bacillus. bacillus sp. gp rod spore former examples –b. anthracis –b. cereus –b. subtilis...
TRANSCRIPT
Bacillus
Bacillus sp.
• GP rod• Spore former• Examples
– B. anthracis– B. cereus– B. subtilis– B. megatarium
• Exoenzymes– Lipase– Amylase– Casease– Gelatinase
Spores
Agar Characteristics
Starch Hydrolysis
• Enzymes– Amylase– Maltase
• Substrate– Starch
• Reaction– Clear zone
Protein Hydrolysis
• Enzymes– Proteases– Caseases
• Substance– Casein
• Reaction– Clear zone
Lipid Hydrolysis
• Enzymes– Lipase
• Substrate– Tributyrin
• Reaction– Clear zone
Bacillus Tri-Plate
Collagen Hydrolysis
• Enzyme– Gelatinase
• Use loop (vs. needle)– Inoculate several times– Stab deep
• Substrate– Gelatin
• Reaction– Clear Zone on Agar– Liquefy Gelatin in tube
MSA• Selective
• Differential
• Substrate– Mannitol– Salt (7.5%)
• pH = phenol red
• Reaction– Growth– Fermentation
Nitrate Reduction
• Inoculate with loop• Incubate • Additional solutions
– Sulfanilic acid (A)– Naphthylamine (B)
• (+) = Red– Nitrate to Nitrite
• No color, add Zinc– (+) = not red– (-) = red
Citrate• Enzyme
– Citrase• Substrate
– Citrate• pH
– Bromthymol blue• Reaction
– Keto acids• Oxaloacetic
– Pyruvate
• Acetic acid– CO2
– CO2 + NaH2CO3– Prussian blue = +
Bile Esculin Agar (BEA)
• Selective– Bile Salts
• Differential– Esculin (CH2O)– Fe-NH3-citrate
• Reaction– Growth in bile– Hydrolyze esculin
• Esculetin-Iron Rxn• Black precipitate
Results
• Exoenzymes– Starch (amylase, maltase)– Lipid (lipase)– Proteins (Casease, protease)– Collagen (Gelatinase)
• Reaction– (+) = clearing– (-) = no clearing
Starch Hydrolysis
Lipid Hydrolysis
Protein Hydrolysis
Gelatin (Collagen) Hydrolysis
BEA and Citrate Results
Bile Esculin Citrate
+ +
Hydrolysis Results Summarycasein lipid
Gelatin Starch