live food culture

Speaker: Dr. Malobika Das Trakroo

Live Food (Living nutritious Live Food (Living nutritious Capsules)Capsules)

• Cell SizeCell Size

• Bio-chemical CompositionBio-chemical Composition

• Toxic CompoundsToxic Compounds

• DigestibilityDigestibility

Organisms Tubifex

Chironomous larvae

Infusoria

Zooplankton

Micro algae

Application

Hatchery Operation

Aquarium Industry

Water Renovation

Closed Life Support System

Single Cell Production(SCP)

Micro algal cultureBivalve Molluscs (Clams, Mussels, Oysters, Scallops, throughout life)

Young stages of Shrimps

First feeding stage of some fishes

Small invertebrates i.e. Rotifers and Artemia

Live Feed For

Aspects of Micro Algal Aspects of Micro Algal CultureCulture

• IsolationIsolation

• Culture Media Selection & PreparationCulture Media Selection & Preparation

• Stock Culture MaintenanceStock Culture Maintenance

• Mass CultureMass Culture

• HarvestHarvest

Isolation

Methods

Pipette

Centrifuge

Agar Plating

Exploiting Phototactic Movement

Serial Dilution Culture Technique

Culture MediaCulture MediaFew Common Media:Few Common Media:

• BBM (Bold’s Basal Medium)BBM (Bold’s Basal Medium) For Green For Green algaealgae

• Conway or Walne’s MediumConway or Walne’s Medium For Micro algae For Micro algae ( Phytoflagellate)( Phytoflagellate)

• Guillard’s F/2 MediumGuillard’s F/2 Medium For MicroalgaeFor Microalgae

• TMRL MediumTMRL Medium For Diatoms For Diatoms

• SD IISD II For Green algaeFor Green algae

• DY IIIDY III For Flagellates For Flagellates (Crysophytes & Cryptomonas)(Crysophytes & Cryptomonas)

• BG IIBG II For Blue green For Blue green algaealgae

Growth Phase of Algal

Phase Declining

Stationary

Death

Lag

Exponential

Stock Culture MaintenanceStock Culture Maintenance

Stock culture room is a small AC room adjacent Stock culture room is a small AC room adjacent to mass culture room.to mass culture room.

Material Required:Material Required:– Haufkin culture flaskHaufkin culture flask– Nutrient mediaNutrient media– InoculumInoculum– IlluminationIllumination– Controlled temperatureControlled temperature

Strict anti contamination procedure has to be Strict anti contamination procedure has to be followed.followed.

Mass CultureAC room with light arrangement and centralized

aeration system.

1.ContainersGlass carbuoys (20 l)

Polythene bags (10 l)

Perspex tanks (100 l)

Fiber glass tanks (250 l)

2. Inoculum Size 250 ml for Glass carbuoys

100 ml for Polythene bags

2 l for Perspex tanks

3. Illumination3. Illumination

4. Temperature control4. Temperature control

5. Aeration5. Aeration

6. Glass wares6. Glass wares

Harvest and Preservation

Wet

Dry

FlocculationHarvesting

Preservation

Rotifer Culture

Brachionus Culture System

Raising Brachionus Culture

Raising appropriate food organism

Mass Production

Harvesting

Raising Food Organism

ChlorellaCulture

Continuous Culture

Feed back Culture

Daily Tank Transfer Method

CIFE Method

Mass Production

Batch Culture

FeedbackCulture

Semi continuous Culture

Out Door Culture Method

Slurry Tank(300 l)

10 kg chicken dropping+

5 kg GNOC+

2.5 kg SSP

FilteredWater(250 l) Aeration

Mass Culture Tank(300 l)

5th day

7th day

10th day

Rotifer100-150 N/ml

Moina20-25 N/ml

Daphnia10-20 N/ml

InoculationRotifer

( 30-40 N/ml)

Moina(10-15 N/ml)

Daphnia(10 N/ml)

3rd day

3 ml/l from 4th day4 ml/l for 3 days

Moina CultureMoina Culture

Stock RaisingStock Raising

Mass CultureMass Culture

HarvestingHarvesting

Mass Culture

Outdoor Culture (CIFE)

Application of ManurePoultry Manure4 gm/l weekly

Cow dung Substrate10 gm/l

Microbial Water100 gm PM/l

Anaerobically treated

Filtered extract 10-20l/1000l

Anaerobically digested

HarvestingHarvesting

Non selectiveNon selective

SelectiveSelective

AlternateAlternate

Infusoria Culture

By

Using Banana peelings

Using Hay infusion

Using Apple snail