carbon absorption in silages: a novel approach in silage ... · 2009 2010 development of a...
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Carbon absorption in silages: a novel approach in silage microbiology
Patrick SchmidtCharles O. Novinski
Maity Zopollatto
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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20102009
Development of a methodology for collecting all gases from fermentation
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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2012
2010
Schmidt et al. Greenhouse gas emissions from fermentation of corn silage
2009
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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20152010
2012
Souza et al. Does the silage absorb air during its fermentation? A lab trial on maize silages added with natamycin.
2009
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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2016
20152010
2012
2009
Pilot trial:Internal pressure andgas absorption
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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2018
2016
20152010
2012
2009
Ph.D. student dedicated to this
project
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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Background Li et al. (2017) reported an unknownbehaviour of negative pressureinside silos
“...partial CO2 dissolution in interstitial silage water”
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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BackgroundFirst acetogenic bacteria (Clostridium aceticum) described by Wieringa in 1936:
4H2 + 2CO2 CH3COOH + 2H2O
Harland G. Wood - entire career dedicated to CO2 fixation
Autotrophic Acetyl-CoA pathway of CO2 fixation only established in
the 90´s.
The “Wood-Ljungdahl Pathway” (1991)
Wood (1952) - first study with 13CO2 proving incorporation of CO2
during acetate formation
Drake (1994)
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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Background
(Rasgdale and Pierce, 2008)
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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Objectives
1. To evaluate the pressure inside the silos of maize silage.
2. To quantify the CO2 absorption throughout the storage period.
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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Material and methods Eight PVC silos (8.8 L) properly sealed. Maize forage (332 g kg-1 DM)Storage at controlled-temperature room (24 ±1 ºC) for 5 months
Silos attached to a 3-way valve and to a 1-L chambermade of low-density polyethylene, for collecting allgases. Chamber immersed in water
Pressure inside the silos assessed using a mercurycolumn manometer
Once the pressure become negative, four silos wereweekly fed with pure CO2. The gas was not forcedinside the silos!
After 147 days, silos were opened. Samples taken for pH measuring.
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
Material and methods
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Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
Material and methods
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Results
11 days of gas production3,235 ± 388 mL kg-1 DM
Increased negative pressureuntil 101 days for silos keptclosed-43 ± 2.6 mm Hg (-5.7 kPa)
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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Results19 supplies of CO2
5,590±2492 mL of CO2 were absorbed
Absorption continued throughout the trial!
pH closed - 3.76±0.03
pH CO2 - 3.63±0.02
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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Discussion
The Wood-Ljungdahl pathway is an energy-generating process of reducing CO2 to acetate under anaerobiosis
22 bacterial genera are described (Drake at al, 2008)
This process has never been described for silages!
In the future silos can become bioreactors fixing pollutant gases from farm activities (CO, CO2, N2O) into high quality nutritive compounds of feed
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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Liew et al. (2016)
Where can we go?
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná
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Conclusion
Maize silage seems to be able for absorbing and fixing CO2 by the Wood-Ljungdahl pathway.
Centro de Pesquisas em Forragicultura – Universidade Federal do Paraná