may 2015 antibodies newsflash - bbi solutions
TRANSCRIPT
Antibodies Newsflash May 2015
New Antibody Data
Troponin I Data
CEA Pairing Data
PBP2a Data
Capabilities
Monoclonal antibodies
Polyclonal antibodies
Recombinant Fab antibodies
Quality Innovation Results Partnership
Be confident in your results when you
choose BBI’s monoclonal antibodies for
your next assay:
Tested in ELISA
Tested in lateral flow
Recommended pairings provided
Positive customer feedback
We have now introduced highly sensitive
recombinant Fab antibodies to improve
your next generation assays
New this month:
Data on Troponin I, CEA and PBP2a
antibodies
Why choose BBI? Quick Links
Troponin I Data >
CEA Data >
PBP2a Data >
CRP Recombinant FAB
Data >
D – Dimer Data >
HbA1c Data >
Monoclonal Antibodies >
Polyclonal Antibodies >
Recombinant Fab
Antibodies >
How to Order >
Antibody Spotlight NEW DATA – Troponin I Lateral Flow Data
Detect down to 12.5ng/ml Troponin I in lateral flow using BBI’s lateral flow antibodies
Epitopes matching those prevalent in Troponin I assays
The antibodies are also suitable for ELISA
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Capture: BM322-K5F1 & BM322-N4D1 (0.8 mg/ml)
Detection: BM322-M4E5
TnI (ng/ml): 0 12.5 25 50 100 200 0 12.5 25 50 100 200
Capture: BM322-K5F1 (0.8 mg/ml)
Detection: BM322-M4E5
Antibody Epitope AAs
BM322-K5F1 QPLELTGLGFAELQDL 81-96
BM322-M4E5 LDLRAHLKQVKKEDTE 167-182
BM322-N4D1 EVGDWRKNIDALSGME 187-202
Antibody Spotlight NEW Pairings / DATA – CEA
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Capture Antibody Detection Antibody Application
BM220-P3C5 BM220-D7C6 ELISA
BM220-P3C5 BM220-F5H1 ELISA
BM220-E1E3 BM220-E7E2 ELISA
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
2
0 0.781 1.563 3.125 6.25 12.5 25 50
CEA Antigen Concentration (ng/ml)
Absorb
ance A
480
ELISA Showing Range of Detection With New CEA Antibody Pairings
BM220-P3C5 (c) vs BM220-D7C6 (d)
BM220-P3C5 (c) vs BM220-F5H1(d)
BM220-E1E3 (c) vs BM220-E7E2(d)
Our new CEA antibody pairing can detect the presence of CEA antigen down to
0.5ng/ml or lower
Antibody Spotlight NEW DATA – PBP2A (MRSA)
Our recommended matched pairing for PBP2A combines a capture antibody with very low
Protein A binding (BM306-M3D1) with a detection antibody with very high affinity for PBP2A
(BM306-O3G7). These antibodies detect MRSA and do not detect MSSA.
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Antibody Type Properties
BM306-M3D1 Capture Very low Protein A binding
BM306-O3G7 Detection Very high affinity for
PBP2A
S. aureus strain
OD
Sandwich ELISA
Capture: BM306-M3D1
Detection: BM306-O3G7 (HRP-conjugated)
Antigen: lysates of S. aureus strains.
Antibody Spotlight NEW DATA – CRP Recombinant FAB
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0
200
400
600
800
1000
1200
10
0
75
50
25
10 5 2 1 0
Peak h
eig
ht
CRP conc (ng/mL)
Comparison of BR228-D4A3 with commercially available antibodies
BR228-D4A3
Competitor A
Competitor B
Flu Fab
Reader
CRP conc (ng/ml) 100 75 50 25 10 5 2 1 0
BBI antibody, BR228-D4A3
Competitor B antibody
Competitor A antibody
Visual
Lateral Flow: Comparison of BR228-
D4A3 with commercially available
antibodies
Our CRP antibodies have a dynamic range of antigen detection of 151 - 23100 ng/ml
determined by ELISA. Our CRP antibodies can be used in a competitive ELISA, without
need for pairing. Alternatively they can be paired with other antibodies.
BR228-D4A3 antibody, is able to detect down to < 2ng/ml in lateral flow allowing you to
produce high sensitivity assays. We have determined the affinity constant for this
antibody to be 0.138 nM.
Lane 1: Native Human
D-Dimer
Lane 2: Human
Serum
Lane 3: Native
Fibrinogen
Lane 4: Native
Plasminogen
D-Dimer clones 1D2 and 3B6
3B6 clone used in a number of
commercially available assays
Tested in lateral slow, sandwich ELISA,
Western Blot and Latex Agglutination
Clones cited in 100’s of peer-reviewed
journal papers
Do not detect the closely related
molecules Fibrinogen and Plasminogen
Product Code Description
BM243-1D2
Sandwich ELISA: Detection Ab paired with BM243-3B6 as Capture
Lateral Flow: Capture Ab paired with BM243-3B6 as Detection
Western Blot
BM243-3B6
Sandwich ELISA: Capture Ab paired with BM243-1D2 as Detection
Lateral Flow: Detection Ab paired with BM243-1D2 as Capture
Latex Agglutination
Western Blot
Antibody Spotlight D – Dimer Monoclonal Antibodies
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Antibody Spotlight HbA1c Monoclonal Antibodies
Product Code Description
BM300-F7C2 ELISA - STAR PAIR: Detection antibody paired with BM302-F1B4 (Hb mAb), or
paired with BM302-S6B9 (not available for sale in USA)
BM300-H2E2 ELISA, Lateral Flow & Turbidimetric Assay : Detection paired with BM302-S6B9 (Hb
mAb)
Some antibodies are currently not available to be sold into the USA. Return home >
BM300-F7C2 is able to quantify
levels of HbA1c in patient blood
samples using an ELISA assay,
correlating with results from a
commercial HPLC platform (Biorad
Variant II). (Star Pair)
Our Capabilities | Monoclonal Antibodies
Cardiac Infectious Disease Listeria Veterinary
D-Dimer C. difficile Toxin A Malaria HRP2 Canine Distemper Virus
Myoglobin C. difficile Toxin B Malaria LDH (pan) Avian Influenza H5
Troponin I Chikungunya Malaria LDH (Pf & Pv) FeLV
Dengue NS1 Norovirus Brucella LPS
Diabetes Ebola Reston PBP2a Enrofloxacin
Hb Ebola Sudan TB Goat IgG
HbA1c Ebola Zaire Canine IgM
HAV Tumour Chicken IgY
Drugs of abuse H. pylori CA125 GST
THCΔ9 HAV CA15-3
HBV CA19-9
Hormones Hepatitis B Calcitonin
Progesterone HIV p24
PTH HIV gp36 Immunoglobulins
HIV gp41 IgA
Pregnancy Influenza A IgE
AFP Influenza B
Leptospira
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Our Capabilities | Polyclonal Antibodies
Inflammation Pregnancy Renal
CRP AFP A2M
B2M
Hormones Inflammatory Cystatin C
HCG A1AT
αHCG
Haematology Metabolism
Immunoglobulins Haptoglobin Ceruloplasmin
IgA Ferritin
IgM Haemoglobin
IgG
Albumin
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Our Capabilities | Recombinant Fab Antibodies
* Detection Ranges are assay dependent and optimised for specific
clinical conditions, therefore may be improved with optimisation
Biomarker Detection Range* Capture Antibody Detection Antibody
HSP60 0.1 - 1200 ng/mL BR100-G2A7 BR100-H4C2
Adiponectin 0.1 - 525 ng/mL BR101-P7E2 BR101-M3H8
Adiponectin 230 - 59118 ng/mL BR101-H2B4 (Competitive ELISA)
Angiopoietin 2 0.001 - 97 ng/mL BR103-P4E8 BR103-V5F2
Big Endothelin 1 0.001 - 1.8 ng/mL BR104-D6A4 BR104-H1A4
Calcitonin 0.006 - 2 ng/mL BR388-G6B1 BR388-D5C4
Cathepsin D 0.03 - 150 ng/mL BR107-F5G2 BR107-K6D3
Eotaxin 0.007 - 2 ng/mL BR108-M7H3 BR108-K2D9
Chondrolectin 0.9 - 21 ng/mL BR111-P8G3 BR111-T6B1
CKBB 0.15 - 33 ng/mL BR112-V6F6 BR112-Z1C4
Proinsulin C-Peptide 0.09 - 38 ng/mL BR143-G5D3 BR143-K7C9
CRP 151 - 231000 ng/mL BR228-D4A3 (Competitive ELISA)
Cystatin C 165 - 9073 ng/mL BR175-G8D9 (Competitive ELISA)
Cystatin C 165 - 9073 ng/mL BR175-H4C8 (Competitive ELISA)
E. histolytica not tested BR116-K9F7 BR116-G3A1
E-selectin 0.05 - 235 ng/mL BR117-K2E7 BR117-H5B6
FABP Intestine 0.65 - 100 ng/mL BR115-F7G7 BR115-G6B9
FABP Liver 0.03 - 90 ng/mL BR106-G5A2 BR106-P9F5
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Our Capabilities | Recombinant Fab Antibodies
* Detection Ranges are assay dependent and optimised for specific
clinical conditions, therefore may be improved with optimisation
Biomarker Detection Range* Capture Antibody Detection Antibody
G. lamblia not tested BR120-P3F2 & BR120-T1G8
Human Fetal Hemoglobin 1.9 - 305 ng/mL BR121-T5H9 BR121-V5A7
Leptin 3.9 - 691 ng/mL BR123-M4D6 BR123-P4G2
MMP7 0.1 - 293 ng/mL BR124-S4E3 BR124-T6G8
MMP9 Active 11 - 183 ng/mL BR125-N5F7 BR125-M6D6
MPO 2 - 1200 ng/mL BR240-N5G6 BR240-D8B4
Neutrophil Elastase 0.008 - 33 ng/mL BR127-H2A4 BR127-G7C2
Free PAPP A 0.3 - 299 ng/mL BR129-M5E3 BR129-F2H4
Pentraxin 3 4.6 - 876 ng/mL BR130-H2C3 BR130-K6D6
PGDS 0.2 - 85 ng/mL BR131-M6H7 BR131-P7F7
PSAP A 0.013 - 123 ng/mL BR132-T5G4 BR132-S6E4
PSAP B 0.3 - 1500 ng/mL BR133-V6B5 BR133-V4D1
RBP 4 350 - 500000 ng/mL BR134-Z7D3 (Competitive ELISA)
S100 Beta 0.04 - 5.6 ng/mL BR332-G4B9 BR332-F5H7
ST2 1.39 - 97 ng/mL BR139-S7B7 BR139-V8D4
ST2 0.08 - 31 ng/mL BR139-T3G5 BR139-V8D4
Tie2 0.8 - 600 ng/mL BR140-Z7A5 BR140-D9D7
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How to Order
Please get in touch to arrange a quote for
any of our products.
You can also order directly via our website
www.bbisolutions.com
USA: 1-800-423-8199
Intl: +44 (0) 2920 747 232
Quote on ordering – NF MAY
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