anti viral chemotherapy
TRANSCRIPT
ANTI VIRAL CHEMOTHERAPY
SARATH T M AMRITA UNIVERSITY
DNA / RNA virus
Attachment Entry ( endocytosis / envelope fusion)UncoatingGenome replicationTranscription & TranslationAssemblyMaturationEgress ( cell lysis/ budding)Release
HIVAttachmentEntryUncoatingReverse transcriptionIntegrationTranscription& translationAssemblyBuddingMaturation
DNA VIRUS
HIV
INHIBITORS OF VIRAL UNCOATING
UNCOATING OF INFLUENZA -A VIRUS
INHIBITORS OF VIRAL GENOME REPLICATION
----NUCLEOSIDE ANALOGUES
ACYCLOVIR (ACV)
Against Herpes simplex virus (HSV/ HHV 1&2) & Vericella zoster (VZV/ HHV-3 )Inhibitor of viral DNA polymeraseHigh therapeutic indexGuanine base attached to an incomplete sugar ringDrug has to be activated to its triphosphate form
ACV
Acyclovir viral
Thymidine kinaseAcyclovir monophosphate
Acyclovir diphosphate
Cellular kinase
Acyclovir triphosphatecellular
kinase
Incorporates into DNA instead of dGTP opposite to a C residue
(pppACV )
Chain termination as pppACV has no 3’OH
Enzyme forms dead-end complex with upcoming d-nucleoside triphosphate---inactive enzyme
viralDNA polymerase
Zidovudine (Azidothymidine) ( AZT )
Against HIVInhibitor of reverse transcriptaseThymidine base attached to a sugar in which 3’ OH is converted to an azido group
AZT AZT monophosphate
Cellular kinase
(HIV does not encode own kinase)
AZT diphosphate
Thymidylate kinase
AZT triphosphate
RT
Chain termination, inactive enzyme
cellular
No selectivity at activation step (hence toxicity is a serious issue)Potent inhibitor of HIV RT than human DNA polymerase
Cellular nucleoside
diphosphate kinase
NNRTIs (Non-nucleoside analog RT inhibitors)
As with the nucleoside analogs, the target enzyme is reverse transcriptase.
However, NNRTIs bind directly and non-competitively to the enzyme at a position in close proximity to the substrate binding site for nucleosides. ( called as NNRTI pockets)
The resulting complex blocks the active site of the reverse transcriptase.
This, in turn, can bind fewer nucleosides, slowing polymerization down significantly.
In contrast to NRTIs, NNRTIs do not require activation within the cell. Eg: efavirenz
INHIBITORS OF VIRAL MATURATION
RITONAVIRInhibitor of HIV proteaseIn the long protein chain , a sequence of Phe-Pro is a site of cleavage for HIV proteaseAn unusual site of cleavage for human proteaseRitonavir is an analogue of this sequence in which Pro is replaced by PheInstead of C = O of peptide CHOH was used to mimic transition stateProteins formed are not processed efficientlyVirions that bud from infected cells remains immature & non-infectious