bbi solutions - antibodies
TRANSCRIPT
Antibodies
“Our recombinant Fab
Antibodies can improve
assay sensitivity”
Be confident in your results when you
choose BBI’s monoclonal antibodies for
your next IVD assay:
Tested in ELISA
Tested in lateral flow
Recommended pairings provided
Strong customer feedback
NEW: Highly sensitive Recombinant
Fab antibodies to improve your next
generation assays
Custom development and
manufacturing services to meet your
individual requirements
Primary antibodies for diagnostic assays
Top mouse monoclonal antibodies
Lane 1: Native Human D-
Dimer
Lane 2: Human Serum
Lane 3: Native Fibrinogen
Lane 4: Native Plasminogen
Exclusive D-Dimer clones 1D2 and 3B6
Validated in Lateral Flow, Sandwich ELISA,
Western Blot and Latex Agglutination
Does not detect the closely related molecules
Fibrinogen and Plasminogen
Cited in 100’s of peer-reviewed journal
papers
Antibodies for cancer diagnosis
CA15-3 & CA19-9 antibody pairs
Matching pairs validated in sandwich ELISA
against real patient samples
Results correlate with commercially available tests
Top mouse monoclonal antibodies
HbA1c antibodies for Diabetes
Validated in Lateral Flow and Sandwich ELISA
Tested in patient samples
Results correlate with commercial assays
Infectious Disease antibodies
Tested in Lateral Flow
Tested using patient samples
Comprehensive range of biomarkers covered
NEW! Recombinant Fab antibodies
Exceptional
Sensitivity
The range of detection is determined
for all of our recommended pairs
Reliable
Production
Produced in E.coli to shorten lead
times and avoid scaling issues
Phage
Technology Phage technology screens 4500 pairs to
make sure the best pair is discovered
High
Affinities
Picomolar affinities ensure enhanced
antibody binding
Using Phage Display Technology
1m antibody clones generated, enhancing the
opportunity of finding the best antibodies
4500 antibody pairs are screened to ensure the
best pair is identified
Patient sample testing to identify the best pair
Immunisation
of mice
Transfer antibody
coding DNA to
phage
Antibodies selected
for antigen binding
Related antigens
added in excess to
screen out cross
reactions
Candidate genes
transferred to
plasmid and E.coli
Antibodies purified
Matched pair
screening - Luminex
Patient
sample
testing
The development process
Example: Calcitonin Recombinant Fab
antibody
Patients with calcitonin levels >100 pg/mL
have a high risk for medullary thyroid
carcinoma (~90%–100%) Toledo et al.,
(2009)
Our Calcitonin antibody pair detects at a
range of 6 to 2000 pg/mL
These highly sensitive
antibodies are ideal for
evaluating risk of this carcinoma
“Reduce your time and
money on reagents with
unproven performance – our
results speak for
themselves”
Demonstrated Clinical relevance
Recombinant Fab antibodies vs.
standard monoclonal antibodies
Standard Monoclonal Antibodies
Recombinant Fab Monoclonal
Antibodies
Initial Screen 100’s of clones screened 1,000,000,000 clones screened
Matched Pairing Best few antibodies tested for pairing >4500 antibody pairs tested (Luminex
platform)
Affinity micromolar as standard picomolar as standard
Sensitivity microgram/ml as standard picogram/ml as standard
Validation Purified Antigen Clinical samples
Conjugation May interfere with antigen binding
region
Engineered Cysteine residue ensures no
interference
Production
Cell culture - slow, expensive,
unreliable Ascites - slow, uses many
animals, illegal in EU
E. coli - fast, reliable, easy to scale-up,
simple storage
Antigen binding sites per
mg of purchased product Standard 36% more
Demonstrated exceptional performance
Our CRP Antibody has a significantly lower limit of detection than other high-
performance CRP antibodies on the market
Benefit: make assays which are more sensitive than those of your
competitors – increasing effective diagnosis and time to result on a like for
like basis
0
200
400
600
800
1000
1200
10
0
75
50
25
10 5 2 1 0
Peak h
eig
ht
CRP conc (ng/mL)
Comparison of BR228-D4A3 with commercially available antibodies
BR228-D4A3
Competitor A
Competitor B
Flu Fab
Reader
CRP conc (ng/ml) 100 75 50 25 10 5 2 1 0
BBI antibody, BR228-D4A3
Competitor B antibody
Competitor A antibody
Visual
Lateral Flow: Comparison of BR228-
D4A3 with commercially available
antibodies
Our Capabilities | Monoclonal Antibodies
Cardiac Infectious Disease Malaria HRP2 Veterinary
D-Dimer C. difficile Toxin A Malaria LHD (pan) Canine Distemper
Troponin I C. difficile Toxin B Malaria LDH (Pf & Pv) Virus
Chikungunya Norovirus Avian Influenza H5
Diabetes Dengue NS1 PBP2a FeLV
Hb Ebola Reston TB Brucella LPS
HbA1c Ebola Sudan Enrofloxacin
Ebola Zaire Tumour Goat IgG
Drugs of abuse H. pylori CA125 Canine IgM
THCΔ9 HAV CA15-3 Chicken IgY
Hepatitis B CA19-9 GST
Hormones HIV p24 Calcitonin
Progesterone HIV gp35
HIV gp41 Immunoglobulins
Pregnancy Influenza A IgA
AFP Influenza B IgE
Leptospira
Listeria
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Our Capabilities | Polyclonal Antibodies
Inflammation Pregnancy Renal
CRP AFP A2M
B2M
Immunoglobulins Inflammatory Cystatin C
IgA A1AT
IgM
IgG Haematology Metabolism
Albumin Haptoglobin Ceruloplasmin
Ferritin
Haemoglobin
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Our Capabilities | Recombinant Fab Antibodies
* Detection Ranges are assay dependent and optimised for specific
clinical conditions, therefore may be improved with optimisation.
Biomarker Detection Range*
Capture
Antibody Detection Antibody
Adiponectin 0.1 - 525 ng/mL BR101-P7E2 BR101-M3H8
Adiponectin 230 - 59118 ng/mL
BR101-H2B4 (Competitive ELISA)
Angiopoietin 2 0.001 - 97 ng/mL BR103-P4E8 BR103-V5F2
Big Endothelin 1 0.001 - 1.8 ng/mL BR104-D6A4 BR104-H1A4
Calcitonin 0.006 - 2 ng/mL BR388-G6B1 BR388-D5C4
Cathepsin D 0.03 - 150 ng/mL BR107-F5G2 BR107-K6D3
CKBB 0.15 - 33 ng/mL BR112-V6F6 BR112-Z1C4
CRP 151 - 231000 ng/mL
BR228-D4A3 (Competitive ELISA)
Cystatin C 165 - 9073 ng/mL
BR175-G8D9 (Competitive ELISA)
Cystatin C 165 - 9073 ng/mL
BR175-H4C8 (Competitive ELISA)
E. histolytica not tested BR116-K9F7 BR116-G3A1
Eotaxin 0.007 - 2 ng/mL BR108-M7H3 BR108-K2D9
E-selectin 0.05 - 235 ng/mL BR117-K2E7 BR117-H5B6
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Our Capabilities | Recombinant Fab Antibodies
* Detection Ranges are assay dependent and optimised for specific
clinical conditions, therefore may be improved with optimisation.
Biomarker Detection Range* Capture Antibody
Detection
Antibody
FABP Intestine 0.65 - 100 ng/mL BR115-F7G7 BR115-G6B9
FABP Liver 0.03 - 90 ng/mL BR106-G5A2 BR106-P9F5
G. lamblia not tested BR120-P3F2 &
BR120-T1G8
HSP60 0.1 - 1200 ng/mL BR100-G2A7 BR100-H4C2
Human Fetal Hemoglobin 1.9 - 305 ng/mL BR121-T5H9 BR121-V5A7
Leptin 3.9 - 691 ng/mL BR123-M4D6 BR123-P4G2
MMP7 0.1 - 293 ng/mL BR124-S4E3 BR124-T6G8
MPO 2 - 1200 ng/mL BR240-N5G6 BR240-D8B4
Neutrophil Elastase 0.008 - 33 ng/mL BR127-H2A4 BR127-G7C2
Proinsulin C-Peptide 0.09 - 38 ng/mL BR143-G5D3 BR143-K7C9
S100 Beta 0.04 - 5.6 ng/mL BR332-G4B9 BR332-F5H7
ST2 1.39 - 97 ng/mL BR139-S7B7 BR139-V8D4
ST2 0.08 - 31 ng/mL BR139-T3G5 BR139-V8D4
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