biofertilizer and bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · dr. zulkini hj....

12
Production of Bacterial Inoculants as Biofertilizer and Bioenhancer n·/: +603 89-16 6990 c-lIIail: -lIhholll({J 0 'ri.rl 1111.<,£111.",,- f)r'/UrI'llIIe", oj UlllrI A!rlllL/!ll!lIIr'r11 /'"oclI/rl' oj A U"iI'<!rI;t; P"tra Mo/anio -I3-1()() UPM, I!nltlll!l' Sdrlllgnr l/tr/o.'"';tr UPM R&D Dlges , Issue 3. 4rh Ouarter (Dec. 2003) In conclUSIon, these results strongly Indicate the vlabihty of usmg B. sphaericus UPM 10 With GOPF and KC as Inoculant earners to commercially produce a bactenal moculant for various economic crops, namely or! palm and bananas. @ higher acetylene-reduction assay (ARA) actiVIties (129 pmole planr l houri) and mcreased nutrient uptake. Synthesis Results showed that BaCillus sp UPMB 10 was able to grow and multiply m COif dust (CD), ground 011 plam frond (GOPF) and KUSOKOM compost (KC) at all temperature treatments (20 ,30 ,40'). GOPF supplemented With 25% KC showed highest growth after 5 ' /2 months (10 8 dulg moculant) when stored at 30'C and mOisture potenual pF 2 54 Inoculation test With 011 palm seedhngs m VitrO showed an accumulauon of 47- 67% N from atmosphere (Ndfa); rhizobactenal moculation contributed 30% Ndfa (350mg N plant I 260 days·l) through N flxauon. PGPR Inoculant also mcreased groWlh of root (42%) and top (19%). For banana, the root of PGPR-moculated plants produced Zulkifli Hj.Shamsuddin and Premalatha Pakirisamy In MalaYSia there are hmlted sources of bacterial.noculants as plant blofertlh2ers and bloenhancers. Plant groWlh-promoting rhlzobacteria (PGPR) such as Azospirillum and Bacillus spp. can be safely used as moculants to enhance plant growth, frx Nz and decrease rehance on Inorganic nltrogenous fertlhzers. Several experiments were conducted to produce the Inoculant namely the Isolation of bacteria, the carner and ItS shelf hfe, and the efficacy on economIC crops

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Page 1: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

Production of Bacterial Inoculants as

Biofertilizer and Bioenhancer

n·/: +603 89-16 6990c-lIIail: -lIhholll({J 0 'ri.rl 1111.<,£111.",,-

f)r'/UrI'llIIe", ojUlllrI A!rlllL/!ll!lIIr'r11/'"oclI/rl' ojA ~riCII//IIn'

U"iI'<!rI;t; P"tra Mo/anio-I3-1()() UPM, I!nltlll!l' Sdrlllgnrl/tr/o.'"';tr

UPM R&D Dlges , Issue 3. 4rh Ouarter (Dec. 2003)~

In conclUSIon, these results strongly Indicate the vlabihtyof usmg B. sphaericus UPM 10 With GOPF and KC asInoculant earners to commercially produce a bactenalmoculant for various economic crops, namely or! palmand bananas. @

higher acetylene-reduction assay (ARA) actiVIties (129pmole planr l houri) and mcreased nutrient uptake.

Synthesis

Results showed that BaCillus sp UPMB 10 was able togrow and multiply m COif dust (CD), ground 011 plamfrond (GOPF) and KUSOKOM compost (KC) at alltemperature treatments (20 ,30 ,40'). GOPFsupplemented With 25% KC showed highest growthafter 5

'/2 months (108 dulg moculant) when stored at

30'C and mOisture potenual pF 2 54 Inoculation testWith 011 palm seedhngs m VitrO showed an accumulauonof 47- 67% N from atmosphere (Ndfa); rhizobactenalmoculation contributed 30% Ndfa (350mg N plant I260 days·l) through N flxauon. PGPR Inoculant alsomcreased groWlh of root (42%) and top (19%). Forbanana, the root of PGPR-moculated plants produced

Zulkifli Hj.Shamsuddinand Premalatha Pakirisamy

In MalaYSia there are hmlted sourcesof bacterial.noculants as plantblofertlh2ers and bloenhancers. PlantgroWlh-promoting rhlzobacteria(PGPR) such as Azospirillum and

Bacillus spp. can be safely used as moculants toenhance plant growth, frx Nz and decrease rehance onInorganic nltrogenous fertlhzers. Several experimentswere conducted to produce the Inoculant namely theIsolation of bacteria, the carner and ItS shelf hfe, andthe efficacy on economIC crops

Page 2: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

EDITORIAL

Working together to raise standardsThe (PTA Rc:.can:h and o.:vdopm~nI E~po.-.itioll 2003 joint!) U1'B:lni ....:d by Mlnh,r)' alEducalion ~1111:l) ...ia and Public In\liIUllon~ of Hi{!her Learning anu OO\h..-d by Uniw"itiPulro Mallly)ia fUPMI fmm 9-11 Oclnher at PWTC. Kualu Lumpur added anolhcr fe:uheron 10 the UPM') hut. It wa., Olll.:e a~tlin Ii proud moment for Unih:.....ill PuIJ'3 Mlllay... t3 "'hl:n10 of il~ .!oCicnli~l' y.e.re (kt:larcd wjn~" anti aw:mled medals in tne IP7it Ri'Si'I"rh &Di'l'elopml!1l1 £rptl.t;timllO()J in difTerenl cutegorics.

Dr. ZUlkini Hj. Sham'iudJin. Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s

declared the. Malll)'.;ian Society of Plant Ph)"siology (MSPPI top ",'inner for hI' research(milled. "Production of RacleriaJ lnoculan~:b Biofcr1ili7.cr and Bioe:nhanccr~.

Dr. Mll7.iah Mahmood. Pro(~ of Bioch(mbuJ and Microbiolog) also won lheMala)"iian Society of Plant Phy...iolOID' eMS??) 'MERIT' award for her I\l.O re"':3n:hprojects cnlllkd "Comnlt:reial ProdUClion of Eoonomicall} Imponant Planling I\.latcrial'Uo;ing Ti..''Uc Culture Tcchnologie~" and "Genelic Engineering of Ban:uus for Tolct:lnC(' 10Fusarium Will Disc:L~". In addition. Professor Maziah also won:ln :lward under the PlantQefl('tic C'dtcgOl) (Malay~ian Genetic Sociclyl forhcr re!>e3rch entitled "Gencl~

Engineering of Ban:tnas for Toler.l~ 10 Fusarium Wilt Di-.e:L'iC".

As-weiBte Profe"-.or Dr. Abdul Rahman R:llnli fmm F3rulty of Engine.:ring \\on the'PIKOM :tWllrd for his res.:arch on SMS Gateway Interface.

ProfeSsor Dr. Ahmad Soid SaJnp from Facully of Forcstry won:t tIolalay"i:m Plant ProlectlonSociCly (MPPS) Gold medal for his re\Carch in"~ntioo 00 Dc:\clopmcnt of BiojX'Sticidc forMlln:tging Cruciferous Vegctnbk PC.SI.

A'isociate Profes...or Dr. Hiryali Abdullah fmm Faculty of Ai,!riculture won a MalnysillnPlant Prolection Society (MPPS) Bronze medal for her research on An IntegruledManagement for the Control of 11:Ict~rial Wilt ofTolllalo Caused by RlIlwUllifl

SOI(IIUJ(·t!flrJIlI.

Dr. Abdul Hlllil1l Sh33J'i. Profc.s~rof Physics from the Faculty of Science andEnvironmenlal Sludie,\ wun:l MlIl:lysinn Solid State Science & Technology Society(MASS) 2003 award for his research on 1..01\1 Field Magnelorc.,islanee of M:l1lgunih:\Pe(()\'sl;ite,.\ Hulks ,md Films for Sensing Devices.

Profe.\sor Dr. Radin Unmr Radin Suhadi lind Associate Profc.\sor Dr. Fakhrn'I-Ral.i BAhmadun from the Faculty of En1!ineering al..o recd\ed lhe Malay~ian Solid Slllle ScienL'e& TI."Chnotogy Society (MASS) 2003 awards fOf" their l'CSCarch entitled ~Dc,ign of Co,IEfTecti\'l: anti Comfonuble MOlon:ycle BeimCI With Bio--Com~ile Malenal for TropklllClimatcs" nnd "Cnrbon NaoolUbcs nnd Carbon Nanofibers Synthl:si' for Nanolechnol~)'l\pplicalion~" ~pecti\'CIy,

Associnte Professor Dr. Rozil:l Rosli from Faculty of Medicine and Heal!h Sciencc.~ wonlhe Malaysian Ge~IIC Sociely award undcrlhe 'Microbe (]enelic- category for her ~an:hentitled. "Cholc(3 DNA Vaccine" and Dr, Jothi Mnlar Panandam fmm thi: Facully ofAgricuhuR: :'110:0 won the Malay,,;an Genelic Soci.:ty award under the 'Ammal Gene-Ii/:'calegory for her rcsc:uch on Gen('lic Variability of Four SheepB~ in Malay,ia.@

Executive [email protected]

EDITORIAL BOARDAdvisor Professor Muhamad Awang

Co-Advisor A.Prof. Mohd. Shahwahid Othman

Executive Editor A.Prof. Sidek Hj. Ab. Aziz

Managing Editor Nayan Deep S. Kanwal. Esq.

Reviewers A.Prof. Zulkifli Idrus.A.Prof. Nor Aripin Shamaan

Secretary Jamsari Tamsir

Circulation Manager Mustapha Kamal Tahir

Reporter Siti Nurulhuda Abu Seman

Online Webmaster Ahmad Faisal Abdul Ghafar

Assistance Nurhaznita MahmoodSaharia Mohd JaniZaihan Yazid

f1 Synthe.;s. UPM R&D 0'9"'1, ",uo3. 4.h Qwrter(Oec.2003)

SPOTLIGHT

Academic WritingIf the reader is to grasp what the writer means,

the writer must understand what the reader needs

Science is oflen hanlto read. Mo~t people a..sumc that it.. difficulties are

born oul of necessity. out of Ihe cxtremc complcxily of scientific

concepts. data and analysis. Complexity of thought need not lead to

impenct,dbility of expression: number of rhetorical principles can

produce clarity in communicalion wilhout ovcrsimplifying scientific

issues. The results arc subslantive. not merel) cosmt:tic: Improving the

qualily of \\'riling actually improves the quality of thought.

The fundamemal purpose of scientific discourse is not the mere

presentation of infonnalion and thoughl. bUI rather its aClual

communication. It does nOl mailer how plcOl.!>Cd an author might be 10

ha'-e cunvened allihe right dalO! into sentenCl.'"S ami paragraphs; il mailers

only whether a large majority of the reading audience accurately

perceives what the author had in mind. Therefure. in order to unr.h:rstand

how besllO improve writing. we would do wcllio undersland bener how

readers go about reading.. Such an underslanding has reccnll)' become

available through work done in the fields of rheloric. lin!!uislics and

cognitivc psychology, II has hclped 10 produce a methodology based on

Ihe concept of reader expectations.

Let us begin with Ihe statement that, as far as the language is concerned.

there is no difference between Academic Writing (AW) and ordinary

writing. It is governed by all rules of gr.lIntnar of the language (English).

There arc sentences wilh subject. verb and object (adverbs. adjectives.

elc.).

• Therefore. Academic Writing is an an of "'riling.

• It depends on the writer and the inlenlion. Academic Writing is

definitely different from those wrillen for making love. joking or a

polilical speech. Academic Wriling is serious business. recording

eVents in a systematic manner and ",rillen by members of the

aC<.ldemic community whether Ihey are professors. leclurers. scientisLS

or university gradu<.ltcs to cOIl\'ey their contributions 10 knowledge.

• I am sure that there are many who can boast or talk about their

achievements hUI whcn :L<;kcd 10 PUI dm...n in '..-riting will not hesilate

10 repl)' - busy no time. Why? Oncc in writing you can be

challenged!

• It is the abililY 10 write (communicate) will detcnnine your success or

failure within the scientific community,

• You may be a good re~earcherbUI your im'cstigation, are incomplele

without puhlishing the result... Many rcsean::h project<; remain

unpublished because Ihe researcher is unabh: to wrile.

• AW deals with pUlling your thoughts. findings into print for olhers to

know whal you have done or doing in prinled form - Publications­

similar 10 Ihe contributions of an anisl.

• You can document your research in the fonn of a thesis. dissenations.

journals. proceedings of conferences. books. chaplers or consultancy!

technical rcpons bUI because of Ihe "cry rigid forntal of these

publishers. mosl researchers fail to gCI their findings published.

• Academic Writing is not diffi"ult 10 acquire if )'OU follow the

guidelines of the publisher and re<.ld the documenls thoroughly. you

will find lhat the)' are well strul'turcd and your atJility 10 write will

gradually improve with the passage of time.

• ICT ha<; revolulionised Academic Writing. MS word is m:ommended

for you 10 improve your English. spelling. and ,hoice of word<;. 11

even helps in your gramm:lr and Ihesaunls

• To gel surprising idcas. extensivc reading is imponant. Young people

fail to realise the impnnance of it. You c,tnllot just sit there looking at

your apparatus. You have to rend a lot before you gel new ideas! ;We

Managing Editor

~

Page 3: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

..",/1,\1,,1

Deparllllt!1I1 ufChelllical & EIJI'. EIIKilleaillgFaCIlity of £lIgillt!('rillgUllin'r,~iti Pw/'(/ Malaysia43400 UPM, Senlcm.li, SelcmgorMalaysia

Tel: +603 8Y46 6268£-IIIail: J(/1J1I'I"(~ ('/J.f!.,lIP11l,t'tftl.JlJ~

II'Ii S/-;R If 'II \ltllI \tllid .\Itl/i' CIl'lIe, ,~

To JI"I, '\ \"." 1\ (If I '\ ) 1\1 art! 1I'r.1 N, \I ((1'( h, ' /I, ... I"I'IIIt /1/ I '/'tI (//"" 2011.1,

Engineering, University Putra Malaysia, have beenvery impressive and encouraging. Being first timeproduced In Malaysia, carbon nanotubes producedIn our labs uSing our new design of CVD haveshown very high quality and astOnishinglyconsistent. and comparable With other researchersworldwide who produced carbon nanotubes. Othercarbon nanomatenals produced Include carbonnanoflbers, carbon nanopartlcles, and a newmaterial we named carbon nanoporous balls.@

Not only to speak of the reduction of cost and timeachieved at UPM, the availability of the CVD willboost the research in the field of nanotechnology inMalaysia as this Will encourage lots of researcherswho lack the facility and funding to run and startsimilar researches. A Malaysian patent has beenfiled for the UPM CVD. We look forward toindustrial collaboration to further enhancenanotechnology R&D for the country,

50 far, the outcomes of thiS research at theDepartment of Chemical and Environmental

than steel but It IS 6 times lighter. ThiS researchrequires the setting up of an equipment called theChemical Vapour DepOSition «VD) unit. As thistype of research did not exist in Malaysia before,such equipment (i.e. CVD) was not available.Therefore, as a start, the Idea of bUying the CVDwas a first chOice, however the exorbitant cost ofthe equipment (- RM 900k) beSides the excessivelime taken to deliver it from overseas have spurredthe group of researchers to develop their owndesign of the CVD and build it from locally availablematerials. Thus, the cost (- RM lOOk) and time ofthis research have been dramatICally reduced.

RESEARCH UPDATE

There are many methods of producing thesenanomaterials, including electric arc discharge, laserevaporation. chemical vapor deposition (CVD), andplasma-enhanced CVD, among many others.Among these. CVD seems to be the most promisingmethod for possible industrial scale-up due to therelatively low growth temperature, high yields andthe high purities that can be achieved. Thesenanomaterials have a range of promiSing properties,Including unique mechanical and electrical behaViorand are under investigation for a Wide range ofpractical applications such as field emission displays,hydrogen storage, nanocomposites, and fuel cells.

Though In MalaySia this kind of research IS relativelynew. and if no initiative is taken by the researchershere, Malaysia will be left behind in thiS field oftechnology and will be totally dependent on foreignsuppliers for its needs in this area. This research onnanotechnology IS the door for many coming andpromiSing technologies and their enormousbeneficial applications, In order to follow up withthe existing global trend in technology, theDepartment of Chemical and EnvironmentalEngineering, Universiti Putra Malaysia, initiated aresearch on carbon nanotubes production InOctober 2001. Carbon nanotubes and carbonnanofibres are the building blocks ofnanotechnology research. Carbon nanotubes canexhibit tensile strength of upto 100 times greater

Research on new matenals technology is attractingthe attention of researchers all over the world.Developments are being made to improve theproperties of the materials and also to findalternative precursors that can bestow desirableproperties on the materials. Great Interest hasrecently developed in the area of nanostructuredcarbon materials. Carbon nanostructures aregaining considerable commercial importance withinterest growing rapidly over the decade or so sincethe discovery of Buckmlnster fullerenes, carbonnanotubes, and carbon nanofibers.

Design and Fabrication of CVD (Chemical Vapour Deposition)

for Nanotechnology Research(Carbon anotube and Carbon anofibre Synthesis)Fakhru'I-Razi Ahmadun, Muataz Ali Atieh, Iyuke Sunny Esayegbemu.Maan Fahmi Alkatib, Dayang Radiah Awang Biak,Abdul Ghani Liew Abdullah, Faizah Mohd Yasin,Mohd Halim Shah Ismail and Abdul Hamed Belal Danna

REGULARS

Pest

....DC'porrlllem of ForeSI MmwgC'lIIl'lIIFaCility ofFore~")'U"i"ersiti PWf'(J Malaysia-13400 UPM. Serdallg. SelallgorMa/tIl',Vio

Tel: +60389-167/9-1

\1."11 \UI' I'll ,JII',."tt (/umfl1l'I'~ ~ ("/ IP/IN,,,'trb. [),I \/'" "",11 '0(1

The result shows that mortality SignificantlyIncreased by 47% from 53% when the larvae werereared at 20·C to 100% at 30 and 35·C The ITsovalues decreased from 24 to 6 days. All virussuspension used in the expenment inducedmortality to more than 90% except in pH 11, whereIt was 64% and 100% mortality was recorded frompH 7. The lTso increased to about four folds whenthe pH of suspension was Increased from 7 to 11.5pltNPV was, however, adversely affected byUV-Iight. laboratory and field trials usingUV-protectants show that the virus remainedInfectiVe and subsequently gave a comparable yieldof Brassica rapae as compared with that of thechemically treated crop.

I

This research reveals the potential of anucleopolyhedroVirus for development of abiopesticide for managing cruciferous vegetablepests,

In this study an armyworm Spodoptera litura(Fabricius) (lep., Noctuidae), an important pest ofmany crops including cruciferous vegetables, wasselected. The virus, coded as 5pltNPV, originallyIsolated from Telong, Kelantan. was tested for itsInfectivity to the armyworm at differenttemperature, pH and ultraviolet radiation.

Development of Biopesticide for

Managing CruciferousVegetableAhmad Said Sajap,Mohammad Abdul Bakir,Hussan Abdul Kadir andNorani Abdul Samad

Page 4: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

RESEARCH UPDATE

Genetic Engineering of Bananas for Tolerance to

Fusarium Wi t Disease•...,

. '

D

c

G

I'/ale 3. f. \J .\lul/if/h· '''(1111\ H'ffl' {C/1lU'iW',IIlIW Utili''''' tI tIni/ lIu,,"wim.·t/ m IlA/~"tJlmHllt' (Ill'f",,, .\mgt Illwultl[ /t;',\;Htm,

III (l1I(ilno'It '·r/(',.(um" t"" ([('\'('/O/IC'c/ tfUll ('IJI'/inm. tllrrull pel< (Iud S""tht'll1 81m OIlt1h ft',\ .re) IlIortt/rwl\ uf {""w,,.ittm('tmitl'cll \11\1'('11.\11i1l In TTcm, l('mt' l'fIlWlrtJ.(/») 1ml"'I!f'llIt lumaua ("/l'ralln'\ 10 Fu\tlnum h'lIt ,1i\t"U.fl·.

PlaIt! 2. PC Ii tlllil SOlll/lt'rl! hI"t ilJllf/\";' fJ/ Iht' t"hilllltl\~' (111111\- J.3 glm'/Uw.\t· g"",,\ IIJ (rtlfl\"l"lJh' htultlllcll'lwlIl,'/o,;

]')/ale 1. \ "utll"m;,,,, flf Hill,/( l.:u\A (. \. C. r tllld (j) tlllil 'II' (If. n r flnd II} ttt'II('( ("I'Tcnhm ill "lIr;ml\ /;t.\lll'.\ (1//)'I/I,UI/I \ al/l/ lJ.. Singh III/d.\, ( .lI/tI/t· I/ul/ll'!<' /Iutl dUll/I" (.I/bnl, Land f"' u(JI'n: (j "I/I/II'/I,IO{\

Maziah M , Sreeramanan 5.,Abdullah M.P" and Sariah .M.

An effective method of controlling Fusarium wilt

is by planting new tolerant varieties.

Conventional breeding is hampered by high

sterility, polyploidy and long generation time of

most cultivated banana cultivars. Genetic

engineering offers as an alternative route to

increase resistance to pathogens in plants.

Establishment of a reliable transformation and

regeneration system is essential for genetic

engineering. Biolistic was initially chosen as the

method for banana transformation using tiny

single meristem buds as it has been the most

successful transformation system for

monocotyledon plants. Optimisation of physical

and biological parameters, including testing of

selection agents (kanamycin, basta, hygromycin ,

geneticin G-418, neomycin and paromomycin),

was carried out as a prerequisite for stable

transformation. This has resulted in the

successful transfer of reporter genes (GFP and

GUS) into banana, thus making it possible to

improve banana via genetic engineering. Besides

application of the Biolistic method, a study on

transformation-mediated system by

Agrobaeterium has also been carried out.

Fusarium Wilt disease causes severe damage in

commercial plantations and represents a major

constraint to banana production in Malaysia.

Control measures involving field sanitation, soil

fumigation, organic amendments and crop

rotation are non-effective.

MERiT IIlalaniol/ ociely of PilI/IIPhysiology (lvISPP) ,\ward IPTA !?e.\eorc!t

& OC1'('/opmell1 £.rpO.l"ilioll 2003.

WI ER Malay,~ial/ Genelic ·odel.\' AWl/I'd

(Plalll Gellelic cG/egoll') IPTA Research &[)cl'elopl//l'1I1 £.\[HJ.\'ilioll 2003.

Deparlmetl/ ofBiochemistry and Microbi%gyFacIIII)' ofScience and Environmental Sludj,'sUniversiti Plt/ra Malaysia43400 UPM, Serdang, SelangorMalaysia

•Tel: +6038946 6703E-mail: llIar;]ah@4cls,u/2llL£flu..nJy

Genetically modified Fusarium wilt tolerant

bananas produced would overcome many of the

risks associated with pesticide uses and they are

safe for consumers.

estimated copy number of inserts were detected

by Southern Blot hybridizations. Plantlets from

the chitinase and ~- , ,3-glucanase positive plants

were tested for their resistance to the Fusarium

oxysporium f.sp cubense spores using optimised

conditions. The degree of resistance displayed by

these transgenic plantlets was correlated with

the level of the genes expression and different

gene combinations.

In our attempt to optimize and improve

Agrobaeterium-mediated transformation, several

parameters were evaluated based on GFP and

GUS transient gene expressions. Upon extensive

revision of the regeneration and improved

selection protocol, transgenic bananas were

produced by use of two different Agrobacterium

strains (EHA 101 and LBA 4404) using chitinase

and ~-1,3-glucanase genes. The majority of

transgenic banana plants produced were

morphologically normal and independent

transformants have been confirmed by the

Polymerase Chain Reactions (PCR) to contain

reporter genes, selectable marker gene (npt 11)

and the antifungal genes. The presence and

~ Synthesis UPM R&D Digest,lssue 3, 4th Quarter (Dec.2003)

Page 5: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

Cholera DNA Vaccine

REGULARS RESEARCH UPDATE

Rozita Rosli, Mariana Nor Shamsudin, Abdul Rahman Omar,

Shahrilnizam Abdullah and Lama Hamadneh

In circumventing many of the drawbacks of

traditional cholera vaccines, a new generation

vaccine· a cholera DNA vaccine was

constructed. The present invention relates to a

plasmid (pVax) carrying a Vibrio cho/erae gene

(ctxB).

This approach of using DNA involves delivering

the gene sequence of the desired antigen into

the host by inserting the gene for the antigen via

a replicating plasmid vector. such that a strong

and broad-based immune response is produced.

Initially. expression of the ctxB antigen cloned in

pVax was achieved in vitro using COS-7, a

eukaryotic cell line. This was followed by in vivo

immunogenicity testing involving Balb/c mice.

The present invention was injected

intramuscularly into the mice in a simple saline

solution using a technique that had been

previously validated. Humoral response was

induced where IgG levels increased after the

booster injection. The present invention offers a

cholera DNA vaccine that demonstrates

protective efficacy in mice challenged with

clinical isolates of Vibrio cho/erae.

Hence. this gives a measure of success in

developing an effective cholera vaccine

applicable to humans.

Being a DNA vaccine rather than a protein, the

invention is inexpensive. stable, easy to produce,

does not need refrigeration-qualities that make

feasible its widespread use in developing

countries where cholera is still a problem. @

WIN ER l/tllt/nit/II (i1'n,'Ii< \/1, It'll' IImrd

1\1Iuol" (;,·nl'li,. ,.tIl,·!!O}.... ) /I'/'I /<""'t/,<11 &

1),01'1'10(1111"111 I.I"'II'I/;"n 21}()J.

..•.

Depal'/melll ojHllmall Growth & DevelopmelllFaclllly IIJMedicine and Heallil ciellces

Unh'ersiti PlIlra Malaysia43-100 UPM. SerdC/Ilg. Selcl1IgorMalaysia

Tel: +603 11946 11480E·mail: m~i/JJ@ml!d;~/tp.!.!l.~dll.m:r

Detection of lard in Chocolate by

r·er TransforITl Infrared (FTIR)

Spectroscopy for 'Ralal' Authentication

Yaakob B. (he Man, Syahariza Bt. Zainul Abidin, and M.E.S. Mirghani

Determination of food authenticity and

detection of adulteration are major issues in the

food industry, causing concern among

consumers and demand special attention among

food manufacturers. Lard is commonly blended

with other fats in food for economic reasons but

draws controversy among certain group of

consumers due to its implications on religion and

health. The Fourier Transform Infrared (FTIR)

Spectroscopy has proven to be a fast and reliable

technique in detecting adulterants in food. The

FTIR spectroscopy in combination with

attenuated total reflectance (ATR) and partial

least square (PLS) regression was used to detect

addition of lard in chocolate formulation. The

spectral bands associated with lard, cocoa butter

and their blends (ranging from 0 - 15% of lard

in cocoa butter) were recorded, interpreted and

identified. A semi quantitative approach is

proposed to measure the percentage of lard in

blends on the basis of spectral data at frequency

region of 4000·650cm·1 / using the equation

y = 0.9225x + 0.5539. The coefficient of

determination (R2) was 0.9872 with a standard

error (SE) of 1.305.

In this study, the potential of FTIR spectroscopy

as a rapid analytical tool for the quantitative

determination of adulterant especially lard in

chocolate is demonstrated. @

UI'II (,1l"'III;ml & Rel/',IIl·IIFIIIII>i/irlll :!(}03.

Department ojFood 7i!cll1IolngyFamily ojFood Science ond BiotecllllologyUn;I'ers;ti Purra Malaysia-13-100 UPM. Serdang. Selal/gorMalaysia

Tel: +60389-168371E-mail: \·[email protected]

Synthesis, UPM R&D Digest. Issue 3. 4th Quarter (Dec.2003)~

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THROUGH THE LENS

Researc

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~ Synthesis. UPM R&D Digest, Issue 3, 4rh Quarter(Dec.2003)

Page 7: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

•appenl g5

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Synthesis UPM R&D Dtgest. Issue 3. 4/h Quarter (Dec 2003) ~

Page 8: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

Abdul Halim Shaari, Lim Kean Pah, Hishamuddin Zainuddin, Chow Sai Pew,

Sidek Hj. Abdul Aziz, Noorhana Yahya, Abdullah Chik and K.K.Kabashiand Huda Abdullah

RESEARCH UPDATE

Low Field Magnetoresistance in Bulks and Films of Manganite

Perovskites for Sensing Devices

REGULARS

SlIperrOlulllcror alld Thin Film Labon/wryDepartlllellt ofPhysicsFOCI/It)' ofScience fllUl Em,jrolllllelllfli SlIIdie.~Unillersili Plllm Malaysia43-100 UPM. Serdallg. SelallgorMalaysia

f~igllre 6. \I .\1 mW~t olli/m

Tel: +603 89-16 66-18. 89-16 6873E·mail: aha/im@C\·a•. IIIJm.edll.lIl\·

WINNER \101/1\ ,;,11/ So/id 5tal/' 'nntl"(' &Ii'elllwlog\" S", iI'/I' (II \'is) \lnml IP'!it Rl'.It'l/rd,

& [)/'l'c/"I'"11'11I Erprl.li'/O" 21J03.

I;;gllre 5. SI .\/111;' h'~"'Jllt

Polycrystalline LSMO and LBMO films are

promising sensor elements that can be applied in

both low field (below 0.1 Tesla) and medium field

(0 to 1 Tesla) sensing devices. @

The wide range of particles size distribution as

shown by SEM (Figure 5) and AFM (Figure 6)

occurred in the films. which contributed to a

large number of grain boundaries, and hence

resulting significant LFMR effed.

JOO

-2.1micrCln- -1. OlirrCll

5 +==~~---r---.-----1100

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150 200 250

TCml'Cl"'dlurc (l\.)

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::::: 15

O! 0.4 06 0.8

'b~nCII lieh!. II (I )

·12+--~--~-~--~---l

Polycrystalline films show similar properties given

by bulk samples. However. the CMR values for

the thin films are not thickness dependent

(Figure 4).

...;.,

G

10

20...--------------,

Figure J. (":"N "Il/m'tH a JUlie-lUll! 0/ JIIug",'IIt" Jil'/,/ trl

r{lntlll,\ (ClJlp"I"t,lmn /,.,. u,~. r·.\1Il·() JIlIU

Figure 2. P"hC'III.Ll\I" J)"/J(J.\"illtJlI C;nft'm

Lal.xSrxMn03 (LSMO) and Lal.xBaxMn03 (LBMO)

bulk samples show significantly large CMR value

of 4% (Figure 3) and 6% at an applied field of

0.1 Tesla measured at room temperature. When

the applied field is increased to 1 Tesla. the CMR

value increases slowly to 8% (Figure 3) and 11 %

respedively. These samples show strong LFMR

effed, where MR value increases with the

decrease of temperature below Tc.READ WRITE

Figure J. Rl'tIIl-",r"L I".'{/(/ \t'1I\(II lmm:t (Jf,IlI1f1t'" ImmIfJM iI"IIII'1ItJ~.,

Manganite perovskite. which exhibit Colossal

Magnetoresistance (CMR) effed. is one of

candidates that offer promising properties to

utilize as the magnetic field sensing head.

Monocrystalline manganite perovskites materials

show intrinsic CMR effed arising from the

Double exchange (DE) where the maximum CMR

value occurred near Curie temperature (Tel and

metal-insulator transition temperature (TMI)

concurrently. In polycrystalline manganite.

another contribution of extrinsic CMR occurred.

due to the grain boundaries effed. namely Low

Field Magnetoresistance (LFMR). In this work,

polycrystalline La l.xAxMn03 (A=Ca.Ba.Sr) of

bulks and films have been prepared via solid

state readion and Pulsed Laser Deposition

techniques (Figure 2) respedively.

In the current technological revolution where

eledronics devices continue to decrease in size

and cost, the need for smaller, low-cost and

high-performance sensors dramatically increases.

The capacity of the hard disk in the computer

grows dramatically in the last decade. As the

capacity of the memory drives get higher. the

stored bits of data get smaller. Therefore, their

magnetic field gets weaker and making the bits

harder to deted and read. The key is to make

magnetic sensor head (Figure 1) that can read

smaller and lower magnetic field.

~ Synthesis. UPM R&D Digest. Issue 3. 4th Quarter (Dec.2003)

Page 9: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

Robiah Yunus, Fakhru'l-Razi Ahmadun, Ooi Tian Lye,

Azni Idris and Sunny M. Iyuke esters. The presence of hydroxyl group has

POSitive effect on wear and fnctlon as well as

OXidative stability.

Oil

RESEARCH UPDATE

EnfJironlnentaL

Prien Ly Synthetic Lubricant n Ifrol1'l ratm

REGULARS

There IS an Increasing interest in the

development of alternative lubricating oils from

renewable resources as compared to non­

renewable, non-biodegradable petroleum-based

flUids. Considerable research efforts have been

spent on the use of renewable fats and oils

partICularly on vegetable oils as alternative base

stocks In lubncatlng oil formulation. However, its

inherent characteristics such as inadequate

OXidative stability, poor corrOSion protection, and

poor low temperature performance have been

recognized as major impediments to their

potential use as lubricants. However, many

studies have indicated ways that can be applied

to overcome these problems including structural

modifications via epoxidation or

transesterification.

In thiS study, a new palm based lubncant was

synthesized via chemical transestenflCation of

palm based methyl esters With

trimethylolpropane, (TMP). Both palm oil and

palm kernel oil methyl esters were used as the

starting materials and sodium methoxide as the

catalyst. A new analytical technique using gas

chromatography was developed to analyze the

reaction products: TMp, palm based methyl

esters, monoesters, dlesters and triesters. The

kinetics study on transesterificatlon established

that the reactions occurred via three stepwise

and reversible reactions. The lubncatlon

properties of palm oil-based TMP esters

Indicated good potential as base stock In

biodegradable lubricant formulation. The

properties such as ViSCOSity, VI, wear and friction

properties are comparable to commercial

hydraulic fluids. The pour point (PP) problem

associated with the saturation level in palm oil

was resolved, as the pour point was successfully

improved to -32·C in high oleiC palm based TMP

With proper additives, it is believed that the new

formulated high oleic palm 011 TMP ester will

offer a Wide vanety of applications: hydraulic

flUids, fire resistant fluids, metalworking fluids,

and general lubricating oils. Its unique chemistry

offers excellent oxidative and thermal stability,

superior low temperature behaViour, and

biodegradability.

R.·\t·ur,·h r:.,lu/J;uml :!IJ03.

Tt'/: +603 8946 6291

E-mail: mbi..h@.·ng.lIl.m.t.JII.m).

SMS GatewayInterfaceAbdul Rahman Bin Ramli, Tuan Syed Abdul Rahman Bin Syed Mohamed and Beh Kok Siang

GSM Short-Message-Service (SMS) is common

text-base communication method using mobile

phone This prOject provides a low cost solution

for remote monitoring and controlling via SMS.

The system can be setup In any location WIth

GSM coverage in short duration. Various sensors

and control instrument can be interfaced into

the system. Motion sensors, temperature, alarm,

water level are examples of sensors that can be

interfaced to the system.

The system can automatically send feedback to

person-in-charge or a central data collection

cen er VIa SMS In case any defined condition

occurs. Instruments such as heaters, latches,

motors, bells, lights and computers can be

con rolled from a remote location. Commands

can be pre-defined and then sent via SMS to the

system to check latest status. Secunty ISSUes are

also being taken care of via a phone number

that is pre-defined can access the system. Once

the system receives SMS Commands from an

authorized user, the system will perform the

necessary command to control the vanous

instruments connected to the system. Full lOGs

are recorded in the computer that provides all

the necessary details for every Incoming and

ou gOing SMS.

lH\ 'ER 1'11\011 /I'lt Nt' «" Ir, f), ,df/I'IIl"1I/( 1''''/ ,,, 2(/0.'

If/lelligell1 Systems & Robo/;cs LabOTO/ory (/SRL)IlIs/;/llIe ofAdval/ced Techl/ology (fTMA)UI/;\I/1r,\'il; PlItra Malaysia43400 UPM. Serdal/g. Selal/gorMalays;a

Tel: +6038946 7532£-moi/: arr@plllm 11/1llUc111.R1):

Synth sis UPM R&D DIgeSt. tssve 3. 4th Quarter (Dec.2oo3) • ~

Page 10: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

Deparl/l/J!I/I ojChl//l/iml & Em: Ellgilll/erillgFal'llll)' ofEllgilleerillgUllil'ersili Pllim Malaysia43400 UPM. Serdallg. SelmJlIorMalaysi"

Engineering, University Putra Malaysia, have beenvery impressive and encouraging. Being first timeproduced in MalaYSia, carbon nanotubes producedin our labs using our new design of CVD haveshown very high quality and astonishinglyconsistent, and comparable With other researchersworldWide who produced carbon nanotubes. Othercarbon nanomaterials produced include carbonnanoflbers, carbon nanoparticles, and a newmaterial we named carbon nanoporous balls. @

Tel: +60389466268E-/l/ail: fcli;bJ71/@ ,'IJjUIP/IIA,dll.llJ)·

SI \I (" tlllm,,£: 1/, t lroll \lie rrJ\c"rJ!'t"

/,hofO""If,h 0/ C",bo!J \,lIlt l l""l"(Sri / -011111101111"', \ til ,Iii til" If')

2 \'r \J I'/mloural,/r,,/ ('0""/111 \tII'"lib, 1\

(," ItKI ndll/IIllt'lI, , II"("/.i'" \\' ,,\;n~ ,11(' (4\'/) 1I1l11

rl~ \ll11tel':l (II II bll"dl, '" tit, Cllrl'OJt '·alloWh, \\111",,, f tI

I" II" "\ 1>

WINI ER 1/""11 "'/II ,~IIIIII 1111" ,k/('II,",' ,~

7jdll/tll(I~1 \II..,/,[\ (1/1\\1 I""nl 11'1'1 Nn/'/Ir<'I,,~ /)('\"'/11/"11"111 I '/'"\1I;'1Il _I/O.!

50 far, the outcomes of this research at theDepartment of Chemical and EnVIronmental

than steel but it is 6 times lighter. ThiS researchrequires the setting up of an equipment called theChemICal Vapour Deposition (CVDl unit. As thistype of research did not exist in Malaysia before,such equipment {i.e. CVDl was not available.Therefore, as a start, the Idea of bUYing the CVDwas a first choice, however the exorbitant cost ofthe equipment {- RM 900kl besides the excessivetime taken to deliver It from overseas have spurredthe group of researchers to develop their owndesign of the CVD and build it from locally availablematerials. Thus, the cost {- RM 100kl and time ofthiS research have been dramatically reduced.

Not only to speak of the reduction of cost and timeachieved at UPM, the availability of the CVD willboost the research in the field of nanotechnology inMalaysia as this will encourage lots of researcherswho lack the facility and funding to run and startsimilar researches. A Malaysian patent has beenfiled for the UPM CVD. We look forward toindustrial collaboration to further enhancenanotechnology R&D for the country.

RESEARCH UPDATE

There are many methods of producing thesenanomaterials, including electric arc discharge, laserevaporation, chemical vapor deposition (CVDl, andplasma-enhanced CVD, among many others.Among these, CVD seems to be the most promisingmethod for possible industrial scale-up due to therelatively low growth temperature, high yields andthe high purities that can be achieved. Thesenanomaterials have a range of promising properties,including unique mechanical and electrical behaviorand are under Investigation for a wide range ofpractical applICations such as field emission displays,hydrogen storage, nanocomposites, and fuel cells.

Though in Malaysia this kind of research IS relativelynew, and if no initiative is taken by the researchershere, Malaysia will be leh behind in this field oftechnology and will be totally dependent on foreignsuppliers for its needs in this area. This research onnanotechnology is the door for many coming andpromising technologies and their enormousbeneficial applications. In order to follow up withthe existing global trend in technology, theDepartment of Chemical and EnVironmentalEngineering, Universiti Putra Malaysia, initiated aresearch on carbon nanotubes production inOctober 2001. Carbon nanotubes and carbonnanofibres are the building blocks ofnanotechnology research. Carbon nanotubes canexhibit tensile strength of upto 100 times greater

Research on new materials technology is attractingthe attention of researchers all over the world.Developments are being made to improve theproperties of the materials and also to findalternative precursors that can bestow desirableproperties on the materials. Great interest hasrecently developed in the area of nanostructuredcarbon materials. Carbon nanostructures aregaining considerable commercial importance withInterest growing rapidly over the decade or so sincethe discovery of Buckminster fullerenes, carbonnanotubes, and carbon nanofibers.

Design and Fabrication of CVD (Chemical Vapour Deposition)

for Nanotechnology Research(Carbon Nanotube and Carbon Nanofibre Synthesis)Fakhru'I-Razi Ahmadun, Muataz Ali Atieh, Iyuke Sunny Esayegbemu,

Maan Fahmi Alkatib, Dayang Radiah Awang Biak,

Abdul Ghani Liew Abdullah, Faizah Mohd Yasin,

Mohd Halim Shah Ismail and Abdul Hamed Belal Danna

REGULARS

tla.lI/).

Pest

Deparl/l/em ojForest MWIlI 'e/l/elJ(FaCility ojFares,,')'Ulli"e/",'iri Pltfl'll Malaysia434()() UPM. SerdClI/g. SI'IdllgorMalaysia

\/a"" li'lI/ 1'llI/ll I'ml' • /lOll II( I'Il//'I'~I I"/Ild /I'll N,,,wtll,·Ii< tlI/P"".I,11/""11111" 2/JlII

Thus 5pltNPV has the potential to be commerciallydeveloped for biopesticide of armyworm, S. lilura,~

Synthesis UPM R&D Digest. Issue 3, 4th Quarter (Dec. 2003) ~

The result shows that mortality significantlyIncreased by 47% from 53% when the larvae werereared at 20"C to 100% at 30 and 35"C. The LTsavalues decreased from 24 to 6 days. All virussuspension used in the experiment inducedmortality to more than 90% except in pH 11, whereit was 64% and 100% mortality was recorded frompH 7. The ITsa increased to about four folds whenthe pH of suspension was increased from 7 to 11.5pltNPV was, however, adversely affected byUV-Iight. Laboratory and field trials usmgUV-protectants show that the virus remainedinfective and subsequently gave a comparable yieldof Brassica rapae as compared With that of thechemically treated crop.

In this study an armyworm Spodoptera Iirura(Fabricius) {lep., Noctuidael, an important pest ofmany crops including cruciferous vegetables, wasselected. The virus, coded as 5pltNPV. originallyisolated from Telong, Kelantan, was tested for itsInfectivity to the armyworm at differenttemperature, pH and ultraviolet radiation.

This research reveals the potential of anucleopolyhedrovirus for development of abiopesticide for managing cruciferous vegetablepests.

Development of Biopesticide for

Managing CruciferollSVegetableAhmad Said Sajap,Mohammad Abdul Bakir,

Hussan Abdul Kadir and

Norani Abdul Samad

Page 11: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

A Glance at Research Inventions & Innovations at UPM'COlllilll/t·(ljrtJm Issue 1, 3rtl Q/U,rter (Sept.2oo3)...

Faculty' ResearchNo. Institute Researcher Innovation Cluner Project Numbe-t Alloution

29 AgrICUlture MOOd. RaZiIsmaII Preose control of lmgaoon. nutlltion and microdlmate for hK)h value AFF 01.()2.()4.{)()82 EAOOl RM I56.000vegetables in soilless culture ~ng automation and computer control

30 Agriculture Mohd. Rldzwan Abd. Hahm Commercial production of a complete nce straw ·based feed for intensIVe AFF 01·02..()4-0083 EAQOl RM255.000ruminant productIon

31 Agriculture Mood Yusof Hussein Enhanong biological control of Asiatic corn borer through habitat and MF 01.()l.()4..()4{)() EAOOl RMl42.000vegetative drversiflGitlOn

32 Agorulture RadzJah bmtl Othman tmproved y.eId of onlOfl (Aftium ascokll'llum) through combined inocuiatlOll BAS 01.()2.()4.Q098 EAOOl RM126,OOOWIth rhtzobactella and arbU5CUlar mycorrhlla

33 Agncunure Radziah bin.. Othman Enhancement of S\Wet potato quaijty through combtned appbcation of MF 01.()2-G4.Q097 EAOOl RM191.000bacterlil and arbuscular '"'JCOfThiza on organically amended soil

34 AgrKuhure RIta Mohamad Development 01 re51S1ance In l.he cocoa mInd HeIopel1lS thervora MF 01.()2.()4.()101 EACIOI RMISO,400

35 Agric;ulture Rosenanl blntl Abu Bakar Improvement of production system for organk leafy and frUit vegetables MF 01-02·Q4.()104 EACIOI RM135.880

36 AgrICulture Rosenam blntl Abu Bakar low input lertllrzation management 10 enhance soli productIVity and grain AFF 01·02·04-0103 EAOOl RM291.000corn )'Ields

37. Agnculture Rosh Mohamad The effect of herbICides on performance of V'eSlCUlar arbuscular o1.()2-G4-Q687-EAOO1 RMOmycornzhal fungi

38 Agriculture Saleh bin KadzlITllt1 In Vitro propagauon and con5efVatlOO of indigenous ordud~ AFF 09-02.()4..()448 EAOOl RlvtI27.000

39 Agnculture Sanah Meon OeYeIopment of an effectNe formulation techrOogy and delivery of BAS 01.()2.()4.()109 EAOOl RMl99.000blocontrol agents for the control of soil·borne dISeaSeS

40 Agriculture Shamshoddin bm Jusop AmeloratlOll of soil acidrty usmg organK-based fertilizers and natural silicate MF 01.()2.()4-QS03 EAOOl RM1SO.000for cocoa gro......th

41 AgrICulture 5hamshuddln bin Jusop Allevialion of acid soil infertihty in the Kemaslrl- Semarak IADP for MF 01.()l·04·0111 EAOOI RM147.000rice cultivallon

42 Agriculture Ihanlah Khandah S)'d Muhamm.>l Development 01 Hydrolysed Vegetable Protem from Winged Bean MF 03·02-04-0416 EAOOI RM6S.240

43 Agriculture Sitl Kajar Ahmad Improvement of commeroal npemng technology of Cavendish banana MF 01.()2.()4.()115 EACIOI RMI96,OOO

44 Agnculture SrtJ lauyah Darus AceumulatJOn Of Heavy Metal Resdeus In leafy And Root Vegetables Grown MF 01.()2.()4-()490 EACIOl RMI80,OOOOn DIfferent Soli Types In Johor

45 Agriculture 5l1J 2auyah Darus DlSlributJon of heavy metals and poIyaromatic hydrocarbon In the esIuanne MEE 01.()2-(14.()1IB EAOOl RMI85.000ecosystem along the larut·matang to Krian coastline. Perak

46 Agllculture 5yed Omar 5yed Rastan Control of ·Padi Angin" ~eeay Rt<e) mfestatron using growth regulators MF 01-QZ-()4.()402 EAOOl RMl99,OOOand anaerobic direct seeding techniques

47 Agriculture Thohirah Abdullah Commercial environment-eontrolled produetJon of Indigenous ornamental AFF 01·02-04-Q517 EAOOI RMl66,OOOzinglbers for potted and cut-flower production

48 Agriculture laharah Abdul Rahman Sustainable grain maIZe prod Ion ,n agroferestry system AFF 01.()2-Q4.()126 EACIOI RM175.620

49 Agriculture lamal AbIdJn MIor Ahmad StLldtes on Cladosponum-PhyIlos1.icta foliar disease complex in banana of 'All 01.()2-G4-Q127 EAOOI RM101.000lfI'lproved management

50 Agrlculture lakana bin Wahab 5electJon and charac:teriu!lOO of maize genotypes for water stress (drought) MF 01.()2.()4.()129 EAOOI RMI68.000tolerance

51 Agnculture Zulkifli HJ. Shamssuddln Enhancement In Yield Of Oil Palm Inoculated Wllh Plant Growth Promoting MF 09-02.()4.()497 EAOOl RM270,OOOROoba<1er~ (PGPR)

52. Agflculture Zulkiflr rdrus Alleviation of heat stress related problems in brOiler chickens through MF 01·02·04·0405 EAOO1 RM167.000nutnllonal manipulatIOn

53 Computet Soence and Abdul Az,m b. Abd Ghani The Development of Web-Based Software Certifteatlon Model ITM 04-02.()4.()175 EAOOl RM225,OOOInformaooo Technology

54 Computer Soenct and Abu Bakar Md. Sultan Hybrid GenetIC AIgonthm for University Class Ttmetabhng Q4.()2.Q4·0802·EAOOl RMOInlormaooo T«hnokIgy

55 Computer S6ence ¥'Id Mohd. Hasan b. Selamat Knowledge Managementlfl Collaborative Enwonrnent ITM 04-02..()4.()182 EAOOl RM231.000Informatioo TedmIogy

56 Economics and Abu Soflan bin Yaacob Study of the effecttveness and compliance on cntlCal areas In self·assessment EMA 0S.()2.()4.()189 EAOO1 RM77.040Management tax syslem

57. Economics and Ahmad lainuddin Abdullah Sunk Cost, entry and extt bamers in selected manufaclunng industry EMA 05-Q2-G4-Q531 EAOOI RM107,OOOManagement

5' Economics and Ahmad Zubaldl Baharumshah Trade, Current Account and the Exdlange Rates In Asian 6 EconomIes EMA OS·02-04-0532·EAOOl RMI50.260Management (Singapore, Thailand. MalaYSIii. Phlhppll''H!:S, lodonesra and Korea)

1 Data refers to IRPA RM.S (as of Sept Cycle 3, 2003): 379 EAR Grants. to be continued,..

tTht ~lJOfl ollht~ of the~ tnwntJOnS ¥lCI mov:atlV! rE"SW(h pl'oducu oJVaNbIr IOf~lJOflat UPM~ (ont.allrd in tht book-•R&D at UPM Crta~ Ntw frontlm of lnnovatlV! Re5Hrdl' • first EdilJOfl.

Eeb:. '''Yart Deep 5 1(Mw,~ Put*.hed by Rest.atth Managemtn.t Crntrt (RMCl UP!l.t. oJ'IallabIp!rom PublGuons & PromotIon Unit., AdrNNstralJOfl 8t.JIkWlg.l..IrWff\ltl Putra~. 43400 UfM.~ SNogor DaruI Ehsan.MaloY,w. Tel +60389466028.8946 6192, faa.. ..aJ3 &J.ll6539. e-lNII rsdwtfoOadnwI uprn.edu my

Synthesis. UPM R&D 0;9"", Issue 3, 4th 0ua"... IDec.2003)~

Page 12: Biofertilizer and Bioenhancerpsasir.upm.edu.my/136/1/synthesis_3.pdf · Dr. ZUlkini Hj. Sham'iudJin.Pmf~orSoilMinobiolog) (rum Agricullurc f:lCull) w""s declared the. Malll)'.;ianSociety

ilgem(>lll C~ue ,RMCR~ rch

UP ROD, ~ ISSue 3 4 h Quarter (Dec 2003)

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l1umber ", puhhc,u"", III ontem,llIIl",,1 ,11111 n.,lillnoil jl>umal ,1"'1'1lI appti,'aut'n ,.t , ,l,\rcu I,,'m 17 III ~1)('2 III 511 III Z003.P,.lenl an: ImpnrWnt m...·....h.Hlhlll hlf r.:~hling I~ Inlll of rt:' •...n.:h and hlr Ir"uhlcmn • nc,," In\ 'nllon, I""'ani' l.: untllCrl',alma..nch. In a nul hell..t p.ut:I1ll:.tn be (.'l1n lL.1cred 01' J cU"la '1 hctv.,:cl1 \OClct) and Ih~ m\~ntor. In C t.:hangt: lnr c()lnpl~t .

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Patents - transferring new inventions towardscommercial markets

-Synthesis--------

eportage