vascular injury panel 2 (human) kits
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Table of Contents
Contact Information MSD Customer Service
MSD Scientific Support
Introduction
µ
Principle of the Assay
Kit Components
Additional Materials and Equipment
Optional Materials and Equipment
Safety
Best Practices and Technical Hints
Protocol
Validation
Analysis of Results
Typical Data
Vascular Injury Panel 2 (human)
1 10 100 1000 10000 100000 1000000
100
1000
10000
100000
1000000
10000000
SAA
CRP
VCAM-1
ICAM-1
Concentration (pg/mL)
Sig
nal
Precision
Dilution Linearity
% 𝑅𝑒𝑐𝑜𝑣𝑒𝑟𝑦 =𝑚𝑒𝑎𝑠𝑢𝑟𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛
𝑒𝑥𝑝𝑒𝑐𝑡𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛× 100
Spike Recovery
% 𝑅𝑒𝑐𝑜𝑣𝑒𝑟𝑦 =𝑚𝑒𝑎𝑠𝑢𝑟𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛
𝑒𝑥𝑝𝑒𝑐𝑡𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛× 100
Specificity
% 𝑁𝑜𝑛𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐𝑖𝑡𝑦 =𝑛𝑜𝑛𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐 𝑠𝑖𝑔𝑛𝑎𝑙
𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐 𝑠𝑖𝑔𝑛𝑎𝑙× 100
Interference
Stability
Calibration
Tested Samples
Assay Components
References
Appendix A
10 100 1000 10000 100000 1000000100
1000
10000
100000
1000000
10000000
Wash
Remove Wash
SAA Concentration (pg/mL)
Sig
nal
1 10 100 1000 10000 100000100
1000
10000
100000
1000000
10000000
Wash
Remove Wash
CRP Concentration (pg/mL)
Sig
nal
10 100 1000 10000 100000 100000010
100
1000
10000
100000
1000000
10000000
Wash
Remove Wash
VCAM-1 Concentration (pg/mL)
Sig
nal
1 10 100 1000 10000 10000010
100
1000
10000
100000
1000000
10000000
Wash
Remove Wash
ICAM-1 Concentration (pg/mL)
Sig
nal
Appendix B
10 100 1000 10000 100000 100000010
100
1000
10000
100000
1000000
10000000
Multiplex
Individual Assay
SAA Concentration (pg/mL)
Sig
nal
1 10 100 1000 10000 10000010
100
1000
10000
100000
1000000
10000000
Multiplex
Individual Assay
CRP Concentration (pg/mL)S
ignal
10 100 1000 10000 100000 100000010
100
1000
10000
100000
1000000
10000000
Multiplex
Individual Assay
VCAM-1 Concentration (pg/mL)
Sig
nal
1 10 100 1000 10000 10000010
100
1000
10000
100000
1000000
10000000
Multiplex
Individual Assay
ICAM-1 Concentration (pg/mL)
Sig
nal
Summary Protocol
o
o
Catalog Numbers
Plate Diagram
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