Blood samples

Kari KuulasmaaLuxembourg

9-11 April 2008

Uses of blood samples

Lipid measurementsTotal cholesterolHDL-cholesterol

GlucoseFatty acids and lipoproteinsBiomarkers

E.g. Refl. nutrition (vitamins, flavonoids..)

AntibodiesMarkers of infection and inflammation

DNA

Blood collection

FastingNot required for cholesterols8 h (overnight) for glucose

By certified phlebotomist Subject in sitting positionMinimal use of tourniquet

Cholesterol

Serum preferredClotting in room temperature for 30-60 minCentrifuging within an hourAnalysis preferably on the day of collection

or on refrigerated samples within 3 daysor frozen samples (max 6 months at -20, longer at -70)

Glucose

Tubes with citrate and fluorideCentrifuge within an hourAnalysis preferably on the day of collection

or on refrigerated samples within 3 daysor frozen samples (max 6 months at -20, longer at -70)

Change (%) of glucose concentration in whole blood

samples

Uchida et al. Clinica Chimica Acta 1988;172:101-108

Change (%) of glucose concentration in whole blood

samples

Citrate-fluoride mix

NaF/K-oxsalate

NaF/EDTA

Li-hep

Time (h)Väisänen et al. Kliin Lab 2008; 3-4

Laboratory procedures

Use accredited laboratoryGood direct enzymatic methods are availableCDC has a certification program for clinical diagnostic products for cholesterolsExternal quality control needs to be set up