. ............................... 343


XVI

MECHANICAL OR PHARMACOLOGICAL REMOVAL OF THE DOMINANT FOLLICLEAS AN ANTI-LUTEOLYTIC STRATEGY IN CATTLE Machado, R.; da Silva, J.C.B.;Barbosa, R.T.; Bisinotto, R.S.; Siqueira, A.F.P.; Binelli, M. .............................................................. 344

INSULIN EFFECTS ON THE EWE´S SUPEROVULATION Brandão, H.M.; Silva, M.N.;Vinholis, M.M.B.; Ferrão, A.A.; Traldi, A.S. ..................................................................................... 345

PROGESTERONE CONCENTRATIONS IN SUPEROVULATED HOLSTEIN HEIFERSSUPPLEMENTED WITH TWO DOSAGES OF BETACAROTENE ASSOCIATED WITHTOCOPHEROL Sales, J.N.S.; Souza, J.C. ......................................................................................... 346

EFFECT OF A SHORT PERIOD OF LIGHT EXPOSURE FOR THE ADVANCE OF OVARIANACTIVITY OF MARES IN SEASONAL ANESTRUS Devito, L.G.; Carmo, M.T.;Alvarenga, M.A.; Santos, J.F.; Oliveira, J.V. ...................................................................................... 347

EVALUATION OF THE USE OF FLUNIXIN MEGLUMINE ON THE SERIC LEVELS OFPROGESTORE IN HYBRID HEIFERS AND COWS Pinto-Neto, A.; Lucca, F.M.; Alberton, J.;Mota, M.F.; Borges, A.M.; Acco, A.; Fonseca, J.F.; Silva, A.V. ......................................................... 348

GESTODENE A NEW PROGESTAGEN WITH HIGH ACTIVITY FOR CONTROL OF THECYCLE OESTROUS IN BOVINE FEMALE Granito, A.L.; Pimentel, J.R.V.; Andrade, A.F.C.;Pardo, F.D.; Almeida, A.B.; Braga, F.A.; Madureira, E.H. ................................................................. 349

EFFECT OF PROSTAGLANDIN F2α INJECTION 3 DAYS AFTER OVULATION ON THEESTRUS CYCLE OF MARES-PARTIAL RESULTS Oliveira, J.V.; Alvarenga, M.A.;Carmo, M.T.; Araújo, G.H.M.; Santos, J.F. ......................................................................................... 350

ESTROUS RESPONSE OF SHEEP WITH SHORT- OR LONG-TERM SYNCHRONYPROTOCOLS AND GNRH - PRELIMINARY RESULTS Almeida, A.K.; Chalhoub, M.; RibeiroFilho, A. de L.; Freitas, D. S.; Alves, S.G.G.; Bittencourt, R.F.; Santana, R.C.M.;Brandão, A.C.G.; Gusmão, A.L. .......................................................................................................... 351

IN VITRO CULTURE OF CAPRINE PREANTRAL FOLLICLES IN DIFFERENTCONCENTRATION AND SOURCE OF SERUM Bruno, J.B.; Martins, F.S.; Lima-Verde, I.B.;Matos, M.H.T.; Wanderley, L.S.; Correia, J.C.; Silva, J.R.V.; Figueiredo, J.R.;Rodrigues, A.P.R. ................................................................................................................................ 352

EVALUATION OF THE ABORTIVE EFFICIENCY, INTERVAL OF THE ABORTION ANDRETENTION OF PLACENTA AFTER THE APPLICATION OF D CLOPROSTENOL SODICIN NELORE COWS Cavalca, L.G.; Verneck, W.C.; Detoni, A.L.; Marques, L.F.A.; Nunes, L.C. ... 353

EVALUATION OF THE FERTILITY AFTER INDUCED ABORTION WITH DCLOPROSTENOL SÓDIC IN NELORE COWS Cavalca, L.G.; Verneck, W.C.; Detoni, A.L.;Marques, L.F.A.; Nunes, L.C. ............................................................................................................. 354

PRODUCTION OF 13,14-DIIDRO 15-CETO F2α PROSTAGLANDIN (PGFM) IN RESPONSETO OCITOCIN IN NELORE COWS DURING EARLY POSTPARTUM Satrapa, R.A.;Pinheiro, V.G.; Ereno, R.L.; Bertan, C.M.; Piagentini, M.; Binelli, M.; Barros, C.M. ...................... 355

RELATIONSHIP BETWEEN OVULATORY FOLLICLE SIZE, CORPUS LUTEUM AREA ANDCONCEPTION RATE IN BEEF CATTLE USING FIXED-TIMED ARTIFICIALINSEMINATION Gênova, L.G.; Delboni, M.I.; Seneda, M.M. ......................................................... 356


XVII

USE OF DIFFERENT eCG DOSES IN PROTOCOLS OF ESTRUS INDUCTION ANDSYNCHRONIZATION FOR LACTATING DAIRY GOATS (PRELIMINARY RESULTS) Palhão,M.P.; Bispo, C.A.S.; Rovay, H.; Carvalho, G.R.; Rodrigues, M.T.; Fonseca, J.F.,Zambrini, F.N., Rodrigues, A.L. .......................................................................................................... 357

NITRIC OXIDE MODULATES 17β - OESTRDIOL BUT NOT PROGESTERONE SYNTHESISVIA GMPC IN ANTRAL GRANULOSA CELLS CULTERED IN SERUM-FREE MEDIUMFaes, M.R.; Caldas-Bussiere, M.C.; Viana, K.S.; Costa, F.R.; Escocard, R.M. ................................ 358

GOAT ESTRUS INDUCTION AND SYNCHRONIZATION PROTOCOLS DURINGANESTROUS SEASON Zambrini, F.N.; Costa, E.P.; Fonseca, J.F.; Bruschi, J.H.; Viana, J.H.M.;Soria, G.F.; Amorim, L.S.; Palhão, M.P.; Carvalho, B.P.; Rovay, H. .................................................. 359

LH SERUM CONCENTRATION IN CYCLING OR OVARIECTOMIZED NELORE COWSSUBJECTED TO TREATMENT WITH 2.0 MG OF ESTRADIOL BENZOATE Maio, J.R.G.; Sandoval, G.A.F.; Souza, E.D.F.; Nogueira, G.P.; Cipriano, R.S.; Perecin, F.;Garcia, J.M. ......................................................................................................................................... 360

ARE PROGESTINS ASSOCIATED TO ESTRADIOL CAPABLE TO INDUCE FOLLICULARATRESIA IN GONADOTROPHINS LOW DEPENDENT FOLLICLES? Siqueira, L.C.;Ferreira, R.; Loguercio, R.S.; Borges, L.F.K.; Oliveira, J.F.C.; Barreto, K.P.; Gonçalves, P.B.D. .... 361

EXPRESSION OF FIBROBLAST GROWTH FACTORS 7 AND 10 (FGF-7 AND FGF-10) INBOVINE PREANTRAL FOLLICLES Pinto, M.G.L.; Castilho, A.C.S.; Amorim, R.L.;Price, C.A.; Buratini Jr., J. .................................................................................................................. 362

REGULATION OF FIBROBLASTIC GROWTH FACTOR RECEPTOR 2b (FGFR-2b) GENEEXPRESSION DURING BOVINE LUTEAL DEVELOPMENT Castilho, A.C.S.; Giometti,I.C.; Costa, I.B.; Machado, M.F.; Teixeira, N.A.; Papa, P.C.; Price, C.A.;Buratini Jr., J. ...................................................................................................................................... 363

IDENTIFICATION AND QUANTIFICATION OF MOLECULES ASSOCIATES TO THEGLUCOSE METABOLISM IN ZEBU COWS’ OVIDUCT Gomes , H.F.; Dias, A.J.B.;Logullo, C.; Paes de Carvalho, C.S.; de Souza Filho, G.A.; Ferreira, B.S. ........................................ 364

OOCYTE MATURATION AND PROGESTERONE PRODUCTION IN BITCHES Apparício,M.; Vicente, W.R.R.; Ribeiro, A.P.C.; Pires, E.A.; Alves, A.E.; Covizzi, G.J.;Nogueira, G.P.; Gadelha, C.R.F. .......................................................................................................... 365

LH AND FSH PROFILES ON FOLLICULAR DEVIATION IN BUFFALO (Bubalus bubalis)HEIFERS Gimenes, L.U.; Sá Filho, M.F.; Carvalho, N.A.T.; Torres-Júnior, J.R.S.; Souza, A.H.;Vannucci, F.S.; Bianconi, L.L.; Bisinotto, R.S.; Reichert, R.; Beltran, M.P.; Nogueira, G.P.;Baruselli, P.S. ...................................................................................................................................... 366

LH AND FSH PROFILES ON FOLLICULAR DEVIATION IN NELORE (Bos indicus) HEIFERSGimenes, L.U.; Sá Filho, M.F.; Torres-Júnior, J.R.S.; Souza, A.H.; Beltran, M.P.; Nogueira, G.P.;Binelli, M.; Baruselli, P.S. ................................................................................................................... 367

FACTORS THAT AFFECT THE CORPUS LUTEUM VOLUME DURING ESTROUS CYCLEIN HIGH PRODUCER HOLSTEIN COWS Souza, A.H.; Wosniacki, A.M.; Torres-Júnior, J.R.S.;Martins, C.M.; Ayres, H.; Baruselli, P.S. ............................................................................................ 368


XVIII

EFFECTS OF MULTIPLE TREATMENTS WITH ESTRADIOL CYPIONATE (ECP) IN THEEARLY POSTPARTUM ON THE MILK PRODUCTION IN HIGH PRODUCING HOLSTEINCOWS Souza, A.H.; Viechnieski, S.; Rodrigues, A.C.O.; Ayres, H.; Baruselli, P.S. ......................... 369

BIOPHYSICAL CHARACTERISTICS OF UTERINE MILIEU INTERIEUR IN NELORE COWSSUBMITTED TO OVULATION AND ESTROUS SYNCHRONIZATION PROTOCOLSNascimento, V.A.; Torres, C.A.A.; de Oliveira, M.M.N.F.; Mourão, L.L.; Carneiro, C.; Dias, M.;Sousa, K.R.S.; Oliveira, F.A.; Viana, J.H.M. ...................................................................................... 370

EFFICIENCY OF TWO DOSES OF SODIC CLOPROSTENOL FOR LUTEOLYSIS IN BOVINEAT DIFFERENT DAYS OF ESTROUS CYCLE Fernandes, C.A.C.; Vasconcelos, T.D.;Oliveira, E.R.; Alves, B.F.L.; Figueiredo, A.C.S.; Gioso, M.M.; Oba, E. .......................................... 371

LEPTIN EFFECTS ON NELORE HEIFERS (Bos taurus indicus) PUBERTY – PRELIMINARYREPORT Beltran, M.P.; Nogueira, G.P.; Delavaud, C.; Chilliard, Y. ................................................. 372

Abstracts - Physiology / Endocrinology of Male

SERUM CONCENTRATION OF TESTOSTERONE AND ITS RELATIONSHIP WITH THESCROTAL CIRCUMFERENCE AND THE SPERMATIC QUALITY OF NELORE BREEDBULLS Welter, B.M.; Jacomini, J.O.; Guardieiro, M.M.; Meira, C.; Diniz, E.G.;Guimarães, E.C.; Reis, F.C. ................................................................................................................. 375

CORRELATION BETWEEN DNA INTEGRITY AND HIGH LEVELS OF PROXIMALCYTOPLASMATIC DROPLETS IN BOVINE SPERMATOZOA Koivisto, M.B.; Carreira, J.T.;Mingoti, G.Z.; Perri, S.H.V.; Rodrigues, L.H.; Rödel, E.U.R. ............................................................ 376

SEMINAL CHARACTERISTICS FROM NELORE BREED BULLS CONFINED ANDTREATED WITH RECOMBINANT BOVINE SOMATOTROFIN (R-BST) Amorim, L.S.; Torres,C.A.A.; Amorim, E.A.M.; Guimarães, J.D.; Silva Filho, J.M.; Fonseca, J.F.; Viana, J.H.M.;Siqueira, L.G.B. ................................................................................................................................... 377

EFFECT OF DIFFERENT LEVELS OF METABOLIZABLE ENERGY ON TESTICULARHISTOLOGY OF DEVELOPING SANTA INÊS RAMS Assis, R.M.; Perez, J.O.;Souza, J.C.; Barreto Filho, J.B. ........................................................................................................... 378

EVALUATION OF SPERM HETEROGENEITY OF SHEEP SANTA INES THROUGHCOMPUTERIZED ANALYSIS MORFOMETRIC NOT AUTOMATIZED Monteiro, C.D.;Bicudo, S.D.; Toma, H.S.; Azevedo, H.C.; Rodello, L.; Sicherle, C.C. ............................................. 379

Abstracts - Artificial Insemination and FTAI

DOSES OF ESTRADIOL CIPIONATE AS OVULATORY STIMULUS IN A TIMED ARTIFICIALINSEMINATION PROTOCOL Meneghetti, M.; Losi, T.C.; Vilela, E.R., Maia, S.A.;Vasconcelos, J.L.M. ............................................................................................................................ 383

EFFECT OF GNRH ON PREGNANCY RATES IN EWES SHORT PROGESTAGENSSYNCHRONIZATION ESTROUS Cavalcanti, A.S.; Fonseca, J.F.; Nogueira, L.A.G.;Brandão, F.Z.; Silva, A.L.S.; Pinho, T.G.; Pinna, A.E.; Carvalho, B.C. ............................................. 384

OVULATION RATE IN EWES SYNCHRONIZATION ESTRUS WITH SHORTPROGESTAGENS AND GNRH TREATMENT Cavalcanti, A.S.; Fonseca, J.F.; Nogueira, L.A.G.;Brandão, F.Z.; Silva, A.L.S.; Pinho, T.G.; Boité, M.C.; Carvalho, B.C. ............................................. 385


XIX

FOLLICULAR DYMANICS AND FERTILITY IN BEEF SUCKLED COWS SYNCHRONIZEDWITH PROGESTERONE RELEASING DEVICES AND GNRH Pincinato, D.; Peres, L.C.;Miranda, G.; Cutaia, L.; Bó, G.A. ........................................................................................................ 386

INFLUENCE OF UTERINE DIAMETER, OVARIAN SIZE AND OVARIAN STRUCTURESON FERTILITY IN 15 MONTH OLD CROSS-BRED BONSMARA HEIFERS INSEMINATEDAL FIXED-TIME Cutaia, L.; Peres, L.; Pincinato, D.; Bó, G.A. ....................................................... 387

EFFECT OF THE TIME OF INSEMINATION AND GNRH TREATMENT ON PREGNANCYRATES IN BEEF SUCKLED COWS SYNCHRONIZED WITH CO-SYNCH PROGRAMS WITHPROGESTERONE RELEASING DEVICES Pincinato, D.; Peres, L.C.; Chesta, P.; Martinez, M.;Cutaia, L.; Bó, G.A. ............................................................................................................................. 388

EFFICIENCY OF AN UNDER DOSE OF CLOPROSTENOL SODIC GIVEN BY SUB MUCOUSVULVAR INJECTION FOR INDUCTION OF ESTRUS OF FEMALE BUFFALOES ATDIFFERENT PERIODS OF POSTPARTUM Bartolomeu, C.C.; Del Rei, A.J.; Neves, A.L.A.;Balbino, S.C.; Oliveira, L.C.; Álvares, C.T. ....................................................................................... 389

EFFECTS OF EXOGENOUS PROGESTERONE, IN PRESENCE OR ABSENCE OF A CORPUSLUTEUM, IN THE OVARIAN FOLLICULAR DYNAMICS NELLORE HEIFERS Cipriano,R.S.; Carvalho, B.A.; Marangoni, N.R.; Nogueira, G.P. ..................................................................... 390

CIPIONATE OU ESTRADIOL BENZOATE AT THE DEVICE OF PROGESTERONEINSERTION AT RESPONSE TO A TIMED ARTIFICIAL INSEMINATON PROTOCOLMeneghetti, M.; Losi, T.C.; Vilela, E.R.; Martins Jr., A.P. ; Vasconcelos, J.L.M. ............................. 391

ADDITION OF eCG OR CALF REMOVAL AT THE END OF A TIMED ARTIFICIALINSEMINATION PROTOCOL Meneghetti, M.; Losi, T.C.; Vilela, E.R., Vilela Neto, J.M.;Vasconcelos, J.L.M. ............................................................................................................................ 392

EFFECT OF OESTRADIOL CYPIONATE BEFORE INDUCTION OF OVULATION ONSUBSEQUENT LUTEAL LIFESPAN IN ANOESTROUS NELORE COWS Sá Filho, O.G.;Vasconcelos, J.L.M. ............................................................................................................................ 393

ACUTE AND CHRONIC REDUCTION IN PLASMA PROGESTERONE CONCENTRATIONSAFTER FEED INTAKE IN HOLSTEIN DAIRY COWS Santos, R.M.; Perez, G.C.;Demétrio, D.G.B.; Vasconcelos, J.L.M. .............................................................................................. 394

EFFECTS OF AMOUNT OF CONCENTRATE ON DIET OF RECIPIENT HEIFERS ON SERICPROGESTERONE AND ON CONCEPTION RATE Santos, R.M.; Dias, C.C.; Ferreira Jr., N.;Vasconcelos, J.L.M. ............................................................................................................................ 395

INFLUENCE OF THE FLUNIXIN MEGLUMINE (BANAMINE®) AFTER FIXED TIMEARTIFICIAL INSEMINATION ON THE PREGNANCY RATE OF BEEF HEIFERSPfeifer, L.F.M.; Schneider, A.; Wilson Neto, J.; Castilho, E.M.; Dionello, N.J.L.;Corrêa, M. N. ....................................................................................................................................... 396

INFLUENCE OF THE APPLICATION OF FLUNIXIN MEGLUMINE (BANAMINE®) ONEHOUR BEFORE INSEMINATION ON THE PREGNANCY RATE OF EWES Pfeifer, L.F.M.;Meneghelo, L.; Wilson Neto, J.; Schneider, A.; Castilho, E.M.; Dionello, N.J.L.; Severo, N.C.;Rabassa, V.R.; Corrêa, M.N. ............................................................................................................... 397


XX

ULTRASOUND EVALUATION AND ESTRADIOL DOSAGE IN DAIRY COWS SUBMITTEDTO THE OVSYNCH PROTOCOL USING FERTIRELIN ACETATE AS ANALOG TO GNRHGioso, M.M.; Fernandes, C.A.C.; Oba, E.; Carvalho, M.A.P.; Vasconcelos, T.D.;Oliveira, E.R. ....................................................................................................................................... 398

EFICIENCY OF FERTIRELIN ACETATE APLICATION IN THE SYNCHRONIZATION OFFOLLICULAR EMERGENCE, OVULATION AND PREGNANT RATES IN DAIRY COWSSUBMITTED TO THE OVSYNCH PROTOCOL Gioso, M.M., 2; Fernandes, C.A.C.; Oba, E.;Carvalho, M.A.P.; Vasconcelos, T.D.; Oliveira, E.R. ......................................................................... 399

EFFECT OF TIME OF PERMANENCE OF A EAR PROGESTAGEN IMPLANT ON THEPREGNANCY RATE OF COWS SYNCHRONIZED FOR FIXED TIME ARTIFICIALINSEMINATION Marques, M.O.; Silva, R.C.P.; Ayres, H.; Souza, A.H.; Baruselli, P.S. ................. 400

CONCEPTION RATE IN SUCKLING NELORE COWS SYNCHRONIZED WITH AINTRAVAGINAL PROGESTERONE DEVICE ASSOCIATED WITH ESTRADIOL BENZOATEOR ESTRADIOL CYPIONATE Penteado, L.; Ayres H.; Torres-Júnior, J.R.; Souza, A.H.;Baruselli, P.S. ...................................................................................................................................... 401

PREGNANCY RATE IN NELORE COWS SUBMITTED TO TIMED INSEMINATION INDIFFERENT POSTPARTUM PERIODS Penteado, L.; Marques, M.O.; Silva, R.C.P.;Ayres, H.; Souza, A.H.; Baruselli, P.S. ................................................................................................ 402

EFFECT OF CYCLICITY INDUCTION IN NELORE HEIFERS TREATED WITH APREVIOUSLY USED INTRAVAGINAL PROGESTERONE DEVICE ON CONCEPTION RATEAFTER FIXED TIME ARTIFICIAL INSEMINATION Sá Filho, M.F.; Ayres, H.; Rezende, L.F.C.;Penteado, L.; Nasser, L.F.; Souza, A.H.; Baruselli, P.S. ..................................................................... 403

EFFECTS OF eCG AND ESTRADIOL CYPIONATE IN PROTOCOLS FOR FIXED TIMEARTIFICIAL INSEMINATION IN HIGH PRODUCING HOLSTEIN COWS Souza, A.H.;Martins, C.M.; Torres-Júnior, J.R.S.; Ayres, H.; Baruselli, P.S. ......................................................... 404

CERVICAL ARTIFICIAL INSEMINATION WITH FROZEN-THAWED SEMEN IN EWESIsobe, F.R.; Silva, B.J.C.; Severo, N.; Baptista, A.L.; Santos, I.C.C.; Oliveira, R.; Schneider, C.;Traldi, A.S. .......................................................................................................................................... 405

VIABILITY TWO UTERINE WASHING IN SEVENTH AND EIGTH DAYS AFTER AI INNELORE COWS Wünsche Jr., A.G.; Barreiros, T.R.R.; Bezerra, G.A.; Azevedo, J.R.;Oliveira, A.C.S.; Seneda, M.M. .......................................................................................................... 406

THE HYPOOSMOTIC SWELLING TEST IN FRESH GOAT SEMEN OF THE BOER BREEDAlves, S.G.G.; Chalhoub, M.; Bittencourt, R.F.; Biscarde, C.E.; Almeida, A.K.; Portela, A.P.M.;Vasconcelos, M.F.; Freitas, D.S.; Ribeiro Filho, A. de L. .............................................................. 407

EFFECT OF THE GNRH AND TIME OF ARTIFICIAL INSEMINATION ON CONCEPTIONRATE IN POSTPARTUM COWS SYNCHRONIZED WITH NORGESTOMET FOR FIXEDTIME ARTIFICIAL INSEMINATION Ayres, H.; Torres-Júnior, J.R.S.; Penteado, L.; Souza, A.H.;Baruselli, P.S. ...................................................................................................................................... 408

EFFECT OF GNRH TREATMENT AND TIME OF INSEMINATION ON CONCEPTION RATEOF POSTPARTUM COWS SYNCHRONIZED WITH A PREVIOUSLY USED NORGESTOMETIN PROTOCOL FOR FIXED TIME ARTIFICIAL INSEMINATION Ayres, H.;Torres-Júnior, J.R.S.; Penteado, L.; Souza, A.H.; Baruselli, P.S. ....................................................... 409


XXI

CONCEPTION RATE IN SUCKLING NELORE COWS SYNCHRONIZED WITHPROGESTAGEN EAR IMPLANT ASSOCIATED WITH ESTRADIOL BENZOATE ORCYPIONATE Ayres H.; Penteado, L.; Torres-Júnior. J.R.; Souza, A.H.; Baruselli, P.S. ................... 410

FIXED TIME ARTIFICIAL INSEMINATION (FTAI) USING HORMONAL PROTOCOLS,WITH OR WITHOUT EXOGENOUS PROGESTERONE, ASSOCIATED OR NOT TOTEMPORARY CALF REMOVAL (TCR) Souza, A.F.; Pinheiro, V.G.; Ereno, R.L.;Trinca, L.A.; Barros, C.M. .................................................................................................................. 411

EMBRYO AND RECIPIENT FACTOS THAT AFFECT CONCEPTION RATES OFCONVENCIONAL COLECTED AND IVF EMBRYOS TRANSFERS Dias, C.C.; Alvin, M.T.T.;Saliba, W.P.; Vasconcelos, J.L.M. ....................................................................................................... 412

FACTORS AFFECTING CONCEPTION RATE IN LACTATING HOLSTEIN RECIPIENTSJardina, D.T.G.; Saoncella, C.; Rodrigues, C.A.; Vasconcelos, J.L.M. .............................................. 413

CONCEPTION RATES AT EMBRYO TRANSFER IN LACTATING DAIRY RECIPIENT COWSAFTER ESTROUS OR OVULATION SYNCHRONIZATION Jardina, D.T.G.; Santos, R.M.;Demétrio, D.G.B.; Rodrigues, C.A.; Vasconcelos J.L.M. ................................................................... 414

REPRODUCTIVE EFFICIENCY OF NELORE COWS SUBMITED TO DIFFERENT KINDOF MANAGEMENT IN RIO GRANDE DO NORTE’S AGRESTE REGION Rocha, J.M.; Rabelo,M.C.; Santos, M.H.B.; Moraes, E.P.B.X.; Chiamenti, A.; Falcão, D.P.; Lima, P.F.;Oliveira, M.A.L. .................................................................................................................................. 415

AIFT IN NELORE COWS: REDUCTION OF eCG DOSE AND REUTILIZATION OFINTRAVAGINAL DEVICE OF PROGESTERONE Rocha, J.M.; Rabelo, M.C.; Santos, M.H.B.;Falcão, D.P.; Moraes, E.P.B.X.; Bartolomeu, C.C.; Lima, P.F.; Oliveira, M.A.L. ............................. 416

EFFECT THE TREATMENT WITH CIDR, ESTRADIOL BENZOATE AND FSH IN NELORECOWS WITH DECREASED OOCYTES RECOVER IN THE IN VITRO PRODUCEDEMBRYOS RATIO Borsato, E.A.; Silva, D.R.M.; Barreiros, T.R.R.; Blaschi, W.; Seneda, M.M. ..................................................................................................................................... 417

FOLLICULAR STANDARD ON REPEAT BREEDERS BEEF COWS UNDER OVULATIONSINCHRONIZATION WITH NORGESTOMET AND ESTRADIOL BENZOATEBarreiros, T.R.R.; Blaschi, W.; Antoniazzi, A.Q.; Bassani, C.A.; Seneda, M.M. .............................. 418

INFLUENCE OF NUTRITIONAL DIET DENSITY ON CONCEPTION RATE IN NELLORECOWS Losi, T.L.; Meneghetti, M.; Vilela, E.R.; Santos, R.M.; Reis, A.M.; Vasconcelos, J.L.M. ... 419

FACTORS AFFECTING PREGNANCY RATES IN BEEF COWS TREATED WITH CIDRDEVICES AND TIMED INSEMINATED Valarelli, R.L.; Barbarini, O.; Vasconcelos, J.L.M. ....... 420

COMPARISON BETWEEN DIFFERENT CONCENTRATIONS OF FRESH DONKEY (EQUUSASINUS) SEMEN FOR ARTIFICIAL INSEMINATION OF DONKEY MARES Oliveira, J.V.;Papa, F.O.; Alvarenga, M.A.; Carmo, M.T.; Araújo, G.H.M.; Santos,J.F. .......................................... 421

SYNCHRONIZATION OF OVULATION OF MURRAH FEMALE BUFFALOES TREATEDWITH INTRAVAGINAL DEVICE (CRONIPRESS) ASSOCIATED OR NOT TO eCG IN TWODISTINCT REPRODUCTIVE STATIONS Del Rei, A.J.; Bartolomeu, C.C.; Neves, A.L.A.;Balbino, S.C.; Oliveira, L.C.; Álvares, C.T. ....................................................................................... 422


XXII

EFFECT OF DIFFERENT PROTOCOLS ON PREGNANT RATE OF NELORE COWSINSEMINATED IN FIXED TIME Câmara, D.R.; Figueira, R.F.; Mendonça, L.B.R.; Pinto, L.C.;Mello, M.M.C.; Paiva, D.S. ................................................................................................................ 423

EFFECT OF DIFFERENT DOSIS OF FERTIRELIN TO INDUCE OVULATION IN FIXED-TIME INSEMINATION PROTOCOLS IN SUCKLED BEEF COWS Oliveira, G.R.;Ogando, P.P.; Bacchin, A.B.; Borges, J.B.S. ....................................................................................... 424

OSMOTIC STRESS EVALUATION OF STALLION SPERM EXPOSED TO HIPERTONICSOLUTION OF DIFERENTS CRYOPROTECTANTS Medeiros, A.S.L.; Papa, F.O.;Araujo, G.H.M.; Freitas, C.P.; Alvarenga, M.A. ................................................................................. 425

EFFECT OF CALVING MONTH ON PREGNANCY RATES OF BEEF COWSSYNCHRONIZED FOR FIXED TIME ARTIFICIAL INSEMINATION Penteado, L.;Marques, M.O.; Silva, R.C.P.; Ayres, H.; Souza, A.H.; Baruselli, P.S. .............................................. 426

CONCEPTION RATE IN LACTATING NELORE COWS SYNCHRONIZED WITH ANINTRAVAGINAL PROGESTERONE DEVICE ASSOCIATED WITH DIFFERING TIMES OFESTRADIOL BENZOATE TREATMENT AND FIXED TIME ARTIFICIAL INSEMINATIONMartins, C.M.; Mello, J.E.; Dominguez, J.H.; Ayres, H.; Souza, A.H.; Baruselli, P.S. ..................... 427

TIMED ARTIFICIAL INSEMINATION PROTOCOLS IN CROSSBRED DAIRY COWS Cardoso, B.L.; Pescara, J.B.; Vasconcelos, J.L.M. ............................................................................ 428

Abstracts - IVF

MITOCHONDRIAL MEMBRANE POTENTIAL BLOCKAGE OF IN VITRO BOVINEEMBRYOS Mesquita, L.G.; Cortezzi, S.S.; Ripamonte, P.; De Bem, T.H.C.; Porciuncula, P.M.;Merighe, G.K.F; Balieiro, J.C.C.; Leal, C.L.V.; Meirelles, F.V. ......................................................... 431

IN VITRO MATURATION OF OOCYTES OBTAINED FROM NELORE COWS (Bos taurusindicus) WITH AND WITHOUT DOMINANT FOLLICLE – PARTIAL RESULTS Leal, L.S.;Fernandes, C.B.; Melo, D.S.; Assaf, S.S.; Oba, E.; Landim-Alvarenga, F.C. .................................... 432

EFFECT OF DIFFERENTS CAPACITATION AGENTS IN IN VITRO PRODUCTION OFBOVINE EMBRYOS USING SEXED SPERM Accorsi, M.F.; José, A.A.F.B.V.; Watanabe, M.R.;Joaquim, D.C.; Meirelles, F.V.; Watanabe, Y.F. .................................................................................. 433

CORTICAL GRANULE DISTRIBUTION IN BOVINE OOCYTES MATURED IN VITRO WITHNUCLEAR MATURATION INHIBITORS AND ANTIOXIDANTS Barretto, L.S.S.;Gonçalves, F.S.; Perri, S.H.V.; Mingoti, G.Z. ..................................................................................... 434

GLUTATHIONE INTRACELULAR CONCENTRATION IN BOVINE OOCYTES MATUREDIN VITRO WITH NUCLEAR MATURATION INHIBITORS AND ANTIOXIDANTSBarretto, L.S.S.; Tetzner, T.A.D.;Gonçalves, F.S.; Mingoti, G.Z. ....................................................... 435

USE OF GLUTAMAXTM-I AS SUBSTITUTE TO L-GLUTAMINE IN CULTURE MEDIUMOF BOVINE EMBRYOS: PRELIMINARIES RESULTS Elias, F.P.; Vila, R.A.; Galerani, M.A.V.;Fernandes, M.B.; Lôbo, R.B. .............................................................................................................. 436

ASSOCIATION OF BOVINE SPERM MOTILITY AND VIABILITY WITH IN VITRO EMBRYOPRODUCTION Dias, N.B.; Camargo, L.S.A., Serapião, R.V.; Folhadela, I.;Ramos, A.A.; Polisseni, J.; Viana, J.H.M.; Sá, W.F.; Ferreira, A.M................................................... 437


XXIII

LARGE SCALE COMMERCIAL APPLICATION OF IN VITRO PRODUCED EMBRYOSEXING USING DNA ANALYSIS Alonso, R.V.; Hellú, J.A.A.; Ardais, D.B.; Visintin, J.A.;Garcia, J.F. ........................................................................................................................................... 438

ADDITION OF ASCORBIC ACID TO THE CULTURE MEDIUM OF IN VITRO PRODUCEDBOVINE EMBRYOS Silva, C; Calegari, R.S.; Martins Jr., A. .......................................................... 439

DEVELOPMENT OF THE ALLANTOIS BOVINE OBTAIDED BY GESTATIONS IVPAssis Neto, A.C.; Verechia, F.T.V.; Ambrósio, C.E.; Meirelles, F.V.; Miglino, M.A2. ...................... 440

IN VITRO NUCLEAR MATURATION OF EQUINE OOCYTES ACCORDING TO THE STATUSOF COMPACTATION OF GRANULOSA AND CUMULUS CELLS Fernandes, C. B.; Lima-Neto, J.F.; Martins, L.R.; Bonesi, G.L.; Tsuribe, P.M.; Melo, C.M.; Leal, L.S.; Dores, C.B.; Blanco,I.D.P.; Derussi, A.A.P.; Pavão, G.D.; Martin, I.; DeVitto, L.G 1; Landim-Alvarenga, F.C. ............... 441

OPU OF FEMALE BUFFALOES TREATED WITH RECOMBINANT BOVINESOMATOTROPIN DURING THEIR ANESTROUS SEASON PERIOD Miranda, M.S.; Almeida,A.B.; Chiaratti, M.R.; Balieiro, J.C.C.; Baruselli, P.S.; Meirelles, F.V.; Ohashi, O.M. ..................... 442

INHIBITION AND REVERSIBILITY OF THE BOVINE OOCYTE NUCLEAR MATURATIONSandri, L.R.; Bohrer, R.C.; Ferreira, R.; Gonçalves, P.B.D.; Oliveira, J.F.C. .................................... 443

EFFECT OF NITRIC OXIDE INHIBITION ON THE IN VITRO DEVELOPMENT OF BOVINEEMBRYOS:PRELIMINARY RESULTS. Schwarz, K.L.; Pires, P.R.L.; Adona, P.R.;Leal, C.L.V. ......................................................................................................................................... 444

NUCLEAR CHROMATIN KINETICS DURING IN VITRO MATURATION OF BOVINEOOCYTES MANTAINED IN TRANSPORTATION MEDIUM - MIV-T Leme, L.O.; Rumpf, R.;Dode, M.A.N. ...................................................................................................................................... 445

EFFECT OF GONADOTROPINS DURING IN VITRO MATURATION ON BOVINE EMBRYOPRODUCTION Leme, L.O.; Driessen, K.; Rumpf, R.; Dode, M.A.N. ............................................ 446

EFFECT OF THE USE OF ESTRADIOL BENZOATE AND PROGESTERONE FORFOLLICULAR WAVE SYNCHRONIZATION AIMMING IN VIVO OOCYTE RECOVERY.PRELIMINARY RESULTS Ramos, A.F.; Sartori, R.; Siqueira Filho, E.; Mollo, M.R.;Driessen, K.; Pivato, I.; Marques Jr., A. P., Rumpf, R. ....................................................................... 447

GENE EXPRESSION ANALYSIS ON IN VITRO PRODUCED EMBRYOS FROM OOCYTESOBTAINED OF COWS SUBMITED TO DIFFERENT LEVELS OF NUTRITION. Martins, A.C.;Mundim, T.C.D.; Melo, E.O.; Dode, M.A.N.; Rumpf, R.; Franco, M.M.; Sartori, R. ....................... 448

EVALUATION OF THE LARGE OFFSPRING SYNDROME (LOS) OCCURRENCE IN THEREGION NORTH OF THE PARANÁ. Ruza, R.G.; Baudraz, J.A.; Santos, E.S.;Melo Sterza, F.A. ................................................................................................................................. 449

USE OF EXOGEN PROGESTERONE ON OOCYTE RECOVERY OBTAINED BY OVUMPICK-UP (OPU), EMBRYO PRODUCTION AND PREGNANCY IN NELORE FEMALES –PRELIMINARY RESULTS Nonato Jr., I.; Pontes, J.H.F.; Ereno Jr., J.C.; Gimenes, L.U.G.;Torres-Júnior, J.R.S.; Baruselli, P.S. ................................................................................................... 450


XXIV

NUCLEAR MATURATION KINETICS OF BOVINE OOCYTES AFTER MEIOSIS BLOCKIN VITRO WITH DIFFERENT CONCENTRATIONS OF BUTYROLACTONE I. Quetglas, M.D.;Adona, P.R..; Leal, C.L.V. ................................................................................................................... 451

EMBRYONIC DEVELOPMENTAL COMPETENCE OF BOVINE OOCYTES MATURED INCHEMICALLY DEFINED MEDIUM SUPPLEMENTED WITH SYNTHETICMACROMOLECULES Vireque, A.A.; Ferreira, E.M.; Ferriani, R.A.; Navarro, P.A.A.S.;Rosa e Silva, A.A.M.; Watanabe, Y.F. ................................................................................................. 452

BOVINE IN VITRO FERTILIZATION WITH CYSTEAMINE, 2-MERCAPETHANOL ANDHEPARIN Gonçalves, F.S.; Barretto, L.S.S.; Perri, S.H.V.; Mingoti, G.Z. ........................................ 453

EFFECT OF ANTIOXIDANTS IN THE BOVINE IN VITRO FERTILIZATION MEDIUM ONTHE EMBRYONIC QUALITY Gonçalves, F.S.; Barretto, L.S.S.; Perri, S.H.V.; Mingoti, G.Z. ...... 454

EFFECT OF ANTIOXIDANTS ON THE BOVINE SPERM MEMBRANE DURING IVF:EVALUATION BY FLUORESCENTS PROBES Gonçalves, F.S.; Barretto, L.S.S.; Arruda, R.P.;Perri, S.H.V.; Mingoti, G.Z. ................................................................................................................. 455

EFFECT OF GONADOTROPHIN TREATMENT ON THE EFFICIENCY OF LAPAROSCOPICOVUM PICK-UP IN GOATS: PRELIMINARY RESULTS Magalhães, D.M.; Pereira, A.F.;Andrade, M.L.L.; Cajazeiras, J.B.; Lopes Júnior, E.S.; Freitas, V.J.F. ............................................. 456

EVALUATION OF NUCLEAR MATURATION OF HORSE OOCYTES WITH COMPACT ANDEXPANDED CUMULI, IN AN ATMOSPHERE WITH MIXED GAS Curcio, B.R.; Leite, D.S.P.;Boff, A.N.; Velho, J.; Rambo, G.; Nogueira, C.E.W.; Deschamps, J.C. ............................................. 457

EFFECTS OF FERTILIZATION MEDIA AND SPERM/OOCYTE INCUBATION PERIODS ONIN VITRO PRODUCTION OF SWINE EMBRYOS Marques, M. G.; Goissis, M.D; Nascimento,A.B.; Peres, M.A.; Gonçalves, J.S.A.; Feitosa, W.B.; Assumpção, M.E.O.A.; Visintin, J.A. ........... 458

OOCYTE RECOVERY OF NELORE CATTLE USING A HORMONAL PROTOCOL WITHINJECTABLE PROGESTERONE Bacelar, D.; Padilha, L.C.; Baruselli, P.S.; Seneda, M.M. ......... 459

MODIFIED LAPAROSCOPIC TECHNIQUE FOR RECOVERY OF OOCYTES IN YOUNGGOATS Cordeiro, M.F.; Di Filippo, P.A.; Dória, R.G.S.; Dias, D.P.M.; Beretta, C.A.G.; Oliveira,M.E.F.; Oliveira Jr., E.G.; Ribeiro, G.; Alves, A.E.; Duarte, A.L.L.; Vicente, W.R.R. ....................... 460

EFFECT OF THE BULL ON IN VITRO EMBRYO PRODUCTION, PREGNANCY ANDEMBRYONIC DEATH RATES. PRELIMINARY RESULTS Pontes, J.H.F.; Spegiorin, M.R.;Silva, K.C.F.; Seneda, M.M.. .............................................................................................................. 461

BOVINE EMBRYOS CULTURED IN SERUM-FREE MEDIUM IN DIFFERENTATMOSPHERIC CONDITIONS Serapião, R.V.; Ramos, A.A.; Polisseni, J.; Folhadella, I.M.;Dias. N.B.; Camargo, L.S.A.; Viana, J.H.M.; Ferreira, A.M.; Sá, W.F.; Fonseca, F.A. ..................... 462

EFFECT OF OOCYTE MORPHOLOGICAL QUALITY ON EMBRYO DEVELOPMENT INVITRO De Bem, T.H.C.; Biase, F.H.; Bortolotti, G.M.F.; Adona, P.R.; Meirelles, F.V.;Leal, C.L.V. ......................................................................................................................................... 463

EFFECT OF MEIOSIS BLOCK ON IN VITRO EMBRYO DEVELOPMENT Adona, P.R.;De Bem, T.H.C.; Balieiro, J.C.C.; Leal, C.L.V. .................................................................................. 464


XXV

DNA FRAGMENTATION IN BOVINE EMBRYOS PRODUCED IN VITRO FROM OOCYTESOF DIFFERENT MORPHOLOGICAL QUALITIES De Bem, T.H.C.; Mesquita, L.G.;Miranda, M.S.; Adona, P.R.; Meirelles, F.V.; Leal, C.L.V. ................................................................. 465

IMATURE OOCYTES TRANSPORTATION AND ITS EFFECT ON IN VITRO PRODUCTIONOF BOVINE EMBRYOS Leme, L.O.; Marinheiro, G.O.; Sousa, R.V.; Dode, M.A.N.;Rumpf, R. ............................................................................................................................................ 466

IN VITRO PRODUCTION OF BOVINE EMBRYOS UNDER HIGH AND LOW O2 TENSIONCorrea, G.A.; Dode, M.A.; Franco, M.M.; Rumpf, R. ........................................................................ 467

EFFECT OF ESTRADIOL BENZOATE, PROGESTAGENS AND OVUM PICK-UP ONFOLLICULAR WAVE SYNCHRONIZATION AND FSH CONCENTRATION IN OOCYTEDONNOR HEIFERS Ramos, A.F.; Rumpf, R.; Mollo, M.R.; Pivato, I.; Nogueira, G.P.;Marques Jr, A.P.; Sartori, R. ................................................................................................................ 468

EVALUATION OF THE COCONUT WATER SOLUTION ON THE MAINTENANCE OFIMMATURE BOVINE OOCYTES FOR IN VITRO EMBRYO PRODUCTION Cordeiro, M.S.;Silva, E.H.S.; Biondi, F.C.; Miranda, M.S.; Andreoti, M.; Carvalho, C.M.F.; Silva, T.V.G.;Costa, N.N.; Santos, S.S.D.; Ohashi, O.M. ......................................................................................... 469

EFFECT OF PRESENCE OF CORPUS LUTEUM AT THE TIME OF FOLLICULARASPIRATION ON OOCYTE RECOVERY AND IN VITRO COMPETENCE IN Bos indicusCATTLE Torres-Júnior, J.R.S.; Pires, M.F.Á.; Sá, W.F.; Ferreira, A.M.; Viana, J.H.M.; Camargo,L.S.A.; Ramos, A.A.; Folhadella, I.M.; Polisseni, J.; Freitas, C.; Clemente, C.A.A.;Sá Filho, M.F.; Souza, A.H.; Baruselli, P.S. ....................................................................................... 470

EFFECT OF FOLLICULAR CODOMINANCE AT THE TIME OF FOLLICULAR ASPIRATIONON THE RECOVERY AND IN VITRO COMPETENCE OF OOCYTES IN Bos indicus CATTLETorres-Júnior, J.R.S.; Pires, M.F.Á.; Sá, W.F.; Ferreira, A.M.; Viana, J.H.M.; Camargo, L.S.A.;Ramos, A.A.; Folhadella, I.M.; Polisseni, J.; Freitas, C.; Clemente, C.A.A.; Sá Filho, M.F.;Souza, A.H.; Baruselli, P.S. ................................................................................................................. 471

EFFECTIVENESS OF 30 AND 36-HOUR IN VITRO NUCLEAR MATURATION OFDOMESTIC CAT OOCYTES Martins, L.R.; Fernandes, C.B.; Landim-Alvarenga, F.C.;Lopes, M.D. ......................................................................................................................................... 472

COMPARISON AMONG DIFFERENT MEDIA FOR SWIM-UP OF SPERMATOZOA FROMCAUDA EPIDIDYMIDES AND VASA DEFERENTIA OF NORMOSPERMIC DOMESTICCATS (Felis catus) Martins, L.R.; Villaverde, A.I.S.B.; Carnicelli, P.; Taconeli, C.A.;Landim-Alvarenga, F.C.; Lopes, M.D. ................................................................................................ 473

INFLUENCE OF HEAT STRESS ON APOPTOSIS RATE OF IN VITRO PRODUCE BOVINEEMBRYOS (Indicus vs Taurus) AND IN THEIR CAPACITY TO ORIGINATE PREGNANCIESSartorelli, E.S.a; Satrapa, R.A.a; Barcelos, A.C.Z.b; Potiens, J.R.; Barros, C.M. ............................. 474

FOLLICULAR ASPIRATION IN NELLORE COWS: EFFECT OF DONOR AGE AND SEASONON OOCYTE PRODUCTION AND QUALITY Calegari, R.S.; Paschoal, D.M.;Martins Jr., A. ...................................................................................................................................... 475

COMPARISON OF TWO VITRIFICATION PROTOCOLS FOR IN VITRO PRODUCEDBOVINE EMBRYOS Oliveira, R.S.; Dias, A.J.B.; Sá, W.F.; Camargo, L.S.A.; Viana, J.H.M.;Moraes, G.A. ........................................................................................................................................ 476


XXVI

EFFECT OF FOLLICULAR FLUID AND dbcAMP ON THE IN VITRO PRODUCTION OFSWINE EMBRYO Nascimento, A.B.; Marques, M.G.; Gerger, R.P.C.; Yamada, C.; Peres, M.A.;Assumpção, M.E.O.A.; Visintin, J.A.; Bordignon, V. ........................................................................ 477

FETAL BOVINE SERUM IMPROVE EMBRYO QUALITY FROM OOCYTES MATURED INTCM199 ADDED dbcAMP AND PORCINE FOLLICULAR FLUID? Nascimento, A.B.; Nicacio,A.C.; Marques, M.G.; Tavares, M.N.; Gonçalves, J.S.A.; Assumpção, M.E.O.A.; Visintin, J.A.;Bordignon, V. ....................................................................................................................................... 478

USE OF DIFFERENT CONCENTRATIONS OF FETAL BOVINE SERUM AND PORCINEFOLLICULAR FLUID ON THE SWINE EMBRYO CULTURE IN VITRO PRODUCEDNascimento, A.B.; Goissis, M.D.; Marques, M.G.; Gonçalves, J.S.A.; Tavares, M.N.;Assumpção, M.E.O.A.; Visintin, J.A.; Bordignon, V. ........................................................................ 479

IN VITRO PRODUCTION OF BOVINE EMBRYOS IN PRESENCE OF SIGMA´S®

ANTIOXIDANT Sudano, M.J.; Mattos M.C.C.; Fernandes C.B.; Lima Neto J.F.;Landim-Alvarenga, F.C. ...................................................................................................................... 480

USE OF SEXED SEMEN ON IN VITRO FERTILIZATION OF CATTLE OOCYTESPonchirolli, C.B.; Fonseca, E.J.; Teobaldo-Filho, O.; Moreno, J.F. ................................................... 481

USE OF HUMAN COMERCIAL MEDIUM TO THE DEVELOPMENT OF IN VITRO BOVINEEMBRYOS: EARLY RESULTS Lima, T.N.; Benedetti, E.R.; Prado, R.B.; Atique-Netto, H. .......... 482

SOF VS TCM 199: INFLUENCE ON THE IN VITRO DEVELOPMENT OF BOVINE EMBRYOSAFTER CRYOPRESERVATION Nicacio, A.C.; Simões, R.; Nascimento, A.B.; Milazzotto, M.P.;Delboni, C.C.; Goissis, M.D.; Assumpção, M.E.O.A.; Visintin, J.A. ................................................ 483

EFFECT OF DIFFERENT CRYOPROTECTANT ON THE VIABILITY OF IN VITROPRODUCED BOVINE EMBRYOS Delboni, C.C.; Nicacio, A.C.; Feitosa, W.B.; Nascimento,A.B.; Simões, R.; Milazzotto, M.P.; Assumpção, M.E.O.A.; Visintin, J.A. ....................................... 484

TEST OF TWO ESPERMATIC CAPACITATION AGENTS FOR IN VITRO PRODUCTION OFSHEEP EMBRYOS USING OOCYTES RECOVERY BY LAPAROSCOPY FROM SANTA INÊSDONORS Panattoni, J.F.; Oliveira, P.C.; Figueiredo, C.L.; Pontes, J.H.F.; Alves Jr, S.S.;Sala, R.V.; Jorge, R.S. ......................................................................................................................... 485

APOPTOSIS AND NECROSIS QUANTIFICATION IN ZEBUINE EMBRYOS PRODUCED INVITRO AND VITRIFIED IN SOLUTIONS CONTAINING CARBOHIDRATES OR NOTMachado, V.C.; Dias, A.J.B.; DaMatta, R.A.; Paes de Carvalho, C.S.; Thiebaut, J.T.L. ................... 486

REPORT OF BOVINE IN VITRO EMBRYO PRODUCTION FROM SEXED SEMENAvelino, K.B.; Basso, A.C.; Rossetto, M.R.; Maraia, A.C.; Landim Jr., L.P.; Garcia, J.M. .............. 487

SEXED BOVINE SPERM: APPLICATION OF TWO DIFFERENT TECHNIQUES Basso, A.C.;Avelino, K.B.; Rossetto, M.R.; Maraia, A.C.; Landim Jr., L.P.; Garcia, J.M. .................................... 488

GENETICS AND ENVIRONMENTAL EFFECTS ON EMBRYO IN VITRO PRODUCTIONUSING FROZEN SEMEN OF YOUNG BULLS WITH PROGENY TEST Gavio, D.;Vozzi, P.A.; Fernandez, M.B.; Elias, F.P.; Vila, R.A.; Galerani, M.A.V.; Lôbo, R.B. ........................ 489


XXVII

ASSOCIATION OF IONOMYCIN, STRONTIUM AND 6-DIMETHYLAMINOPURINE INCHEMICAL ACTIVATION OF YOUNG BOVINE OOCYTES Porciúncula, P.M.; Bressan, F.F.;Cortezzi, S.S.; De Bem, T.H.C.; Leal, C.L.V.; Mesquita, L.G.; Merighe, G.K.F.,Miranda, M.S.; Meirelles, F.V ............................................................................................................ 490

Abstracts - Embryo Transfer

ESTRUS DETECTION EFFICIENCY IN EMBRYO RECIPIENTS SYNCHRONIZED WITHCLOPROSTENOL Fernandes, C.A.C.; Figueiredo, A.C.S.; Oliveira, E.R.; Vasconcelos, T.D.;Alves, B.F.L.; Gioso, M.M.; Viana, J.H.M.; Oba, E. .......................................................................... 493

LUTEAL TISSUE AREA IN EMBRYO RECIPIENTS SYNCHRONIZED AND WITHSPONTANEOUS OR ESTRADIOL BENZOATE INDUCED OVULATION Siqueira, L.G.B.;Viana, J.H.M.; Diniz, E.S.; Camargo, L.S.; Oliveira, A.P.; Fernandes, C.A.C.; Torres, C.A.A. ....... 494

PROBABILITY DENSITY FUNCTION OF THE NUMBER OF EMBRYOS COLLECTED INSUPEROVULATED NELORE COWS Beltrame, R.T.; Quirino, C.R.; Barioni, L.G. ...................... 495

IN VIVO EMBRYO PRODUCTION IN MOXOTÓ GOATS PARTICIPATING IN APRESERVATION PROGRAM Almeida, A.P.; Lima, I.M.T.; Paula, N.R.O.; Souza, A.L.;Andrade, M.L.L.; Lopes Júnior, E.S.; Andrioli, A.; Rondina, D.; Freitas, V.J.F. ............................... 496

EVALUATION OF THE EFFICIENCY OF SYNCHRONIZATION PROTOCOLS OF BOVINEEMBRYOS RECIPIENTS FOR TETF USING ESTRADIOL BENZOATE OR ESTRADIOLCYPIONATE. De Quadros, F.A.; Melo Sterza, F.A.; Santos, E.S.; Fonseca, A.; Baudraz, J.A.;Marques, V.T.; Ferri, F.; Marques, M.O.; Silva, R.C.P.; Baruselli, P.S. ............................................. 497

PREGNANCY RATE FOLLOWING TRANSFER OF EQUINE EMBRYOS STORED FORDIFFERENT PERIODS BETWEEN 15 AND 18º C IN VIGRO HOLDING MEDIAAlonso, M.A.; Fleury, P.D.C.; Neves Neto, J.R.; Squires, E.L. .......................................................... 498

β-CAROTENE AND TOCOPHEROL ON BOVINE EMBRYO PRODUCTION AND QUALITYSales, J.N.S.; Souza, J.C. .................................................................................................................... 499

INTRA-UTERINE TREATMENT FOR INFECTIONS IN COWS Canella Filho, C.C.; Fernandes,C.A.C.; Viana, J.H.M.; Figueiredo, A.C.S.-3; Gioso, M.M.; Ludgero, B.F.A.; Oliveira, E.R.;Vasconcelos, T.D. ................................................................................................................................ 500

EVALUATION OF THE RECIPIENT MARE: EFFECT OF UTERINE CHARACTERISTICSAND TIME OF OVULATION Fleury, P.D.C.; Alonso, M.A.; Balieiro, J.C.C. ................................ 501

PREGNANCY RATES AND PROGESTERONE CONCENTRATIONS ON RECIPIENTSIMPLANTED WITH IN VITRO PRODUCED BOVINE EMBRYOS AND SUPPLEMENTEDWITH BETACAROTENE ASSOCIATED TO TOCOPHEROL Sales, J.N.S.; Souza, J.C.;Dias, L.M.K.; Franci, C.R.; Rocha, A.A.; Cardoso, G.G.C. ................................................................ 502

RELATIONSHIP BETWEEN PRE AND POST-SUPEROVULATORY PROGESTERONECONCENTRATIONS AND EMBRYO PRODUCTION AND QUALITY IN HOLSTEINHEIFERS Sales, J.N.S.; Souza, J.C. ................................................................................................... 503

COMPARISON OF TWO PROTOCOLS FOR SYNCRONIZATION OF OVULATION INBOVINE EMBRYO RECIPIENTS Mollo, M.R.; Ramos, A.F.; Siqueira Filho, E.; Pivato, I.;Saueressig, M.G.; Rumpf, R.; Sartori, R. ............................................................................................ 504


XXVIII

SEX IDENTIFICATION OF IN VIVO PRODUCED BOVINE EMBRYOS USING PCR UNDERFIELD CONDITIONS Sousa, RV.; Cardoso, C.R.S.; Butzke, G.; Franco, M.M.; Rumpf, R. ........... 505

ECONOMIC EVALUATION OF BOVINE EMBRYO SPLITTING Fernandes, C.A.C.; Oba, E.;Viana, J.H.M.; Figueiredo, A.C.S.; Oliveira, E.R.; Vasconcelos, T.D.; Gioso, M.M. ........................ 506

COMPARATIVE STUDY OF THE PREGNANCY RATES AND EMBRYONIC DEATH RATESIN CYCLING AND NONCYCLING PROGESTERONE-TREATED RECIPIENT MARES Pinna,A.E.; Queiroz, F.J.R.; Greco, G.M.; Cavalcanti, A.S.; Boité, M.C.; Brandão, F.Z.; Pinho, T.G.;Nogueira, L.A.G.; Almeida, N.K.O. .................................................................................................... 507

SUPEROVULATION IN DOES BASED ON FIRST FOLLICULAR WAVE OF THE ESTROUSCYCLE Fonseca, J.F.; Bruschi, J.H.; Zambrini, F.N.; Viana, J.H.M.; Amorim, L.S.;Camargo, L.S.O. .................................................................................................................................. 508

INFLUENCE OF PLASMATIC PROGESTERONE PROFILES ON OVARIAN RESPONSE ANDEMBRYO PRODUCTION OF MORADA NOVA (VARIETY WHITE) EWES Lopes Júnior, E.S.;Maia, E.L.M.M.; Almeida, K.C.; Paula, N.R.O.; Teixeira, D.I.A.; Rondina, D.;Selaive-Villaroel, A.B.; Freitas, V.J.F. ................................................................................................ 509

EVALUATION OF DIFFERENT METHODS OF BOVINE EMBRYOS FLUSHINGPinto-Neto, A.; Demczuk, E.; Previato, P.F.G.; Mota, M.F.; Acco, A.; Fonseca, J.F. ......................... 510

RECUPARATION OF EQUINE FLHUSHING CATHETER AS A TOOL FOR COSTREDUCTION Gusmão, A.L.; Feitosa, T.A.L.; Silveira, L.L.; Freitas, M.V.V.; Resende, J.;Andrade Moura, J.C. ........................................................................................................................... 511

SEASON AND LACTATION PHASE IN EMBRYOS PRODUCTION IN HOLSTEIN DONORSJardina, D.T.G.; Rodrigues, C.A.; Guerreiro, V.J.; Vasconcelos, J.L.M. ............................................ 512

SUPERSTIMULATORY PROTOCOL P-36 IN BONSMARA BREED: USE OF eCG ANDDELAY ON INDUCTION OF OVULATION WITH LH Barcelos, A.C.Z.; Satrapa, R.A.;Nogueira, M.F.G.; Barros, C.M. .......................................................................................................... 513

FOLLTROPIN–V® DOSE TRIAL ON SUPEROVULATION PROTOCOL IN EUROPEAN BEEFCOWS Fernandes, C.A.C.; Figueiredo, A.C.S.; Oliveira, E.R.; Vasconcelos, T.D.; Alves, B.F.L.;Gioso, M.M.; Viana, J.H.M................................................................................................................. 514

OVARIAN RESPONSE AND EMBRYO PRODUCTION OF COWS SUPEROVULATED WITHA SINGLE SUBCUTANEOUS DOSE OF PLUSET OR FOLLTROPIN Alvarez, R.H.;Pires, R.M.L.; Martinez, A.C. ............................................................................................................. 515

IMPROVEMENT IN EMBRYO RECOVERY USING DOUBLE UTERINE FLUSHING INCATTLE, CORROBORATING DATA FROM A PREVIOUS STUDY Mollo, M.R.; Ramos, A.F.;Pivato, I.; Guimarães Neto, A.G.; Franco, M.M.; Rumpf, R.; Sartori, R. ........................................... 516

FACTORS AFFECTING PREGNANCY RATES IN A FIXED-TIME EMBRYO TRANSFERPROGRAM Peres, L.C.; Pincinato, D.; Tríbulo, R.; Cutaia, L.; Bó, G.A. ......................................... 517

EVALUATION OF TRYPSIN AND ANTIBIOTIC TREATMENTS ON THE INACTIVATIONOF LEPTOSPIRA SPP IN EXPERIMENTALLY INFECTED BOVINE THAWEDBLASTOCYSTS D’Angelo, M.; Galuppo, A.G.; Picolomini, M.; Athayde, C.S.; Santos, M.;Pavão, D.L.; Genovez, M.E.; Castro, V.; Garcia, J.M. ....................................................................... 518


XXIX

SEX IDENTIFICATION OF IN VIVO PRODUCED BOVINE EMBRYOS USING PCR UNDERFIELD CONDITIONS Sousa, R.V.; Cardoso, C.R.S.; Butzke, G.; Franco, M.M.; Rumpf, R. .......... 519

EFFECTS OF FSH TREATMENT ONSET ON IN VIVO EMBRYO PRODUCTION INSUPERSTIMULATED NELORE (Bos indicus) COWS Moino, L.L.; Porto, L.P.C.; Moraes, F.L.Z.;Carvalho, L.M.; Neto, J.D.S.; Spegiorin, M.R.; Silva, K.C.; David, F.L. .......................................... 520

EFFECTS OF SYNCHRONIZATION OF OVULATION ON IN VIVO EMBRYO PRODUCTIONIN SUPERSTIMULATED NELORE (Bos indicus) COWS Moino, L.L.; Porto, L.P.C.;Moraes, F.L.Z.; Carvalho, L.M.; Neto, J.D.S.; Spegiorin, M.R.; David, F.L. .................................... 521

STUDY OF FACTORS THAT INFLUENCE THE RESULTS IN A GOAT EMBRYO TRANSFERPROGRAM – DONOR Portela, A.P.M.; Ribeiro Filho, A. de L.; Chalhoub, M.; Alves, S.G.G.;Silva, A.A.B.; Santana, R.C.M.; Gusmão, A.L. .................................................................................. 522

FACTORS THAT INFLUENCE THE RESULTS IN A GOAT EMBRYO TRANSFER PROGRAM– RECIPIENTS Ribeiro Filho, A. de L.; Portela, A.P.M.; Chalhoub, M.; Silva, A.A.B.;Santana, R.C.M.; Freitas, D.S.; Gusmão, A.L. ................................................................................... 523

EFFECT OF NUMBER OF INSEMINATIONS ON THE EMBRYO PRODUCTION INSUPEROVULATED AND FIXED TIME ARTIFICIALLY INSEMINATED NELORE COWS(Bos indicus) Martins, C.M.; Sá Filho, M.F.; Souza, A.H.; Baruselli, P.S. ........................................ 524

EFFECT OF PROGESTERONE INJECTION AT THE BEGGINING OF THESUPEROVULATION PROTOCOL WITH FIXED TIME ARTIFICIAL INSEMINATION INNELORE (Bos indicus) DONORS Martins, C.M.; Torres-Júnior, J.R.S.; Souza, A.H.;Baruselli, P.S. ...................................................................................................................................... 525

SUPEROVULATION WITH eCG AND FSH ASSOCIATED WITH TIMED ARTIFICIALINSEMINATION IN NELORE DONORS (Bos indicus) Martins, C.M.; Torres-Júnior, J.R.S.;Gimenes, L.U.; Souza, A.H.; Baruselli, P.S. ....................................................................................... 526

SUPEROVULATORY RESPONSE AND EMBRYO PRODUCTION IN NELORE DONORS(Bos indicus) INDUCED TO OVULATE WITH GNRH OR LH FOR FIXED TIME ARTIFICIALINSEMINATION Martins, C.M.; Torres-Júnior, J.R.S.; Souza, A.H.; Baruselli, P.S. ....................... 527

EFFECT OF THE INTERVAL PROSTAGLANDIN TO LH ADMINISTRATION (48 OR 60HOURS) ON SUPEROVULATORY RESPONSE AND EMBRYO PRODUCTION IN NELORECOWS (Bos indicus) Martins, C.M.; Torres-Júnior, J.R.S.; Souza, A.H.; Baruselli, P.S. ................. 528

OVINE FSH DOSE ADJUSTMENT IN PROTOCOL FOR SUPEROVULATION ASSOCIATEDWITH FIXED TIME ARTIFICIAL INSEMINATION IN NELORE DONORS (Bos indicus)Martins, C.M.; Torres-Júnior, J.R.S.; Souza, A.H.; Baruselli, P.S. ..................................................... 529

EFFECT OF DIFFERING MOMENTS OF LH TREATMENT IN SUPERSTIMULATIONPROTOCOLS WITH FIXED TIME ARTIFICIAL INSEMINATION IN CROSSBRED COWS(HOLSTEIN X GIR) Silva, H.S.; Martins, C.M.; Ayres, H.; Torres-Júnior, J.R.S.; Souza, A.H.;Baruselli, P.S. ...................................................................................................................................... 530

EFFECT OF FREQUENCY IN LOW DOSE EQUINE PITUITARY EXTRACT TREATMENTIN INDUCTION OF MULTIPLE OVULATIONS IN MARES Alonso, M.A.; Fleury, P.D.C.;Alvarenga, M.A. .................................................................................................................................. 531


XXX

PREGNANCY RATES IN EMBRYO RECIPIENTS AFTER SUPPLEMENTATION WITHGNRH E HCG Machado, R.; da Silva, J.C.B.; Barbosa, R.T.; Niemeyer, C.; Binelli, M. ................ 532

PROTOCOL FOR IVF EMBRYO TRANSFER Dias, C.C.; Alvin, M.T.T.; Saliba, W.P.;Vasconcelos, J.L.M. ............................................................................................................................ 533

Abstracts - Nuclear Transfer and Transgenesis

EXPRESSION OF H19, IGF2 AND IGF2r IMPRINTED GENES IN CLONES PLACENTAYamazaki, W.; Perecin, F.; Braga, F.C.; Méo, S.C.; Biase, F.H.; Tarouco, A.K.; Ferreira C.R.;Merighe, G.K.F.; Meirelles, F.V.; Garcia, J.M. ................................................................................... 537

EFFECT OF EXOGENOUS DNA AND INCUBATION PERIOD IN THE INDUCTION OFAPOPTOSIS AND NECROSIS IN BOVINE SPERMATOZOA Feitosa, W.B.; Rocha, A.M.;Mendes, C.M.; Goissis, M.D.; Nicacio, A.C.; Gonçalves, J.S.A.; Visintin, J.A.;Assumpção, M.E.O.A. ......................................................................................................................... 538

EFFECT OF INCUBATION PERIOD ON TRANSFECTION RATES IN BOVINESPERMATOZOA Feitosa, W.B.; Avanzo, J.L.; Mendes, C.M.; Goissis, M.D.; Nicacio, A.C.;Delboni, C.C.; Visintin, J.A.; Assumpção, M.E.O.A. ......................................................................... 539

USE OF SPERMATOZOA AS VECTORS OF EXOGENOUS DNA FOR IN VITROPRODUCTION OF BOVINE TRANSGENIC EMBRYOS Simões, R.; Binelli, M.; Milazzotto,M.P.; Feitosa, W.B.; Nicacio, A.C.; Mendes, C.M.; Visintin, J.A.; Assumpção, M.E.O.A ............... 540

USE OF LENTIVIRAL VECTOR CARRYING SHRNA FOR MYOSTATIN KNOCKDOWNIN THE GENERATION OF TRANSGENIC NELORE EMBRYO Milazzotto, M.P.; Feitosa, W.B.;Strauss, B.E.; Bajgelman, M.; Simões, R. 1; Martins, L.F.; Assumpção, M.E.O.A.;Visintin, J.A. ........................................................................................................................................ 541

MOLECULAR AND MORPHOLOGICAL CHARACTERIZATION OF BOVINE FETALFIBROBLASTS EXPRESSING THE GREEN FLUORESCENT PROTEIN (GFP) INDIFFERENT PASSAGES Bressan, F.F.; Braga, F.C.; De Bem, T.H.C.; Merigue, G.K.;Miranda, M.; Mesquita, L.G.; Meirelles, F.V. ..................................................................................... 542

ACROSOMAL REACTION ON BOVINE SPERMATOZOA INCUBATED WITH ORWITHOUT EXOGENOUS DNA Feitosa, W.B.; Martins, L.F.; Rovegno, M.; Simões, R.;Delboni, C.C.; Marques, M.G.; Visintin, J.A.; Assumpção, M.E.O.A. .............................................. 543

INTEGRITY OF BOVINE SPERMATOZOA MEMBRANE INCUBATED WITH OR WITHOUTEXOGENOUS DNA Feitosa, W.B.; Rovegno, M.; Milazzotto, M.P.; Simões, R.; Gonçalves, J.S.A.;Nicacio, A.C.; Visintin, J.A.; Assumpção, M.E.O.A. ......................................................................... 544

QUALITATIVE MRNA EXPRESSION OF FGF8 IN CLONED AND NATURAL MATINGBOVINE PLACENTA Artoni, L.P.; Garbelotti, F.; Castilho, A.C.; Machado, M.F.; Price, C.;Papa, P.C.; Buratini, Jr., J. ................................................................................................................... 545

USE OF LENTIVIRUS IN THE PRODUCTION OF BOVINE FETAL FIBROBLASTSEXPRESSING THE GREEN FLUORESCENT PROTEIN (GFP) GENE Bressan, F.F.;Bajgelman, M.C.; Krieger, J.E.; Strauss, B.; Meirelles, F.V. .............................................................. 546


XXXI

EFFECT OF EXOGENOUS DNA ADDITION AND INCUBATION PERIOD ON BOVINESPERM MOTILITY Feitosa, W.B.; Milazzotto, M.P.; Simões, R.; Goissis, M.D.; Peres, M.A.;Golçalves, J.S.A.; Visintin, J.A.; Assumpção, M.E.O.A. ................................................................... 547

MOLECULAR AND MORPHOLOGICAL CHARACTERIZATION OF BOVINE FETALFIBROBLASTS EXPRESSING THE GREEN FLUORESCENT PROTEIN (GFP) INDIFFERENT PASSAGES Bressan, F.F.; Braga, F.C.; De Bem, T.H.C.; Merigue, G.K.;Miranda, M.; Mesquita, L.G.; Meirelles, F.V. ..................................................................................... 548

NUCLEAR TRANSFER IN SWINE: EVALUATION OF IN VITRO MATURATION OFOOCYTES FOR PRODUCTION OF RECIPIENT CITOPLASM Gerger, R.P.C.; Marques M.G.;Nascimento, A.B.; Mendes, C.M.; Nicacio, A.C.; Brandão, A.C.; Assumpção, M.E.O.A.;Visintin, J.A. ........................................................................................................................................ 549

RELATIONSHIP BETWEEN OCT-4 GENE EXPRESSION AND BOVINE EMBRYOPRODUCED BY NUCLEAR TRANSFER Camargo, L.S.A.; Viana, J.H.M.; Ramos, A.A.;Sá, W.F.; Ferreira, A.M........................................................................................................................ 550

Abstracts - Others

SEX RATIO OF IN VITRO FERTILIZED BOVINE EMBRYOS USING “X” SPERM SEXEDBY DENSITY GRADIENT CENTRIFUGATION: PRELIMINARY RESULTS Resende, M.V.;Perini, A.P.; Lucio, A.C.; Tetzner, T.A.D.; Vantini, R.; Hossepian de Lima, V.F.M. ......................... 553

LACK OF GENOMIC IMPRINTING OF IGF2 AND XIST IN PARTHENOTE BOVINE FETUSRECOVERED AT DAY 35 OF PREGNANCY Méo, S.C.; Ferreira, C.R.; Perecin, F.; Yamazaki,W.; Landim Junior, L.P.; Saraiva, N.Z.; Tetzner, T.A.D.; Leal, C.L.V.; Meirelles, F.V.;Garcia, J.M. ......................................................................................................................................... 554

CELLULAR CYCLE AND VIABILITY OF EQUINE FIBROBLASTS CULTURED UNTILCONFLUENCE PRE AND POST FREEZING Lima-Neto, J.F.; Fernandes, C.B.; Dores, C.B.;Golim, M.A.; Silva, V.A.; Landim-Alvarenga, F.C. ........................................................................... 555

GESTACIONAL AGE’S AND WEIGHT’S OF NEW BORN OVINE EVALUATIONTHROUGHOUT THE MEASURES GOTTEN FROM GESTATION’S DIFFERENTS PERIODSBY ULTRASONOGRAPHY Chiamenti, A.; Santos, M.H.B.; Moraes, E.P.B.X.; Chaves, R.M.;Gonzalez, C.I.M.; Torreão, J.N.C.; Reichenbach, H.-D.; Lima, P.F.; Oliveira, M.A.L. ..................... 556

ULTRASONOGRAPHIC EVALUATION OF EMBRYO-FETAL DEVELOPMENT IN EWESOF SAINT INES BREED. Moraes, E.P.B.X.; Santos, M.H.B.; Firmino, A.P.; Messias, J.B.;Caraciolo, M.C.M.; Lima, P.F.; Oliveira, M.A.L. .............................................................................. 557

DETERMINATION OF THE GENITAL TUBERCLE MIGRATION PERIOD TO EARLYSEXING SHEEP FETUSES OF DAMARA, SAINT INES AND 3/4 CROSSBRED DAMARA-SAINT INES. Santos, M.H.B.; Moraes, E.P.B.X.; Chiamenti, A., Rocha, J.M.; Paula, N.R.O.;Silva, G.A.; Lima, P.F., 4; Oliveira, M.A.L. ........................................................................................ 558

SEXING OF FETUSES BOER GOAT BREED BY ULTRASONOGRAPHY Santos, M.H.B.;Chiamenti, A.; Moraes, E.P.B.X.; Loureiro, B.; Soares, A.T.; Reichenbach, H.-D.; Lima, P.F.;Oliveira, M.A.L. .................................................................................................................................. 559


XXXII

SEXING OF DORPER SHEEP FETUSES DERIVED FROM NATURAL MATING ANDEMBRYO TRANSFER BY ULTRASONOGRAPHY Santos, M.H.B.; Moraes, E.P.B.X.;Gonzalez, C.I.M.; Gondim, F.Q.B.; Guido, S.I.; Reichenbach, H.-D.; Lima, P.F.;Oliveira, M.A.L. ................................................................................................................................. 560

EFFECT OF DIFERENT CONCENTRATIONS OF INDOL ACETIC ACID IN THEACTIVATION AND GROWTH OF NELORE PREANTRAL FOLLICLES. Blaschi, W.; Andrade,E.R.; Delboni, M.I.; Padilha, L.C.; Oliveira, A.C.S.; Alfieri, A.A.; Figueiredo, J.R.;Seneda, M.M. ...................................................................................................................................... 561

SWIN-UP INFLUENCE ON BOVINE EMBRYOS IN VITRO PRODUCTION USING “X”SPERM SEXED BY DENSITY GRADIENT CENTRIFUGATION: PRELIMINARY RESULTSLucio, A.C.; Fazano, F.A.T.; Perini, A.P.; Resende, M.V.; Tetzner, T.A.D.; Vantini, R.;Hossepian de Lima, V.F.M. ................................................................................................................. 562

COMPARISON BETWEEN TWO EXTENDERS TO COOL EQUINE SEMEN FOR 24 HOURSAT 5ºC BEFORE FREEZING Papa, F.O.; Melo, C.M.; Alvarenga, M.A.; Trinque, C.L.A.;Dell’Aqua Jr., J.A.; Felício, G.B.; Villaverde, I.A.B. ......................................................................... 563

THE USE OF DONKEY FROZEN SEMEN Oliveira, J.V.; Alvarenga, M.A.; Melo, C.M.;Macedo, L.M.; Dell’Aqua Jr., J.A.; Papa, F.O. ................................................................................... 564

COMPARISON BETWEEN TWO SHIPMENT CONTAINERS ON VIABILITY OF EQUINESEMEN COOLED FOR 24H BEFORE FREEZING Melo, C.M.; Alvarenga, M.A.; Zahn, F.S.;Martin, I.; Felício, G.B.; Dell’Aqua Jr., J.A.; Papa, F.O. .................................................................... 565

THE USE OF DIFFERENT EXTENDERS FOR CENTRIFUGATION ANDCRYOPROTECTANTS ON EQUINE SEMEN FERTILITY Melo, C.M.; Alvarenga, M.A.;Dell’Aqua Jr., J.A.; Felício, G.B.; Villaverde, I.A.B.; Papa, F.O. ....................................................... 566

THE USE OF DIFERENT EXTENDERS ON COOLING EQUINE SEMEN AT DIFFERENTTEMPERATURES Farrás, M.C.; Avanzi, B.R.; Melo, C.M.; Alvarenga, M.A.; Dell’Aqua Jr, J.A.;Medeiros, A.S.L.; Araújo, G.H.M.; Papa, F.O. .................................................................................... 567

GESTACIONAL AGE’S AND WEIGHT’S OF NEW BORN CAPRINE EVALUATIONTHROUGHOUT THE MEASURES GOTTEN FROM GESTATION’S DIFFERENTS PERIODSBY ULTRASONOGRAPHY Chiamenti, A.; Santos, M.H.B.; Moraes, E.P.B.X.; Chaves, R.M.;Soares, A.T.; Silva, G.A.; Reichenbach, H.-D.; Lima, P.F.; Oliveira, M.A.L. ................................... 568

EVALUATION OF MORFOLOGY FROM FEMALE BUFFALO OOCYTES EXPERIMENTALYINFECTED BY BoHV-1 D’Angelo, M.; Galuppo, A.G.; Souza, R.J.; Picolomini, M.; Santos, M.;Pavão, D.L.; Athayde, C.S.; Fontenele, A.D. ...................................................................................... 569

COULD PARTHENOGENETIC EMBRYOS ASSIST IN MATERNAL RECOGNIZANCE OFBOVINE PREGNANCY? (PARTIAL RESULTS) Almeida, A.B.; Madureira, E.H.;Merighe, G.K.F.; Braga, F.C.; Marques, V.B.; Pimentel, J.R.V.; Meirelles, F.V. .............................. 570

EFFECT OF DIFFERENT COOLING RATES AND DILUENTS ON GOAT SEMENPRESERVATION Bispo, C.A.S.; Palhão, M.P.; Fürst, R.; Rovay, H.; Carvalho, G.R.;Bispo, M.S. .......................................................................................................................................... 571

FIBROBLAST GROWTH FACTOR 13 (FGF-13) GENE EXPRESSION IN BOVINE ANTRALFOLLICLES Costa, I.B.; Castilho, A.C.S.; Machado, M.F.; Teixeira, N.A.; Price, C.A.;Buratini Jr, J. ....................................................................................................................................... 572


XXXIII

DETERMINATION OF THE GENITAL TUBERCLE MIGRATION IN MORADA NOVA SHEEPFETUSES BY ULTRASONOGRAPHY Santos, M.H.B.; Rabelo, M.C.; Guido, S.I.;Torreão, J.N.C.; Lopes Júnior, E.S.; Freitas, V.J.F.; Lima, P.F.; Oliveira, M.A.L. ............................. 573

PREGNANCY DIAGNOSIS IN SANTA INÊS EWES BY TRANSRECTAL ANDTRANSVAGINAL ULTRASONOGRAPHY Moraes, E.P.B.X.; Santos, M.H.B.; Aguiar Filho,C.R.; Firmino, A.P.; Paula, N.R.O.; Lima, P.F.; Oliveira, M.A.L. ...................................................... 574

IN VITRO CULTURE OF CAPRINE PREANTRAL FOLLICLES USING ANTIOXIDANTSUBSTANCES (α-TOCOPHEROL AND TERNATIN) Lima-Verde, I.B.; Bruno, J.B.; Martins,F.S.; Matos, M.H.T.; Maia Jr., J.E.; Silva-Filho, F.J.M.; Báo, S.N.; Silveira, E.; Santos, R.R.;Silva, J.R.V.; Lima, P.F.; Figueiredo, J.R.; Rodrigues, A.P.R. ............................................................ 575

ULTRASTRUCTURAL ANALYSIS OF EQUINE CUMULUS-OOCYTES COMPLEXES Boff,A.L.N.; Curcio, B.R.; Lins, L.; Velho, J.; Larvada, A.; Albuquerque, L.P.; Nogueira, C.E.W.;Deschamps, J.C. .................................................................................................................................. 576

QUANTIFICATION AND MORPHOMETRY OF PREANTRAL FOLLICLES OF BUFFALOESFETUSES Santos, S. S.D.; Ferreira, M.A.P.; Silva, M.S.; Cordeiro, M.S.; Biondi, F.C.;Ohashi, O.M. ....................................................................................................................................... 577

DEFINITION OF MINIMUM REPRESENTATIVE AREA FOR SONOGRAPHIC BOVINECORPUS LUTEUM IMAGE ANALYSIS Viana, J.H.M.; Siqueira, L.G.B.; Diniz, E.S.; Camargo,L.S.; Oliveira, E.R.; Fonseca, J.F.; Fernandes, C.A.C. ....................................................................... 578

MORTALITY DETERMINATION AND GENDER IDENTIFICATION OF CONCEPTUSES INPREGNANT SANTA INES EWES BY ULTRASONOGRAPHY Moraes, E.P.B.X.; Santos,M.H.B.; Chiamenti, A.; Rocha, J.M.; Falcão, D.P.; Albuquerque Filho, P.P.; Firmino, A.P.;Lima, P.F.; Oliveira, M.A.L. ................................................................................................................ 579

EVALUATION OF TWO METHODS OF STAINING FOR ASSESSMENT OF MORPHOLOGYAND ACROSOME IN DOMESTIC CAT (Felis catus) SPERMATOZOA Villaverde, A.I.S.B.;Melo, C.M.; Corrente, J.E.; Papa, F.O.; Lopes, M.D. ......................................................................... 580

ULTRASTRUCTURAL ANALYSIS OF OVINE FROZEN-THAWED PREANTRALFOLLICLES Tsuribe, P.M.; Landim-Alvarenga, F.C. ........................................................................ 581

VIABILITY OF EQUINE FIBROBLASTS AFTER CELLULAR CYCLE SYNCRONIZATIONUSING TWO METHODS Lima-Neto, J.F.; Fernandes, C.B.; Dores, C.B.; Golim, M.A.;Silva, V.A.; Landim-Alvarenga, F.C. .................................................................................................. 582

MORPHOLOGIC ALTERATIONS IN DOGS AND CATS GENITAL ORGANS FROM VILASRURAIS OF THE UMUARAMA REGION, PARANÁ STATE* Previato, P.F.G.; Pinto-Neto, A.;Werner, P.R.; Acco, A.; Mota, M.F.; Silva, A.V.; Fonseca, J.F. .......................................................... 583

ARE SUPEROVULATION PROTOCOLS REALY EFFICIENT FOR EMBRYO PRODUCTIONIN INBRED MICE LINEAGES? Wohlres, S.; Pinto, I.S.B.; Batista, R.I.T.; Faza, A.P.; Paiva, F.P.;Maffili, V.V.; Camargo, L.S.; Viana, J.H.M. ....................................................................................... 584

LOOKING FOR THE VITELLINE PLACENTATION IN EARLY BOVINES EMBRYOSMançanares, C.A.F.; Carvalho, A.F.; Santos, J.M.; Alberto M.L.V.; Assis Neto, A.C.; Ambrósio,C.E.; Martins, D.S.; Toquetti, R.C.; Miglino, M.A. ........................................................................... 585


XXXIV

CARUNCULAR FUSION IN PREGNANT COWS Pereira, F.T.V.; Barreto, R.S.N.;Miglino, M.A.; Silva, S.M.; Burioli, K.C. .......................................................................................... 586

USE OF DIFFERENT eCG DOSES FOR ESTROUS INDUCTION IN HAIR SHEEP INREPRODUCTION OUTSEASON IN THE NORTH OF PARANÁ Sartori Jr., G.; Melo Sterza,F.A.; da Cunha Filho, L.F.C.; Rego Grecco, F.C.A.; Santos, E.S.; Fonseca, A.; Baudraz, J.A. ......... 587

SEX DETERMINATION IN BOVINE USING AMELOGENIN GENE AMPLIFICATION:PROTOCOL OPTIMIZATION Leal, S.R.; Fontes, R.S.; Souza Filho, G.A.; Marques, V.C.L.;Matos, L.F. ........................................................................................................................................... 588

AUTHOR INDEX ............................................................................................................................... 589


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PRE-CONFERENCESHORT COURSES


XXXVI

acta scientiae veterinariae 34(supplement 1): 2006

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