fusion cell gene expression profile
Post on 23-Jan-2016
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•Fusion cell gene expression profile
•Molecular mechanism of target gene regulation by Dys::Tgo
•Identify enhancer of dys targets: CG13196, CG15252 and mbo
•Identify dys fusion cell enhancer
dys-gal4::UAS-CD8-GFP btl-gal4::UAS-CD8-GFP
dys-gal4::UAS-CD8-GFP dys-gal4::UAS-CD8-GFP ; dys/dys
•Identify dys target gene
•Co-activator or repressor of Dys::Tgo?
LP05454
Exon1 2 3 4 5 6 7 8 9 10
dys 2 (1.7kb)
dys1 (2.7kb)
dys un (3.8kb)
dys intron 2a (3.8kb)
dys intron2b (3.8kb)
Identify dys enhancer
dys1a (1.7kb)
dys1b (1.0kb)
dys intron1 (1.4kb)
dys1b-A (500bp) dys1b-B (500bp)
Enhancer for brain, hindgut and anal pad
dys intron-1 dys intron-2b
dys intron-2a
dys1 fragment contains dys fusion cell enhancer
dys un (S15-17)
dys1 (S12-17)
dys2 ( S15-17)
DT
DT
LT
DB
Leading edge?
DB
DT
LT
S14
S17S16
S14
dys1b (S12-17)
S14
S16
dys1a (S14-17)
S15
S14
S12 S12
dys target gene: CG15252
Exon1
CG15252 con (3.2kb)
9.0 kB 5’ upstream
CG15252 TCGTG (0.8 kb)
TCGTG TCGTG TCGTG
CG15252 enhancer
CG15252 con
CG15252 TCGTGDT
S16
CG15252 enhancer
TCGTG-a 185 bp
TCGTG-b 360 ssbp
TCGTG a+b (270bp) TCGTG c+d (100bp)
TCGTG-c 774 bp
TCGTG-d 792 bp
818bp
TCGTG to GATCCmutation: (waiting for result)
( NO?) (Yes!)
CG15252 enhancer: 100bp with 2 TCGTG sites
DT
DT
S16
S17
CG13196
500bp
350bp
100bp?
CG13196-500 CG13196-350
490bp
Ungoing.....
CG15252 DT expression depends on Sal?
•CG15252-GFP expression in Sal mutant?•Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-sal; UAS-dys•Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-kni; UAS-dys
Co-activator or repressor of Dys::Tgo?
•Compare enhancer of CG13196 and CG15252, identify potential binding sites (drifter?)
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