Anti-Importin 3

NIH

3T3

cells

Prim

ary

hipp

ocam

pal n

euro

ns

Mou

se E

S c

ells

Anti-Importin 1 Anti-Importin 1

NIH

3T3

cells

Prim

ary

hipp

ocam

pal n

euro

ns

Mou

se E

S c

ells

NIH

3T3

cells

Prim

ary

hipp

ocam

pal n

euro

ns

Mou

se E

S c

ells

Anti-E47

NIH

3T3

cells

Prim

ary

hipp

ocam

pal n

euro

ns

Mou

se E

S c

ells

A B

C

D

Supplementary figure 1

NIH

3T3

cells

Prim

ary

hipp

ocam

pal n

euro

ns

Mou

se E

S c

ells

E

Anti-NeuroD1

* *

*

**

Anti-GAPDH

NIH

3T3

cells

Prim

ary

hipp

ocam

pal n

euro

ns

Mou

se E

S c

ells

F

Flag-NeuroD1

Flag-134-161

Anti-flag DAPI

Supplementary figure 2

GFP Injection Marker

NeuroD1-GFP

NeuroD1-GFP + RanQ69L-GTP

HeLa

A B

C D

Supplementary figure 3

NIH3T3

E F

HG

GFP Injection Marker

Supplementary figure 4

Anti-RanBP5 Anti-RanBP7

Neu

roD

1-G

ST

+cyt

osol

Neu

roD

1-G

ST

+cyt

osol

+R

anQ

69L

－

GTPHel

a ce

ll ly

sate

GS

T be

ads+

Cyt

osol

Neu

roD

1-G

ST

+cyt

osol

Neu

roD

1-G

ST

+cyt

osol

+R

anQ

69L

－

GTPHel

a ce

ll ly

sate

GS

T be

ads+

Cyt

osol

75100

150

A B

Supplementary figure 5

Importin 1 Importin + 1

(NeuroD1)

(A)

Database : NCBInr 20100904 (11744690 sequences; 4010973687 residues)Taxonomy : Homo sapiens (human) (232987 sequences)Timestamp : 8 Sep 2010 at 09:45:05 GMTTop Score : 182 for gi|19923142, importin subunit beta-1 [Homo sapiens]

Mascot Score Histogram

Protein score is -10*Log(P), where P is the probability that the observed match is a random event.Protein scores greater than 66 are significant (p<0.05)

Search Parameters

Enzyme : TrypsinFixed modifications : Carbamidomethyl (C)Variable modifications : Oxidation (M)Mass values : MonoisotopicProtein Mass : 95 kDaPeptide Mass Tolerance : ± 0.2 DaPeptide Charge State : 1+Max Missed Cleavages : 1Number of queries : 36

(B)

Supplementary figure 6